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The map label for this gene is rnhA [H]

Identifier: 157962578

GI number: 157962578

Start: 3370138

End: 3370902

Strand: Direct

Name: rnhA [H]

Synonym: Spea_2757

Alternate gene names: 157962578

Gene position: 3370138-3370902 (Clockwise)

Preceding gene: 157962577

Following gene: 157962579

Centisome position: 65.13

GC content: 46.41

Gene sequence:

>765_bases
ATGGCTAAAAAGTATTACGTGGTATGGGCTGGTCGTGAGACCGGCATTTTTACGAGCTGGGATTACACCAAAAAGCAGGT
CGACAAATTTCCTCAGGCCAAATATAAGTCTTTCCCAACCGAAGCTGAAGCCAAGGCTGCCTTTGCTAAGTCGCCGAGTT
ACGGCAGCGCGACAAGAAAACCTACATCAGGGCCAGCCAAAGCCAAAACAGCCGCTAAAGCCAGCCTAGATATCGATGTT
AGTCGCTGTGATGTATCTATTTTTACCGATGGCGGCTGTGAGCCTAACCCAGGTAAAGCAGGCTCTGGAGTGGCGGTTTA
TCATAATGCCGTGCTTTCAGAACTTTATTACGGCCTTTATAACGCTAACGGCACCAATAACTCAGCCGAGCTAAACGCCT
TACACCAGGCGCTATTGATTGCCGATAAGGCACTAAAACAAGGGCAAAGCGTACAGATCTTAAGTGACTCACAGTACTCA
ATCAACTGCATTACTAATTGGGCTTACGGCTGGAAAACCAAAGGCTGGAAGCGCAAGGTTGCTGGTGATATCAAAAACTT
AGATATTATTCAGCAGTCTCATGCCTTGTATGATTCTCTAAAGGATAAACTTAACATTCAACACGTTGCCGCTCACGTTG
GTATAGAAGGTAACGAATTGGCCGACCGCATGTCGATTTATGCTATCGACCAAAAAGACCCGGCGTTTTGTCGTTATCCA
GACCCTATTGTACTGACTGATATTCTTAGCCTACGTGCCGGTTAG

Upstream 100 bases:

>100_bases
TGCTTGCTCAGTGCAAGAAAAGCCAATAACCATCTTCAAAGGCTACCTCATGTTAACCGTAGCCAAACAATGATTTATTA
AGATTTAGGACATAAGCTAC

Downstream 100 bases:

>100_bases
TATGTAGACATTAGTCGGTGTTTTATATTTATTAAGGCTAATTAATAGGTTTAAAGCATCGACTATGCGACAATCGCCGC
CATGAAAATCTACGTTCACC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MAKKYYVVWAGRETGIFTSWDYTKKQVDKFPQAKYKSFPTEAEAKAAFAKSPSYGSATRKPTSGPAKAKTAAKASLDIDV
SRCDVSIFTDGGCEPNPGKAGSGVAVYHNAVLSELYYGLYNANGTNNSAELNALHQALLIADKALKQGQSVQILSDSQYS
INCITNWAYGWKTKGWKRKVAGDIKNLDIIQQSHALYDSLKDKLNIQHVAAHVGIEGNELADRMSIYAIDQKDPAFCRYP
DPIVLTDILSLRAG

Sequences:

>Translated_254_residues
MAKKYYVVWAGRETGIFTSWDYTKKQVDKFPQAKYKSFPTEAEAKAAFAKSPSYGSATRKPTSGPAKAKTAAKASLDIDV
SRCDVSIFTDGGCEPNPGKAGSGVAVYHNAVLSELYYGLYNANGTNNSAELNALHQALLIADKALKQGQSVQILSDSQYS
INCITNWAYGWKTKGWKRKVAGDIKNLDIIQQSHALYDSLKDKLNIQHVAAHVGIEGNELADRMSIYAIDQKDPAFCRYP
DPIVLTDILSLRAG
>Mature_253_residues
AKKYYVVWAGRETGIFTSWDYTKKQVDKFPQAKYKSFPTEAEAKAAFAKSPSYGSATRKPTSGPAKAKTAAKASLDIDVS
RCDVSIFTDGGCEPNPGKAGSGVAVYHNAVLSELYYGLYNANGTNNSAELNALHQALLIADKALKQGQSVQILSDSQYSI
NCITNWAYGWKTKGWKRKVAGDIKNLDIIQQSHALYDSLKDKLNIQHVAAHVGIEGNELADRMSIYAIDQKDPAFCRYPD
PIVLTDILSLRAG

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=270, Percent_Identity=31.1111111111111, Blast_Score=101, Evalue=8e-22,
Organism=Escherichia coli, GI1786408, Length=141, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115532542, Length=242, Percent_Identity=33.0578512396694, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI45552509, Length=290, Percent_Identity=28.6206896551724, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17137476, Length=144, Percent_Identity=35.4166666666667, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 27779; Mature: 27647

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKYYVVWAGRETGIFTSWDYTKKQVDKFPQAKYKSFPTEAEAKAAFAKSPSYGSATRK
CCCEEEEEEECCCCCEEECCCHHHHHHHHCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCC
PTSGPAKAKTAAKASLDIDVSRCDVSIFTDGGCEPNPGKAGSGVAVYHNAVLSELYYGLY
CCCCCCHHHHHHHCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
NANGTNNSAELNALHQALLIADKALKQGQSVQILSDSQYSINCITNWAYGWKTKGWKRKV
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCCCCCCCCHHHH
AGDIKNLDIIQQSHALYDSLKDKLNIQHVAAHVGIEGNELADRMSIYAIDQKDPAFCRYP
HCCCCCHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCHHHHCCEEEEEECCCCCCCCCCC
DPIVLTDILSLRAG
CCHHHHHHHHHCCC
>Mature Secondary Structure 
AKKYYVVWAGRETGIFTSWDYTKKQVDKFPQAKYKSFPTEAEAKAAFAKSPSYGSATRK
CCEEEEEEECCCCCEEECCCHHHHHHHHCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCC
PTSGPAKAKTAAKASLDIDVSRCDVSIFTDGGCEPNPGKAGSGVAVYHNAVLSELYYGLY
CCCCCCHHHHHHHCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
NANGTNNSAELNALHQALLIADKALKQGQSVQILSDSQYSINCITNWAYGWKTKGWKRKV
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCCCCCCCCHHHH
AGDIKNLDIIQQSHALYDSLKDKLNIQHVAAHVGIEGNELADRMSIYAIDQKDPAFCRYP
HCCCCCHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCHHHHCCEEEEEECCCCCCCCCCC
DPIVLTDILSLRAG
CCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA