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The map label for this gene is mltB [H]

Identifier: 157962968

GI number: 157962968

Start: 3856668

End: 3857666

Strand: Direct

Name: mltB [H]

Synonym: Spea_3151

Alternate gene names: 157962968

Gene position: 3856668-3857666 (Clockwise)

Preceding gene: 157962967

Following gene: 157962969

Centisome position: 74.53

GC content: 44.34

Gene sequence:

>999_bases
ATGACACTATTAAAACGCATGCTTGCCCCTTTGGCATTAACCTTGTTTTCATGCTCAGCTGCAGCAGCACCTTCAGACTC
TGAAGTCGAAGCACTAAAAGCCGAATTTATCAAAACCCAGAGTGGCAAGGGGTTTGATGCCGAAGACACCACTATTTTCA
TCAAAAATGCAAAGTATAACCAAGCCGTTATCGATGCCATGACCAAGCCTTGGGAAGCCAAACCTTGGCATCAGTATTAT
CCAATATTCTTAACTGAAAAGCGTCTAGATGCGGGTCTTGCCTTCTGGAAAGAGCACGCAAAAACTATCGAACGAGCATC
AAAAGAGTTTAATGTCGATCCACAGATTATTGTGGCCATTATCGGCATCGAAACTTTTTATGGTGGTTACATGGGTAATT
ACCCTGTTAAAGACGCACTCTATACCTTAGGTTTCCATTATCCACCACGCGCCACCTTTTTTAGAAGTGAATTCGCAAAT
CTGCAGTCGCTAGTAAAAGAAGAGCGCTTAGATATCAATAGCCTCAAAGGCTCCTACGCAGGCGCAATGGGCTTTGGTCA
GTTTATACCGTCAAGCTACCGCCATTACGCGGTCGATTTTGATAATGACGGCAGCCGCGATCTACTTATCAGCACCACAG
ATGCCATTGGTAGTGTGGCTAACTATTTTCATCAGCATGGTTGGCAAAAAAACCAGCCAGTAACTCTTCAATTAACCTTG
AATAAAGACTTACCTGATACAGTCAAAACCTGGTCTGGGGAGAAACTGCATTATCAGGTTGCCGATATCCTATCGCCAAA
TGTCGCACTTGCTGAATCAATAGATCTCGACGTATCGCAACCGGCTCTAGTGGTTAAACTCGAGCAAGCCGAACATGATG
AGTATTGGCTAGGCCTTAAAAACTTTTATGTTATCACCCGTTACAACCGCAGCCCTCTGTATGCCATGGCCGTGTACCAG
TTTAGCCAGGAATTAAAGAAAGCCTATGCCACGCAATAA

Upstream 100 bases:

>100_bases
GACTAGTTCAAGCCATTTGATAATTAATCCTCGGAAGCATTATGGTGCATTCAAATCGCCTAACCATGATGCCTATAGAC
CACTAAGGACTGCACAATAG

Downstream 100 bases:

>100_bases
TACTCTCGCCATCTTGCTTTTAACTGCCACCCTAGCCGCATGTTCTAGTGGTGGTCGCTACGAACTTGATGACGATAAAG
CGCCGACTTCAGCCCCTGAT

Product: lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTLLKRMLAPLALTLFSCSAAAAPSDSEVEALKAEFIKTQSGKGFDAEDTTIFIKNAKYNQAVIDAMTKPWEAKPWHQYY
PIFLTEKRLDAGLAFWKEHAKTIERASKEFNVDPQIIVAIIGIETFYGGYMGNYPVKDALYTLGFHYPPRATFFRSEFAN
LQSLVKEERLDINSLKGSYAGAMGFGQFIPSSYRHYAVDFDNDGSRDLLISTTDAIGSVANYFHQHGWQKNQPVTLQLTL
NKDLPDTVKTWSGEKLHYQVADILSPNVALAESIDLDVSQPALVVKLEQAEHDEYWLGLKNFYVITRYNRSPLYAMAVYQ
FSQELKKAYATQ

Sequences:

>Translated_332_residues
MTLLKRMLAPLALTLFSCSAAAAPSDSEVEALKAEFIKTQSGKGFDAEDTTIFIKNAKYNQAVIDAMTKPWEAKPWHQYY
PIFLTEKRLDAGLAFWKEHAKTIERASKEFNVDPQIIVAIIGIETFYGGYMGNYPVKDALYTLGFHYPPRATFFRSEFAN
LQSLVKEERLDINSLKGSYAGAMGFGQFIPSSYRHYAVDFDNDGSRDLLISTTDAIGSVANYFHQHGWQKNQPVTLQLTL
NKDLPDTVKTWSGEKLHYQVADILSPNVALAESIDLDVSQPALVVKLEQAEHDEYWLGLKNFYVITRYNRSPLYAMAVYQ
FSQELKKAYATQ
>Mature_331_residues
TLLKRMLAPLALTLFSCSAAAAPSDSEVEALKAEFIKTQSGKGFDAEDTTIFIKNAKYNQAVIDAMTKPWEAKPWHQYYP
IFLTEKRLDAGLAFWKEHAKTIERASKEFNVDPQIIVAIIGIETFYGGYMGNYPVKDALYTLGFHYPPRATFFRSEFANL
QSLVKEERLDINSLKGSYAGAMGFGQFIPSSYRHYAVDFDNDGSRDLLISTTDAIGSVANYFHQHGWQKNQPVTLQLTLN
KDLPDTVKTWSGEKLHYQVADILSPNVALAESIDLDVSQPALVVKLEQAEHDEYWLGLKNFYVITRYNRSPLYAMAVYQF
SQELKKAYATQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=306, Percent_Identity=38.562091503268, Blast_Score=192, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 37563; Mature: 37432

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLKRMLAPLALTLFSCSAAAAPSDSEVEALKAEFIKTQSGKGFDAEDTTIFIKNAKYN
CHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCC
QAVIDAMTKPWEAKPWHQYYPIFLTEKRLDAGLAFWKEHAKTIERASKEFNVDPQIIVAI
HHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEE
IGIETFYGGYMGNYPVKDALYTLGFHYPPRATFFRSEFANLQSLVKEERLDINSLKGSYA
EHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCHH
GAMGFGQFIPSSYRHYAVDFDNDGSRDLLISTTDAIGSVANYFHQHGWQKNQPVTLQLTL
HHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCEEEEEEE
NKDLPDTVKTWSGEKLHYQVADILSPNVALAESIDLDVSQPALVVKLEQAEHDEYWLGLK
CCCCCHHHHHCCCCEEEEEHHHHCCCCCHHHHCCCCCCCCCEEEEEEECCCCCCEEEECE
NFYVITRYNRSPLYAMAVYQFSQELKKAYATQ
EEEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLLKRMLAPLALTLFSCSAAAAPSDSEVEALKAEFIKTQSGKGFDAEDTTIFIKNAKYN
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCC
QAVIDAMTKPWEAKPWHQYYPIFLTEKRLDAGLAFWKEHAKTIERASKEFNVDPQIIVAI
HHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEE
IGIETFYGGYMGNYPVKDALYTLGFHYPPRATFFRSEFANLQSLVKEERLDINSLKGSYA
EHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCHH
GAMGFGQFIPSSYRHYAVDFDNDGSRDLLISTTDAIGSVANYFHQHGWQKNQPVTLQLTL
HHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCEEEEEEE
NKDLPDTVKTWSGEKLHYQVADILSPNVALAESIDLDVSQPALVVKLEQAEHDEYWLGLK
CCCCCHHHHHCCCCEEEEEHHHHCCCCCHHHHCCCCCCCCCEEEEEEECCCCCCEEEECE
NFYVITRYNRSPLYAMAVYQFSQELKKAYATQ
EEEEEEEECCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]