Click here to switch to the map view.

The map label for this gene is phaAB [H]

Identifier: 157963098

GI number: 157963098

Start: 4023415

End: 4024452

Strand: Direct

Name: phaAB [H]

Synonym: Spea_3282

Alternate gene names: 157963098

Gene position: 4023415-4024452 (Clockwise)

Preceding gene: 157963097

Following gene: 157963099

Centisome position: 77.75

GC content: 47.59

Gene sequence:

>1038_bases
ATGTTGACGGGGATCTATGGATTACTCTCTGCAAGCTTTTTTGTTGTATTAGATGCTGTGGATGTGGCCTTTACCGAAGC
CTCTGTAGGCGCAGGTATATCTGGTTTATTGATGCTGGCAGCCATCAGCATGACAGGCCGCACCGAGTCGCCTAAGCGTC
ACAAGCCTTTACTGGCTTTATTTGTGGTACTTATCACAGGTGGCATGCTGATCTACGGCACCATGGATATGCCTTACTTT
GGCAGTTTCGATGCACCTATTCATCAACATGTCGCACCTCGTTACATTAATGACTCGATGCAGGAGGTCGGTGTACCCAA
TATTGTCACCTCGGTACTCGCGAGCTACCGAGCATTTGATACCTTAGGTGAGGTAGCCGTTATCTTTACTGCCGGCATTG
GGGTGTTATCACTCTTGGCATTGCCGCAACGACGTAAGTCATATCGCTTAGATGAGCCCAAAAAAGAGCAGATGTATCAG
CAGCATATCGTACTGCGAGTCATCAGCAAAGCCTTGATCCCTTTCATCCTGCTGTTTGCACTCTATGTGCAGTTCCACGG
CGATTTTGGTCCCGGAGGGGGCTTTCAAGCTGGGGTGATCTTTGCCGCAGCAATCATTCTCTATGCCATGTTATTTGGCT
TAGACAATGCCAAGCGTGTCTTTAGTCTATCGCTGTTCCAACTGCTCGCAGCTATCGGACTCTTGCTATACGCCAGTGTT
GGTGTGGTCTCGCTATTTAACGGAGGTAACTTCTTAGATTACAACGTATTGGCCGACGACCCTATTGCTGGCCAGCACTT
AGGTATTTTGCTGATCGAGCTTGGTGTGGGCTGCACCGTCGCCTCAGTGATGATCATTATCTTTTTCAACTTTGCCGGCC
GCAGAGAAGCACAACAGCACTCTACTAAACAGAACTGTCATGAACTAATCAGTCCAGAACTAAACCGCAATGTTCTAAAT
AGTACTGAGCTAAACAGTACTGAACTAAACAGTGCTGAGCTAAGCATTACTGTACTAAACAACGGCGAGGTGAAGTAA

Upstream 100 bases:

>100_bases
GGAACTAGCCATCGAAACCTTAGTTGATGTCGTTCTATTAAGCTTTCTCGTCGTGATTGCTATCGCCATTGTTCGGACAA
AAGATCTATTGGCCGTAGTG

Downstream 100 bases:

>100_bases
TGATACTGGGACTCTATAACTACTGGATCGTGGTATTACTGATGATGATAGGTTTTTATATCGTCATCGCCCATGGCAAC
CTAATCAAAAAATTAGTTGG

Product: putative monovalent cation/H+ antiporter subunit B

Products: NA

Alternate protein names: pH adaptation potassium efflux system protein A/B; Pha system subunit A/B [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MLTGIYGLLSASFFVVLDAVDVAFTEASVGAGISGLLMLAAISMTGRTESPKRHKPLLALFVVLITGGMLIYGTMDMPYF
GSFDAPIHQHVAPRYINDSMQEVGVPNIVTSVLASYRAFDTLGEVAVIFTAGIGVLSLLALPQRRKSYRLDEPKKEQMYQ
QHIVLRVISKALIPFILLFALYVQFHGDFGPGGGFQAGVIFAAAIILYAMLFGLDNAKRVFSLSLFQLLAAIGLLLYASV
GVVSLFNGGNFLDYNVLADDPIAGQHLGILLIELGVGCTVASVMIIIFFNFAGRREAQQHSTKQNCHELISPELNRNVLN
STELNSTELNSAELSITVLNNGEVK

Sequences:

>Translated_345_residues
MLTGIYGLLSASFFVVLDAVDVAFTEASVGAGISGLLMLAAISMTGRTESPKRHKPLLALFVVLITGGMLIYGTMDMPYF
GSFDAPIHQHVAPRYINDSMQEVGVPNIVTSVLASYRAFDTLGEVAVIFTAGIGVLSLLALPQRRKSYRLDEPKKEQMYQ
QHIVLRVISKALIPFILLFALYVQFHGDFGPGGGFQAGVIFAAAIILYAMLFGLDNAKRVFSLSLFQLLAAIGLLLYASV
GVVSLFNGGNFLDYNVLADDPIAGQHLGILLIELGVGCTVASVMIIIFFNFAGRREAQQHSTKQNCHELISPELNRNVLN
STELNSTELNSAELSITVLNNGEVK
>Mature_345_residues
MLTGIYGLLSASFFVVLDAVDVAFTEASVGAGISGLLMLAAISMTGRTESPKRHKPLLALFVVLITGGMLIYGTMDMPYF
GSFDAPIHQHVAPRYINDSMQEVGVPNIVTSVLASYRAFDTLGEVAVIFTAGIGVLSLLALPQRRKSYRLDEPKKEQMYQ
QHIVLRVISKALIPFILLFALYVQFHGDFGPGGGFQAGVIFAAAIILYAMLFGLDNAKRVFSLSLFQLLAAIGLLLYASV
GVVSLFNGGNFLDYNVLADDPIAGQHLGILLIELGVGCTVASVMIIIFFNFAGRREAQQHSTKQNCHELISPELNRNVLN
STELNSTELNSAELSITVLNNGEVK

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG2111

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhB subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the CPA3 antiporters (TC 2.A.63) subunit B family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR005281
- InterPro:   IPR007182
- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF04039 MnhB; PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: NA

Molecular weight: Translated: 37322; Mature: 37322

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTGIYGLLSASFFVVLDAVDVAFTEASVGAGISGLLMLAAISMTGRTESPKRHKPLLAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHCHHHHH
FVVLITGGMLIYGTMDMPYFGSFDAPIHQHVAPRYINDSMQEVGVPNIVTSVLASYRAFD
HHHHHHCCEEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
TLGEVAVIFTAGIGVLSLLALPQRRKSYRLDEPKKEQMYQQHIVLRVISKALIPFILLFA
HHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYVQFHGDFGPGGGFQAGVIFAAAIILYAMLFGLDNAKRVFSLSLFQLLAAIGLLLYASV
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GVVSLFNGGNFLDYNVLADDPIAGQHLGILLIELGVGCTVASVMIIIFFNFAGRREAQQH
HHHEEECCCCEEEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHH
STKQNCHELISPELNRNVLNSTELNSTELNSAELSITVLNNGEVK
HHHHHHHHHHCCHHCCHHCCCCCCCCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MLTGIYGLLSASFFVVLDAVDVAFTEASVGAGISGLLMLAAISMTGRTESPKRHKPLLAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHCHHHHH
FVVLITGGMLIYGTMDMPYFGSFDAPIHQHVAPRYINDSMQEVGVPNIVTSVLASYRAFD
HHHHHHCCEEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
TLGEVAVIFTAGIGVLSLLALPQRRKSYRLDEPKKEQMYQQHIVLRVISKALIPFILLFA
HHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYVQFHGDFGPGGGFQAGVIFAAAIILYAMLFGLDNAKRVFSLSLFQLLAAIGLLLYASV
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GVVSLFNGGNFLDYNVLADDPIAGQHLGILLIELGVGCTVASVMIIIFFNFAGRREAQQH
HHHEEECCCCEEEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHH
STKQNCHELISPELNRNVLNSTELNSTELNSAELSITVLNNGEVK
HHHHHHHHHHCCHHCCHHCCCCCCCCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]