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The map label for this gene is cysS

Identifier: 158423408

GI number: 158423408

Start: 2041525

End: 2042913

Strand: Direct

Name: cysS

Synonym: AZC_1784

Alternate gene names: 158423408

Gene position: 2041525-2042913 (Clockwise)

Preceding gene: 158423406

Following gene: 158423409

Centisome position: 38.02

GC content: 66.31

Gene sequence:

>1389_bases
ATGGAGCTGCGGCTCTACAACACCCTGACGCGGTCGAAGGATGCCTTCCGCCCGCTCGATCCCAATCTCGTGCGCCTCTA
TGTGTGCGGCCCCACGGTCTATGACCATGCCCACATCGGCAACGCCCGTCCGGTCATCGTCTTCGACGTGCTGTTCCGCC
TGCTGCGGCGGCTCTACGGCGCCGACCATGTGCGCTATGTGCGCAACATCACGGACGTGGACGACAAGATCAACGCCCGC
GCGGCCGAGCGCAGCATCTCCATCCGTGAGCTGACGGAAGAGACCTATGCCTGGTTCCGGGACGACACGGCGGCCCTCGG
CTGCCTGCGGCCGGACGTGGAGCCCCGCGCCACCGAGCACATCCTTGAGATGCAGGCGCTGATCGAGCGGCTGGTGGCGA
GCGGCCATGCCTATGTGGCCGAGGAGCACGTGCTCTTCCATGTGCCGTCCATGCCGGAATACGGCAAGCTCTCCCGCCGC
CCGCTGGACGAGATGCTGAATGGCGCCCGCGTGGATGTGGCCCCCTTCAAGCGCGACCCGATGGATTTCGTGCTATGGAA
GCCCAGCACCGAAGGCCAGCCGGGCTGGCCCTCGCCCTGCGGCATCGCGACGCCCGGCCGTCCCGGCTGGCATATCGAGT
GCTCGGCCATGTCCTGGCGGCATCTGGGCGAGACCTTCGATATCCATGGCGGCGGCATCGACCTCGTGTTCCCGCACCAT
GAAAACGAGATCGCCCAGTCGCGCTGCGCCTTCGACAGCGGGTCCATGGCGCAGGTGTGGATGCACAACGGCTTCCTGCT
GGTGGAAGGCGAGAAGATGTCGAAGTCGCTCGGCAACTTCGTCACCATCCGCGAACTTCTGACTGACTGGCCGGGCGAAG
TGCTGCGCCTCGCCATGCTCTCCACCCATTATCGCCAGCCCATCAACTGGACCCGGCAGGGCATCGGTTTCGCCGCCAAG
ACGCTCGACAAGTGGTACCGCGTCATCGGCGACGTGGAAGCGGAGACGGGGGAGGGCAATCCGTTCGAGAGCGAGATCGC
CGACCGGCTGGGCGATGATCTCAACTCGCCCTCGGCCATTACCCACCTGCACCATCTGGCGGACGTGGCCGAGCATGAGG
ACGCCTCCTCAGCGCTGAAGCGGCGCTTCAAGAGCGCGGCCAATCTCATGGGCCTCCTGCACGAGACGGAGACCGAGTGG
CGCGCCCGCCAGCGGGCTGCCGTCTCCGTGGACCCGGATGCCATCGCCGCGCTGATCGCCGATCGCAACGCCGCCCGCAA
GGCGCGCGACTTCGCCACCGCCGATCATATCCGCGAGCAACTGGCCTCGCAGGGCATCGTGCTGATGGACAATAAGGACG
GAACCACCTCCTGGGAGCTGACGCGATGA

Upstream 100 bases:

>100_bases
CCTGCGGGACGGGAGCGTGTGTTTTCATAATGCCGTGACAGCGGCACGTCTGCCCGCCGGCCCCGTGCGCCGGATGCGGC
GCCAGAGGGAGAGCGGACCT

Downstream 100 bases:

>100_bases
GCACCACGGCCCTGCGCCCCGCCTTGCCCGGCGACACCTCCATGCTCGCCTCCATCTTCCGCTCGTCCATCTTCGAGCTG
ACGGAGGAGGATTATGACGA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MELRLYNTLTRSKDAFRPLDPNLVRLYVCGPTVYDHAHIGNARPVIVFDVLFRLLRRLYGADHVRYVRNITDVDDKINAR
AAERSISIRELTEETYAWFRDDTAALGCLRPDVEPRATEHILEMQALIERLVASGHAYVAEEHVLFHVPSMPEYGKLSRR
PLDEMLNGARVDVAPFKRDPMDFVLWKPSTEGQPGWPSPCGIATPGRPGWHIECSAMSWRHLGETFDIHGGGIDLVFPHH
ENEIAQSRCAFDSGSMAQVWMHNGFLLVEGEKMSKSLGNFVTIRELLTDWPGEVLRLAMLSTHYRQPINWTRQGIGFAAK
TLDKWYRVIGDVEAETGEGNPFESEIADRLGDDLNSPSAITHLHHLADVAEHEDASSALKRRFKSAANLMGLLHETETEW
RARQRAAVSVDPDAIAALIADRNAARKARDFATADHIREQLASQGIVLMDNKDGTTSWELTR

Sequences:

>Translated_462_residues
MELRLYNTLTRSKDAFRPLDPNLVRLYVCGPTVYDHAHIGNARPVIVFDVLFRLLRRLYGADHVRYVRNITDVDDKINAR
AAERSISIRELTEETYAWFRDDTAALGCLRPDVEPRATEHILEMQALIERLVASGHAYVAEEHVLFHVPSMPEYGKLSRR
PLDEMLNGARVDVAPFKRDPMDFVLWKPSTEGQPGWPSPCGIATPGRPGWHIECSAMSWRHLGETFDIHGGGIDLVFPHH
ENEIAQSRCAFDSGSMAQVWMHNGFLLVEGEKMSKSLGNFVTIRELLTDWPGEVLRLAMLSTHYRQPINWTRQGIGFAAK
TLDKWYRVIGDVEAETGEGNPFESEIADRLGDDLNSPSAITHLHHLADVAEHEDASSALKRRFKSAANLMGLLHETETEW
RARQRAAVSVDPDAIAALIADRNAARKARDFATADHIREQLASQGIVLMDNKDGTTSWELTR
>Mature_462_residues
MELRLYNTLTRSKDAFRPLDPNLVRLYVCGPTVYDHAHIGNARPVIVFDVLFRLLRRLYGADHVRYVRNITDVDDKINAR
AAERSISIRELTEETYAWFRDDTAALGCLRPDVEPRATEHILEMQALIERLVASGHAYVAEEHVLFHVPSMPEYGKLSRR
PLDEMLNGARVDVAPFKRDPMDFVLWKPSTEGQPGWPSPCGIATPGRPGWHIECSAMSWRHLGETFDIHGGGIDLVFPHH
ENEIAQSRCAFDSGSMAQVWMHNGFLLVEGEKMSKSLGNFVTIRELLTDWPGEVLRLAMLSTHYRQPINWTRQGIGFAAK
TLDKWYRVIGDVEAETGEGNPFESEIADRLGDDLNSPSAITHLHHLADVAEHEDASSALKRRFKSAANLMGLLHETETEW
RARQRAAVSVDPDAIAALIADRNAARKARDFATADHIREQLASQGIVLMDNKDGTTSWELTR

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=313, Percent_Identity=41.5335463258786, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI10835051, Length=250, Percent_Identity=38, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI62240992, Length=250, Percent_Identity=38, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI21269877, Length=250, Percent_Identity=38, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI303304987, Length=250, Percent_Identity=38, Blast_Score=169, Evalue=4e-42,
Organism=Escherichia coli, GI1786737, Length=462, Percent_Identity=46.5367965367965, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17509795, Length=232, Percent_Identity=38.7931034482759, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17509797, Length=232, Percent_Identity=38.7931034482759, Blast_Score=157, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324082, Length=229, Percent_Identity=37.117903930131, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI19922218, Length=380, Percent_Identity=36.0526315789474, Blast_Score=218, Evalue=8e-57,
Organism=Drosophila melanogaster, GI19922364, Length=250, Percent_Identity=38.4, Blast_Score=169, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_AZOC5 (A8I4X6)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001524700.1
- ProteinModelPortal:   A8I4X6
- SMR:   A8I4X6
- GeneID:   5689325
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_1784
- HOGENOM:   HBG327651
- OMA:   INARAAC
- ProtClustDB:   PRK00260
- BioCyc:   ACAU438753:AZC_1784-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 52099; Mature: 52099

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 275-275

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRLYNTLTRSKDAFRPLDPNLVRLYVCGPTVYDHAHIGNARPVIVFDVLFRLLRRLYG
CCEEEHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
ADHVRYVRNITDVDDKINARAAERSISIRELTEETYAWFRDDTAALGCLRPDVEPRATEH
CHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHH
ILEMQALIERLVASGHAYVAEEHVLFHVPSMPEYGKLSRRPLDEMLNGARVDVAPFKRDP
HHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCC
MDFVLWKPSTEGQPGWPSPCGIATPGRPGWHIECSAMSWRHLGETFDIHGGGIDLVFPHH
CCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHCCCEEEECCCCEEEEECCC
ENEIAQSRCAFDSGSMAQVWMHNGFLLVEGEKMSKSLGNFVTIRELLTDWPGEVLRLAML
CCHHHHHHHCCCCCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
STHYRQPINWTRQGIGFAAKTLDKWYRVIGDVEAETGEGNPFESEIADRLGDDLNSPSAI
HHHHCCCCCHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHH
THLHHLADVAEHEDASSALKRRFKSAANLMGLLHETETEWRARQRAAVSVDPDAIAALIA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHH
DRNAARKARDFATADHIREQLASQGIVLMDNKDGTTSWELTR
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEECC
>Mature Secondary Structure
MELRLYNTLTRSKDAFRPLDPNLVRLYVCGPTVYDHAHIGNARPVIVFDVLFRLLRRLYG
CCEEEHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
ADHVRYVRNITDVDDKINARAAERSISIRELTEETYAWFRDDTAALGCLRPDVEPRATEH
CHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHH
ILEMQALIERLVASGHAYVAEEHVLFHVPSMPEYGKLSRRPLDEMLNGARVDVAPFKRDP
HHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCC
MDFVLWKPSTEGQPGWPSPCGIATPGRPGWHIECSAMSWRHLGETFDIHGGGIDLVFPHH
CCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHCCCEEEECCCCEEEEECCC
ENEIAQSRCAFDSGSMAQVWMHNGFLLVEGEKMSKSLGNFVTIRELLTDWPGEVLRLAML
CCHHHHHHHCCCCCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
STHYRQPINWTRQGIGFAAKTLDKWYRVIGDVEAETGEGNPFESEIADRLGDDLNSPSAI
HHHHCCCCCHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHH
THLHHLADVAEHEDASSALKRRFKSAANLMGLLHETETEWRARQRAAVSVDPDAIAALIA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHH
DRNAARKARDFATADHIREQLASQGIVLMDNKDGTTSWELTR
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA