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The map label for this gene is clcA [H]

Identifier: 158423098

GI number: 158423098

Start: 1673158

End: 1674492

Strand: Reverse

Name: clcA [H]

Synonym: AZC_1474

Alternate gene names: 158423098

Gene position: 1674492-1673158 (Counterclockwise)

Preceding gene: 158423099

Following gene: 158423095

Centisome position: 31.18

GC content: 69.81

Gene sequence:

>1335_bases
ATGTTCAGACGCGACCTGCGGCGGATTCGCTTTGCGCTCACCTCCCGGATGCTCTGGAAGCGCCGGGCGGTGTTCCTGCT
GGGCGCGGTGGCGGTCGGCGTCGCCTCCGTCGCCTTCGCCAAGATGGCGGACGGGGCGCAGGAGTTCTATGTCCACACCA
TGGAACGCATGCCCTATGCGTTCCTCATCCTGACGCCGCTGGGCTTCGGCCTGTTCGCCTGGGCCACCGCCCGCTTCTTC
CCCGGTGCGCAGGGCAGCGGCATTCCGCAGGCCATCGCCGCCCGAAGGTCCGGCAGTCCGGCCCACCGCGCCCGCCTGCT
CTCCGCGCGCGTCACCATCGGCAAGGTGCTCCTCACCTGCCTCGGCCTTGCGGTCGGCGGCTCCATCGGCCGCGAGGGGC
CGACCGTGCAGCTCGGCTGCGCCATCATGGTGGCGCTCGCCGCCTTCGCCGGCATCGGCCGCGCCAACGGCCTCATTCTG
GTGGGTGCGGCGGCGGGCATTGCGGGCGCCTTCAACACGCCGCTCGCCGGCGTCATGTTCGCCATCGAGGAGATGGCGAA
GTCCTATGACAAGCGCCTGTCCGGCCTCATCGCCGCCGCCGTGGTCATCTCCGGCATGGTGAGCCTGCTGCTGGTGGGCC
AGTATCTCTATTTCGGCTCCATCCCGACGGAAGCCTTCGCGACCATCGGCTGGAATGCCGTGGTGTTCTGCGCCGTGGTG
GGGGGCGTCGGCGGCGCCATCTTCTCGCGTGGCCTCGTGGCGCTTTCCAATGCGAAGGGCCGCATCCTCGGCCCGCTGCG
CAAGCGCCCCTTCGTCTATGGCGCGCTCTGCGGCCTCATCGTCGGCGCGCTCGCCATCCTCACCCACGGCTTTGCCGGCG
GCTCGGGCTACGGGCCGACGCGGCTGCTCCTGGAGGAAGGCGTCGCCTCGCCCTGGTGGTACGCGCCGATCAAGCTGTTC
ACGACGCTCCTGTCCTCCGCCAGCGGCATTCCCGGCGGCCTGTTCTCGCCGTCGCTGTCGGTCGGCGCCAACATCGGCGC
CGCGCTCGCGCCCTTCATGCCGGAGATGGACATCCGCGCCCTCGCCATGCTGGGCATGGTCGGCTATTTCGCCGGCGTGG
TGCAGGCGCCTTTGACCGCCTTCGTCATCGTGATGGAAATGACCAACGAGGTGCACCTCGTGGTGCCCATGCTGGCGACG
GCATTGCTCGGCGCCGGCATCTCCCGCGTGCTGGCGCCCGAGCCGCTCTATCACGCCCTCTCGTTCGCCTATGACCCGAG
GCCCGAGGAAATGCCGCAGGCAGGCGAAGCCAAGGCCGACACCAAGGCCCCCTGA

Upstream 100 bases:

>100_bases
GCTCGCCATCTTCGCCCGTAACGCCTGTTGAACCTCCGCGCTGCGGCGGGGGCCAGGGGCGGGGCGCAGGACGAGGCGGC
CGGCCGGAAGGATCAGGGGC

Downstream 100 bases:

>100_bases
AGTGCCCGCGGGCGGGGGCCGCGCGGAGACCTGAGACTCAGCGCAACTGCGCTCCGGTGGCGAGCAGGATCTGCGGCAGC
TGGGCGCCGGCGAGGACCTC

Product: chloride channel protein

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MFRRDLRRIRFALTSRMLWKRRAVFLLGAVAVGVASVAFAKMADGAQEFYVHTMERMPYAFLILTPLGFGLFAWATARFF
PGAQGSGIPQAIAARRSGSPAHRARLLSARVTIGKVLLTCLGLAVGGSIGREGPTVQLGCAIMVALAAFAGIGRANGLIL
VGAAAGIAGAFNTPLAGVMFAIEEMAKSYDKRLSGLIAAAVVISGMVSLLLVGQYLYFGSIPTEAFATIGWNAVVFCAVV
GGVGGAIFSRGLVALSNAKGRILGPLRKRPFVYGALCGLIVGALAILTHGFAGGSGYGPTRLLLEEGVASPWWYAPIKLF
TTLLSSASGIPGGLFSPSLSVGANIGAALAPFMPEMDIRALAMLGMVGYFAGVVQAPLTAFVIVMEMTNEVHLVVPMLAT
ALLGAGISRVLAPEPLYHALSFAYDPRPEEMPQAGEAKADTKAP

Sequences:

>Translated_444_residues
MFRRDLRRIRFALTSRMLWKRRAVFLLGAVAVGVASVAFAKMADGAQEFYVHTMERMPYAFLILTPLGFGLFAWATARFF
PGAQGSGIPQAIAARRSGSPAHRARLLSARVTIGKVLLTCLGLAVGGSIGREGPTVQLGCAIMVALAAFAGIGRANGLIL
VGAAAGIAGAFNTPLAGVMFAIEEMAKSYDKRLSGLIAAAVVISGMVSLLLVGQYLYFGSIPTEAFATIGWNAVVFCAVV
GGVGGAIFSRGLVALSNAKGRILGPLRKRPFVYGALCGLIVGALAILTHGFAGGSGYGPTRLLLEEGVASPWWYAPIKLF
TTLLSSASGIPGGLFSPSLSVGANIGAALAPFMPEMDIRALAMLGMVGYFAGVVQAPLTAFVIVMEMTNEVHLVVPMLAT
ALLGAGISRVLAPEPLYHALSFAYDPRPEEMPQAGEAKADTKAP
>Mature_444_residues
MFRRDLRRIRFALTSRMLWKRRAVFLLGAVAVGVASVAFAKMADGAQEFYVHTMERMPYAFLILTPLGFGLFAWATARFF
PGAQGSGIPQAIAARRSGSPAHRARLLSARVTIGKVLLTCLGLAVGGSIGREGPTVQLGCAIMVALAAFAGIGRANGLIL
VGAAAGIAGAFNTPLAGVMFAIEEMAKSYDKRLSGLIAAAVVISGMVSLLLVGQYLYFGSIPTEAFATIGWNAVVFCAVV
GGVGGAIFSRGLVALSNAKGRILGPLRKRPFVYGALCGLIVGALAILTHGFAGGSGYGPTRLLLEEGVASPWWYAPIKLF
TTLLSSASGIPGGLFSPSLSVGANIGAALAPFMPEMDIRALAMLGMVGYFAGVVQAPLTAFVIVMEMTNEVHLVVPMLAT
ALLGAGISRVLAPEPLYHALSFAYDPRPEEMPQAGEAKADTKAP

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786350, Length=452, Percent_Identity=30.0884955752212, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 46221; Mature: 46221

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS00659 GLYCOSYL_HYDROL_F5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRRDLRRIRFALTSRMLWKRRAVFLLGAVAVGVASVAFAKMADGAQEFYVHTMERMPYA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEE
FLILTPLGFGLFAWATARFFPGAQGSGIPQAIAARRSGSPAHRARLLSARVTIGKVLLTC
EEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LGLAVGGSIGREGPTVQLGCAIMVALAAFAGIGRANGLILVGAAAGIAGAFNTPLAGVMF
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHCCCCCCHHHHHHH
AIEEMAKSYDKRLSGLIAAAVVISGMVSLLLVGQYLYFGSIPTEAFATIGWNAVVFCAVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHH
GGVGGAIFSRGLVALSNAKGRILGPLRKRPFVYGALCGLIVGALAILTHGFAGGSGYGPT
HCCHHHHHHCCHHEECCCCCCEECHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
RLLLEEGVASPWWYAPIKLFTTLLSSASGIPGGLFSPSLSVGANIGAALAPFMPEMDIRA
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCHHHHHCCCCCHHHHH
LAMLGMVGYFAGVVQAPLTAFVIVMEMTNEVHLVVPMLATALLGAGISRVLAPEPLYHAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
SFAYDPRPEEMPQAGEAKADTKAP
HHCCCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MFRRDLRRIRFALTSRMLWKRRAVFLLGAVAVGVASVAFAKMADGAQEFYVHTMERMPYA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEE
FLILTPLGFGLFAWATARFFPGAQGSGIPQAIAARRSGSPAHRARLLSARVTIGKVLLTC
EEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LGLAVGGSIGREGPTVQLGCAIMVALAAFAGIGRANGLILVGAAAGIAGAFNTPLAGVMF
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHCCCCCCHHHHHHH
AIEEMAKSYDKRLSGLIAAAVVISGMVSLLLVGQYLYFGSIPTEAFATIGWNAVVFCAVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHH
GGVGGAIFSRGLVALSNAKGRILGPLRKRPFVYGALCGLIVGALAILTHGFAGGSGYGPT
HCCHHHHHHCCHHEECCCCCCEECHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
RLLLEEGVASPWWYAPIKLFTTLLSSASGIPGGLFSPSLSVGANIGAALAPFMPEMDIRA
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCHHHHHCCCCCHHHHH
LAMLGMVGYFAGVVQAPLTAFVIVMEMTNEVHLVVPMLATALLGAGISRVLAPEPLYHAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
SFAYDPRPEEMPQAGEAKADTKAP
HHCCCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA