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The map label for this gene is puuB [H]

Identifier: 157962376

GI number: 157962376

Start: 3108354

End: 3109643

Strand: Reverse

Name: puuB [H]

Synonym: Spea_2555

Alternate gene names: 157962376

Gene position: 3109643-3108354 (Counterclockwise)

Preceding gene: 157962378

Following gene: 157962373

Centisome position: 60.09

GC content: 46.05

Gene sequence:

>1290_bases
ATGTCTGAGCCACGCTGCAACTCGTATTATAACGCTACGATCAATGAGGAAACCCAATATCCAGAGCTAGAGGGGGAGCT
TAGAGTCGATGTAGTAATAGTTGGTGGAGGCTTTACTGGGGTCGCGACGGCGTTAGAGCTCTCAGAGAAAGGCTATAAAG
TTGCCTTGCTCGAGGCGAATAAAATTGGTTGGGGCGCGACAGGTCGCAATGGTGGTCAGGTAACGGGAAGCCTTTCTGGC
GATGAGGCGATGACAAAACAGCTTAGAAGCCGGATCGGTAGTGATGCAGAGCAGTATGTTTGGAATATGCGCTGGCGTGG
GCATGAGATCATTAAAGAACGAGTCAAAAAGTATGATATTGCCTGTGACTTAAAGTTTGGTCATCTGCATACAGCCTACA
AGCCTAGCCACTTAAAAGAGCTTGAAAAAACCTATGCCGAAGCGTGCCTTAGAGGTATGGAAAGTGAATTGGCTATGCTG
AACAAACCGGAATTAGCCGATTATATTGATACGCCTTTATATCACGGCGGACTACTTAACAAACGCAATATGCATCTACA
CTCGGTTAACCTTTGTATTGGTGAGGCGAGGGCTGCAGAAAGCCTTGGGTCATTAATTTTTGAGCATTCTGCAGTTGTAG
ATATCGATGATGGCACATTAGCAACGGTGACGACAGCCAAAGGTAAAGTGGTGGCTAATCGGGTAGTGTTAGCTGGTAAT
GCATATCATAAACTTGCTCGTAAAAAATTACGCGGCATGTTGTTTCCTGCATCCTTGGGTAATTGTGCGACGGCTCAGCT
ATCACCAGAACTGGCGATGAGTTTAATTCCCAAAGATGTTGCCGTATACGACACTCGTTTCGTGCTGGATTATTATCGTT
TAACTGCCGATAACCGTTTGATGTTTGGCGGTGGTACTAATTATTCGGGTCGAGATTCCAAAGACGTTGCTGGAGAGTTA
CGACCCGCGATTGAGCGGACCTTTCCACAACTTAAAGGCGTGGATATTGAGTTTGGTTGGACGGGTATGGCGGGGATAGT
GCTTAATCGTATCCCTCAGTTAGGCAAAGTGTCACCCAATGTGTATTACTGCCAAGGTTATTCAGGACATGGTGTAGCGA
CATCGCATATTATGGGGGAAATTATGGCTAATGCTGTAGCCGGAGAGCTGCAAGAGTTTGATTTGTTTGCACAGATGTCC
CATTACCGGATCCCACTTAATGAATGGTTTGGTAATCAAGCTTTAGCGCTGGGGATGATGTATTACAAGGTGATGGAAAA
CTTCCGCTAA

Upstream 100 bases:

>100_bases
CAAGCCTTGACCTGCCTAGTTAGATGGATATACTGAACAAAAGGTGAGAAGTGTAAAAAATATAAAACAAGAAGTAACAG
ATTACTCAAAAGGAATGAAG

Downstream 100 bases:

>100_bases
TACAGGTTTGGGAAACTGAGCTTATAAAAAATCCGCTTCGATAGCGGATTTTTTTGGTCGTGCTTTTTGATAGAGTGAAT
GATTTAGATATCGACCTTTT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSEPRCNSYYNATINEETQYPELEGELRVDVVIVGGGFTGVATALELSEKGYKVALLEANKIGWGATGRNGGQVTGSLSG
DEAMTKQLRSRIGSDAEQYVWNMRWRGHEIIKERVKKYDIACDLKFGHLHTAYKPSHLKELEKTYAEACLRGMESELAML
NKPELADYIDTPLYHGGLLNKRNMHLHSVNLCIGEARAAESLGSLIFEHSAVVDIDDGTLATVTTAKGKVVANRVVLAGN
AYHKLARKKLRGMLFPASLGNCATAQLSPELAMSLIPKDVAVYDTRFVLDYYRLTADNRLMFGGGTNYSGRDSKDVAGEL
RPAIERTFPQLKGVDIEFGWTGMAGIVLNRIPQLGKVSPNVYYCQGYSGHGVATSHIMGEIMANAVAGELQEFDLFAQMS
HYRIPLNEWFGNQALALGMMYYKVMENFR

Sequences:

>Translated_429_residues
MSEPRCNSYYNATINEETQYPELEGELRVDVVIVGGGFTGVATALELSEKGYKVALLEANKIGWGATGRNGGQVTGSLSG
DEAMTKQLRSRIGSDAEQYVWNMRWRGHEIIKERVKKYDIACDLKFGHLHTAYKPSHLKELEKTYAEACLRGMESELAML
NKPELADYIDTPLYHGGLLNKRNMHLHSVNLCIGEARAAESLGSLIFEHSAVVDIDDGTLATVTTAKGKVVANRVVLAGN
AYHKLARKKLRGMLFPASLGNCATAQLSPELAMSLIPKDVAVYDTRFVLDYYRLTADNRLMFGGGTNYSGRDSKDVAGEL
RPAIERTFPQLKGVDIEFGWTGMAGIVLNRIPQLGKVSPNVYYCQGYSGHGVATSHIMGEIMANAVAGELQEFDLFAQMS
HYRIPLNEWFGNQALALGMMYYKVMENFR
>Mature_428_residues
SEPRCNSYYNATINEETQYPELEGELRVDVVIVGGGFTGVATALELSEKGYKVALLEANKIGWGATGRNGGQVTGSLSGD
EAMTKQLRSRIGSDAEQYVWNMRWRGHEIIKERVKKYDIACDLKFGHLHTAYKPSHLKELEKTYAEACLRGMESELAMLN
KPELADYIDTPLYHGGLLNKRNMHLHSVNLCIGEARAAESLGSLIFEHSAVVDIDDGTLATVTTAKGKVVANRVVLAGNA
YHKLARKKLRGMLFPASLGNCATAQLSPELAMSLIPKDVAVYDTRFVLDYYRLTADNRLMFGGGTNYSGRDSKDVAGELR
PAIERTFPQLKGVDIEFGWTGMAGIVLNRIPQLGKVSPNVYYCQGYSGHGVATSHIMGEIMANAVAGELQEFDLFAQMSH
YRIPLNEWFGNQALALGMMYYKVMENFR

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=428, Percent_Identity=40.4205607476636, Blast_Score=314, Evalue=6e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47429; Mature: 47298

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPRCNSYYNATINEETQYPELEGELRVDVVIVGGGFTGVATALELSEKGYKVALLEAN
CCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEC
KIGWGATGRNGGQVTGSLSGDEAMTKQLRSRIGSDAEQYVWNMRWRGHEIIKERVKKYDI
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHCCC
ACDLKFGHLHTAYKPSHLKELEKTYAEACLRGMESELAMLNKPELADYIDTPLYHGGLLN
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHCCCCCCCCCCC
KRNMHLHSVNLCIGEARAAESLGSLIFEHSAVVDIDDGTLATVTTAKGKVVANRVVLAGN
CCCCEEEEEEEEECHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCEEEEEEEEEECC
AYHKLARKKLRGMLFPASLGNCATAQLSPELAMSLIPKDVAVYDTRFVLDYYRLTADNRL
HHHHHHHHHHHCEECCCCCCCCEECCCCHHHHHHHCCCCHHHHHHHHEEEHHHCCCCCEE
MFGGGTNYSGRDSKDVAGELRPAIERTFPQLKGVDIEFGWTGMAGIVLNRIPQLGKVSPN
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCCCCCCC
VYYCQGYSGHGVATSHIMGEIMANAVAGELQEFDLFAQMSHYRIPLNEWFGNQALALGMM
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHH
YYKVMENFR
HHHHHHHCC
>Mature Secondary Structure 
SEPRCNSYYNATINEETQYPELEGELRVDVVIVGGGFTGVATALELSEKGYKVALLEAN
CCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEC
KIGWGATGRNGGQVTGSLSGDEAMTKQLRSRIGSDAEQYVWNMRWRGHEIIKERVKKYDI
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHCCC
ACDLKFGHLHTAYKPSHLKELEKTYAEACLRGMESELAMLNKPELADYIDTPLYHGGLLN
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHCCCCCCCCCCC
KRNMHLHSVNLCIGEARAAESLGSLIFEHSAVVDIDDGTLATVTTAKGKVVANRVVLAGN
CCCCEEEEEEEEECHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCEEEEEEEEEECC
AYHKLARKKLRGMLFPASLGNCATAQLSPELAMSLIPKDVAVYDTRFVLDYYRLTADNRL
HHHHHHHHHHHCEECCCCCCCCEECCCCHHHHHHHCCCCHHHHHHHHEEEHHHCCCCCEE
MFGGGTNYSGRDSKDVAGELRPAIERTFPQLKGVDIEFGWTGMAGIVLNRIPQLGKVSPN
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCCCCCCC
VYYCQGYSGHGVATSHIMGEIMANAVAGELQEFDLFAQMSHYRIPLNEWFGNQALALGMM
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHH
YYKVMENFR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]