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The map label for this gene is lipA

Identifier: 158423371

GI number: 158423371

Start: 1998017

End: 1998970

Strand: Direct

Name: lipA

Synonym: AZC_1747

Alternate gene names: 158423371

Gene position: 1998017-1998970 (Clockwise)

Preceding gene: 158423370

Following gene: 158423372

Centisome position: 37.21

GC content: 66.04

Gene sequence:

>954_bases
ATGGTCACGGTCGTCAACACGCTGAACCGGCCCCGTCATCCGGAAAAGCAGAACCGTCCGGAAACGGAAGTCCTGCGCAA
GCCGGACTGGATCCGCGTGAAGGCGCCGGGCTCCGCCGGCTGGTCCAACACGGCCGGCATCGTGCGCGCCAATGGCCTGC
ACACGGTGTGCGAGGAAGCCGGCTGCCCGAACATCGGTGAGTGCTGGGAGAAGAAGCACGCCACCTTCATGATCATGGGC
GACACCTGCACGCGCGCCTGCTCTTTCTGCAACGTGCGCACCGGCATGCCCAAGGCGCTCGATCTCGACGAGCCGCAGAA
GGTGGGCGAGGCGGTGGCCAAGCTCGGCCTTTCCCACGTGGTCATCACCTCGGTGGACCGCGACGACCTCACTGACGGCG
GGGCCGAGCATTTCGCCCGCACGATTGCGTCCATCCGCAAGCTCAGCCCCGGCACCACCATCGAGATCCTGACCCCGGAC
TTCCTGCGCAAGCCGGGGGCCATAGAGGTGGTGGTCGCGGCCCGCCCCGACGTCTTCAACCACAATCTGGAGACGGTGCC
GGGCAAGTATCTGACGGTGCGACCCGGCGCGCGCTACTTCCATTCGCTCCGCCTGCTCCAGCAGGTGAAGGAACTCGACC
CGTCCATCTTCACCAAGTCCGGCATCATGGTCGGGCTCGGCGAGGAGCGGAACGAGGTGCTCCAGCTCATGGATGACCTG
CGGGCGGCGGAAGTCGACTTCATGACCATCGGCCAGTATCTCCAGCCCACCCGCAAGCACCACAAGGTGGAGCGCTTCGT
GACGCCGGACGAGTTCAAGGCCTATGAGACGGTGGCCTATGCCAAGGGCTTCCTGATGGTCTCGTCCAGCCCGCTCACCC
GCTCGTCGCACCATGCGGGCGACGATTTCGCCAAGCTCAGGGCCGCCCGCGAGGCGAAGCTCGGCCGGATCTGA

Upstream 100 bases:

>100_bases
TCGTTTGCGGCGCGGCGCCTCGACGCTGGCGGCGGCCGCAGCTAAGACATATGTGAATGACGGCCCGGCTCGGGGCCGGC
TCGAAACAGATGCGGTCCCC

Downstream 100 bases:

>100_bases
TCGCGTGCCCTCCTTCTCGACCAAGAGGGTGGTGCGCCACTCGCCGCTCAACATGTTCGATCTCGTGGCGGATGTGGAGC
AGTATCCGGAGTTCGTGCCT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MVTVVNTLNRPRHPEKQNRPETEVLRKPDWIRVKAPGSAGWSNTAGIVRANGLHTVCEEAGCPNIGECWEKKHATFMIMG
DTCTRACSFCNVRTGMPKALDLDEPQKVGEAVAKLGLSHVVITSVDRDDLTDGGAEHFARTIASIRKLSPGTTIEILTPD
FLRKPGAIEVVVAARPDVFNHNLETVPGKYLTVRPGARYFHSLRLLQQVKELDPSIFTKSGIMVGLGEERNEVLQLMDDL
RAAEVDFMTIGQYLQPTRKHHKVERFVTPDEFKAYETVAYAKGFLMVSSSPLTRSSHHAGDDFAKLRAAREAKLGRI

Sequences:

>Translated_317_residues
MVTVVNTLNRPRHPEKQNRPETEVLRKPDWIRVKAPGSAGWSNTAGIVRANGLHTVCEEAGCPNIGECWEKKHATFMIMG
DTCTRACSFCNVRTGMPKALDLDEPQKVGEAVAKLGLSHVVITSVDRDDLTDGGAEHFARTIASIRKLSPGTTIEILTPD
FLRKPGAIEVVVAARPDVFNHNLETVPGKYLTVRPGARYFHSLRLLQQVKELDPSIFTKSGIMVGLGEERNEVLQLMDDL
RAAEVDFMTIGQYLQPTRKHHKVERFVTPDEFKAYETVAYAKGFLMVSSSPLTRSSHHAGDDFAKLRAAREAKLGRI
>Mature_317_residues
MVTVVNTLNRPRHPEKQNRPETEVLRKPDWIRVKAPGSAGWSNTAGIVRANGLHTVCEEAGCPNIGECWEKKHATFMIMG
DTCTRACSFCNVRTGMPKALDLDEPQKVGEAVAKLGLSHVVITSVDRDDLTDGGAEHFARTIASIRKLSPGTTIEILTPD
FLRKPGAIEVVVAARPDVFNHNLETVPGKYLTVRPGARYFHSLRLLQQVKELDPSIFTKSGIMVGLGEERNEVLQLMDDL
RAAEVDFMTIGQYLQPTRKHHKVERFVTPDEFKAYETVAYAKGFLMVSSSPLTRSSHHAGDDFAKLRAAREAKLGRI

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=303, Percent_Identity=46.8646864686469, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI37577164, Length=252, Percent_Identity=47.2222222222222, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI1786846, Length=278, Percent_Identity=52.158273381295, Blast_Score=296, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI32564533, Length=258, Percent_Identity=46.1240310077519, Blast_Score=236, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=300, Percent_Identity=43, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI221513272, Length=295, Percent_Identity=43.3898305084746, Blast_Score=250, Evalue=9e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_AZOC5 (A8I4L8)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001524663.1
- ProteinModelPortal:   A8I4L8
- GeneID:   5688143
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_1747
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   ACAU438753:AZC_1747-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35169; Mature: 35169

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTVVNTLNRPRHPEKQNRPETEVLRKPDWIRVKAPGSAGWSNTAGIVRANGLHTVCEEA
CEEEEECCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCCCCCCCCCEEEECCHHHHHHHC
GCPNIGECWEKKHATFMIMGDTCTRACSFCNVRTGMPKALDLDEPQKVGEAVAKLGLSHV
CCCCHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEE
VITSVDRDDLTDGGAEHFARTIASIRKLSPGTTIEILTPDFLRKPGAIEVVVAARPDVFN
EEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEEEEECCCHHC
HNLETVPGKYLTVRPGARYFHSLRLLQQVKELDPSIFTKSGIMVGLGEERNEVLQLMDDL
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHEECCCEEEECCCCHHHHHHHHHHH
RAAEVDFMTIGQYLQPTRKHHKVERFVTPDEFKAYETVAYAKGFLMVSSSPLTRSSHHAG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCC
DDFAKLRAAREAKLGRI
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVTVVNTLNRPRHPEKQNRPETEVLRKPDWIRVKAPGSAGWSNTAGIVRANGLHTVCEEA
CEEEEECCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCCCCCCCCCEEEECCHHHHHHHC
GCPNIGECWEKKHATFMIMGDTCTRACSFCNVRTGMPKALDLDEPQKVGEAVAKLGLSHV
CCCCHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEE
VITSVDRDDLTDGGAEHFARTIASIRKLSPGTTIEILTPDFLRKPGAIEVVVAARPDVFN
EEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEEEEECCCHHC
HNLETVPGKYLTVRPGARYFHSLRLLQQVKELDPSIFTKSGIMVGLGEERNEVLQLMDDL
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHEECCCEEEECCCCHHHHHHHHHHH
RAAEVDFMTIGQYLQPTRKHHKVERFVTPDEFKAYETVAYAKGFLMVSSSPLTRSSHHAG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCC
DDFAKLRAAREAKLGRI
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA