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The map label for this gene is nac [H]

Identifier: 158423468

GI number: 158423468

Start: 2108959

End: 2109897

Strand: Direct

Name: nac [H]

Synonym: AZC_1844

Alternate gene names: 158423468

Gene position: 2108959-2109897 (Clockwise)

Preceding gene: 158423467

Following gene: 158423470

Centisome position: 39.27

GC content: 70.5

Gene sequence:

>939_bases
TTGGACCTGCGCCAGCTTCGTTACTTTCTCGGCATCGTGGAGGCGGGCTCCTTCTCCAAGGCCGCGCAGCGTCTGCATGT
GGCCCAGCCGGCGCTGAGCCAGCATGTGCGCAACATGGAACTGGACCTCGGCGTCGAGCTGCTGTTCCGCACGCCGCAGG
GCGTCACCGTGACCGAGGCGGGCGAGACGCTGCTGCGCCATGCCCGCGCCGTGCTCGCCCAAGTGGCGGTGGCCCGCGAG
GAGGTGATCGCCCGGCAGGCGGAGCCGGAAGGCGAGGTGCGCTTCGGCCTGCCGGGCACCATTTCCGAGCGCATCTGCGT
GCCGCTGATTCTGGAGGCCCGCCGCCGCTTTCCGAAGGTGAAGCTGCGGATCGCCGAGGCCATGAGCGGCTTCGTGCTGG
ACTGGCTGCGCGAGGGCAAGATCGACCTCGGCGTGCTCTATCGCACGGTCTCGGATCGCAGGCTGGGCGCTCGTCTCGTG
CTGCGCGAGGATCTGTGCCTGCTCGGCCCGGTAGCGCCCCTCGCTCCCGAGCCACGCCTGAGCAAGGGCCATGTGTCGCT
GGCCGCCGTGGGCGCGCTGCCGCTCATCCTGCCGAGCCCCGGCCACGGCCTGCGCGATCTTCTGGATGAGAGCGCGGCGG
CGGCTGGCGCCGAACTCTCCACCGTCACCGATCTCGACACCTACGGCCAGATCAAGCTGCTGGTGGAGGCGGGGGTCGGC
TATTCCATCCTGCCGGTGGCGGCCGTGCGCCGCGAGGTGGACGAGGGACGGTTGCGCACCTGGCCCATCGGCACCCCCGC
GCTCACCCGCGACCTGCATCTGGTGCACCCCGCCGATCGTCCGCTGGGCAATGCGGTGCGGGCGGTGGAGCAACTGGCGC
TCACCATCCTTCAGCAGCAGGTGCGCGACGGCACATGGCCGGCGACGCTAGCCGATTAA

Upstream 100 bases:

>100_bases
AGCCCCACCGCGCCCATCTGCTGCGTCGGCCGTCATAGGTTTGCGCGCCGTTCCGGCGCGGGGGTAGACTGAAAATCTAT
CCCCCGCCGGAGCCTGATCC

Downstream 100 bases:

>100_bases
GGCAGGTGACAGCCTGCGGCTCAGGACTTCTCGCGTACGTAGCGGCCGGGGGCATCCTCGATGGGATTGAACGCGCTGCC
GCCTGGCTCGCGGGCAGGGA

Product: Nac protein

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MDLRQLRYFLGIVEAGSFSKAAQRLHVAQPALSQHVRNMELDLGVELLFRTPQGVTVTEAGETLLRHARAVLAQVAVARE
EVIARQAEPEGEVRFGLPGTISERICVPLILEARRRFPKVKLRIAEAMSGFVLDWLREGKIDLGVLYRTVSDRRLGARLV
LREDLCLLGPVAPLAPEPRLSKGHVSLAAVGALPLILPSPGHGLRDLLDESAAAAGAELSTVTDLDTYGQIKLLVEAGVG
YSILPVAAVRREVDEGRLRTWPIGTPALTRDLHLVHPADRPLGNAVRAVEQLALTILQQQVRDGTWPATLAD

Sequences:

>Translated_312_residues
MDLRQLRYFLGIVEAGSFSKAAQRLHVAQPALSQHVRNMELDLGVELLFRTPQGVTVTEAGETLLRHARAVLAQVAVARE
EVIARQAEPEGEVRFGLPGTISERICVPLILEARRRFPKVKLRIAEAMSGFVLDWLREGKIDLGVLYRTVSDRRLGARLV
LREDLCLLGPVAPLAPEPRLSKGHVSLAAVGALPLILPSPGHGLRDLLDESAAAAGAELSTVTDLDTYGQIKLLVEAGVG
YSILPVAAVRREVDEGRLRTWPIGTPALTRDLHLVHPADRPLGNAVRAVEQLALTILQQQVRDGTWPATLAD
>Mature_312_residues
MDLRQLRYFLGIVEAGSFSKAAQRLHVAQPALSQHVRNMELDLGVELLFRTPQGVTVTEAGETLLRHARAVLAQVAVARE
EVIARQAEPEGEVRFGLPGTISERICVPLILEARRRFPKVKLRIAEAMSGFVLDWLREGKIDLGVLYRTVSDRRLGARLV
LREDLCLLGPVAPLAPEPRLSKGHVSLAAVGALPLILPSPGHGLRDLLDESAAAAGAELSTVTDLDTYGQIKLLVEAGVG
YSILPVAAVRREVDEGRLRTWPIGTPALTRDLHLVHPADRPLGNAVRAVEQLALTILQQQVRDGTWPATLAD

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=306, Percent_Identity=30.718954248366, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790399, Length=297, Percent_Identity=30.976430976431, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787806, Length=264, Percent_Identity=28.7878787878788, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1787879, Length=295, Percent_Identity=32.2033898305085, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788748, Length=288, Percent_Identity=28.4722222222222, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787601, Length=219, Percent_Identity=28.310502283105, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI157672245, Length=245, Percent_Identity=28.1632653061224, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082132, Length=296, Percent_Identity=27.027027027027, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788481, Length=287, Percent_Identity=27.1777003484321, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786401, Length=154, Percent_Identity=34.4155844155844, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789506, Length=244, Percent_Identity=25, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788887, Length=187, Percent_Identity=28.8770053475936, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI2367136, Length=284, Percent_Identity=27.112676056338, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI145693105, Length=244, Percent_Identity=25.8196721311475, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787530, Length=299, Percent_Identity=27.4247491638796, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787589, Length=303, Percent_Identity=30.3630363036304, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789639, Length=122, Percent_Identity=28.6885245901639, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081904, Length=267, Percent_Identity=25.4681647940075, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790208, Length=145, Percent_Identity=28.9655172413793, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082024, Length=251, Percent_Identity=27.8884462151394, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789204, Length=305, Percent_Identity=23.9344262295082, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33956; Mature: 33956

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRQLRYFLGIVEAGSFSKAAQRLHVAQPALSQHVRNMELDLGVELLFRTPQGVTVTEA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCEEEEEECCCCCEEEHH
GETLLRHARAVLAQVAVAREEVIARQAEPEGEVRFGLPGTISERICVPLILEARRRFPKV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHH
KLRIAEAMSGFVLDWLREGKIDLGVLYRTVSDRRLGARLVLREDLCLLGPVAPLAPEPRL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHCHHHHCCCCCCCCCCCC
SKGHVSLAAVGALPLILPSPGHGLRDLLDESAAAAGAELSTVTDLDTYGQIKLLVEAGVG
CCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEEEECCCC
YSILPVAAVRREVDEGRLRTWPIGTPALTRDLHLVHPADRPLGNAVRAVEQLALTILQQQ
CHHHHHHHHHHHHHCCCEEECCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
VRDGTWPATLAD
HHCCCCCCCCCC
>Mature Secondary Structure
MDLRQLRYFLGIVEAGSFSKAAQRLHVAQPALSQHVRNMELDLGVELLFRTPQGVTVTEA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCEEEEEECCCCCEEEHH
GETLLRHARAVLAQVAVAREEVIARQAEPEGEVRFGLPGTISERICVPLILEARRRFPKV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHH
KLRIAEAMSGFVLDWLREGKIDLGVLYRTVSDRRLGARLVLREDLCLLGPVAPLAPEPRL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHCHHHHCCCCCCCCCCCC
SKGHVSLAAVGALPLILPSPGHGLRDLLDESAAAAGAELSTVTDLDTYGQIKLLVEAGVG
CCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEEEECCCC
YSILPVAAVRREVDEGRLRTWPIGTPALTRDLHLVHPADRPLGNAVRAVEQLALTILQQQ
CHHHHHHHHHHHHHCCCEEECCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
VRDGTWPATLAD
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]