Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 157962460

GI number: 157962460

Start: 3216799

End: 3217689

Strand: Direct

Name: gcvA [H]

Synonym: Spea_2639

Alternate gene names: 157962460

Gene position: 3216799-3217689 (Clockwise)

Preceding gene: 157962457

Following gene: 157962467

Centisome position: 62.17

GC content: 45.23

Gene sequence:

>891_bases
ATGTACTCACACCTTCCGCCATTAAATGCACTTAGAGCCTTTGAAGCATCGGCTAGGCTGATGAGCTTTACCCTTGCTGG
TAAAGAACTCTTTGTCACCCATGGAGCCATCAGCAAGCAGGTAAAGTTATTGGAAGAGTTCGTGGGTATGCCGCTGTTTA
TTAGGCAACATCGTAGCCTTAAACTCACCGATGAGGGCGAGCGCTACTTTATTAATATCAAAGCAGCGCTGCATACGATT
AACAACGCCACTAGCGACTTGGTCACTCAACCTCTGAGGACTCAAACCTTGGCGATTAATGTATTACCCAGCTTAACGAT
TAGCTGGCTAATACCTAGAATGGAAGAGTTTAAGTCGCGCTATCCTCATCTGTATGTCGACTTATCCATTGGTGACTTTG
AAGTTGACTTTAACAGTGTTAATTACGATATCGCCATTCGAAGCAGTACCACAGTGCCAAAAGCGGCCAATGTAATAACC
TTAATGGATGAGGATCTCTGTCTGGTGTGCTCGCCTGATCTCGCCTGTAATCTTAAAAACCTTGAAGACGTAAACACCAT
GACCTTATTACAGCACACGACAAGGCCGGGGCTATGGCAGTTATGGGCCAAGAGTGTTGGGCTTGAGCTAACGACGGAAA
AAAAGTTTGGTGTAGAGCATTTTTATATGCTCAGCCAGGCGGCTGTAAGTGGTATGGGCGTAGCACTGATCCCGCGCTTC
TTTATTGAGGATGAGCTTAAAATGGGCAGACTGGTGATCCCATTCAATGCAGATTTCACCAGCCCTTATCGCTATTACTT
ATTGACACCAAAATCGAGTAACCTGCCGTTAAAAGTGCAATCTTTTATCGACTGGCTACTCGAACTATTTAGCCCATATA
GGCAGCAATAG

Upstream 100 bases:

>100_bases
AAGTGTCGCTATTAGACGTTGTTTGCAGTTAAAACTCAAAGGAGTTATTGTTCGCCATGAGTGAGCTTTTCTCACGTAAG
AACAACTTAAGGTTAAACCA

Downstream 100 bases:

>100_bases
CGTTAGTGATGACTAACTCTCTGCTTAAGCCACATTAGATAACGTCGTTTTCGGCTAAATCGAACATCTCTAAGTAGTTC
ATACCTGCATGAACGCTTAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MYSHLPPLNALRAFEASARLMSFTLAGKELFVTHGAISKQVKLLEEFVGMPLFIRQHRSLKLTDEGERYFINIKAALHTI
NNATSDLVTQPLRTQTLAINVLPSLTISWLIPRMEEFKSRYPHLYVDLSIGDFEVDFNSVNYDIAIRSSTTVPKAANVIT
LMDEDLCLVCSPDLACNLKNLEDVNTMTLLQHTTRPGLWQLWAKSVGLELTTEKKFGVEHFYMLSQAAVSGMGVALIPRF
FIEDELKMGRLVIPFNADFTSPYRYYLLTPKSSNLPLKVQSFIDWLLELFSPYRQQ

Sequences:

>Translated_296_residues
MYSHLPPLNALRAFEASARLMSFTLAGKELFVTHGAISKQVKLLEEFVGMPLFIRQHRSLKLTDEGERYFINIKAALHTI
NNATSDLVTQPLRTQTLAINVLPSLTISWLIPRMEEFKSRYPHLYVDLSIGDFEVDFNSVNYDIAIRSSTTVPKAANVIT
LMDEDLCLVCSPDLACNLKNLEDVNTMTLLQHTTRPGLWQLWAKSVGLELTTEKKFGVEHFYMLSQAAVSGMGVALIPRF
FIEDELKMGRLVIPFNADFTSPYRYYLLTPKSSNLPLKVQSFIDWLLELFSPYRQQ
>Mature_296_residues
MYSHLPPLNALRAFEASARLMSFTLAGKELFVTHGAISKQVKLLEEFVGMPLFIRQHRSLKLTDEGERYFINIKAALHTI
NNATSDLVTQPLRTQTLAINVLPSLTISWLIPRMEEFKSRYPHLYVDLSIGDFEVDFNSVNYDIAIRSSTTVPKAANVIT
LMDEDLCLVCSPDLACNLKNLEDVNTMTLLQHTTRPGLWQLWAKSVGLELTTEKKFGVEHFYMLSQAAVSGMGVALIPRF
FIEDELKMGRLVIPFNADFTSPYRYYLLTPKSSNLPLKVQSFIDWLLELFSPYRQQ

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=38.8316151202749, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=30.0341296928328, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=32.1678321678322, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1786401, Length=283, Percent_Identity=27.208480565371, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI145693193, Length=309, Percent_Identity=25.8899676375405, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1789639, Length=307, Percent_Identity=26.0586319218241, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=26.2068965517241, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787128, Length=283, Percent_Identity=24.7349823321555, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790262, Length=258, Percent_Identity=25.968992248062, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI157672245, Length=168, Percent_Identity=31.547619047619, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790208, Length=285, Percent_Identity=23.5087719298246, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081978, Length=260, Percent_Identity=25.3846153846154, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33715; Mature: 33715

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSHLPPLNALRAFEASARLMSFTLAGKELFVTHGAISKQVKLLEEFVGMPLFIRQHRSL
CCCCCCCHHHHHHHHHHHHEEEEEECCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCCE
KLTDEGERYFINIKAALHTINNATSDLVTQPLRTQTLAINVLPSLTISWLIPRMEEFKSR
EEECCCCEEEEEEEHHHHHHCCCHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHH
YPHLYVDLSIGDFEVDFNSVNYDIAIRSSTTVPKAANVITLMDEDLCLVCSPDLACNLKN
CCEEEEEEEECCEEEEECCEEEEEEEECCCCCCCCCCEEEEECCCEEEEECCCCEECCCC
LEDVNTMTLLQHTTRPGLWQLWAKSVGLELTTEKKFGVEHFYMLSQAAVSGMGVALIPRF
CCCCHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHH
FIEDELKMGRLVIPFNADFTSPYRYYLLTPKSSNLPLKVQSFIDWLLELFSPYRQQ
HHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYSHLPPLNALRAFEASARLMSFTLAGKELFVTHGAISKQVKLLEEFVGMPLFIRQHRSL
CCCCCCCHHHHHHHHHHHHEEEEEECCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCCE
KLTDEGERYFINIKAALHTINNATSDLVTQPLRTQTLAINVLPSLTISWLIPRMEEFKSR
EEECCCCEEEEEEEHHHHHHCCCHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHH
YPHLYVDLSIGDFEVDFNSVNYDIAIRSSTTVPKAANVITLMDEDLCLVCSPDLACNLKN
CCEEEEEEEECCEEEEECCEEEEEEEECCCCCCCCCCEEEEECCCEEEEECCCCEECCCC
LEDVNTMTLLQHTTRPGLWQLWAKSVGLELTTEKKFGVEHFYMLSQAAVSGMGVALIPRF
CCCCHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHH
FIEDELKMGRLVIPFNADFTSPYRYYLLTPKSSNLPLKVQSFIDWLLELFSPYRQQ
HHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]