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The map label for this gene is prs [H]

Identifier: 157962947

GI number: 157962947

Start: 3833116

End: 3834090

Strand: Direct

Name: prs [H]

Synonym: Spea_3130

Alternate gene names: 157962947

Gene position: 3833116-3834090 (Clockwise)

Preceding gene: 157962946

Following gene: 157962953

Centisome position: 74.08

GC content: 45.64

Gene sequence:

>975_bases
ATGAAGCATACGCCTGAGGTTCCTACAGTGCCTGACATTAAACTTTTTGCCGGTAACGCTACACCTAGTCTCGCTAAGAA
GATAGCCGATCGTCTATTTTGTAAACTTGGAGACGCAGAAGTAGGCGTTTTCAGTGACGGCGAAATCAGTGTCCAAATTA
ATGAAAATGTACGTGGTGCGGATGTATTCATCATTCAATCTACTTGCGCTCCTACTAACGATAACCTAATGGAACTTATC
GTTATGGTTGACGCGCTTCGCCGTGCATCAGCTGGTCGTATTACAGCGGTTATTCCTTACTTTGGTTACGCGCGTCAGGA
CCGTCGAGTTCGTAGTGCTCGTGTACCGATTACAGCTAAAGTTGTTGCCGATTTCCTATCAAGCGTTGGTGTTGACCGCG
TATTGACATGTGATCTACACGCAGAGCAAATCCAAGGTTTCTTTGACGTTCCAGTTGATAACGTATTTGGTAGCCCTGTT
CTACTTGAAGACATGTTAGCTAAGAACCTAGAAAACCCTGTTGTTGTTTCTCCTGATATCGGTGGCGTTGTGCGCGCTCG
CGCAGTCGCTAAGCTACTTGATGACTCTGATTTAGCAATTATCGATAAGCGTCGCCCACAAGCGAACGTTGCTCAGGTTA
TGCATATCATTGGTGACGTTCAAGGCCGTGACTGTATCATCGTTGATGATATGATCGACACTGGCGGTACGCTATGTAAA
GCTGCTGAAGCACTTAAAGAGCACGGTGCAAACCGCGTATTCGCTTACGCAACTCACCCTGTTTTCTCTGGCAACGCTGC
TAAGAACATCGCTGAGTCTGTAATCGATGAAGTAATCGTAACCGATACGATTCCATTGAGCCCAGAAATTGCTGCTCTAG
ACAAAGTTTCACAGTTAACTATGTCTACAGTTATGGCTGAAGCTATTCGCCGTGTAAGCAACGAAGAATCAATCTCTGCA
ATGTTCAAGCACTAA

Upstream 100 bases:

>100_bases
CAAGGCTTTGTTGCGAAAGGGGCAAATATATCACCGTTGATGCTGAGATTAGCTCAGTGTTAACCACATTCTGGGATATT
TTTAAATCCCAGCCACAATC

Downstream 100 bases:

>100_bases
TTCATAGCAGACGCTAAAAGTTAGGCTCGTAAAAGAGAGAAAAAACGCACTCCTTGAGTGCGTTTTTTTATGCCTAAACA
ACTCTATTCAATATCTTCGA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKHTPEVPTVPDIKLFAGNATPSLAKKIADRLFCKLGDAEVGVFSDGEISVQINENVRGADVFIIQSTCAPTNDNLMELI
VMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTCDLHAEQIQGFFDVPVDNVFGSPV
LLEDMLAKNLENPVVVSPDIGGVVRARAVAKLLDDSDLAIIDKRRPQANVAQVMHIIGDVQGRDCIIVDDMIDTGGTLCK
AAEALKEHGANRVFAYATHPVFSGNAAKNIAESVIDEVIVTDTIPLSPEIAALDKVSQLTMSTVMAEAIRRVSNEESISA
MFKH

Sequences:

>Translated_324_residues
MKHTPEVPTVPDIKLFAGNATPSLAKKIADRLFCKLGDAEVGVFSDGEISVQINENVRGADVFIIQSTCAPTNDNLMELI
VMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTCDLHAEQIQGFFDVPVDNVFGSPV
LLEDMLAKNLENPVVVSPDIGGVVRARAVAKLLDDSDLAIIDKRRPQANVAQVMHIIGDVQGRDCIIVDDMIDTGGTLCK
AAEALKEHGANRVFAYATHPVFSGNAAKNIAESVIDEVIVTDTIPLSPEIAALDKVSQLTMSTVMAEAIRRVSNEESISA
MFKH
>Mature_324_residues
MKHTPEVPTVPDIKLFAGNATPSLAKKIADRLFCKLGDAEVGVFSDGEISVQINENVRGADVFIIQSTCAPTNDNLMELI
VMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTCDLHAEQIQGFFDVPVDNVFGSPV
LLEDMLAKNLENPVVVSPDIGGVVRARAVAKLLDDSDLAIIDKRRPQANVAQVMHIIGDVQGRDCIIVDDMIDTGGTLCK
AAEALKEHGANRVFAYATHPVFSGNAAKNIAESVIDEVIVTDTIPLSPEIAALDKVSQLTMSTVMAEAIRRVSNEESISA
MFKH

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=318, Percent_Identity=47.4842767295598, Blast_Score=292, Evalue=3e-79,
Organism=Homo sapiens, GI4506129, Length=318, Percent_Identity=46.5408805031447, Blast_Score=289, Evalue=3e-78,
Organism=Homo sapiens, GI84875539, Length=320, Percent_Identity=46.25, Blast_Score=288, Evalue=5e-78,
Organism=Homo sapiens, GI28557709, Length=318, Percent_Identity=46.5408805031447, Blast_Score=285, Evalue=4e-77,
Organism=Homo sapiens, GI4506133, Length=360, Percent_Identity=36.3888888888889, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI194018537, Length=345, Percent_Identity=36.231884057971, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI310128524, Length=145, Percent_Identity=31.7241379310345, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI310115209, Length=145, Percent_Identity=31.7241379310345, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI310118259, Length=145, Percent_Identity=31.7241379310345, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI310119946, Length=145, Percent_Identity=31.7241379310345, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1787458, Length=315, Percent_Identity=83.4920634920635, Blast_Score=547, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI25149168, Length=317, Percent_Identity=45.1104100946372, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17554702, Length=317, Percent_Identity=45.1104100946372, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI71989924, Length=317, Percent_Identity=45.1104100946372, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17554704, Length=312, Percent_Identity=44.8717948717949, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17570245, Length=337, Percent_Identity=34.1246290801187, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6320946, Length=315, Percent_Identity=46.031746031746, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6319403, Length=316, Percent_Identity=45.253164556962, Blast_Score=265, Evalue=8e-72,
Organism=Saccharomyces cerevisiae, GI6321776, Length=314, Percent_Identity=46.8152866242038, Blast_Score=262, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6322667, Length=203, Percent_Identity=41.3793103448276, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6324511, Length=115, Percent_Identity=43.4782608695652, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21355239, Length=318, Percent_Identity=45.5974842767296, Blast_Score=275, Evalue=3e-74,
Organism=Drosophila melanogaster, GI45551540, Length=340, Percent_Identity=42.6470588235294, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24651458, Length=357, Percent_Identity=34.453781512605, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24651456, Length=357, Percent_Identity=34.453781512605, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI281362873, Length=357, Percent_Identity=34.453781512605, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24651454, Length=357, Percent_Identity=34.453781512605, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24651462, Length=184, Percent_Identity=37.5, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24651464, Length=184, Percent_Identity=37.5, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI45552010, Length=184, Percent_Identity=37.5, Blast_Score=127, Evalue=9e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34948; Mature: 34948

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHTPEVPTVPDIKLFAGNATPSLAKKIADRLFCKLGDAEVGVFSDGEISVQINENVRGA
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCCC
DVFIIQSTCAPTNDNLMELIVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAK
EEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHH
VVADFLSSVGVDRVLTCDLHAEQIQGFFDVPVDNVFGSPVLLEDMLAKNLENPVVVSPDI
HHHHHHHHCCCCEEEEECCCHHHCCCEECCCHHHHCCCHHHHHHHHHHCCCCCEEECCCC
GGVVRARAVAKLLDDSDLAIIDKRRPQANVAQVMHIIGDVQGRDCIIVDDMIDTGGTLCK
CHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHH
AAEALKEHGANRVFAYATHPVFSGNAAKNIAESVIDEVIVTDTIPLSPEIAALDKVSQLT
HHHHHHHCCCCEEEEEEECCEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
MSTVMAEAIRRVSNEESISAMFKH
HHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MKHTPEVPTVPDIKLFAGNATPSLAKKIADRLFCKLGDAEVGVFSDGEISVQINENVRGA
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCCC
DVFIIQSTCAPTNDNLMELIVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAK
EEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHH
VVADFLSSVGVDRVLTCDLHAEQIQGFFDVPVDNVFGSPVLLEDMLAKNLENPVVVSPDI
HHHHHHHHCCCCEEEEECCCHHHCCCEECCCHHHHCCCHHHHHHHHHHCCCCCEEECCCC
GGVVRARAVAKLLDDSDLAIIDKRRPQANVAQVMHIIGDVQGRDCIIVDDMIDTGGTLCK
CHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHH
AAEALKEHGANRVFAYATHPVFSGNAAKNIAESVIDEVIVTDTIPLSPEIAALDKVSQLT
HHHHHHHCCCCEEEEEEECCEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
MSTVMAEAIRRVSNEESISAMFKH
HHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]