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The map label for this gene is alkK [H]

Identifier: 158423100

GI number: 158423100

Start: 1676487

End: 1678112

Strand: Reverse

Name: alkK [H]

Synonym: AZC_1476

Alternate gene names: 158423100

Gene position: 1678112-1676487 (Counterclockwise)

Preceding gene: 158423102

Following gene: 158423099

Centisome position: 31.25

GC content: 66.67

Gene sequence:

>1626_bases
ATGACATCGATCTTCGATCAGCATCTCGACCGCAATCCCGCCAATTACCAGCCGCTGACGCCGCTCGGCTTCCTGGAGCG
GGCGGCGAGCGTCTTCCCCGATCAGGTGGCGGTCATCCACGGGCCGCTGCGCCGCACCTATCGCGAGCTCTATGCCCGCA
CGCGCCGCCTTGCCTCGGCGCTGGCGCAGCGCGGCATCGGGGTGGGCGACACGGTGGCGGTGATGCTCTCCAACACCCCG
CCCATGCTGGAAGCCCACTATGGCGTGCCCATGTGCGGGGCGGTGCTCAACACCTTCAACACCCGGCTCGACGCCGCCAT
CCTCGCCTTCACCATCGACCATGGCGAGGCGAAGGTGCTGATCACCGACCGGGAATTCTCGCCCGTCATCAAGGCGGCGC
TGTCGCTCGCCACCCGCAAACCGCTGGTGATCGATTATGACGACCCGGAATATGCCGGGCCCGGCGAGCGGCTCGGCGCC
ATCGAATATGAGGACTTCATCGCCGCCGGCGATCCGGATTTCGTGTGGGCGCCGCCCGCCGACGAATGGGACGCCATCTC
GCTCAACTACACCTCGGGCACCACGGGCGACCCCAAGGGCGTCGTCTATCACCATCGCGGCGCGCACCTGCTCTCGGTGG
GCAATGTGGTGACCTGCCATATGGGTCCGCACCCGGTCTATCTGTGGACGCTGCCCATGTTCCACTGCAACGGCTGGTGC
TTCCCCTGGTCGGTCTCGGTGGTGGCCGGCACTCACGTGTGCCTGCGGCAGGTCCGCGCCAAGGCCATCTATGAGGCCAT
CGAGGACCACAAGGTCACCCATATGTGCGGCGCCCCCATCGTCATGTCGACGCTGCTGAATGCGCCGGAGACGGACCGCC
GACCCCTGCCCCACACCGTCTCCTTCATCACCGCCGCCGCTCCGCCGCCCGCCGCCGTGCTCGCGGCCATGCAGGAGGCC
GGCTTCAATGTGGTGCATGTCTATGGCCTGACCGAAGTCTATGGCCCGGCGGTGGTGAATGACTGGCATGGCGAATGGGA
CGCCCTGCCCCCTGCCGAGCGCGCGGCGAAGAAGGCCCGGCAAGGCGTGCGCTATGGCGCGCTCGAAGCCCTCGACGTGC
TCGATCCCCTGACCATGGAGCCCGTGCCAGCGGATGGCGAGACCATCGGCGAGGTGATGTTCCGGGGCAATGTGGTGATG
AAGGGCTATGTGAAGAACCCCGCCGCCACGGATGCCGCCTTCGCCGGCGGCTGGTTCCACTCCGGCGACCTCGGGGTGAA
GCATCCCGACGGCTATATCCAGCTCAAGGACCGCTCCAAGGACATCATCATCTCGGGCGGGGAGAACATCTCCTCCATCG
AGGTGGAAGACGCCCTCTACAAGCATCCGGCGGTGAGCGCCGCCGCCGTGGTGGCCCGGCCGGACGAAAAATGGGGCGAG
ACGCCACTCGCCTTCGTGGAGCTGAAGGACGGGGCCGCCGCCTCAGCCGAGGATCTCATCGCCCACTGCCGGGCGCATCT
GGCCGCCTACAAATGCCCGCGAACCATCCTGTTCGAGGAGATCCCCAAGACCTCCACCGGCAAGATCCAGAAATTCAAGC
TCCGCGAACTGGCGAAGGGTCTCTGA

Upstream 100 bases:

>100_bases
TTCTGCGTAAGACCTTGGTCTCAGAACGGCCCGCGTTGCGCCGGCGCGGGCGGCTCGGCTACGGTCACGTCAAACATAAA
GACATATCGGGAAACGCCGC

Downstream 100 bases:

>100_bases
CCTTACGCCATCCTTCCGCTTCGGAAGGACAGTCGCGGCCACTTTTCTTGCGAATTCGTAATCTTGCCGCCATGGTCCTG
CCATGCGGCGCGCGCGTAAG

Product: AMP-binding protein

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MTSIFDQHLDRNPANYQPLTPLGFLERAASVFPDQVAVIHGPLRRTYRELYARTRRLASALAQRGIGVGDTVAVMLSNTP
PMLEAHYGVPMCGAVLNTFNTRLDAAILAFTIDHGEAKVLITDREFSPVIKAALSLATRKPLVIDYDDPEYAGPGERLGA
IEYEDFIAAGDPDFVWAPPADEWDAISLNYTSGTTGDPKGVVYHHRGAHLLSVGNVVTCHMGPHPVYLWTLPMFHCNGWC
FPWSVSVVAGTHVCLRQVRAKAIYEAIEDHKVTHMCGAPIVMSTLLNAPETDRRPLPHTVSFITAAAPPPAAVLAAMQEA
GFNVVHVYGLTEVYGPAVVNDWHGEWDALPPAERAAKKARQGVRYGALEALDVLDPLTMEPVPADGETIGEVMFRGNVVM
KGYVKNPAATDAAFAGGWFHSGDLGVKHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPAVSAAAVVARPDEKWGE
TPLAFVELKDGAAASAEDLIAHCRAHLAAYKCPRTILFEEIPKTSTGKIQKFKLRELAKGL

Sequences:

>Translated_541_residues
MTSIFDQHLDRNPANYQPLTPLGFLERAASVFPDQVAVIHGPLRRTYRELYARTRRLASALAQRGIGVGDTVAVMLSNTP
PMLEAHYGVPMCGAVLNTFNTRLDAAILAFTIDHGEAKVLITDREFSPVIKAALSLATRKPLVIDYDDPEYAGPGERLGA
IEYEDFIAAGDPDFVWAPPADEWDAISLNYTSGTTGDPKGVVYHHRGAHLLSVGNVVTCHMGPHPVYLWTLPMFHCNGWC
FPWSVSVVAGTHVCLRQVRAKAIYEAIEDHKVTHMCGAPIVMSTLLNAPETDRRPLPHTVSFITAAAPPPAAVLAAMQEA
GFNVVHVYGLTEVYGPAVVNDWHGEWDALPPAERAAKKARQGVRYGALEALDVLDPLTMEPVPADGETIGEVMFRGNVVM
KGYVKNPAATDAAFAGGWFHSGDLGVKHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPAVSAAAVVARPDEKWGE
TPLAFVELKDGAAASAEDLIAHCRAHLAAYKCPRTILFEEIPKTSTGKIQKFKLRELAKGL
>Mature_540_residues
TSIFDQHLDRNPANYQPLTPLGFLERAASVFPDQVAVIHGPLRRTYRELYARTRRLASALAQRGIGVGDTVAVMLSNTPP
MLEAHYGVPMCGAVLNTFNTRLDAAILAFTIDHGEAKVLITDREFSPVIKAALSLATRKPLVIDYDDPEYAGPGERLGAI
EYEDFIAAGDPDFVWAPPADEWDAISLNYTSGTTGDPKGVVYHHRGAHLLSVGNVVTCHMGPHPVYLWTLPMFHCNGWCF
PWSVSVVAGTHVCLRQVRAKAIYEAIEDHKVTHMCGAPIVMSTLLNAPETDRRPLPHTVSFITAAAPPPAAVLAAMQEAG
FNVVHVYGLTEVYGPAVVNDWHGEWDALPPAERAAKKARQGVRYGALEALDVLDPLTMEPVPADGETIGEVMFRGNVVMK
GYVKNPAATDAAFAGGWFHSGDLGVKHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPAVSAAAVVARPDEKWGET
PLAFVELKDGAAASAEDLIAHCRAHLAAYKCPRTILFEEIPKTSTGKIQKFKLRELAKGL

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=565, Percent_Identity=26.7256637168142, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI187761345, Length=559, Percent_Identity=27.5491949910555, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI187761343, Length=559, Percent_Identity=27.5491949910555, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI42544132, Length=556, Percent_Identity=25.8992805755396, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI58082049, Length=512, Percent_Identity=25.78125, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI157311624, Length=515, Percent_Identity=26.2135922330097, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI157311622, Length=515, Percent_Identity=26.2135922330097, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI122937307, Length=523, Percent_Identity=23.9005736137667, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI115511026, Length=526, Percent_Identity=25.4752851711027, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI38505220, Length=519, Percent_Identity=26.0115606936416, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI28416953, Length=547, Percent_Identity=24.8628884826325, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI221142682, Length=503, Percent_Identity=27.037773359841, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1788107, Length=568, Percent_Identity=26.7605633802817, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI145693145, Length=526, Percent_Identity=29.277566539924, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1786810, Length=537, Percent_Identity=29.608938547486, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790505, Length=542, Percent_Identity=22.6937269372694, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71994703, Length=556, Percent_Identity=28.2374100719424, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI71994690, Length=556, Percent_Identity=28.2374100719424, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI71994694, Length=556, Percent_Identity=28.2374100719424, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17560308, Length=539, Percent_Identity=27.4582560296846, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17558820, Length=511, Percent_Identity=28.1800391389432, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17559526, Length=513, Percent_Identity=25.7309941520468, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32563687, Length=518, Percent_Identity=27.027027027027, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=520, Percent_Identity=26.1538461538462, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=525, Percent_Identity=26.6666666666667, Blast_Score=128, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17531443, Length=527, Percent_Identity=22.3908918406072, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=533, Percent_Identity=23.0769230769231, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=520, Percent_Identity=24.0384615384615, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=360, Percent_Identity=26.1111111111111, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71982997, Length=548, Percent_Identity=24.8175182481752, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71983001, Length=548, Percent_Identity=24.8175182481752, Blast_Score=79, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=491, Percent_Identity=26.4765784114053, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6319264, Length=560, Percent_Identity=24.2857142857143, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323182, Length=138, Percent_Identity=34.0579710144928, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24581924, Length=579, Percent_Identity=26.7702936096718, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI18859661, Length=511, Percent_Identity=26.8101761252446, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI21355181, Length=502, Percent_Identity=26.4940239043825, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21356947, Length=549, Percent_Identity=22.5865209471767, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI45550496, Length=543, Percent_Identity=25.0460405156538, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45552801, Length=543, Percent_Identity=25.0460405156538, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24648253, Length=522, Percent_Identity=21.8390804597701, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648255, Length=522, Percent_Identity=22.6053639846743, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI20130357, Length=399, Percent_Identity=24.0601503759398, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21358303, Length=246, Percent_Identity=24.7967479674797, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 58746; Mature: 58614

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIFDQHLDRNPANYQPLTPLGFLERAASVFPDQVAVIHGPLRRTYRELYARTRRLASA
CCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
LAQRGIGVGDTVAVMLSNTPPMLEAHYGVPMCGAVLNTFNTRLDAAILAFTIDHGEAKVL
HHHCCCCCCCEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHCEEEEEEEEECCCEEEE
ITDREFSPVIKAALSLATRKPLVIDYDDPEYAGPGERLGAIEYEDFIAAGDPDFVWAPPA
EECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHCCCEEHHHHEECCCCCEEECCCC
DEWDAISLNYTSGTTGDPKGVVYHHRGAHLLSVGNVVTCHMGPHPVYLWTLPMFHCNGWC
CCCCEEEEEECCCCCCCCCCEEEECCCCEEEEECCEEEEECCCCEEEEEEEHHHHCCCCC
FPWSVSVVAGTHVCLRQVRAKAIYEAIEDHKVTHMCGAPIVMSTLLNAPETDRRPLPHTV
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCCCCCCCCCCCHH
SFITAAAPPPAAVLAAMQEAGFNVVHVYGLTEVYGPAVVNDWHGEWDALPPAERAAKKAR
HHEEECCCCHHHHHHHHHHCCCCEEEEEECHHHCCCCEEECCCCCCCCCCCHHHHHHHHH
QGVRYGALEALDVLDPLTMEPVPADGETIGEVMFRGNVVMKGYVKNPAATDAAFAGGWFH
CCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHCCCCEE
SGDLGVKHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPAVSAAAVVARPDEKWGE
CCCCCCCCCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHCCCCCCEEEEEECCCHHHCC
TPLAFVELKDGAAASAEDLIAHCRAHLAAYKCPRTILFEEIPKTSTGKIQKFKLRELAKG
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHC
L
C
>Mature Secondary Structure 
TSIFDQHLDRNPANYQPLTPLGFLERAASVFPDQVAVIHGPLRRTYRELYARTRRLASA
CCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
LAQRGIGVGDTVAVMLSNTPPMLEAHYGVPMCGAVLNTFNTRLDAAILAFTIDHGEAKVL
HHHCCCCCCCEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHCEEEEEEEEECCCEEEE
ITDREFSPVIKAALSLATRKPLVIDYDDPEYAGPGERLGAIEYEDFIAAGDPDFVWAPPA
EECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHCCCEEHHHHEECCCCCEEECCCC
DEWDAISLNYTSGTTGDPKGVVYHHRGAHLLSVGNVVTCHMGPHPVYLWTLPMFHCNGWC
CCCCEEEEEECCCCCCCCCCEEEECCCCEEEEECCEEEEECCCCEEEEEEEHHHHCCCCC
FPWSVSVVAGTHVCLRQVRAKAIYEAIEDHKVTHMCGAPIVMSTLLNAPETDRRPLPHTV
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCCCCCCCCCCCHH
SFITAAAPPPAAVLAAMQEAGFNVVHVYGLTEVYGPAVVNDWHGEWDALPPAERAAKKAR
HHEEECCCCHHHHHHHHHHCCCCEEEEEECHHHCCCCEEECCCCCCCCCCCHHHHHHHHH
QGVRYGALEALDVLDPLTMEPVPADGETIGEVMFRGNVVMKGYVKNPAATDAAFAGGWFH
CCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHCCCCEE
SGDLGVKHPDGYIQLKDRSKDIIISGGENISSIEVEDALYKHPAVSAAAVVARPDEKWGE
CCCCCCCCCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHCCCCCCEEEEEECCCHHHCC
TPLAFVELKDGAAASAEDLIAHCRAHLAAYKCPRTILFEEIPKTSTGKIQKFKLRELAKG
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]