Click here to switch to the map view.

The map label for this gene is nolF [H]

Identifier: 157962431

GI number: 157962431

Start: 3179409

End: 3180497

Strand: Direct

Name: nolF [H]

Synonym: Spea_2610

Alternate gene names: 157962431

Gene position: 3179409-3180497 (Clockwise)

Preceding gene: 157962430

Following gene: 157962432

Centisome position: 61.44

GC content: 46.1

Gene sequence:

>1089_bases
ATGAATAATTCCCAGAAAAACGCCCTGAAAAACACTGCCCTACTTGCCTATCTATTAATAAGCTCTCTACCATTTTTGAG
CCTATCTGCGTCGGTATCGGCTGAGTCTAGAGCGGCTGCTGACAATAAGCATTTAGAATCAATAACAGTAAGCTTCAAAC
CCTATCCCAACTGGGTACAGCTAGATGCAGTCATTGAGCCCACCAAGTCCGCTACCGTATCGGCACAAACCTCCGGTCGC
ATTATCAAGCTCAATTACGATATCAATGATGTGGTTGCAACGGGTGCCCCACTACTTGAGATCACCAGTAAAGAACAAGG
CGCCCAGCTCGCCGCAGCAGAAGCCGAATACGCCAAAGCCCGCGCCGTCAATGTCGAAGCCCAGCAGCAGTTTAAACGCT
ATCAAGCGCTATTTCCTAAGGGAGCCATTTCCAAGGGAGCGATGGATGAAGCCGAAGCTAACGCAAAGTCTGCTCAACAA
GCAGTAAGCGCAGCTAAAGCTAGAATTACTCAAGCCAGCGAATCATTAAATTATACTCTTGTCAGTGCGCCATTTGCAGG
CATAGTCACCCAAAGACATGTTGAAGAGGGCGAAACAGTAAGCCCTGGGCAAGCCTTATACTCAGGTTACTCCTTAAGCC
AGATGCGCGCAGTCACTCAAATCCCCCAGCGTTATGTGGAAGCACTAAAACAACAGCCCAAATTCAAATTAACCTTAGCC
AACGGCCAGCAGATAAACAGTGAGCAACTGACACTATTTACCTTTGTTGATCCAAAGTCTCATAGCTATAAAGTACGCAT
TAACTTACCTGAAAATACAGATGGCCTTATCCCTGGTAGCCTAATTAAAGCCCAATTTGTTAGCGGTAGCCGTAATACCA
TATTCATCCCCGAGTCGTCATTACTCACCATGAATGAACTCAGCGCCGTCTATATTCAGCAAAATGATGCATGGGTACTC
AATCAGGTCAGAACTGGACTCAGCAATAATGGCAAAGTCGAGATCTTAGCAGGATTATCAGAGGGTGATGTAGTTGCTAA
AGATGCCTACCACGCACTGTTACTGCTTAAGCAAAGCAATAAACCATGA

Upstream 100 bases:

>100_bases
TCAAACACAGTCCATCGCTTTTCTCTAATAAATAATTATTGCACATAAAAACCACTTCCATTAGAATTATTGCATGGACT
GGTTTTGATAAGACAATTAA

Downstream 100 bases:

>100_bases
AACCTAATCGAATCTAGTTTTAAGGAAGACCTGTCATGCAAACTCAAAACAAATTAGGTATTTCTGGACGTATTGCTAGC
GCCTTTCAATCCAGTGCTAT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MNNSQKNALKNTALLAYLLISSLPFLSLSASVSAESRAAADNKHLESITVSFKPYPNWVQLDAVIEPTKSATVSAQTSGR
IIKLNYDINDVVATGAPLLEITSKEQGAQLAAAEAEYAKARAVNVEAQQQFKRYQALFPKGAISKGAMDEAEANAKSAQQ
AVSAAKARITQASESLNYTLVSAPFAGIVTQRHVEEGETVSPGQALYSGYSLSQMRAVTQIPQRYVEALKQQPKFKLTLA
NGQQINSEQLTLFTFVDPKSHSYKVRINLPENTDGLIPGSLIKAQFVSGSRNTIFIPESSLLTMNELSAVYIQQNDAWVL
NQVRTGLSNNGKVEILAGLSEGDVVAKDAYHALLLLKQSNKP

Sequences:

>Translated_362_residues
MNNSQKNALKNTALLAYLLISSLPFLSLSASVSAESRAAADNKHLESITVSFKPYPNWVQLDAVIEPTKSATVSAQTSGR
IIKLNYDINDVVATGAPLLEITSKEQGAQLAAAEAEYAKARAVNVEAQQQFKRYQALFPKGAISKGAMDEAEANAKSAQQ
AVSAAKARITQASESLNYTLVSAPFAGIVTQRHVEEGETVSPGQALYSGYSLSQMRAVTQIPQRYVEALKQQPKFKLTLA
NGQQINSEQLTLFTFVDPKSHSYKVRINLPENTDGLIPGSLIKAQFVSGSRNTIFIPESSLLTMNELSAVYIQQNDAWVL
NQVRTGLSNNGKVEILAGLSEGDVVAKDAYHALLLLKQSNKP
>Mature_362_residues
MNNSQKNALKNTALLAYLLISSLPFLSLSASVSAESRAAADNKHLESITVSFKPYPNWVQLDAVIEPTKSATVSAQTSGR
IIKLNYDINDVVATGAPLLEITSKEQGAQLAAAEAEYAKARAVNVEAQQQFKRYQALFPKGAISKGAMDEAEANAKSAQQ
AVSAAKARITQASESLNYTLVSAPFAGIVTQRHVEEGETVSPGQALYSGYSLSQMRAVTQIPQRYVEALKQQPKFKLTLA
NGQQINSEQLTLFTFVDPKSHSYKVRINLPENTDGLIPGSLIKAQFVSGSRNTIFIPESSLLTMNELSAVYIQQNDAWVL
NQVRTGLSNNGKVEILAGLSEGDVVAKDAYHALLLLKQSNKP

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=328, Percent_Identity=24.390243902439, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789929, Length=347, Percent_Identity=23.6311239193084, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786668, Length=278, Percent_Identity=29.136690647482, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789665, Length=367, Percent_Identity=25.3405994550409, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39144; Mature: 39144

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSQKNALKNTALLAYLLISSLPFLSLSASVSAESRAAADNKHLESITVSFKPYPNWVQ
CCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHCCCCCCEEEEEEEECCCCCEEE
LDAVIEPTKSATVSAQTSGRIIKLNYDINDVVATGAPLLEITSKEQGAQLAAAEAEYAKA
EEEEECCCCCCEEEECCCCEEEEEECCHHHHHHCCCCEEEECCCCCCCEEEHHHHHHHHH
RAVNVEAQQQFKRYQALFPKGAISKGAMDEAEANAKSAQQAVSAAKARITQASESLNYTL
HEECHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VSAPFAGIVTQRHVEEGETVSPGQALYSGYSLSQMRAVTQIPQRYVEALKQQPKFKLTLA
EECCHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
NGQQINSEQLTLFTFVDPKSHSYKVRINLPENTDGLIPGSLIKAQFVSGSRNTIFIPESS
CCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHEEEECCCCCEEEECCCC
LLTMNELSAVYIQQNDAWVLNQVRTGLSNNGKVEILAGLSEGDVVAKDAYHALLLLKQSN
EEECCCCEEEEEECCCCEEHHHHHHCCCCCCCEEEEEECCCCCEEHHHHHEEEEEEECCC
KP
CC
>Mature Secondary Structure
MNNSQKNALKNTALLAYLLISSLPFLSLSASVSAESRAAADNKHLESITVSFKPYPNWVQ
CCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHCCCCCCEEEEEEEECCCCCEEE
LDAVIEPTKSATVSAQTSGRIIKLNYDINDVVATGAPLLEITSKEQGAQLAAAEAEYAKA
EEEEECCCCCCEEEECCCCEEEEEECCHHHHHHCCCCEEEECCCCCCCEEEHHHHHHHHH
RAVNVEAQQQFKRYQALFPKGAISKGAMDEAEANAKSAQQAVSAAKARITQASESLNYTL
HEECHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VSAPFAGIVTQRHVEEGETVSPGQALYSGYSLSQMRAVTQIPQRYVEALKQQPKFKLTLA
EECCHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
NGQQINSEQLTLFTFVDPKSHSYKVRINLPENTDGLIPGSLIKAQFVSGSRNTIFIPESS
CCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHEEEECCCCCEEEECCCC
LLTMNELSAVYIQQNDAWVLNQVRTGLSNNGKVEILAGLSEGDVVAKDAYHALLLLKQSN
EEECCCCEEEEEECCCCEEHHHHHHCCCCCCCEEEEEECCCCCEEHHHHHEEEEEEECCC
KP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]