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The map label for this gene is gltB [C]

Identifier: 157963044

GI number: 157963044

Start: 3955205

End: 3956749

Strand: Direct

Name: gltB [C]

Synonym: Spea_3227

Alternate gene names: 157963044

Gene position: 3955205-3956749 (Clockwise)

Preceding gene: 157963039

Following gene: 157963047

Centisome position: 76.44

GC content: 49.26

Gene sequence:

>1545_bases
ATGAAGCCAATAGTCGCAGATAATAAGCCCATAAAAGTCAGCCTCACTCAAGGAAAGGAGTACTACTTCTGCAGTTGTGG
GCGATCTAAGAATCAACCCTTCTGCGATGGCTCCCACGCAGGAACCCCATTCAAACCTCAGCAATTTATTGCAGACAAAA
CTGAAGATTCTTATCTGTGCCAGTGCAAACACACAGCAAACTCGCCCTTCTGTGATGGATCTCATAAGCAATTCACTCAG
AGCGACATTGGAAAACAGGGAAAAGAAAAAGAGACAACGACAACAAGCCAATCGGTTCCCATTGCTCACAATACGGCTGA
AGAGCCAACGCTTGAATATATTCACATGCTAGCCCGAGAAGGCTTATCGAAAGTGGGCCATCACGGCCCCATGACATCCA
TGGGCGTGCCACGCAATCAGTTGCCGCATTGGGACGACTTGCAGCTCATGGTGGCGCAGATGGCCACTAAACCCTTAATG
GAAGATGTTGAGGTGACAACCGAGCTCATCATAGGGCCGCAGGCAAGAAAGCCTCTCAAGCTCGATATCCCATTATTCGT
CTCCGATATGAGCTTTGGTTCATTATCTGAGGAGGCAAAGATTGCCCTCGCTCGCGGCGCCGAATTAGCGGGAACAGGGA
TCTGCTCTGGTGAGGGCGGCATATTGAGCGATGAACAAGCCGAGAATTCACGTTATTTTTATGAGTTAGCCAGCGCTAAG
TTTGGCTATAAAGAAGCCTTGCTCTGCAAAGTACAAAGCTTTCACTTTAAGGGCGGGCAAGGGGCAAAAACAGGCACAGG
TGGTCATCTGCCAGCTAGCAAAAATGTCGGCAAAATATCTGAAGTTCGCGGTTTGCCTGAGGGCGTCGACGCCATATCTC
CACCAACCTTTACCGAGTTAAAAAGCAGTGCTGACTTTAAACGTTTTGCCGACAGGGTTAGAGAGGTTTCAGGCGGGATC
CCAATCGGCTTTAAGCTCAGTGCCAATCATATCGAGCGCGATATCCAATTCGCCCTAGACGCCAGTGCCGACTACATCAT
ACTCGATGGTAGAGGTGGCGGAACGGGAGCCGCACCGGAAATTTTTCGCGATCATATCAGCGTACCAACGATTCCAGCCC
TTGCTAGAGCCAGAAGGTACCTTGACCAAAAAGGTGAGAGTGGTCGAGTGACCTTAATCGCCACTGGTGGTATACGCACT
CCCATCGACTTTGTAAAAGCCATGGCCTTAGGCGCCGATGGCGTCGCGGTATCTAATAGCGCCATGCAGGCTATTGGCTG
CGTGGCGGCAAGAATGTGCAATACCAACAACTGCCCAGCAGGGATTGCCACTCAAAAGTCAGATCTTCGTCAAAGGCTCA
ATATTGATAAATCAGCCAAACAGTTACACAACTTTTTTGAAGCCTCTGTCGAGCTTATGCAGGTAATGGCCCGTGCCTGT
GGTCATGACTCTCTCAGCCAGTTTAATAAAGATGACTTAGCCACCTGGCATATTGAGATGGCTCGCTTAACTGGGGTTAA
ATATTCCGGATTTTGTGAACCTTAA

Upstream 100 bases:

>100_bases
TAAGAGCAATCACACATAAGTTCAGGGCTAAAACCATCAAGTTCACCGATTAATGACTATGCTTTATGCTGTTCACTTAA
AACTAAATCTGGGGTAAAGC

Downstream 100 bases:

>100_bases
TAACTGTCATTGGCCCATATTCCCTTGTAGGCTTCTCAATGAAAACCTGACTCTGCTGGGTTTTTTCATTACGGCTACCC
ATGGCAAAGATGCTTGAAAT

Product: ferredoxin-dependent glutamate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKPIVADNKPIKVSLTQGKEYYFCSCGRSKNQPFCDGSHAGTPFKPQQFIADKTEDSYLCQCKHTANSPFCDGSHKQFTQ
SDIGKQGKEKETTTTSQSVPIAHNTAEEPTLEYIHMLAREGLSKVGHHGPMTSMGVPRNQLPHWDDLQLMVAQMATKPLM
EDVEVTTELIIGPQARKPLKLDIPLFVSDMSFGSLSEEAKIALARGAELAGTGICSGEGGILSDEQAENSRYFYELASAK
FGYKEALLCKVQSFHFKGGQGAKTGTGGHLPASKNVGKISEVRGLPEGVDAISPPTFTELKSSADFKRFADRVREVSGGI
PIGFKLSANHIERDIQFALDASADYIILDGRGGGTGAAPEIFRDHISVPTIPALARARRYLDQKGESGRVTLIATGGIRT
PIDFVKAMALGADGVAVSNSAMQAIGCVAARMCNTNNCPAGIATQKSDLRQRLNIDKSAKQLHNFFEASVELMQVMARAC
GHDSLSQFNKDDLATWHIEMARLTGVKYSGFCEP

Sequences:

>Translated_514_residues
MKPIVADNKPIKVSLTQGKEYYFCSCGRSKNQPFCDGSHAGTPFKPQQFIADKTEDSYLCQCKHTANSPFCDGSHKQFTQ
SDIGKQGKEKETTTTSQSVPIAHNTAEEPTLEYIHMLAREGLSKVGHHGPMTSMGVPRNQLPHWDDLQLMVAQMATKPLM
EDVEVTTELIIGPQARKPLKLDIPLFVSDMSFGSLSEEAKIALARGAELAGTGICSGEGGILSDEQAENSRYFYELASAK
FGYKEALLCKVQSFHFKGGQGAKTGTGGHLPASKNVGKISEVRGLPEGVDAISPPTFTELKSSADFKRFADRVREVSGGI
PIGFKLSANHIERDIQFALDASADYIILDGRGGGTGAAPEIFRDHISVPTIPALARARRYLDQKGESGRVTLIATGGIRT
PIDFVKAMALGADGVAVSNSAMQAIGCVAARMCNTNNCPAGIATQKSDLRQRLNIDKSAKQLHNFFEASVELMQVMARAC
GHDSLSQFNKDDLATWHIEMARLTGVKYSGFCEP
>Mature_514_residues
MKPIVADNKPIKVSLTQGKEYYFCSCGRSKNQPFCDGSHAGTPFKPQQFIADKTEDSYLCQCKHTANSPFCDGSHKQFTQ
SDIGKQGKEKETTTTSQSVPIAHNTAEEPTLEYIHMLAREGLSKVGHHGPMTSMGVPRNQLPHWDDLQLMVAQMATKPLM
EDVEVTTELIIGPQARKPLKLDIPLFVSDMSFGSLSEEAKIALARGAELAGTGICSGEGGILSDEQAENSRYFYELASAK
FGYKEALLCKVQSFHFKGGQGAKTGTGGHLPASKNVGKISEVRGLPEGVDAISPPTFTELKSSADFKRFADRVREVSGGI
PIGFKLSANHIERDIQFALDASADYIILDGRGGGTGAAPEIFRDHISVPTIPALARARRYLDQKGESGRVTLIATGGIRT
PIDFVKAMALGADGVAVSNSAMQAIGCVAARMCNTNNCPAGIATQKSDLRQRLNIDKSAKQLHNFFEASVELMQVMARAC
GHDSLSQFNKDDLATWHIEMARLTGVKYSGFCEP

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI211057411, Length=85, Percent_Identity=47.0588235294118, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI308199519, Length=293, Percent_Identity=33.1058020477816, Blast_Score=112, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17570289, Length=277, Percent_Identity=31.7689530685921, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI115534146, Length=78, Percent_Identity=50, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556380, Length=79, Percent_Identity=41.7721518987342, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=306, Percent_Identity=31.3725490196078, Blast_Score=116, Evalue=8e-27,
Organism=Drosophila melanogaster, GI28574881, Length=295, Percent_Identity=31.5254237288136, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24665539, Length=295, Percent_Identity=31.5254237288136, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI28573970, Length=77, Percent_Identity=45.4545454545455, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 55582; Mature: 55582

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIVADNKPIKVSLTQGKEYYFCSCGRSKNQPFCDGSHAGTPFKPQQFIADKTEDSYLC
CCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEE
QCKHTANSPFCDGSHKQFTQSDIGKQGKEKETTTTSQSVPIAHNTAEEPTLEYIHMLARE
EEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
GLSKVGHHGPMTSMGVPRNQLPHWDDLQLMVAQMATKPLMEDVEVTTELIIGPQARKPLK
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEE
LDIPLFVSDMSFGSLSEEAKIALARGAELAGTGICSGEGGILSDEQAENSRYFYELASAK
ECCCEEEECCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCEEEEHHHCC
FGYKEALLCKVQSFHFKGGQGAKTGTGGHLPASKNVGKISEVRGLPEGVDAISPPTFTEL
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHH
KSSADFKRFADRVREVSGGIPIGFKLSANHIERDIQFALDASADYIILDGRGGGTGAAPE
HCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHEEECCCCEEEEECCCCCCCCCHH
IFRDHISVPTIPALARARRYLDQKGESGRVTLIATGGIRTPIDFVKAMALGADGVAVSNS
HHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEECHH
AMQAIGCVAARMCNTNNCPAGIATQKSDLRQRLNIDKSAKQLHNFFEASVELMQVMARAC
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
GHDSLSQFNKDDLATWHIEMARLTGVKYSGFCEP
CCHHHHHCCCCCCEEEEEEEHHHCCCCCCCCCCC
>Mature Secondary Structure
MKPIVADNKPIKVSLTQGKEYYFCSCGRSKNQPFCDGSHAGTPFKPQQFIADKTEDSYLC
CCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEE
QCKHTANSPFCDGSHKQFTQSDIGKQGKEKETTTTSQSVPIAHNTAEEPTLEYIHMLARE
EEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
GLSKVGHHGPMTSMGVPRNQLPHWDDLQLMVAQMATKPLMEDVEVTTELIIGPQARKPLK
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEE
LDIPLFVSDMSFGSLSEEAKIALARGAELAGTGICSGEGGILSDEQAENSRYFYELASAK
ECCCEEEECCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCEEEEHHHCC
FGYKEALLCKVQSFHFKGGQGAKTGTGGHLPASKNVGKISEVRGLPEGVDAISPPTFTEL
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHH
KSSADFKRFADRVREVSGGIPIGFKLSANHIERDIQFALDASADYIILDGRGGGTGAAPE
HCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHEEECCCCEEEEECCCCCCCCCHH
IFRDHISVPTIPALARARRYLDQKGESGRVTLIATGGIRTPIDFVKAMALGADGVAVSNS
HHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEECHH
AMQAIGCVAARMCNTNNCPAGIATQKSDLRQRLNIDKSAKQLHNFFEASVELMQVMARAC
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
GHDSLSQFNKDDLATWHIEMARLTGVKYSGFCEP
CCHHHHHCCCCCCEEEEEEEHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]