Click here to switch to the map view.

The map label for this gene is speE [H]

Identifier: 157963294

GI number: 157963294

Start: 4252242

End: 4253969

Strand: Direct

Name: speE [H]

Synonym: Spea_3480

Alternate gene names: 157963294

Gene position: 4252242-4253969 (Clockwise)

Preceding gene: 157963293

Following gene: 157963295

Centisome position: 82.18

GC content: 49.02

Gene sequence:

>1728_bases
ATGCAAACCGACTCAAATAACTCCACCACCGAATCTAGCAACCCAACCATAGAATCAGGCAAATTAGGCTGGTTCGATGA
TGTGCTTCTGCTTGGCATCATGGCCGTGCTCGCTGGATGCGGCCTTATCTATGAGTATCTACTCTCTCATTATGCCGGAC
GCATTCTAGGCGCCCTCGAAGCTGCTATTTACACCATGATAGGTCTGATGATCGTATCCATGGGGCTCGGCGCCTTTGCC
GCCCGCAAAATTCGCTGCGCCTTTACTGGCTTTGCCGTGTTGGAACTTTGCGTCGCGCTTTGTGGATCGCTAGCAATCTT
AATTACTGCAGCCGTTATCGGCTTTGGCCAGCAGCTTCCCCTTGTTATCGCCAATACTCTTGGCCTACCTGCGGATCAAC
TGCCTGATGGTGGTTTTATTGGCACCTTGCAAAAGCTCAGTGAATATTTGCCTTATGTGTGGGGCGTGCTACTAGGCCTG
ATGATAGGCATGGAGATCCCACTGATCGCACGCGTGCGCCAAGCACTATCAGAGGCGCACTTAATGCACAACGCTGGCAC
CATCTATGGCGCTGATTATGTCGGTGCGGGTATCGGCGCTGCCATTTGGGTTGGTTTTATGTTGGCAATTGATATTCAGC
TAGCGGCGGCGCTGACTGCCAGTTTTAATTTGTTAGCCGGCTTTATCTTTATTTGGCGCTTCTGGCATCAAATTCGCCAT
GTAAAACTGCTCCTCGTTGGCCACTTTATCGCAACGGGGATCTTAGTGTTATTGGCGCTACACGGGCCATCATGGGAACA
GAGCTTTAATAACCTGCTCTATAAAGACAAGGTGGTTTATGCCAAGGCGACTCGCTTTCAACAGCTGACCTTTACTGAGC
GTCTTAGAGGCAACGGTCTTGGCCCTGTGCATTCGCTGTATATCAACGGACGATTGCAGTTCTCCAGTAGCGATGAGCAT
ATCTATCACTCTTTCTTGGTACACCCGACCATGGCGGCTAGCGCACGTCACGACAAGGTGTTAATCATTGGCGGCGGCGA
TGGCTTAGGACTTACCCAAGTGTTGCAATGGCAACCAGAGCAGGTGACGCTAATGGATCTTGATAAGGAACTGGTTGAGC
TGTTTAAGCAACCCGCTAGCGATATGCCCGATCATTTAAGCCGAGCACTACTCGCACTCAACCAAGATGCGCTAAACGAC
CCTCGAGTAAGAATTATGTATGAGGATGCCTTTAACGGAGTTGATAAGTTATTAGCTAAGGAAGAGAAGTTCGATGTCAT
TATTGTCGATCTGCCCGACCCGAGTCATCCAGATCTTAACAAGCTGTATTCCGATGCCTTCTATTTAAAGCTCAAGGAAC
TAATGAGTAATGACGCTGCCTTAACCATACAGTCGACGTCACCTTATCACGCCCCTAACGCCTTTATTTCGGTAGGTAAG
ACCTTGGAGCTTGCTGGCTTTAGAGTCGATCAGTACCACCATAACGTACCCAGTTTTGGTGAATGGGGCTGGAGCATTGC
TACCTTAGGTGGAAAAAGTGCCCGCACTAGGCTACAAAGTCTGCAGCAGCTGCCTGTCGATGACCCTTGGTTAACTCCAG
GTCTAATCCATGCAGCCTTTGAGTTTCCGGGGAATTTTTACCAGAACAAAGCAGAAATTAAGCCCAATGTTTTAGGTTCA
AGCCAGCTGTATCAATATCATCAGCAGGCTTGGTTAGAAAATCAGTAG

Upstream 100 bases:

>100_bases
GAGCGCTTAATACGCTCCCTTTTCTATTACTTATTTCGTCATCATCCATTAAAACGATCTCTCACAAAACCATCTCTAAT
AAATAAGCAACTGACACCCA

Downstream 100 bases:

>100_bases
AGTTTATTTTTGAATCGTAAAGATAGCCCTTGACATATTATGTCGTAGTGTTATCTTTAATCACAGACATAATATGTCAA
TAAGGACAAATATGAATATC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MQTDSNNSTTESSNPTIESGKLGWFDDVLLLGIMAVLAGCGLIYEYLLSHYAGRILGALEAAIYTMIGLMIVSMGLGAFA
ARKIRCAFTGFAVLELCVALCGSLAILITAAVIGFGQQLPLVIANTLGLPADQLPDGGFIGTLQKLSEYLPYVWGVLLGL
MIGMEIPLIARVRQALSEAHLMHNAGTIYGADYVGAGIGAAIWVGFMLAIDIQLAAALTASFNLLAGFIFIWRFWHQIRH
VKLLLVGHFIATGILVLLALHGPSWEQSFNNLLYKDKVVYAKATRFQQLTFTERLRGNGLGPVHSLYINGRLQFSSSDEH
IYHSFLVHPTMAASARHDKVLIIGGGDGLGLTQVLQWQPEQVTLMDLDKELVELFKQPASDMPDHLSRALLALNQDALND
PRVRIMYEDAFNGVDKLLAKEEKFDVIIVDLPDPSHPDLNKLYSDAFYLKLKELMSNDAALTIQSTSPYHAPNAFISVGK
TLELAGFRVDQYHHNVPSFGEWGWSIATLGGKSARTRLQSLQQLPVDDPWLTPGLIHAAFEFPGNFYQNKAEIKPNVLGS
SQLYQYHQQAWLENQ

Sequences:

>Translated_575_residues
MQTDSNNSTTESSNPTIESGKLGWFDDVLLLGIMAVLAGCGLIYEYLLSHYAGRILGALEAAIYTMIGLMIVSMGLGAFA
ARKIRCAFTGFAVLELCVALCGSLAILITAAVIGFGQQLPLVIANTLGLPADQLPDGGFIGTLQKLSEYLPYVWGVLLGL
MIGMEIPLIARVRQALSEAHLMHNAGTIYGADYVGAGIGAAIWVGFMLAIDIQLAAALTASFNLLAGFIFIWRFWHQIRH
VKLLLVGHFIATGILVLLALHGPSWEQSFNNLLYKDKVVYAKATRFQQLTFTERLRGNGLGPVHSLYINGRLQFSSSDEH
IYHSFLVHPTMAASARHDKVLIIGGGDGLGLTQVLQWQPEQVTLMDLDKELVELFKQPASDMPDHLSRALLALNQDALND
PRVRIMYEDAFNGVDKLLAKEEKFDVIIVDLPDPSHPDLNKLYSDAFYLKLKELMSNDAALTIQSTSPYHAPNAFISVGK
TLELAGFRVDQYHHNVPSFGEWGWSIATLGGKSARTRLQSLQQLPVDDPWLTPGLIHAAFEFPGNFYQNKAEIKPNVLGS
SQLYQYHQQAWLENQ
>Mature_575_residues
MQTDSNNSTTESSNPTIESGKLGWFDDVLLLGIMAVLAGCGLIYEYLLSHYAGRILGALEAAIYTMIGLMIVSMGLGAFA
ARKIRCAFTGFAVLELCVALCGSLAILITAAVIGFGQQLPLVIANTLGLPADQLPDGGFIGTLQKLSEYLPYVWGVLLGL
MIGMEIPLIARVRQALSEAHLMHNAGTIYGADYVGAGIGAAIWVGFMLAIDIQLAAALTASFNLLAGFIFIWRFWHQIRH
VKLLLVGHFIATGILVLLALHGPSWEQSFNNLLYKDKVVYAKATRFQQLTFTERLRGNGLGPVHSLYINGRLQFSSSDEH
IYHSFLVHPTMAASARHDKVLIIGGGDGLGLTQVLQWQPEQVTLMDLDKELVELFKQPASDMPDHLSRALLALNQDALND
PRVRIMYEDAFNGVDKLLAKEEKFDVIIVDLPDPSHPDLNKLYSDAFYLKLKELMSNDAALTIQSTSPYHAPNAFISVGK
TLELAGFRVDQYHHNVPSFGEWGWSIATLGGKSARTRLQSLQQLPVDDPWLTPGLIHAAFEFPGNFYQNKAEIKPNVLGS
SQLYQYHQQAWLENQ

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786312, Length=189, Percent_Identity=31.2169312169312, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323175, Length=198, Percent_Identity=28.2828282828283, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 63280; Mature: 63280

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTDSNNSTTESSNPTIESGKLGWFDDVLLLGIMAVLAGCGLIYEYLLSHYAGRILGALE
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAIYTMIGLMIVSMGLGAFAARKIRCAFTGFAVLELCVALCGSLAILITAAVIGFGQQLP
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
LVIANTLGLPADQLPDGGFIGTLQKLSEYLPYVWGVLLGLMIGMEIPLIARVRQALSEAH
HHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LMHNAGTIYGADYVGAGIGAAIWVGFMLAIDIQLAAALTASFNLLAGFIFIWRFWHQIRH
HHHCCCCEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKLLLVGHFIATGILVLLALHGPSWEQSFNNLLYKDKVVYAKATRFQQLTFTERLRGNGL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCC
GPVHSLYINGRLQFSSSDEHIYHSFLVHPTMAASARHDKVLIIGGGDGLGLTQVLQWQPE
CCCCEEEEEEEEEECCCCCHHHHHHHHCCHHHCCCCCCEEEEEECCCCCCHHHHHHCCCC
QVTLMDLDKELVELFKQPASDMPDHLSRALLALNQDALNDPRVRIMYEDAFNGVDKLLAK
EEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHC
EEKFDVIIVDLPDPSHPDLNKLYSDAFYLKLKELMSNDAALTIQSTSPYHAPNAFISVGK
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCC
TLELAGFRVDQYHHNVPSFGEWGWSIATLGGKSARTRLQSLQQLPVDDPWLTPGLIHAAF
EEEECCEEEHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
EFPGNFYQNKAEIKPNVLGSSQLYQYHQQAWLENQ
HCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQTDSNNSTTESSNPTIESGKLGWFDDVLLLGIMAVLAGCGLIYEYLLSHYAGRILGALE
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAIYTMIGLMIVSMGLGAFAARKIRCAFTGFAVLELCVALCGSLAILITAAVIGFGQQLP
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
LVIANTLGLPADQLPDGGFIGTLQKLSEYLPYVWGVLLGLMIGMEIPLIARVRQALSEAH
HHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LMHNAGTIYGADYVGAGIGAAIWVGFMLAIDIQLAAALTASFNLLAGFIFIWRFWHQIRH
HHHCCCCEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKLLLVGHFIATGILVLLALHGPSWEQSFNNLLYKDKVVYAKATRFQQLTFTERLRGNGL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCC
GPVHSLYINGRLQFSSSDEHIYHSFLVHPTMAASARHDKVLIIGGGDGLGLTQVLQWQPE
CCCCEEEEEEEEEECCCCCHHHHHHHHCCHHHCCCCCCEEEEEECCCCCCHHHHHHCCCC
QVTLMDLDKELVELFKQPASDMPDHLSRALLALNQDALNDPRVRIMYEDAFNGVDKLLAK
EEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHC
EEKFDVIIVDLPDPSHPDLNKLYSDAFYLKLKELMSNDAALTIQSTSPYHAPNAFISVGK
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCC
TLELAGFRVDQYHHNVPSFGEWGWSIATLGGKSARTRLQSLQQLPVDDPWLTPGLIHAAF
EEEECCEEEHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
EFPGNFYQNKAEIKPNVLGSSQLYQYHQQAWLENQ
HCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]