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The map label for this gene is tmk [H]

Identifier: 158423797

GI number: 158423797

Start: 2489978

End: 2490685

Strand: Direct

Name: tmk [H]

Synonym: AZC_2173

Alternate gene names: 158423797

Gene position: 2489978-2490685 (Clockwise)

Preceding gene: 158423796

Following gene: 158423798

Centisome position: 46.37

GC content: 72.32

Gene sequence:

>708_bases
ATGAGTTCCGCCCGCGGGCGCTTCATCACGCTGGAAGGCGGAGAAGGCACCGGAAAGTCCACACAGGCCCGCCGTCTCGC
CGCTGCCCTCCAGAAGGGCGGCCTCGAGGTGGTGCTCACCCGCGAACCCGGCGGCTCGGCGGGCGCGGAGGCGGTGCGCC
ATGTGCTCCTGTCAGGCGCGGCCAAGGACTTCGGGCCGCAGGGTGAGGCCCTCCTCTTCGCTGCAGCCCGGGCCGACCAT
GTGGACAGCCTCATCCGCCCCGCTCTGGCTGCCGGCAAGTGGGTGATCTGCGACCGCTTCATCGATTCCACGCGGGTCTA
TCAGGGCGCGGTCGGCGCTGTGCCCGGAGCGCTGCTGGACAGCCTCGAGTGGGTGGCCGCTGCCGAGGCGCGGCCGGACC
TCACGCTTGTGCTCGACGTGCCGCCGGAGGTGGGCCTTTCCCGGGCTGCCGGGCGCGGGGAGGGAGCAGACCGGTTCGAG
AGCGAGGGGCTTGCCTTCCACACCCGCGTGCGGGATGCCTTCCTCGCACTGGCGAAGGCTGATCCCGCCCGCTGTGTCAT
TGTGGATGCCTCGGGCGGCCCGGATGACGTTGCGGCGCTCGTCTGGTCCAGTGTCCGCGAACGGCTGGTCCCCCCGGAAA
GGCCGCTGTCGGCGAAGCCCGCCGCGACGCGCAAGCCGGCCGGAAGCGGCCGCCGCCGGACTCGTTGA

Upstream 100 bases:

>100_bases
CCGTGGGCACACTGTGGCAGCGGGCGCTCGACGGCGGCTGGACCGTCGCGGGCGACGCGGCGCGGGACCTCACCACGCAG
GTCATGGCGAAGTTCCGCAA

Downstream 100 bases:

>100_bases
GCGAAGGAGCAGATCTTGGCGCGTGCACCCGCCCCGAAGGACGACGGCGAGGCGCTGGAGGGCGATGTCCTGACCGGCGC
GCCGCATCCGCGGCGTCAGG

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MSSARGRFITLEGGEGTGKSTQARRLAAALQKGGLEVVLTREPGGSAGAEAVRHVLLSGAAKDFGPQGEALLFAAARADH
VDSLIRPALAAGKWVICDRFIDSTRVYQGAVGAVPGALLDSLEWVAAAEARPDLTLVLDVPPEVGLSRAAGRGEGADRFE
SEGLAFHTRVRDAFLALAKADPARCVIVDASGGPDDVAALVWSSVRERLVPPERPLSAKPAATRKPAGSGRRRTR

Sequences:

>Translated_235_residues
MSSARGRFITLEGGEGTGKSTQARRLAAALQKGGLEVVLTREPGGSAGAEAVRHVLLSGAAKDFGPQGEALLFAAARADH
VDSLIRPALAAGKWVICDRFIDSTRVYQGAVGAVPGALLDSLEWVAAAEARPDLTLVLDVPPEVGLSRAAGRGEGADRFE
SEGLAFHTRVRDAFLALAKADPARCVIVDASGGPDDVAALVWSSVRERLVPPERPLSAKPAATRKPAGSGRRRTR
>Mature_234_residues
SSARGRFITLEGGEGTGKSTQARRLAAALQKGGLEVVLTREPGGSAGAEAVRHVLLSGAAKDFGPQGEALLFAAARADHV
DSLIRPALAAGKWVICDRFIDSTRVYQGAVGAVPGALLDSLEWVAAAEARPDLTLVLDVPPEVGLSRAAGRGEGADRFES
EGLAFHTRVRDAFLALAKADPARCVIVDASGGPDDVAALVWSSVRERLVPPERPLSAKPAATRKPAGSGRRRTR

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis [H]

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787340, Length=187, Percent_Identity=42.2459893048128, Blast_Score=131, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018095
- InterPro:   IPR018094 [H]

Pfam domain/function: PF02223 Thymidylate_kin [H]

EC number: =2.7.4.9 [H]

Molecular weight: Translated: 24490; Mature: 24359

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSARGRFITLEGGEGTGKSTQARRLAAALQKGGLEVVLTREPGGSAGAEAVRHVLLSGA
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCC
AKDFGPQGEALLFAAARADHVDSLIRPALAAGKWVICDRFIDSTRVYQGAVGAVPGALLD
HHCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCHHCCCHHHHH
SLEWVAAAEARPDLTLVLDVPPEVGLSRAAGRGEGADRFESEGLAFHTRVRDAFLALAKA
HHHHHHHHCCCCCEEEEEECCCCCCCHHCCCCCCCCHHHHCCCCEEHHHHHHHHHHHHCC
DPARCVIVDASGGPDDVAALVWSSVRERLVPPERPLSAKPAATRKPAGSGRRRTR
CCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSARGRFITLEGGEGTGKSTQARRLAAALQKGGLEVVLTREPGGSAGAEAVRHVLLSGA
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCC
AKDFGPQGEALLFAAARADHVDSLIRPALAAGKWVICDRFIDSTRVYQGAVGAVPGALLD
HHCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCHHCCCHHHHH
SLEWVAAAEARPDLTLVLDVPPEVGLSRAAGRGEGADRFESEGLAFHTRVRDAFLALAKA
HHHHHHHHCCCCCEEEEEECCCCCCCHHCCCCCCCCHHHHCCCCEEHHHHHHHHHHHHCC
DPARCVIVDASGGPDDVAALVWSSVRERLVPPERPLSAKPAATRKPAGSGRRRTR
CCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]