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The map label for this gene is holB [H]

Identifier: 158423798

GI number: 158423798

Start: 2490701

End: 2491762

Strand: Direct

Name: holB [H]

Synonym: AZC_2174

Alternate gene names: 158423798

Gene position: 2490701-2491762 (Clockwise)

Preceding gene: 158423797

Following gene: 158423799

Centisome position: 46.38

GC content: 72.32

Gene sequence:

>1062_bases
TTGGCGCGTGCACCCGCCCCGAAGGACGACGGCGAGGCGCTGGAGGGCGATGTCCTGACCGGCGCGCCGCATCCGCGGCG
TCAGGATCGCCTGTTCGGTCATGCGGAAGTGGAAGCCGACCTCATGGCGGAATGGCGCTCGGGCCGCCTGCCGCACGGGC
TGCTGATCGGCGGGCCGGAAGGGGTGGGCAAGGCGACCATGGCCTACCGCATTGCCCGCTTCGTCCTGTCGGGCGGCGCC
GCGCCCGCCTCGCAGCACAGCTTCGATGTGCCGGAGAGCCACCCCGTCTGGCGGCAGGTGGCGGCGCTTTCCCATCCAGA
CCTCCTGGTGCTGCGGCGCGTGCCAGAGCCGGGCGAGGAGAAGATCCCGACCGTCATCCCCGCCGAGATGGTGCGCAAGG
TGCGCAATTTCTTCGGCGCCACGGCGGGTTCCGGTGGCTGGCGCGTGTGCATCGTGGACGCGGTGGATGATCTCAACGCC
TTCGGCGCCAATGCGCTGCTGAAGACGCTGGAAGAGCCGCCGCCGCGCGCGCTGTTCCTGCTGGTCACCCATGCGCCCGG
CCGCGTGCTGCCGACCATCCGCTCACGCACCCGGGCTGTGCGGCTGAAGCCGCTGGGCGGGGCGGAGGTGCTGGCGGCGC
TTGATCATCTGGCGCCGGCTCTGGCGCTGGAGGAGGCGGACCTGCCGGCGGCGGCCGCCGCCAGCGGGGGCAGCGTGCGG
CGGGCGATCGTATTGGCGCGGGGCGAGGGCATGGAGGTGCGCGCGGCCACCCAGCGGGCGCTGGAACAGTTGCCGCAGCC
GCGCATGGAGGAGCTTCACGCCCTCGGCGCGCGCTTGCAGGGCGACCGGGGCGACGGGCTCGACCTCTTTCTCGAGACGG
TCAGCGACTGGATCGCGGCGGAGGCCACCTCGGGCCTTGCCGGGCGGGCGCGCCTTGTCCGGCTTGGCGAGGTGTGGGAG
AAGGTGAGGCGAAGCGGCGCCGAGGCGGACATCTACAATCTTGACCGCAAGGCGCTGGTTTTTCGGATTTTCACCGAGCT
CGCGGAGGCCGCGCGCGCCTGA

Upstream 100 bases:

>100_bases
GCTGGTCCCCCCGGAAAGGCCGCTGTCGGCGAAGCCCGCCGCGACGCGCAAGCCGGCCGGAAGCGGCCGCCGCCGGACTC
GTTGAGCGAAGGAGCAGATC

Downstream 100 bases:

>100_bases
AATGGGCGCGGCCCGCCTCCCGCCGGTCTTTTGTTCGGACGTGTCCCGATGTCTTCCAAGCGGCCCTTCTACATCACCAC
CGCCATCGCCTATCCCAACG

Product: DNA polymerase III delta prime subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MARAPAPKDDGEALEGDVLTGAPHPRRQDRLFGHAEVEADLMAEWRSGRLPHGLLIGGPEGVGKATMAYRIARFVLSGGA
APASQHSFDVPESHPVWRQVAALSHPDLLVLRRVPEPGEEKIPTVIPAEMVRKVRNFFGATAGSGGWRVCIVDAVDDLNA
FGANALLKTLEEPPPRALFLLVTHAPGRVLPTIRSRTRAVRLKPLGGAEVLAALDHLAPALALEEADLPAAAAASGGSVR
RAIVLARGEGMEVRAATQRALEQLPQPRMEELHALGARLQGDRGDGLDLFLETVSDWIAAEATSGLAGRARLVRLGEVWE
KVRRSGAEADIYNLDRKALVFRIFTELAEAARA

Sequences:

>Translated_353_residues
MARAPAPKDDGEALEGDVLTGAPHPRRQDRLFGHAEVEADLMAEWRSGRLPHGLLIGGPEGVGKATMAYRIARFVLSGGA
APASQHSFDVPESHPVWRQVAALSHPDLLVLRRVPEPGEEKIPTVIPAEMVRKVRNFFGATAGSGGWRVCIVDAVDDLNA
FGANALLKTLEEPPPRALFLLVTHAPGRVLPTIRSRTRAVRLKPLGGAEVLAALDHLAPALALEEADLPAAAAASGGSVR
RAIVLARGEGMEVRAATQRALEQLPQPRMEELHALGARLQGDRGDGLDLFLETVSDWIAAEATSGLAGRARLVRLGEVWE
KVRRSGAEADIYNLDRKALVFRIFTELAEAARA
>Mature_352_residues
ARAPAPKDDGEALEGDVLTGAPHPRRQDRLFGHAEVEADLMAEWRSGRLPHGLLIGGPEGVGKATMAYRIARFVLSGGAA
PASQHSFDVPESHPVWRQVAALSHPDLLVLRRVPEPGEEKIPTVIPAEMVRKVRNFFGATAGSGGWRVCIVDAVDDLNAF
GANALLKTLEEPPPRALFLLVTHAPGRVLPTIRSRTRAVRLKPLGGAEVLAALDHLAPALALEEADLPAAAAASGGSVRR
AIVLARGEGMEVRAATQRALEQLPQPRMEELHALGARLQGDRGDGLDLFLETVSDWIAAEATSGLAGRARLVRLGEVWEK
VRRSGAEADIYNLDRKALVFRIFTELAEAARA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=221, Percent_Identity=32.1266968325792, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37845; Mature: 37714

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAPAPKDDGEALEGDVLTGAPHPRRQDRLFGHAEVEADLMAEWRSGRLPHGLLIGGPE
CCCCCCCCCCCCCCCCCEECCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEECCC
GVGKATMAYRIARFVLSGGAAPASQHSFDVPESHPVWRQVAALSHPDLLVLRRVPEPGEE
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCC
KIPTVIPAEMVRKVRNFFGATAGSGGWRVCIVDAVDDLNAFGANALLKTLEEPPPRALFL
CCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCCEEEE
LVTHAPGRVLPTIRSRTRAVRLKPLGGAEVLAALDHLAPALALEEADLPAAAAASGGSVR
EEECCCCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEE
RAIVLARGEGMEVRAATQRALEQLPQPRMEELHALGARLQGDRGDGLDLFLETVSDWIAA
EEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
EATSGLAGRARLVRLGEVWEKVRRSGAEADIYNLDRKALVFRIFTELAEAARA
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARAPAPKDDGEALEGDVLTGAPHPRRQDRLFGHAEVEADLMAEWRSGRLPHGLLIGGPE
CCCCCCCCCCCCCCCCEECCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEECCC
GVGKATMAYRIARFVLSGGAAPASQHSFDVPESHPVWRQVAALSHPDLLVLRRVPEPGEE
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCC
KIPTVIPAEMVRKVRNFFGATAGSGGWRVCIVDAVDDLNAFGANALLKTLEEPPPRALFL
CCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCCEEEE
LVTHAPGRVLPTIRSRTRAVRLKPLGGAEVLAALDHLAPALALEEADLPAAAAASGGSVR
EEECCCCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEE
RAIVLARGEGMEVRAATQRALEQLPQPRMEELHALGARLQGDRGDGLDLFLETVSDWIAA
EEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
EATSGLAGRARLVRLGEVWEKVRRSGAEADIYNLDRKALVFRIFTELAEAARA
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]