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The map label for this gene is grpE [H]

Identifier: 159896971

GI number: 159896971

Start: 514262

End: 514777

Strand: Direct

Name: grpE [H]

Synonym: Haur_0439

Alternate gene names: 159896971

Gene position: 514262-514777 (Clockwise)

Preceding gene: 159896970

Following gene: 159896972

Centisome position: 8.1

GC content: 48.26

Gene sequence:

>516_bases
ATGACGGAAGAAGTGCAAGCACAATCAACTGACGATACCAATCCTCAAGGCGATGCCGAAAGCCTGAACGAACGGATCGC
CACGCTCGAACGCGAAGTTGAAGAACACAAAACCAACTGGATGCGAGCAATTGCTGATTTCAAAAATTACAAACGCCGCA
CCGAAAGCGAACGCGAGGAGTTGATTCGCAATGCTAGCGCTGGTTTGATGTTGAAATTGCTTCCAGTGCTCGATGACCTG
CTCTTGGCGATGGGTCAAATTCCCGCTGAAATTGAAAACAATCAGTGGATTGGCGGCGTTAAACAAGTACAACGTAAATT
TGAAACGGTGCTTGAAGGCGCTGGCCTGCAACCAATTCCTGCCGTTGATGAAGAATTTGATCCGAATATTCACGAAGCAA
TTATGTTTGAAGAAGGCGACGAAGCGCAATCAAACAAAGTTGTGGCCGAATTACGCCGTGGCTATAAGCTTGGCGAACGG
GTCTTGCGTCCAACGGTCGTCAAAGTCGGCAAGTAG

Upstream 100 bases:

>100_bases
GGCGATTTATATGGCGAACGCAAAATAGAACCATCAGAATAGTGCTAGACGCTAGGCTGTGATCATACAAGTTTGATCTT
TACGGAGGTACGGAGTTCTC

Downstream 100 bases:

>100_bases
GAGCAACTAGTCAAAGCATCGTTTTAAGTGGCATTGCTGCCGTGATTGGCCGTCGCTTAGCGATGCTTTGTCGCTGTCTG
TGGAGCACGAGCTATATGGG

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MTEEVQAQSTDDTNPQGDAESLNERIATLEREVEEHKTNWMRAIADFKNYKRRTESEREELIRNASAGLMLKLLPVLDDL
LLAMGQIPAEIENNQWIGGVKQVQRKFETVLEGAGLQPIPAVDEEFDPNIHEAIMFEEGDEAQSNKVVAELRRGYKLGER
VLRPTVVKVGK

Sequences:

>Translated_171_residues
MTEEVQAQSTDDTNPQGDAESLNERIATLEREVEEHKTNWMRAIADFKNYKRRTESEREELIRNASAGLMLKLLPVLDDL
LLAMGQIPAEIENNQWIGGVKQVQRKFETVLEGAGLQPIPAVDEEFDPNIHEAIMFEEGDEAQSNKVVAELRRGYKLGER
VLRPTVVKVGK
>Mature_170_residues
TEEVQAQSTDDTNPQGDAESLNERIATLEREVEEHKTNWMRAIADFKNYKRRTESEREELIRNASAGLMLKLLPVLDDLL
LAMGQIPAEIENNQWIGGVKQVQRKFETVLEGAGLQPIPAVDEEFDPNIHEAIMFEEGDEAQSNKVVAELRRGYKLGERV
LRPTVVKVGK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=174, Percent_Identity=27.5862068965517, Blast_Score=67, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24653432, Length=176, Percent_Identity=27.8409090909091, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 19371; Mature: 19240

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEEVQAQSTDDTNPQGDAESLNERIATLEREVEEHKTNWMRAIADFKNYKRRTESEREE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIRNASAGLMLKLLPVLDDLLLAMGQIPAEIENNQWIGGVKQVQRKFETVLEGAGLQPIP
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
AVDEEFDPNIHEAIMFEEGDEAQSNKVVAELRRGYKLGERVLRPTVVKVGK
CCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEEVQAQSTDDTNPQGDAESLNERIATLEREVEEHKTNWMRAIADFKNYKRRTESEREE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIRNASAGLMLKLLPVLDDLLLAMGQIPAEIENNQWIGGVKQVQRKFETVLEGAGLQPIP
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
AVDEEFDPNIHEAIMFEEGDEAQSNKVVAELRRGYKLGERVLRPTVVKVGK
CCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA