Click here to switch to the map view.

The map label for this gene is ppnK [H]

Identifier: 157962811

GI number: 157962811

Start: 3665400

End: 3666278

Strand: Direct

Name: ppnK [H]

Synonym: Spea_2993

Alternate gene names: 157962811

Gene position: 3665400-3666278 (Clockwise)

Preceding gene: 157962809

Following gene: 157962812

Centisome position: 70.83

GC content: 46.87

Gene sequence:

>879_bases
ATGAGCAATACGTTTCACACAATTGGTCTAATTGGTAAGCCTAACCATAAAGGTACCACCCAGACCTTAAAAAGATTGCA
TCATTGGCTTAGTATGCAAGGCTACAAAGTCTTAGTCGAAGAGCGAGTAGCCGGTGAACTTGGCCCTCAAGCTCAATCTG
TTGACCTGTTAGAGATAGGTAAGCAATGTGATTTAGCCATCGTCGTCGGTGGTGACGGCAACATGCTAGGTGCCGCTCGC
GTGCTTGCGAGGTTCAATATAGGTGTTATCGGGGTTAACCGTGGCAACTTAGGCTTCTTGACCGACCTGCCACCAGACTC
ATTCGAAGAAACATTATCAAAAGTACTCGAAGGCGAGTTTGAAATAGAACAGCGGTTCTTACTCGAAGCCGAAGTACACC
GGCATGGTGAATTAAAGTCCAGCAACACAGCGGTTAACGAAGCGGTACTTCATCCTGGCAAGATTGCGCATATGATCGAA
TTTGAAGTCTATATCGACGACAAATTCATGTATAGCCAGCGCGCAGACGGCATGATTATCTCGACGCCTACTGGATCGAC
GGCCTATTCACTCTCTGCTGGCGGCGCGATTTTAACTCCTAACTTGTCTGCGATGATCTTAGTACCCATGTTCCCCCATA
CGCTTTCCTGCAGACCAATCGTTGTCGACGCTGCCAGTATCATCAAGTTAGTCGTATCACCACATAACGCCGATAATTTA
GAGGTCAGCTGTGATGGCCACGTCCACCTGTCTGTTTTGCCTGGTGATGAAATTATAATCAAACGTAGCGACGAAACCTT
AAGACTTGTTCACCCTAAGGGGCACAACTATTTTCACGTTTTACGTACTAAACTTGGCTGGGGCAGCAAACTGTTCTAA

Upstream 100 bases:

>100_bases
TTTTGCTCCAGCTAAAAATGCTTTGTTTCTGCATGCTCTGAGCATATTATGGGGATCAAATTTAAGGTTTCAAGGCCTAA
AGCTGGATCTAACATAATTT

Downstream 100 bases:

>100_bases
GTTCTTAAGTTCTAAGTGCCTAAGCTCTAATCCTTAAATATTGCCGATATGCCAAACTAGAGGCTATCGGCAACACTATC
GCCCGCTTTACTCTCTCGTA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSNTFHTIGLIGKPNHKGTTQTLKRLHHWLSMQGYKVLVEERVAGELGPQAQSVDLLEIGKQCDLAIVVGGDGNMLGAAR
VLARFNIGVIGVNRGNLGFLTDLPPDSFEETLSKVLEGEFEIEQRFLLEAEVHRHGELKSSNTAVNEAVLHPGKIAHMIE
FEVYIDDKFMYSQRADGMIISTPTGSTAYSLSAGGAILTPNLSAMILVPMFPHTLSCRPIVVDAASIIKLVVSPHNADNL
EVSCDGHVHLSVLPGDEIIIKRSDETLRLVHPKGHNYFHVLRTKLGWGSKLF

Sequences:

>Translated_292_residues
MSNTFHTIGLIGKPNHKGTTQTLKRLHHWLSMQGYKVLVEERVAGELGPQAQSVDLLEIGKQCDLAIVVGGDGNMLGAAR
VLARFNIGVIGVNRGNLGFLTDLPPDSFEETLSKVLEGEFEIEQRFLLEAEVHRHGELKSSNTAVNEAVLHPGKIAHMIE
FEVYIDDKFMYSQRADGMIISTPTGSTAYSLSAGGAILTPNLSAMILVPMFPHTLSCRPIVVDAASIIKLVVSPHNADNL
EVSCDGHVHLSVLPGDEIIIKRSDETLRLVHPKGHNYFHVLRTKLGWGSKLF
>Mature_291_residues
SNTFHTIGLIGKPNHKGTTQTLKRLHHWLSMQGYKVLVEERVAGELGPQAQSVDLLEIGKQCDLAIVVGGDGNMLGAARV
LARFNIGVIGVNRGNLGFLTDLPPDSFEETLSKVLEGEFEIEQRFLLEAEVHRHGELKSSNTAVNEAVLHPGKIAHMIEF
EVYIDDKFMYSQRADGMIISTPTGSTAYSLSAGGAILTPNLSAMILVPMFPHTLSCRPIVVDAASIIKLVVSPHNADNLE
VSCDGHVHLSVLPGDEIIIKRSDETLRLVHPKGHNYFHVLRTKLGWGSKLF

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Homo sapiens, GI55743112, Length=266, Percent_Identity=31.203007518797, Blast_Score=104, Evalue=7e-23,
Organism=Escherichia coli, GI1788968, Length=293, Percent_Identity=57.3378839590444, Blast_Score=349, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6320794, Length=242, Percent_Identity=30.1652892561983, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6322509, Length=240, Percent_Identity=30.4166666666667, Blast_Score=119, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6325068, Length=215, Percent_Identity=32.5581395348837, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28573828, Length=253, Percent_Identity=30.8300395256917, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28573830, Length=253, Percent_Identity=30.8300395256917, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28573826, Length=253, Percent_Identity=30.8300395256917, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161077047, Length=253, Percent_Identity=30.8300395256917, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI28573832, Length=253, Percent_Identity=30.8300395256917, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24653422, Length=249, Percent_Identity=28.1124497991968, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI20129957, Length=249, Percent_Identity=28.1124497991968, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281363323, Length=249, Percent_Identity=28.1124497991968, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281363321, Length=249, Percent_Identity=28.1124497991968, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24653424, Length=249, Percent_Identity=28.1124497991968, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 31956; Mature: 31825

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTFHTIGLIGKPNHKGTTQTLKRLHHWLSMQGYKVLVEERVAGELGPQAQSVDLLEIG
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCCCCHHHHCC
KQCDLAIVVGGDGNMLGAARVLARFNIGVIGVNRGNLGFLTDLPPDSFEETLSKVLEGEF
CCCCEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCC
EIEQRFLLEAEVHRHGELKSSNTAVNEAVLHPGKIAHMIEFEVYIDDKFMYSQRADGMII
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEEEEEEEECCEEEECCCCCCEEE
STPTGSTAYSLSAGGAILTPNLSAMILVPMFPHTLSCRPIVVDAASIIKLVVSPHNADNL
ECCCCCCEEEEECCCEEECCCCCEEEEEECCCCCCCCCEEEEEHHHHHEEEECCCCCCCE
EVSCDGHVHLSVLPGDEIIIKRSDETLRLVHPKGHNYFHVLRTKLGWGSKLF
EEEECCEEEEEEECCCEEEEEECCCEEEEECCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure 
SNTFHTIGLIGKPNHKGTTQTLKRLHHWLSMQGYKVLVEERVAGELGPQAQSVDLLEIG
CCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCCCCHHHHCC
KQCDLAIVVGGDGNMLGAARVLARFNIGVIGVNRGNLGFLTDLPPDSFEETLSKVLEGEF
CCCCEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCC
EIEQRFLLEAEVHRHGELKSSNTAVNEAVLHPGKIAHMIEFEVYIDDKFMYSQRADGMII
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEEEEEEEECCEEEECCCCCCEEE
STPTGSTAYSLSAGGAILTPNLSAMILVPMFPHTLSCRPIVVDAASIIKLVVSPHNADNL
ECCCCCCEEEEECCCEEECCCCCEEEEEECCCCCCCCCEEEEEHHHHHEEEECCCCCCCE
EVSCDGHVHLSVLPGDEIIIKRSDETLRLVHPKGHNYFHVLRTKLGWGSKLF
EEEECCEEEEEEECCCEEEEEECCCEEEEECCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA