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The map label for this gene is glcA [C]

Identifier: 157962812

GI number: 157962812

Start: 3666988

End: 3668631

Strand: Direct

Name: glcA [C]

Synonym: Spea_2994

Alternate gene names: 157962812

Gene position: 3666988-3668631 (Clockwise)

Preceding gene: 157962811

Following gene: 157962813

Centisome position: 70.87

GC content: 48.72

Gene sequence:

>1644_bases
ATGACCTTTATTCAACTTCTCGCAAGTTTAACGCCGATTATCAGCGTTATGCTGTTTTTAGTCCTACTCAGGATGCCTGC
ATCCAGAGCCATGCCAATTTCTATGGTGTTCACCGGCCTCGCTGCAGTATTCGTTTGGCAGATGGACACCACCTTCTTAG
CCGCCTCAGTGGTTGAAGGATTACTTTCGGCCCTAACTCCACTGACGATTATCTTTGGCGCAGTATTCTTGCTTAACACG
CTTAAATACTCAGGAGCTATGGATACCATTCGCGCAGGTTTCACCAACATCAGCGGGGATGCTCGAGTTCAGGTCATCAT
CATATGTTGGTTGTTTGGCTCATTTATCGAAGGTTCAGCTGGTTTTGGTACGCCTGCAGCGATTGGTGCACCATTACTGG
TATTATTGGGAATTCCACCTATTGCTGCTGCGGTTGTCGCCTTGATTGCCGACTCGACCTCGGTATCGTTTGGTGCTATC
GGCCTGCCTGTTCTGTTTGGTATGGAGCAAGGGCTGACACAAGGTGGCAGCAATATGGCCGCAGAACAAATCGCTCAACA
TGGCGGTAGTTTTGCCGAGTACGCGCAGTTTATTGCCATGCACATGATCACTATCGATTTAATCACTGGCACGCTGATCC
CATTGACTATGGTGGCAATACTTACAGGCTTCTTCGGGCGTAATAAATCCTTTAAAGAAGGTTTAGCCATCTGGAAATTC
GCTCTATTTGCAGGCCTTGCTTTCACCGTTCCCGCCTGGTTAATCAACTACTTTGCCGGTCCTGAATTCCCATCAGTGAT
TGGTGCACTTGTGGGTATGGCGCTAGTAATTCCAGTCGCCAAGAAAGGCTATCTATTACCAAAAGAGCAATGGAATGATT
TTGCGGAAAATGACGGCCAAGAGCGTAAAGAGTTGGCCACCGAGGTAAAGTTTTCTCAACTTGCAGCATGGTCGCCTTAC
TTGATTATGGCTACGCTACTGGTACTTTCTCGTACCATCGCGCCGCTTAAAACCTGGTTATCTAGCTTTAACATCAGCTG
GACAGGCTTGCTAGGCACAGAGCTCAAAGCAGGCTTCGCCACACTTTATGCGCCAGGCGCCTTCTTCGTCTTAGTCTGTA
TCCTTGGCTTCTTCCTATTTAAGATGAAGTCTCCCGCGATAAAAGAGTCCATCTCAGTCTCTTGTAAGTCTATGGTGCCA
ACTATTATCTCCCTTGGCGCATCGGTACCTATGGTGAAAATCTTCCTTAACTCTGGTGAAAATGCATCGGGGCTTGCCTC
TATGCCTGTCGCTCTGGCCGATACGCTGGCTAACAGCATGGGCGCAGTATGGGCTTGGGTATCACCGATTGTCGGTATAT
TTGGCGCTTTTCTATCGGGGTCTGCAACCTTCTCCAACATGATGTTCTCAGGTCTGCAATACAGTGTTGCCGATAATATT
GGTGCCAACCACGCGCTAATTTTAGCCATGCAAGGCATTGGCGCTAACGCCGGTAACATGATGTGTGTAATGAATGTAGT
GGCTGCGGCTACGGTTGTGGGTATGGCGGGACGCGAATCAGAGATCATCCGTAAGACGATGCCTGTTGCACTAGGCTATG
CTTTAGTCGCAGGAACTATCGCCGCGGTTTGGGGTGGTTTCTAA

Upstream 100 bases:

>100_bases
CAAAAATTGAAGTTAACCGCTCAAAATTAGCCATTCAAATCTAGTCATTTGAATTCGAAATGGAAGTTAGATACAAAAAT
ATAAATCAAACGAGGCAAGC

Downstream 100 bases:

>100_bases
CACCTGTTAAACCTGATTAGCAAGCATAACCTAACAGAATAATCATATCGTGAAAGCCGCAGAAACTGCGGCTTTATTCA
CTCAAAATAAGTGTGTGAGA

Product: L-lactate permease

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MTFIQLLASLTPIISVMLFLVLLRMPASRAMPISMVFTGLAAVFVWQMDTTFLAASVVEGLLSALTPLTIIFGAVFLLNT
LKYSGAMDTIRAGFTNISGDARVQVIIICWLFGSFIEGSAGFGTPAAIGAPLLVLLGIPPIAAAVVALIADSTSVSFGAI
GLPVLFGMEQGLTQGGSNMAAEQIAQHGGSFAEYAQFIAMHMITIDLITGTLIPLTMVAILTGFFGRNKSFKEGLAIWKF
ALFAGLAFTVPAWLINYFAGPEFPSVIGALVGMALVIPVAKKGYLLPKEQWNDFAENDGQERKELATEVKFSQLAAWSPY
LIMATLLVLSRTIAPLKTWLSSFNISWTGLLGTELKAGFATLYAPGAFFVLVCILGFFLFKMKSPAIKESISVSCKSMVP
TIISLGASVPMVKIFLNSGENASGLASMPVALADTLANSMGAVWAWVSPIVGIFGAFLSGSATFSNMMFSGLQYSVADNI
GANHALILAMQGIGANAGNMMCVMNVVAAATVVGMAGRESEIIRKTMPVALGYALVAGTIAAVWGGF

Sequences:

>Translated_547_residues
MTFIQLLASLTPIISVMLFLVLLRMPASRAMPISMVFTGLAAVFVWQMDTTFLAASVVEGLLSALTPLTIIFGAVFLLNT
LKYSGAMDTIRAGFTNISGDARVQVIIICWLFGSFIEGSAGFGTPAAIGAPLLVLLGIPPIAAAVVALIADSTSVSFGAI
GLPVLFGMEQGLTQGGSNMAAEQIAQHGGSFAEYAQFIAMHMITIDLITGTLIPLTMVAILTGFFGRNKSFKEGLAIWKF
ALFAGLAFTVPAWLINYFAGPEFPSVIGALVGMALVIPVAKKGYLLPKEQWNDFAENDGQERKELATEVKFSQLAAWSPY
LIMATLLVLSRTIAPLKTWLSSFNISWTGLLGTELKAGFATLYAPGAFFVLVCILGFFLFKMKSPAIKESISVSCKSMVP
TIISLGASVPMVKIFLNSGENASGLASMPVALADTLANSMGAVWAWVSPIVGIFGAFLSGSATFSNMMFSGLQYSVADNI
GANHALILAMQGIGANAGNMMCVMNVVAAATVVGMAGRESEIIRKTMPVALGYALVAGTIAAVWGGF
>Mature_546_residues
TFIQLLASLTPIISVMLFLVLLRMPASRAMPISMVFTGLAAVFVWQMDTTFLAASVVEGLLSALTPLTIIFGAVFLLNTL
KYSGAMDTIRAGFTNISGDARVQVIIICWLFGSFIEGSAGFGTPAAIGAPLLVLLGIPPIAAAVVALIADSTSVSFGAIG
LPVLFGMEQGLTQGGSNMAAEQIAQHGGSFAEYAQFIAMHMITIDLITGTLIPLTMVAILTGFFGRNKSFKEGLAIWKFA
LFAGLAFTVPAWLINYFAGPEFPSVIGALVGMALVIPVAKKGYLLPKEQWNDFAENDGQERKELATEVKFSQLAAWSPYL
IMATLLVLSRTIAPLKTWLSSFNISWTGLLGTELKAGFATLYAPGAFFVLVCILGFFLFKMKSPAIKESISVSCKSMVPT
IISLGASVPMVKIFLNSGENASGLASMPVALADTLANSMGAVWAWVSPIVGIFGAFLSGSATFSNMMFSGLQYSVADNIG
ANHALILAMQGIGANAGNMMCVMNVVAAATVVGMAGRESEIIRKTMPVALGYALVAGTIAAVWGGF

Specific function: May play a role in L-lactate transport [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=575, Percent_Identity=29.9130434782609, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1790031, Length=560, Percent_Identity=27.5, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 57557; Mature: 57426

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCC
>Mature Secondary Structure 
TFIQLLASLTPIISVMLFLVLLRMPASRAMPISMVFTGLAAVFVWQMDTTFLAASVVEG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]