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The map label for this gene is mvaB [H]

Identifier: 157962622

GI number: 157962622

Start: 3422068

End: 3422973

Strand: Direct

Name: mvaB [H]

Synonym: Spea_2802

Alternate gene names: 157962622

Gene position: 3422068-3422973 (Clockwise)

Preceding gene: 157962621

Following gene: 157962623

Centisome position: 66.13

GC content: 48.9

Gene sequence:

>906_bases
ATGAAAATGACAAGCCCTTATCCTAAAAAGGTTAGCATTTTTGAAGTCGGTGCCCGTGACGGCCTGCAGAACGAAAAGCC
TGTCACCACCCAAGATAAAATTATCTTGATTGAAGACTTAGCAGCCGCAGGCATAAAACGTATCGAAGCCGCTAGCTTTG
TGTCACCAAAATGGGTGCCGCAGATGGCAGATTCAGCCGATGTGCTTCACGGTATTACTCGTAAAACGGGTGTGACCTAC
AGCGCTTTAACGCCTAACTTAAAAGGCTTGGAACTTGCTCTTGACGCTGGCGCCGATGAAGTTGCTATCTTTGGCGCAGC
TTCAGAGAGCTTTAGTCAGAAGAATATTAACTGCTCGATAGAAGAGTCCATTGAGCGCTTTATCCCAGTTATGGAGCAGG
CTAAGGCCAGAAACATCCCTGTTCGTGGTTATGTTTCTTGCACTTTGGGTTGCCCTTACGAAGGAGACATCGCGGTCAGC
GAAGTGGCTCGGGTGTCTGAATTACTTTACAAAATGGGCTGCTACGAGATCTCCCTTGGCGACACCATAGGCGTTGGCAC
ACCGCTTAATGCCCGTAGAATGATTGAAGCGGTAGCAGAGCTTGTGCCCGTAGAAAAACTGGCACTGCATTTTCATGACA
CTTATGGCCAAGCGCTCGCTAATATTCTGGCTTGCCTAGAAACCGGTGTCAGCGTTATCGACTCGTCAGTTGCAGGTCTT
GGAGGCTGCCCCTATGCCAAAGGCGCATCGGGTAATTTGGCCACAGAAGACTTAGTTTATATGCTGCATGGACTTGGTAT
CGAAACTGGTATCGACTTAACTAAACTGGTTAATGCTGGCAATAAGATCAGCCAAGCCCTAGGCCGTCCGTCGGGTTCAA
AGGTGGCTAGAGCCCTCAGCGAATAA

Upstream 100 bases:

>100_bases
TCGAGGCCGGTGAACTGGTTAGTGACGGTATGTTACTTGCCGAGGTCGTGGCTGAAGTTGAGGCGAAAGCTGAAATGCCG
ACAGAAAAGGAAGAAGCAGG

Downstream 100 bases:

>100_bases
AGAGCCCTCAGCGAATAAAGAGCCCTCAGCGAATAAATAGCGCTAAGTTAATAAAGAGCGATTAGCAAATAAGAAATAGA
CTCTGAACCTAGGACCTAGG

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKMTSPYPKKVSIFEVGARDGLQNEKPVTTQDKIILIEDLAAAGIKRIEAASFVSPKWVPQMADSADVLHGITRKTGVTY
SALTPNLKGLELALDAGADEVAIFGAASESFSQKNINCSIEESIERFIPVMEQAKARNIPVRGYVSCTLGCPYEGDIAVS
EVARVSELLYKMGCYEISLGDTIGVGTPLNARRMIEAVAELVPVEKLALHFHDTYGQALANILACLETGVSVIDSSVAGL
GGCPYAKGASGNLATEDLVYMLHGLGIETGIDLTKLVNAGNKISQALGRPSGSKVARALSE

Sequences:

>Translated_301_residues
MKMTSPYPKKVSIFEVGARDGLQNEKPVTTQDKIILIEDLAAAGIKRIEAASFVSPKWVPQMADSADVLHGITRKTGVTY
SALTPNLKGLELALDAGADEVAIFGAASESFSQKNINCSIEESIERFIPVMEQAKARNIPVRGYVSCTLGCPYEGDIAVS
EVARVSELLYKMGCYEISLGDTIGVGTPLNARRMIEAVAELVPVEKLALHFHDTYGQALANILACLETGVSVIDSSVAGL
GGCPYAKGASGNLATEDLVYMLHGLGIETGIDLTKLVNAGNKISQALGRPSGSKVARALSE
>Mature_301_residues
MKMTSPYPKKVSIFEVGARDGLQNEKPVTTQDKIILIEDLAAAGIKRIEAASFVSPKWVPQMADSADVLHGITRKTGVTY
SALTPNLKGLELALDAGADEVAIFGAASESFSQKNINCSIEESIERFIPVMEQAKARNIPVRGYVSCTLGCPYEGDIAVS
EVARVSELLYKMGCYEISLGDTIGVGTPLNARRMIEAVAELVPVEKLALHFHDTYGQALANILACLETGVSVIDSSVAGL
GGCPYAKGASGNLATEDLVYMLHGLGIETGIDLTKLVNAGNKISQALGRPSGSKVARALSE

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=292, Percent_Identity=65.4109589041096, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI109150422, Length=292, Percent_Identity=59.9315068493151, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI109150427, Length=292, Percent_Identity=59.9315068493151, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI260654708, Length=136, Percent_Identity=69.1176470588235, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17510563, Length=292, Percent_Identity=52.3972602739726, Blast_Score=308, Evalue=3e-84,
Organism=Drosophila melanogaster, GI24582381, Length=290, Percent_Identity=61.7241379310345, Blast_Score=370, Evalue=1e-103,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000138
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 31860; Mature: 31860

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS01062 HMG_COA_LYASE ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMTSPYPKKVSIFEVGARDGLQNEKPVTTQDKIILIEDLAAAGIKRIEAASFVSPKWVP
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCH
QMADSADVLHGITRKTGVTYSALTPNLKGLELALDAGADEVAIFGAASESFSQKNINCSI
HHCCHHHHHHHHHHHCCCEEHHCCCCCCCEEEEEECCCCCEEEEECCCHHHHCCCCCCCH
EESIERFIPVMEQAKARNIPVRGYVSCTLGCPYEGDIAVSEVARVSELLYKMGCYEISLG
HHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECC
DTIGVGTPLNARRMIEAVAELVPVEKLALHFHDTYGQALANILACLETGVSVIDSSVAGL
CEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GGCPYAKGASGNLATEDLVYMLHGLGIETGIDLTKLVNAGNKISQALGRPSGSKVARALS
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHCCCCHHHHHHHHC
E
C
>Mature Secondary Structure
MKMTSPYPKKVSIFEVGARDGLQNEKPVTTQDKIILIEDLAAAGIKRIEAASFVSPKWVP
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCH
QMADSADVLHGITRKTGVTYSALTPNLKGLELALDAGADEVAIFGAASESFSQKNINCSI
HHCCHHHHHHHHHHHCCCEEHHCCCCCCCEEEEEECCCCCEEEEECCCHHHHCCCCCCCH
EESIERFIPVMEQAKARNIPVRGYVSCTLGCPYEGDIAVSEVARVSELLYKMGCYEISLG
HHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECC
DTIGVGTPLNARRMIEAVAELVPVEKLALHFHDTYGQALANILACLETGVSVIDSSVAGL
CEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GGCPYAKGASGNLATEDLVYMLHGLGIETGIDLTKLVNAGNKISQALGRPSGSKVARALS
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHCCCCHHHHHHHHC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2687236; 2656635; 1637819 [H]