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The map label for this gene is appA [H]

Identifier: 158423669

GI number: 158423669

Start: 2357659

End: 2359230

Strand: Direct

Name: appA [H]

Synonym: AZC_2045

Alternate gene names: 158423669

Gene position: 2357659-2359230 (Clockwise)

Preceding gene: 158423668

Following gene: 158423670

Centisome position: 43.91

GC content: 62.28

Gene sequence:

>1572_bases
ATGAAAGCGAAATTGCTCGGTACACTTGCGGCGTCCGTCGCGCTTTCCGTCCTCGCCGCGGGCGGCGCATTCGCCCAGCA
GGGCACGCCCAAGGAGGGCGGCCGCGCCAATGTGGTGATCCAGCCGGAGCCGCCGGGGCTCATGGTCGGCATCCTTCAGA
ATGCCCCGACCCAGATGGTCGCCGGCAACATCTACGAGGGCCTGCTGCGCTATGACGAGAAGCTGCAGCCGAAGCCCCAG
CTTGCCAAGTCCTGGACCATTTCCGAGGACGGGAAGACTTACACCTTCAAGCTCCAGCCCGGCGTGAAGTGGCATGACGG
CAAGCCCTTCACGGCGGCCGACGTGGTGTTCTCCGCCGACGTCTTCCTGCGCAAGACCAATCCGCGCACGCGCGCCTCGC
TCGCTTATGTGGAGAGCATCACCGCGCCGGACGACGAGACGGTGGTGTTCCAGCTGAAGCAGCCCTTCGGCCCCTTCATC
CGCATGTTCGAGGTCGGCACCATGCCCATGGTGCCCAAGCACATCTATGAGGGCACCGACTTCCTCACCAATCCGGCGAA
CAATACGCCCATCGGCACCGGTCCCTTCAAGTTCGGAGAATGGGTGAAGGGCTCCTATATCAAGCTCGTCAAGAACAAGG
ACTATTACGAGAAGGGCAAGCCCCATCTCGACGAGATCTACTGGCACATCATTCCGGATGCCGCGGCCCGCGCGGTCGCC
TTCGAGACCGGCAAGGTGGACATCCTGCCCGGCGGGTCGGTGGAGAATTTCGACATTCCCCGCCTCGCCAAGACGAAGAA
TGCCTGCATCACCGACAAGGGCTGGGAGTTCTTCGCGCCACAGTCCTGGCTCTGGTTCAACCATCGCGGCGGCCTGACGG
CGGACCCTCGCTTCCGCAAGGCGGTGTCCTACGCCATCGACCGCAACTTCGCGCTCGACGTGCTGTGGAACGGTATGGGC
GCTGTATCCGACGGTCCCTTCGCCCAGTCCACGTCCTTCCGCGATGACAAGGCGGTGGTGAAGTATCCCTACGATCCCGC
CAAGGCCAAGGCCCTGCTCAAGGAGATGGGCTACAAGGGCGAGCCGGTGCGCTTCACGCCCATGCCCTATGGCGAGACCT
GGCTGCGCTGGGCCGAGGCCATCAAGCAGAATTTGGAAGATGTCGGCATCAAGGTCGAGATGGTGAATACCGACGTCGCG
GGCTGGAACAAGAAGCTCGGCGACTGGGATTTCGATATCTCCATCACCTTCCTCTACCAGTATGGCGACCCGGCGCTGGG
CGTTTCCCGCGCCTATATCTCCTCCAACATTGCCAAGGGCTCGCCCTTCAACAATGTGGGCGGCTATTCCAACCCCGAGG
TGGACAAGCTGTTTGCCGAGGGCGCCGTCGGCTTCCCCGACAGCGAGCGCCAGAAGGCCTATTCCGCCGTCCAGAAGAAG
CTGACGGAGGATGCGGTCTATGACTGGCTCATCGACCTCAAGTTCCCGACGCTGACCCATTGCAACGTCAAGAACCTCAT
CACCACCGGCATCGGTGTGAATGACGGCTTCAAGGACGCCTGGATCGAGTGA

Upstream 100 bases:

>100_bases
ATCCCGAGTGGGCCGCCTTCCTGCGGCTCACCCGCGCCGTGGCGCCCGATTTCGAACACTGAAGGCTCAATCAACAATCC
TTCCAGGGGAACAAGCCGTC

Downstream 100 bases:

>100_bases
TCCGCCAGCCCCGAAACGGGCTTCGCGCAGCGCACCCGGGCGGCGGTTCCCCCTCCGCCGCCCCGTCGTTTCCGCCGACG
AGATCCGCCCTACATGCAGA

Product: ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MKAKLLGTLAASVALSVLAAGGAFAQQGTPKEGGRANVVIQPEPPGLMVGILQNAPTQMVAGNIYEGLLRYDEKLQPKPQ
LAKSWTISEDGKTYTFKLQPGVKWHDGKPFTAADVVFSADVFLRKTNPRTRASLAYVESITAPDDETVVFQLKQPFGPFI
RMFEVGTMPMVPKHIYEGTDFLTNPANNTPIGTGPFKFGEWVKGSYIKLVKNKDYYEKGKPHLDEIYWHIIPDAAARAVA
FETGKVDILPGGSVENFDIPRLAKTKNACITDKGWEFFAPQSWLWFNHRGGLTADPRFRKAVSYAIDRNFALDVLWNGMG
AVSDGPFAQSTSFRDDKAVVKYPYDPAKAKALLKEMGYKGEPVRFTPMPYGETWLRWAEAIKQNLEDVGIKVEMVNTDVA
GWNKKLGDWDFDISITFLYQYGDPALGVSRAYISSNIAKGSPFNNVGGYSNPEVDKLFAEGAVGFPDSERQKAYSAVQKK
LTEDAVYDWLIDLKFPTLTHCNVKNLITTGIGVNDGFKDAWIE

Sequences:

>Translated_523_residues
MKAKLLGTLAASVALSVLAAGGAFAQQGTPKEGGRANVVIQPEPPGLMVGILQNAPTQMVAGNIYEGLLRYDEKLQPKPQ
LAKSWTISEDGKTYTFKLQPGVKWHDGKPFTAADVVFSADVFLRKTNPRTRASLAYVESITAPDDETVVFQLKQPFGPFI
RMFEVGTMPMVPKHIYEGTDFLTNPANNTPIGTGPFKFGEWVKGSYIKLVKNKDYYEKGKPHLDEIYWHIIPDAAARAVA
FETGKVDILPGGSVENFDIPRLAKTKNACITDKGWEFFAPQSWLWFNHRGGLTADPRFRKAVSYAIDRNFALDVLWNGMG
AVSDGPFAQSTSFRDDKAVVKYPYDPAKAKALLKEMGYKGEPVRFTPMPYGETWLRWAEAIKQNLEDVGIKVEMVNTDVA
GWNKKLGDWDFDISITFLYQYGDPALGVSRAYISSNIAKGSPFNNVGGYSNPEVDKLFAEGAVGFPDSERQKAYSAVQKK
LTEDAVYDWLIDLKFPTLTHCNVKNLITTGIGVNDGFKDAWIE
>Mature_523_residues
MKAKLLGTLAASVALSVLAAGGAFAQQGTPKEGGRANVVIQPEPPGLMVGILQNAPTQMVAGNIYEGLLRYDEKLQPKPQ
LAKSWTISEDGKTYTFKLQPGVKWHDGKPFTAADVVFSADVFLRKTNPRTRASLAYVESITAPDDETVVFQLKQPFGPFI
RMFEVGTMPMVPKHIYEGTDFLTNPANNTPIGTGPFKFGEWVKGSYIKLVKNKDYYEKGKPHLDEIYWHIIPDAAARAVA
FETGKVDILPGGSVENFDIPRLAKTKNACITDKGWEFFAPQSWLWFNHRGGLTADPRFRKAVSYAIDRNFALDVLWNGMG
AVSDGPFAQSTSFRDDKAVVKYPYDPAKAKALLKEMGYKGEPVRFTPMPYGETWLRWAEAIKQNLEDVGIKVEMVNTDVA
GWNKKLGDWDFDISITFLYQYGDPALGVSRAYISSNIAKGSPFNNVGGYSNPEVDKLFAEGAVGFPDSERQKAYSAVQKK
LTEDAVYDWLIDLKFPTLTHCNVKNLITTGIGVNDGFKDAWIE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=488, Percent_Identity=27.2540983606557, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1789966, Length=429, Percent_Identity=30.0699300699301, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1789887, Length=507, Percent_Identity=27.6134122287968, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1787762, Length=432, Percent_Identity=27.5462962962963, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081878, Length=528, Percent_Identity=25, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787495, Length=516, Percent_Identity=22.4806201550388, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1787551, Length=455, Percent_Identity=23.2967032967033, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1789397, Length=479, Percent_Identity=24.0083507306889, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87082063, Length=443, Percent_Identity=21.8961625282167, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57961; Mature: 57961

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKLLGTLAASVALSVLAAGGAFAQQGTPKEGGRANVVIQPEPPGLMVGILQNAPTQMV
CCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCEEEEEECCCCCCEEEEEECCCCHHHH
AGNIYEGLLRYDEKLQPKPQLAKSWTISEDGKTYTFKLQPGVKWHDGKPFTAADVVFSAD
HHHHHHHHHHHHHHCCCCCHHHHCEEECCCCCEEEEEECCCCEECCCCCCCHHHHEEECE
VFLRKTNPRTRASLAYVESITAPDDETVVFQLKQPFGPFIRMFEVGTMPMVPKHIYEGTD
EEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCHHHHHCCCC
FLTNPANNTPIGTGPFKFGEWVKGSYIKLVKNKDYYEKGKPHLDEIYWHIIPDAAARAVA
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHCCCCCHHHEEEEECCCHHHHEEE
FETGKVDILPGGSVENFDIPRLAKTKNACITDKGWEFFAPQSWLWFNHRGGLTADPRFRK
EECCEEEEECCCCCCCCCCCCHHCCCCCEECCCCCEEECCCCEEEEECCCCCCCCHHHHH
AVSYAIDRNFALDVLWNGMGAVSDGPFAQSTSFRDDKAVVKYPYDPAKAKALLKEMGYKG
HHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCC
EPVRFTPMPYGETWLRWAEAIKQNLEDVGIKVEMVNTDVAGWNKKLGDWDFDISITFLYQ
CCEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEEE
YGDPALGVSRAYISSNIAKGSPFNNVGGYSNPEVDKLFAEGAVGFPDSERQKAYSAVQKK
ECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LTEDAVYDWLIDLKFPTLTHCNVKNLITTGIGVNDGFKDAWIE
HHHHHHHHEEEEECCCCCCCCCHHHHHEECCCCCCCCHHHCCC
>Mature Secondary Structure
MKAKLLGTLAASVALSVLAAGGAFAQQGTPKEGGRANVVIQPEPPGLMVGILQNAPTQMV
CCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCEEEEEECCCCCCEEEEEECCCCHHHH
AGNIYEGLLRYDEKLQPKPQLAKSWTISEDGKTYTFKLQPGVKWHDGKPFTAADVVFSAD
HHHHHHHHHHHHHHCCCCCHHHHCEEECCCCCEEEEEECCCCEECCCCCCCHHHHEEECE
VFLRKTNPRTRASLAYVESITAPDDETVVFQLKQPFGPFIRMFEVGTMPMVPKHIYEGTD
EEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCHHHHHCCCC
FLTNPANNTPIGTGPFKFGEWVKGSYIKLVKNKDYYEKGKPHLDEIYWHIIPDAAARAVA
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHCCCCCHHHEEEEECCCHHHHEEE
FETGKVDILPGGSVENFDIPRLAKTKNACITDKGWEFFAPQSWLWFNHRGGLTADPRFRK
EECCEEEEECCCCCCCCCCCCHHCCCCCEECCCCCEEECCCCEEEEECCCCCCCCHHHHH
AVSYAIDRNFALDVLWNGMGAVSDGPFAQSTSFRDDKAVVKYPYDPAKAKALLKEMGYKG
HHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCC
EPVRFTPMPYGETWLRWAEAIKQNLEDVGIKVEMVNTDVAGWNKKLGDWDFDISITFLYQ
CCEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEEE
YGDPALGVSRAYISSNIAKGSPFNNVGGYSNPEVDKLFAEGAVGFPDSERQKAYSAVQKK
ECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LTEDAVYDWLIDLKFPTLTHCNVKNLITTGIGVNDGFKDAWIE
HHHHHHHHEEEEECCCCCCCCCHHHHHEECCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]