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The map label for this gene is lpdA [H]

Identifier: 158423370

GI number: 158423370

Start: 1996453

End: 1997871

Strand: Direct

Name: lpdA [H]

Synonym: AZC_1746

Alternate gene names: 158423370

Gene position: 1996453-1997871 (Clockwise)

Preceding gene: 158423369

Following gene: 158423371

Centisome position: 37.18

GC content: 65.61

Gene sequence:

>1419_bases
ATGGCTGAGACTTCCTACGATGTGATCGTGATCGGCGGCGGCCCCGGCGGCTATGTGGCCGCCATCCGCGCCGCGCAGCT
CGGATTCAAGACCGCGGTGGTGGAGAAGAAGCACCTCGGCGGCATCTGCCTCAACTGGGGCTGCATCCCCACCAAGGCCC
TGCTGCGCTCCGCCGAAATCTATCACTATATGGAGCACGCCAAGGACTACGGCCTCTCCGCCGAGAAGATCGGCTTCGAC
ATCGGCGCGGTGGTGAAGCGCTCGCGCGGCGTGTCGCAGCAGCTGAACACCGGCGTCGGCTTCCTGATGAAGAAGAACAA
GATCGACGTGATCTGGGGCTCGGCCACCCTGACGGCGCCGGGCAAGATCAAGGTCGAGGCGGCCCCCGATGCCCCCAAGG
GCGCACTCGGCGGCGGCGACTATCTCGGCAAGCACATCATCGTCGCCACCGGTGCCCGCCCGCGCGCGCTGCCCGGCATC
GAGCCCGACAAGAAGCTCATCTGGACCTATTTCGAGGCCATGGTGCCCGACCGCATGCCGAAGTCGCTGCTGGTCATGGG
TTCCGGCGCCATCGGCATCGAGTTCGCCTCCTTCTACAAGACCATGGGCGCCGATGTGACCGTGGTCGAGGTGATGCCGC
AGATCCTCCCCGTGGAGGATGAGGAGATCGCCGCCCTCGCGCGCAAGCGCTTCGAGAAGCAGGGCATGAAGATCCTCTCC
GGCGCCAAGGTGACGGGCGTCACCAAGCAGGCGGACAGCCTCACCGTGCACGTGGAGGATTCCAAGGGCGCCAAGCAGGA
CTTCCAGGTGGAGCGCATGATCTCCGCCGTGGGCGTGGTCGGCAATGTGGACGGCCTCGGCCTCGAGAACCTCGGTGTGA
AGATCGAGCGCGGCATCATCGTCACGGACGGCTACGGCCGCACCAACGTGCCGGGCGTCTATGCCATCGGCGACGTGGCG
GGGGCGCCCATGCTGGCCCACAAGGCCGAGCATGAGGGCGTGATCTGCGTCGAGACCATCAAGGGTCTCCACACCCACCC
AATGGACAAGGCCAAGATCCCCGGCTGCACCTATTGCACGCCGCAGATCGCCTCCGTCGGCCTCACCGAGAAGAAGGCCC
GCGAGCTCGGCCGTGACATCCGCGTCGGCCGCTTCCCCTTCATCGGCAACGGCAAGGCCATTGCGCTGGGCGAGCCGGAG
GGTCTGGTCAAGACCATCTTCGACAAGAAAACCGGCGAGCTGCTGGGCGCCCATCTCATTGGTGCGGAAGTGACCGAGCT
GATCCAGGGCTTCGTCATCGCCATGAACCTTGAGACCACCGAGGAAGAGCTGATCCACGCGGTGTTCCCGCATCCCACGC
TCTCCGAGACCATGCACGAGAGCGTGCTGGACGCCTACGGGCGCGTGATCCACACCTGA

Upstream 100 bases:

>100_bases
GCCTGACCTCAGGCTCCGCTCCGGCCGCGCATGCGGGAGGATCAGCAGGAAGACCCCATCGACCCACCGGCCGCGCGCGG
CGGAAGGGGAGGAGAGACCC

Downstream 100 bases:

>100_bases
TCGGGCGCCAGCGCCCGGGCGGCACTGTCGCGCCCGGCGCATTCCTCGTTTGCGGCGCGGCGCCTCGACGCTGGCGGCGG
CCGCAGCTAAGACATATGTG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAETSYDVIVIGGGPGGYVAAIRAAQLGFKTAVVEKKHLGGICLNWGCIPTKALLRSAEIYHYMEHAKDYGLSAEKIGFD
IGAVVKRSRGVSQQLNTGVGFLMKKNKIDVIWGSATLTAPGKIKVEAAPDAPKGALGGGDYLGKHIIVATGARPRALPGI
EPDKKLIWTYFEAMVPDRMPKSLLVMGSGAIGIEFASFYKTMGADVTVVEVMPQILPVEDEEIAALARKRFEKQGMKILS
GAKVTGVTKQADSLTVHVEDSKGAKQDFQVERMISAVGVVGNVDGLGLENLGVKIERGIIVTDGYGRTNVPGVYAIGDVA
GAPMLAHKAEHEGVICVETIKGLHTHPMDKAKIPGCTYCTPQIASVGLTEKKARELGRDIRVGRFPFIGNGKAIALGEPE
GLVKTIFDKKTGELLGAHLIGAEVTELIQGFVIAMNLETTEEELIHAVFPHPTLSETMHESVLDAYGRVIHT

Sequences:

>Translated_472_residues
MAETSYDVIVIGGGPGGYVAAIRAAQLGFKTAVVEKKHLGGICLNWGCIPTKALLRSAEIYHYMEHAKDYGLSAEKIGFD
IGAVVKRSRGVSQQLNTGVGFLMKKNKIDVIWGSATLTAPGKIKVEAAPDAPKGALGGGDYLGKHIIVATGARPRALPGI
EPDKKLIWTYFEAMVPDRMPKSLLVMGSGAIGIEFASFYKTMGADVTVVEVMPQILPVEDEEIAALARKRFEKQGMKILS
GAKVTGVTKQADSLTVHVEDSKGAKQDFQVERMISAVGVVGNVDGLGLENLGVKIERGIIVTDGYGRTNVPGVYAIGDVA
GAPMLAHKAEHEGVICVETIKGLHTHPMDKAKIPGCTYCTPQIASVGLTEKKARELGRDIRVGRFPFIGNGKAIALGEPE
GLVKTIFDKKTGELLGAHLIGAEVTELIQGFVIAMNLETTEEELIHAVFPHPTLSETMHESVLDAYGRVIHT
>Mature_471_residues
AETSYDVIVIGGGPGGYVAAIRAAQLGFKTAVVEKKHLGGICLNWGCIPTKALLRSAEIYHYMEHAKDYGLSAEKIGFDI
GAVVKRSRGVSQQLNTGVGFLMKKNKIDVIWGSATLTAPGKIKVEAAPDAPKGALGGGDYLGKHIIVATGARPRALPGIE
PDKKLIWTYFEAMVPDRMPKSLLVMGSGAIGIEFASFYKTMGADVTVVEVMPQILPVEDEEIAALARKRFEKQGMKILSG
AKVTGVTKQADSLTVHVEDSKGAKQDFQVERMISAVGVVGNVDGLGLENLGVKIERGIIVTDGYGRTNVPGVYAIGDVAG
APMLAHKAEHEGVICVETIKGLHTHPMDKAKIPGCTYCTPQIASVGLTEKKARELGRDIRVGRFPFIGNGKAIALGEPEG
LVKTIFDKKTGELLGAHLIGAEVTELIQGFVIAMNLETTEEELIHAVFPHPTLSETMHESVLDAYGRVIHT

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=475, Percent_Identity=37.8947368421053, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI50301238, Length=472, Percent_Identity=27.9661016949153, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI22035672, Length=488, Percent_Identity=28.2786885245902, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI33519430, Length=485, Percent_Identity=27.8350515463918, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI33519428, Length=485, Percent_Identity=27.8350515463918, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI33519426, Length=485, Percent_Identity=27.8350515463918, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI148277065, Length=485, Percent_Identity=27.2164948453608, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI291045266, Length=475, Percent_Identity=27.1578947368421, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI148277071, Length=485, Percent_Identity=27.2164948453608, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI291045268, Length=473, Percent_Identity=26.8498942917548, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1786307, Length=458, Percent_Identity=35.1528384279476, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI87082354, Length=472, Percent_Identity=27.7542372881356, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI87081717, Length=461, Percent_Identity=28.8503253796095, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1789915, Length=444, Percent_Identity=29.7297297297297, Blast_Score=159, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI32565766, Length=474, Percent_Identity=37.1308016877637, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17557007, Length=482, Percent_Identity=25.9336099585062, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71983429, Length=445, Percent_Identity=26.0674157303371, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=445, Percent_Identity=26.0674157303371, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=498, Percent_Identity=25.7028112449799, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17559934, Length=187, Percent_Identity=31.0160427807487, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=39.5789473684211, Blast_Score=301, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6325166, Length=472, Percent_Identity=29.4491525423729, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6325240, Length=473, Percent_Identity=27.6955602536998, Blast_Score=170, Evalue=4e-43,
Organism=Drosophila melanogaster, GI21358499, Length=473, Percent_Identity=39.1120507399577, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24640549, Length=474, Percent_Identity=29.3248945147679, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24640553, Length=474, Percent_Identity=29.3248945147679, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24640551, Length=473, Percent_Identity=29.3868921775898, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI17737741, Length=473, Percent_Identity=27.906976744186, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50355; Mature: 50224

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETSYDVIVIGGGPGGYVAAIRAAQLGFKTAVVEKKHLGGICLNWGCIPTKALLRSAEI
CCCCCCCEEEEECCCCCHHHHHHHHHHCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHH
YHYMEHAKDYGLSAEKIGFDIGAVVKRSRGVSQQLNTGVGFLMKKNKIDVIWGSATLTAP
HHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCHHHHCCCEEEEEECCEEEEEECCEEECCC
GKIKVEAAPDAPKGALGGGDYLGKHIIVATGARPRALPGIEPDKKLIWTYFEAMVPDRMP
CEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
KSLLVMGSGAIGIEFASFYKTMGADVTVVEVMPQILPVEDEEIAALARKRFEKQGMKILS
CEEEEEECCCCHHHHHHHHHHHCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEECC
GAKVTGVTKQADSLTVHVEDSKGAKQDFQVERMISAVGVVGNVDGLGLENLGVKIERGII
CCEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHCCCEEEECCEE
VTDGYGRTNVPGVYAIGDVAGAPMLAHKAEHEGVICVETIKGLHTHPMDKAKIPGCTYCT
EECCCCCCCCCCEEEECCCCCCCHHHHCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCC
PQIASVGLTEKKARELGRDIRVGRFPFIGNGKAIALGEPEGLVKTIFDKKTGELLGAHLI
HHHHHCCCCHHHHHHCCCCCCCCCCCEECCCCEEEECCCCHHHHHHHCCHHHHHHHHHHH
GAEVTELIQGFVIAMNLETTEEELIHAVFPHPTLSETMHESVLDAYGRVIHT
HHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AETSYDVIVIGGGPGGYVAAIRAAQLGFKTAVVEKKHLGGICLNWGCIPTKALLRSAEI
CCCCCCEEEEECCCCCHHHHHHHHHHCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHH
YHYMEHAKDYGLSAEKIGFDIGAVVKRSRGVSQQLNTGVGFLMKKNKIDVIWGSATLTAP
HHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCHHHHCCCEEEEEECCEEEEEECCEEECCC
GKIKVEAAPDAPKGALGGGDYLGKHIIVATGARPRALPGIEPDKKLIWTYFEAMVPDRMP
CEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
KSLLVMGSGAIGIEFASFYKTMGADVTVVEVMPQILPVEDEEIAALARKRFEKQGMKILS
CEEEEEECCCCHHHHHHHHHHHCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEECC
GAKVTGVTKQADSLTVHVEDSKGAKQDFQVERMISAVGVVGNVDGLGLENLGVKIERGII
CCEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHCCCEEEECCEE
VTDGYGRTNVPGVYAIGDVAGAPMLAHKAEHEGVICVETIKGLHTHPMDKAKIPGCTYCT
EECCCCCCCCCCEEEECCCCCCCHHHHCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCC
PQIASVGLTEKKARELGRDIRVGRFPFIGNGKAIALGEPEGLVKTIFDKKTGELLGAHLI
HHHHHCCCCHHHHHHCCCCCCCCCCCEECCCCEEEECCCCHHHHHHHCCHHHHHHHHHHH
GAEVTELIQGFVIAMNLETTEEELIHAVFPHPTLSETMHESVLDAYGRVIHT
HHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]