Click here to switch to the map view.

The map label for this gene is metG [H]

Identifier: 158423799

GI number: 158423799

Start: 2491793

End: 2493373

Strand: Direct

Name: metG [H]

Synonym: AZC_2175

Alternate gene names: 158423799

Gene position: 2491793-2493373 (Clockwise)

Preceding gene: 158423798

Following gene: 158423802

Centisome position: 46.4

GC content: 65.65

Gene sequence:

>1581_bases
TTGTTCGGACGTGTCCCGATGTCTTCCAAGCGGCCCTTCTACATCACCACCGCCATCGCCTATCCCAACGGCGTGCCGCA
CATGGGCCACGCGTATGAGGCGATCGCCACCGACTGCATCGCCCGCTTCAAGCGGCTCGACGGCTATGACGTGCGCTTCC
TCACTGGCACGGACGAGCATGGCATCAAGATGCTCCAGACCGCCCAGAAGGCGGGCCTGACGCCGCGCGAGCTGCTGGAC
CGCAACGTGCCCGCCTTCAAGGCCATGGGCCCGGCCTTCAACCTCTCCAATGACGACTTCATCCGCACCACGGAGGAGCG
GCACATCAAGTCGTCGCAGGCCATCTGGGAGCGCATGGCGGCGAAGGGGGACATCTACAAGTCCACCTATTCCGGCTGGT
ATTCCGTGCGCGACGAGGCCTATTACGCCGAAGACGAGACCACGGTGAACGAGCAGAACGTCCGGCTCGGCCCGCAAGGC
ACGCCGGTGGAATGGGTGGAGGAGGAGAGCTATTTCTTCCGCCTCTCCGCCTATCAGCAGAAGCTGCTCGACTTCTACGC
CGCAAATCCCGGCTTCATCATGCCGGAGGCCCGGCGCAACGAGGTGGTGAGCTTCGTCTCTGGCGGCCTGCAGGATCTCT
CCATCTCGCGCACCACCTTCGACTGGGGCATTCCGGTGCCCGGCGATCCGGCGCACATCATGTATGTGTGGGTGGACGCG
CTGACCAACTACATCACCGCGCTCGGTTATCCCGACACGGATGCGGAGCTGTTCCGCAAGTTCTGGCCGGCGGACCTGCA
CGTCATCGGCAAGGACATCATCCGCTTCCATGCGGTCTATTGGCCTGCCTTCCTGTGGTCGGCGGGGCTGGAGCCGCCCA
AGCGCGTGTTCGGCCACGGCTTCCTGTTCAACCGCGGGGAGAAGATGTCGAAGTCGGTCGGCAATGTCGTCGATCCGTTC
GGGCTCGTGGAGACTTATGGCGTCGACGCCACGCGCTATTTCTTCCTGCGTGAAGTCTCCTTCGGCCAGGACGGCTCCTA
CAGCCACGAGGCCATCGTGAACCGCATGAATGCGGATCTCGCGAACGACCTCGGCAACCTCGCCCAGCGCTCGCTGTCGA
TGATCTCGAAGAATCTCGACGGCGTCGTGCCGGAGCCCGGCCCGCTCTCACCGGAGGACGAGGCCATCCTCGCGCTCGCG
GACGGCATGCTGGAGAAGGCCCGCGAGGCCATGGAGGTGCAGGCCATCCACCAGTATCTCGCCGCCGTCTGGGCGGTGGT
GGCGGAGGCCAACCGCTATTTTGCGGCTCAGGCGCCCTGGGCCCTGCGCAAGACCGATCCCAAGCGCATGGAAACGGTGC
TCTGGACCACGGCCGAGGTGATCCGCCAGGTCGCCATCCTCGCCCAGCCGGTAATGCCGGAGAGCGCGGGCAAGATGCTC
GACCTGCTGGTGGTGCCGGCGGAGCAGCGTGGCTTCGACGTGCTGGGCGGCACCTTCCGCCTGCCCGGCGGCACCGCTAT
CCCGGCGCCGACCGGTGTTTTCCCGCGCTATGTGGAGCCGGAAGCGCCCGCCGCCGGCTGA

Upstream 100 bases:

>100_bases
CTACAATCTTGACCGCAAGGCGCTGGTTTTTCGGATTTTCACCGAGCTCGCGGAGGCCGCGCGCGCCTGAAATGGGCGCG
GCCCGCCTCCCGCCGGTCTT

Downstream 100 bases:

>100_bases
ACGTCGGCGATCAGAAAGCCAAAATGACGAAGGCCCGGCGCGCTGCGCCGGGCCTTCGCTTTTGGGGCGCGCCTACGTGC
AGATCACTCGTTGATCGAGG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MFGRVPMSSKRPFYITTAIAYPNGVPHMGHAYEAIATDCIARFKRLDGYDVRFLTGTDEHGIKMLQTAQKAGLTPRELLD
RNVPAFKAMGPAFNLSNDDFIRTTEERHIKSSQAIWERMAAKGDIYKSTYSGWYSVRDEAYYAEDETTVNEQNVRLGPQG
TPVEWVEEESYFFRLSAYQQKLLDFYAANPGFIMPEARRNEVVSFVSGGLQDLSISRTTFDWGIPVPGDPAHIMYVWVDA
LTNYITALGYPDTDAELFRKFWPADLHVIGKDIIRFHAVYWPAFLWSAGLEPPKRVFGHGFLFNRGEKMSKSVGNVVDPF
GLVETYGVDATRYFFLREVSFGQDGSYSHEAIVNRMNADLANDLGNLAQRSLSMISKNLDGVVPEPGPLSPEDEAILALA
DGMLEKAREAMEVQAIHQYLAAVWAVVAEANRYFAAQAPWALRKTDPKRMETVLWTTAEVIRQVAILAQPVMPESAGKML
DLLVVPAEQRGFDVLGGTFRLPGGTAIPAPTGVFPRYVEPEAPAAG

Sequences:

>Translated_526_residues
MFGRVPMSSKRPFYITTAIAYPNGVPHMGHAYEAIATDCIARFKRLDGYDVRFLTGTDEHGIKMLQTAQKAGLTPRELLD
RNVPAFKAMGPAFNLSNDDFIRTTEERHIKSSQAIWERMAAKGDIYKSTYSGWYSVRDEAYYAEDETTVNEQNVRLGPQG
TPVEWVEEESYFFRLSAYQQKLLDFYAANPGFIMPEARRNEVVSFVSGGLQDLSISRTTFDWGIPVPGDPAHIMYVWVDA
LTNYITALGYPDTDAELFRKFWPADLHVIGKDIIRFHAVYWPAFLWSAGLEPPKRVFGHGFLFNRGEKMSKSVGNVVDPF
GLVETYGVDATRYFFLREVSFGQDGSYSHEAIVNRMNADLANDLGNLAQRSLSMISKNLDGVVPEPGPLSPEDEAILALA
DGMLEKAREAMEVQAIHQYLAAVWAVVAEANRYFAAQAPWALRKTDPKRMETVLWTTAEVIRQVAILAQPVMPESAGKML
DLLVVPAEQRGFDVLGGTFRLPGGTAIPAPTGVFPRYVEPEAPAAG
>Mature_526_residues
MFGRVPMSSKRPFYITTAIAYPNGVPHMGHAYEAIATDCIARFKRLDGYDVRFLTGTDEHGIKMLQTAQKAGLTPRELLD
RNVPAFKAMGPAFNLSNDDFIRTTEERHIKSSQAIWERMAAKGDIYKSTYSGWYSVRDEAYYAEDETTVNEQNVRLGPQG
TPVEWVEEESYFFRLSAYQQKLLDFYAANPGFIMPEARRNEVVSFVSGGLQDLSISRTTFDWGIPVPGDPAHIMYVWVDA
LTNYITALGYPDTDAELFRKFWPADLHVIGKDIIRFHAVYWPAFLWSAGLEPPKRVFGHGFLFNRGEKMSKSVGNVVDPF
GLVETYGVDATRYFFLREVSFGQDGSYSHEAIVNRMNADLANDLGNLAQRSLSMISKNLDGVVPEPGPLSPEDEAILALA
DGMLEKAREAMEVQAIHQYLAAVWAVVAEANRYFAAQAPWALRKTDPKRMETVLWTTAEVIRQVAILAQPVMPESAGKML
DLLVVPAEQRGFDVLGGTFRLPGGTAIPAPTGVFPRYVEPEAPAAG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=530, Percent_Identity=38.8679245283019, Blast_Score=351, Evalue=1e-96,
Organism=Homo sapiens, GI14043022, Length=553, Percent_Identity=25.6781193490054, Blast_Score=154, Evalue=3e-37,
Organism=Escherichia coli, GI1788432, Length=500, Percent_Identity=26.6, Blast_Score=196, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI212645096, Length=526, Percent_Identity=34.6007604562738, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI71992781, Length=416, Percent_Identity=33.4134615384615, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17540794, Length=527, Percent_Identity=25.2371916508539, Blast_Score=143, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6321610, Length=530, Percent_Identity=33.2075471698113, Blast_Score=286, Evalue=7e-78,
Organism=Saccharomyces cerevisiae, GI6321704, Length=526, Percent_Identity=23.574144486692, Blast_Score=124, Evalue=3e-29,
Organism=Drosophila melanogaster, GI21358483, Length=518, Percent_Identity=37.0656370656371, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24655311, Length=564, Percent_Identity=25.886524822695, Blast_Score=146, Evalue=4e-35,
Organism=Drosophila melanogaster, GI21355409, Length=183, Percent_Identity=25.6830601092896, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58664; Mature: 58664

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGRVPMSSKRPFYITTAIAYPNGVPHMGHAYEAIATDCIARFKRLDGYDVRFLTGTDEH
CCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHH
GIKMLQTAQKAGLTPRELLDRNVPAFKAMGPAFNLSNDDFIRTTEERHIKSSQAIWERMA
HHHHHHHHHHHCCCHHHHHHCCCCHHHHCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHH
AKGDIYKSTYSGWYSVRDEAYYAEDETTVNEQNVRLGPQGTPVEWVEEESYFFRLSAYQQ
HCCCHHHHHHCCCEEECCCEEECCCCCCCCCCCEEECCCCCCHHHCCCCCCEEEHHHHHH
KLLDFYAANPGFIMPEARRNEVVSFVSGGLQDLSISRTTFDWGIPVPGDPAHIMYVWVDA
HHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHCEEEEEEEECCCCCCCCCCEEEEHHHHH
LTNYITALGYPDTDAELFRKFWPADLHVIGKDIIRFHAVYWPAFLWSAGLEPPKRVFGHG
HHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC
FLFNRGEKMSKSVGNVVDPFGLVETYGVDATRYFFLREVSFGQDGSYSHEAIVNRMNADL
CEECCCHHHHHHHCCCCCCCHHHHHCCCCHHHEEEEEEECCCCCCCCCHHHHHHHHHHHH
ANDLGNLAQRSLSMISKNLDGVVPEPGPLSPEDEAILALADGMLEKAREAMEVQAIHQYL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVWAVVAEANRYFAAQAPWALRKTDPKRMETVLWTTAEVIRQVAILAQPVMPESAGKML
HHHHHHHHHHHHEEEECCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
DLLVVPAEQRGFDVLGGTFRLPGGTAIPAPTGVFPRYVEPEAPAAG
EEEEECCCCCCCEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFGRVPMSSKRPFYITTAIAYPNGVPHMGHAYEAIATDCIARFKRLDGYDVRFLTGTDEH
CCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHH
GIKMLQTAQKAGLTPRELLDRNVPAFKAMGPAFNLSNDDFIRTTEERHIKSSQAIWERMA
HHHHHHHHHHHCCCHHHHHHCCCCHHHHCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHH
AKGDIYKSTYSGWYSVRDEAYYAEDETTVNEQNVRLGPQGTPVEWVEEESYFFRLSAYQQ
HCCCHHHHHHCCCEEECCCEEECCCCCCCCCCCEEECCCCCCHHHCCCCCCEEEHHHHHH
KLLDFYAANPGFIMPEARRNEVVSFVSGGLQDLSISRTTFDWGIPVPGDPAHIMYVWVDA
HHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHCEEEEEEEECCCCCCCCCCEEEEHHHHH
LTNYITALGYPDTDAELFRKFWPADLHVIGKDIIRFHAVYWPAFLWSAGLEPPKRVFGHG
HHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC
FLFNRGEKMSKSVGNVVDPFGLVETYGVDATRYFFLREVSFGQDGSYSHEAIVNRMNADL
CEECCCHHHHHHHCCCCCCCHHHHHCCCCHHHEEEEEEECCCCCCCCCHHHHHHHHHHHH
ANDLGNLAQRSLSMISKNLDGVVPEPGPLSPEDEAILALADGMLEKAREAMEVQAIHQYL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVWAVVAEANRYFAAQAPWALRKTDPKRMETVLWTTAEVIRQVAILAQPVMPESAGKML
HHHHHHHHHHHHEEEECCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
DLLVVPAEQRGFDVLGGTFRLPGGTAIPAPTGVFPRYVEPEAPAAG
EEEEECCCCCCCEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]