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The map label for this gene is thiD [H]

Identifier: 158423450

GI number: 158423450

Start: 2089374

End: 2090198

Strand: Direct

Name: thiD [H]

Synonym: AZC_1826

Alternate gene names: 158423450

Gene position: 2089374-2090198 (Clockwise)

Preceding gene: 158423447

Following gene: 158423451

Centisome position: 38.91

GC content: 71.39

Gene sequence:

>825_bases
ATGAGCCAGAAGTCTCCCCCCATCGCCGTGACCATCGCCGGCTCGGATTCCAGCGGCGGCGCCGGCATCCAGGCGGACCT
CAAGACCTTCTCCGCCCTCGGCGTCTATGGGGCGAGCGTCATCACCGCGCTGACCGCGCAGAATACGCTCGGTGTCACCG
CCATCCACGACGCGCCGCCCGATTTCATCACCGCCCAGATGGACGCCGTCTTCTCCGACCTGAAGGTCGCGGCGGTGAAG
ATCGGCATGCTCTCGCGCCCCGCCACCATCGCGGCGGTGGCGGACGCGCTTGAACGGCTCGGGGCGCGCAACATCGTGCT
CGATCCGGTGATGGTCGCCGCCTCCGGTGCCCGCCTGCTGGAAGACGCGGCCATCGCCACCCTCAAGGCGCGCCTGCTGC
CGCTCGCCGACGTGGTGACCCCCAATCTGCCGGAAGCGGCCGCTCTGCTGGACGTGCCGGTCGCAGCGGACGAGGCCGCC
ATGCGGGCGCAGGGCGAGGCTCTGCTGGCGCTGGGCGCCAAGGCGGTGCTCATGAAGGGCGGCCATGGCGAGGGCGCGGA
AAGCGTCGATCTTCTGGTGACGCGCGACGCCGTTCTGCGCCTGCCGGCGCCGCGCCACGCCACGCGCAACACCCATGGCA
CCGGCTGCACCCTCTCCTCGGCCATTGCCTCGGGCCTCGCGCGCGGGCTGACGCTCGCCGAGGCCGTGCGGGCGGCCAAG
ACCTATATCACCGGTGCCATTGCCGCCGCCGATCAACTGGAGGTGGGGCAGGGGCATGGTCCCGTCCACCACTTCCATGC
CCTGTGGGGGCAGGCGGGGCTCTGA

Upstream 100 bases:

>100_bases
ACGCCAACCCGCGAACCTGATCCGGATCATGCCGGCGGAGGGAACCGAGACTGCGGCGCGCTTGCGTCCGCGCTCCCCGT
CCGTGGAAGCGAGGCGCCAG

Downstream 100 bases:

>100_bases
GGGCCGCTTTTGCTTGCTCCGTGCCGCTCCTTGCCGCTATCGTCCGAGGAAACGGACAAGTGTTCGGTAAAAGGGAGCGT
CATGGCGCAGCGCGAGAAAC

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSQKSPPIAVTIAGSDSSGGAGIQADLKTFSALGVYGASVITALTAQNTLGVTAIHDAPPDFITAQMDAVFSDLKVAAVK
IGMLSRPATIAAVADALERLGARNIVLDPVMVAASGARLLEDAAIATLKARLLPLADVVTPNLPEAAALLDVPVAADEAA
MRAQGEALLALGAKAVLMKGGHGEGAESVDLLVTRDAVLRLPAPRHATRNTHGTGCTLSSAIASGLARGLTLAEAVRAAK
TYITGAIAAADQLEVGQGHGPVHHFHALWGQAGL

Sequences:

>Translated_274_residues
MSQKSPPIAVTIAGSDSSGGAGIQADLKTFSALGVYGASVITALTAQNTLGVTAIHDAPPDFITAQMDAVFSDLKVAAVK
IGMLSRPATIAAVADALERLGARNIVLDPVMVAASGARLLEDAAIATLKARLLPLADVVTPNLPEAAALLDVPVAADEAA
MRAQGEALLALGAKAVLMKGGHGEGAESVDLLVTRDAVLRLPAPRHATRNTHGTGCTLSSAIASGLARGLTLAEAVRAAK
TYITGAIAAADQLEVGQGHGPVHHFHALWGQAGL
>Mature_273_residues
SQKSPPIAVTIAGSDSSGGAGIQADLKTFSALGVYGASVITALTAQNTLGVTAIHDAPPDFITAQMDAVFSDLKVAAVKI
GMLSRPATIAAVADALERLGARNIVLDPVMVAASGARLLEDAAIATLKARLLPLADVVTPNLPEAAALLDVPVAADEAAM
RAQGEALLALGAKAVLMKGGHGEGAESVDLLVTRDAVLRLPAPRHATRNTHGTGCTLSSAIASGLARGLTLAEAVRAAKT
YITGAIAAADQLEVGQGHGPVHHFHALWGQAGL

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=261, Percent_Identity=57.8544061302682, Blast_Score=301, Evalue=4e-83,
Organism=Saccharomyces cerevisiae, GI6325378, Length=274, Percent_Identity=33.2116788321168, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324997, Length=280, Percent_Identity=34.2857142857143, Blast_Score=132, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6324517, Length=277, Percent_Identity=34.2960288808664, Blast_Score=129, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 27599; Mature: 27468

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKSPPIAVTIAGSDSSGGAGIQADLKTFSALGVYGASVITALTAQNTLGVTAIHDAPP
CCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
DFITAQMDAVFSDLKVAAVKIGMLSRPATIAAVADALERLGARNIVLDPVMVAASGARLL
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECHHHHHHCCHHHH
EDAAIATLKARLLPLADVVTPNLPEAAALLDVPVAADEAAMRAQGEALLALGAKAVLMKG
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCCHHHHHHCCCEEEEECCEEEEEEC
GHGEGAESVDLLVTRDAVLRLPAPRHATRNTHGTGCTLSSAIASGLARGLTLAEAVRAAK
CCCCCCCCEEEEEECCHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TYITGAIAAADQLEVGQGHGPVHHFHALWGQAGL
HHHHHHHHHHCHHCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
SQKSPPIAVTIAGSDSSGGAGIQADLKTFSALGVYGASVITALTAQNTLGVTAIHDAPP
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
DFITAQMDAVFSDLKVAAVKIGMLSRPATIAAVADALERLGARNIVLDPVMVAASGARLL
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECHHHHHHCCHHHH
EDAAIATLKARLLPLADVVTPNLPEAAALLDVPVAADEAAMRAQGEALLALGAKAVLMKG
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCCHHHHHHCCCEEEEECCEEEEEEC
GHGEGAESVDLLVTRDAVLRLPAPRHATRNTHGTGCTLSSAIASGLARGLTLAEAVRAAK
CCCCCCCCEEEEEECCHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TYITGAIAAADQLEVGQGHGPVHHFHALWGQAGL
HHHHHHHHHHCHHCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]