Click here to switch to the map view.

The map label for this gene is glnT [H]

Identifier: 158423123

GI number: 158423123

Start: 1701144

End: 1702496

Strand: Reverse

Name: glnT [H]

Synonym: AZC_1499

Alternate gene names: 158423123

Gene position: 1702496-1701144 (Counterclockwise)

Preceding gene: 158423124

Following gene: 158423120

Centisome position: 31.71

GC content: 64.38

Gene sequence:

>1353_bases
ATGGCCCGTTTGCCGAAGCAGGTGAAGGACGCGGAAGGCCGCGCCTCGGCCGCCAGCGCCAGCCTTGCGACGATCGCGCA
GGAGCGCGGCATCAAGTATTTCCTCATCTCCTACGTGGACCTGTTCGGTGGCCTGCGCGCCAAGCTCGTGCCCGCCAGCG
CCATCGAGGAGATGCAGAAGGCGGGCGCGGGTTTCGCCGGCTTCGCCACCTGGCTCGACATGAGCCCGGCCGATCCGGAT
CTCTTCGCCGTGCCGGACCCGTCCTCCCTCATCCAGCTGCCGTGGAACCCGGAAGTGGGCTGGCTCGCCTCCGATCTCGT
CATGGACGGCAAGATCGTGGAGCAGGCGCCGCGTTCGGTCCTGAAAGCCCAGCTCGCCCGCGCCGAGGCCCTCGGCTATG
AGATGAAGAGCGGCGTCGAGTGCGAATATTTCCTCATCTCCGCCGATGGCTCGGCCATCCATGACGGCTCCGACACCGCC
GCCAAGCCCTGCTACGACCAGTCGGCCCTGATGCGCCAGTATGGCGTCATCAAGGAGATCTGCGACAGCATGCTCCAGCT
CGGCTGGGGCGCCTATCAGAACGACCATGAGGACGCCAACGGCCAGTTCGAGATGAACTGGAATTATGACAACGCCCTCA
TCACGGCGGACCGGCACGTCTTCTTCAAATATATGGTGAAAAGCATCGCCGAGAAGCACGGCCTGCGTGCCACCTTCATG
CCGAAGCCCTTCATCAACCTCACCGGCTCGGGCTGCCACGTGCATGTGTCGCTGTGGAAGAAGGGCGGCAACGCCTTCTC
CGATCCGAAGGGGGAACTGGGCGTCTCGCAGCTCGGCTATCACTTCATCGGCGGCCTCATCCACAATGCGGATGCGCTCT
GCGCCCTCACCAATCCGACGGTGAATTCCTACAAGCGCATCAATGCCCCGCGCACCATTTCCGGCGCCACCTGGGCGCCG
AACACGGTGACCTACACGGGCAACAACCGCACCCATATGATCCGCATTCCGGACGCGGGCCGCTTCGAATTCCGCCTCGC
GGATGGCGCGGCGAACCCCTACCTGTTGCAGGCGGGCGTGCTGGCCTCGGGCCTCGACGGCATCGAGAACGAGCGCGATC
CGGGCAAGCGGCTCGACATCAACATGTACACGGAAGGCCACAAAGTGCGCGGCGCCAAGCGCCTGCCGCTGAACTTGCTG
GACGCCCTGCGCGCGCTCGAAAAGTCCAAGGTGCTGAAGGACTCCCTCGGCACCGCACTGGTGGACGGCTATCTGAAGCT
GAAGACCGAGGAATGGAACGCCTATTCCCGCCACCTCACCCAGTGGGAACGCGAGCACACGCTGGACATCTGA

Upstream 100 bases:

>100_bases
GGCGGCTTCTGATCGCTCACCCCACCCCGCGTCGTCCTGAAGGGCGGCGCGGGCTTTTCAAATAAAGCCTCCCAGACGAA
CCAAATTCAGGGGAACACAC

Downstream 100 bases:

>100_bases
GGGCGAATATCGCCAACCAGACGAGCCCCCGGCATGCCCATGCCGGGGGCTTTTGTTTTGGCTATTCCGGCGCCGGCGAG
GACAGCGGCTTGTCCTTCTC

Product: glutamine synthetase III

Products: NA

Alternate protein names: Glutamate--ammonia ligase; Glutamine synthetase III; GSIII [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MARLPKQVKDAEGRASAASASLATIAQERGIKYFLISYVDLFGGLRAKLVPASAIEEMQKAGAGFAGFATWLDMSPADPD
LFAVPDPSSLIQLPWNPEVGWLASDLVMDGKIVEQAPRSVLKAQLARAEALGYEMKSGVECEYFLISADGSAIHDGSDTA
AKPCYDQSALMRQYGVIKEICDSMLQLGWGAYQNDHEDANGQFEMNWNYDNALITADRHVFFKYMVKSIAEKHGLRATFM
PKPFINLTGSGCHVHVSLWKKGGNAFSDPKGELGVSQLGYHFIGGLIHNADALCALTNPTVNSYKRINAPRTISGATWAP
NTVTYTGNNRTHMIRIPDAGRFEFRLADGAANPYLLQAGVLASGLDGIENERDPGKRLDINMYTEGHKVRGAKRLPLNLL
DALRALEKSKVLKDSLGTALVDGYLKLKTEEWNAYSRHLTQWEREHTLDI

Sequences:

>Translated_450_residues
MARLPKQVKDAEGRASAASASLATIAQERGIKYFLISYVDLFGGLRAKLVPASAIEEMQKAGAGFAGFATWLDMSPADPD
LFAVPDPSSLIQLPWNPEVGWLASDLVMDGKIVEQAPRSVLKAQLARAEALGYEMKSGVECEYFLISADGSAIHDGSDTA
AKPCYDQSALMRQYGVIKEICDSMLQLGWGAYQNDHEDANGQFEMNWNYDNALITADRHVFFKYMVKSIAEKHGLRATFM
PKPFINLTGSGCHVHVSLWKKGGNAFSDPKGELGVSQLGYHFIGGLIHNADALCALTNPTVNSYKRINAPRTISGATWAP
NTVTYTGNNRTHMIRIPDAGRFEFRLADGAANPYLLQAGVLASGLDGIENERDPGKRLDINMYTEGHKVRGAKRLPLNLL
DALRALEKSKVLKDSLGTALVDGYLKLKTEEWNAYSRHLTQWEREHTLDI
>Mature_449_residues
ARLPKQVKDAEGRASAASASLATIAQERGIKYFLISYVDLFGGLRAKLVPASAIEEMQKAGAGFAGFATWLDMSPADPDL
FAVPDPSSLIQLPWNPEVGWLASDLVMDGKIVEQAPRSVLKAQLARAEALGYEMKSGVECEYFLISADGSAIHDGSDTAA
KPCYDQSALMRQYGVIKEICDSMLQLGWGAYQNDHEDANGQFEMNWNYDNALITADRHVFFKYMVKSIAEKHGLRATFMP
KPFINLTGSGCHVHVSLWKKGGNAFSDPKGELGVSQLGYHFIGGLIHNADALCALTNPTVNSYKRINAPRTISGATWAPN
TVTYTGNNRTHMIRIPDAGRFEFRLADGAANPYLLQAGVLASGLDGIENERDPGKRLDINMYTEGHKVRGAKRLPLNLLD
ALRALEKSKVLKDSLGTALVDGYLKLKTEEWNAYSRHLTQWEREHTLDI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family. Type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=423, Percent_Identity=24.113475177305, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1790301, Length=461, Percent_Identity=31.0195227765727, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI87081870, Length=477, Percent_Identity=28.5115303983228, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR017536 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 49440; Mature: 49309

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLPKQVKDAEGRASAASASLATIAQERGIKYFLISYVDLFGGLRAKLVPASAIEEMQK
CCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHEECCHHHHHHHHH
AGAGFAGFATWLDMSPADPDLFAVPDPSSLIQLPWNPEVGWLASDLVMDGKIVEQAPRSV
HCCCHHHHHHHHCCCCCCCCEEECCCCCCEEECCCCCCCCHHHHHHHHCCHHHHHHHHHH
LKAQLARAEALGYEMKSGVECEYFLISADGSAIHDGSDTAAKPCYDQSALMRQYGVIKEI
HHHHHHHHHHHCHHHHCCCCEEEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHH
CDSMLQLGWGAYQNDHEDANGQFEMNWNYDNALITADRHVFFKYMVKSIAEKHGLRATFM
HHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCEEEEC
PKPFINLTGSGCHVHVSLWKKGGNAFSDPKGELGVSQLGYHFIGGLIHNADALCALTNPT
CCCCEEECCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
VNSYKRINAPRTISGATWAPNTVTYTGNNRTHMIRIPDAGRFEFRLADGAANPYLLQAGV
CHHHHHCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCEEEEEECCCCCCEEEECCH
LASGLDGIENERDPGKRLDINMYTEGHKVRGAKRLPLNLLDALRALEKSKVLKDSLGTAL
HHHCCCCCCCCCCCCCEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VDGYLKLKTEEWNAYSRHLTQWEREHTLDI
HCCEEEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ARLPKQVKDAEGRASAASASLATIAQERGIKYFLISYVDLFGGLRAKLVPASAIEEMQK
CCCCCHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHEECCHHHHHHHHH
AGAGFAGFATWLDMSPADPDLFAVPDPSSLIQLPWNPEVGWLASDLVMDGKIVEQAPRSV
HCCCHHHHHHHHCCCCCCCCEEECCCCCCEEECCCCCCCCHHHHHHHHCCHHHHHHHHHH
LKAQLARAEALGYEMKSGVECEYFLISADGSAIHDGSDTAAKPCYDQSALMRQYGVIKEI
HHHHHHHHHHHCHHHHCCCCEEEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHH
CDSMLQLGWGAYQNDHEDANGQFEMNWNYDNALITADRHVFFKYMVKSIAEKHGLRATFM
HHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCEEEEC
PKPFINLTGSGCHVHVSLWKKGGNAFSDPKGELGVSQLGYHFIGGLIHNADALCALTNPT
CCCCEEECCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
VNSYKRINAPRTISGATWAPNTVTYTGNNRTHMIRIPDAGRFEFRLADGAANPYLLQAGV
CHHHHHCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCEEEEEECCCCCCEEEECCH
LASGLDGIENERDPGKRLDINMYTEGHKVRGAKRLPLNLLDALRALEKSKVLKDSLGTAL
HHHCCCCCCCCCCCCCEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VDGYLKLKTEEWNAYSRHLTQWEREHTLDI
HCCEEEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1356885 [H]