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The map label for this gene is rluE [H]

Identifier: 157962357

GI number: 157962357

Start: 3088087

End: 3088821

Strand: Direct

Name: rluE [H]

Synonym: Spea_2536

Alternate gene names: 157962357

Gene position: 3088087-3088821 (Clockwise)

Preceding gene: 157962355

Following gene: 157962358

Centisome position: 59.68

GC content: 43.95

Gene sequence:

>735_bases
GTGACACAGCCATTTTCAAAAAACAGAACCAGCAACAAATCGCCCAGTAAAAGCAATAAGCCGGGCGGCTTCGGCAACGC
TGCCAGCAAACAAGCTGGAAACGCTAAAGTCCAAGCAAGAAGCGGACAGCCTCATAATAGAGTCAATAGCAAGACTAAGC
CGAAAGCAAAATCAGTTACCAACCCAATAATCGTGCTATTCAATAAACCTTTCGATGTACTATGTCAGTTCACCGATGAG
CAAGGGCGGCAAACCCTTAAAGACTTTATCCCGATACCCGGCGTTTATGCCGCGGGTCGTTTAGATAGAGACAGTGAGGG
CCTGCTTCTGTTGACCAACGACGGTAAATTACAATCACAAATTACCGAGCCTAAGAAGAAAACCTATAAAACCTATTGGG
CTCAAGTTGAAGGCGCACCTAACGAGGATGACCTTGAAAAACTACGTCAAGGTGTTGAATTAAAAGATGGTATGAGTTTA
CCAGCCAAAATCAGCATTATGAATAACCCAGACATTTGGCCAAGAACGCCGCCTATTCGTGAGCGTGCGAACATTCCTAC
TACCTGGTTAGAAATTAAGATCTGTGAAGGCAGAAACCGTCAAGTGAGACGCATGACGGCGCATATCGGTTTCCCTACAT
TAAGATTGATCCGCTATAAAATAGGTCAATGGAGTCTAGATGACCTAAAAAATGGTGAATATCGTCAACTTGAAATGAAT
AATAAGTCGGTGTGA

Upstream 100 bases:

>100_bases
GCCGACATCATAAAACATTCCGCGCAAGATTCAAATTCCACTAAAAGCCAATGCACAGTACAATAGGTATAAATCACTCA
AAGATTAAAAGTATTACAGC

Downstream 100 bases:

>100_bases
GTAACAAGCAATGACTGAGCGCTATAAACCCAACACCACTGTCGCCTGCATGATTGAGGCGCAAGGGAAGTATCTTTTAG
TCGAAGAACTCATTGAAGGT

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MTQPFSKNRTSNKSPSKSNKPGGFGNAASKQAGNAKVQARSGQPHNRVNSKTKPKAKSVTNPIIVLFNKPFDVLCQFTDE
QGRQTLKDFIPIPGVYAAGRLDRDSEGLLLLTNDGKLQSQITEPKKKTYKTYWAQVEGAPNEDDLEKLRQGVELKDGMSL
PAKISIMNNPDIWPRTPPIRERANIPTTWLEIKICEGRNRQVRRMTAHIGFPTLRLIRYKIGQWSLDDLKNGEYRQLEMN
NKSV

Sequences:

>Translated_244_residues
MTQPFSKNRTSNKSPSKSNKPGGFGNAASKQAGNAKVQARSGQPHNRVNSKTKPKAKSVTNPIIVLFNKPFDVLCQFTDE
QGRQTLKDFIPIPGVYAAGRLDRDSEGLLLLTNDGKLQSQITEPKKKTYKTYWAQVEGAPNEDDLEKLRQGVELKDGMSL
PAKISIMNNPDIWPRTPPIRERANIPTTWLEIKICEGRNRQVRRMTAHIGFPTLRLIRYKIGQWSLDDLKNGEYRQLEMN
NKSV
>Mature_243_residues
TQPFSKNRTSNKSPSKSNKPGGFGNAASKQAGNAKVQARSGQPHNRVNSKTKPKAKSVTNPIIVLFNKPFDVLCQFTDEQ
GRQTLKDFIPIPGVYAAGRLDRDSEGLLLLTNDGKLQSQITEPKKKTYKTYWAQVEGAPNEDDLEKLRQGVELKDGMSLP
AKISIMNNPDIWPRTPPIRERANIPTTWLEIKICEGRNRQVRRMTAHIGFPTLRLIRYKIGQWSLDDLKNGEYRQLEMNN
KSV

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=193, Percent_Identity=61.6580310880829, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1788510, Length=200, Percent_Identity=34, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1787524, Length=180, Percent_Identity=31.1111111111111, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1790453, Length=176, Percent_Identity=28.4090909090909, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 27559; Mature: 27428

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPFSKNRTSNKSPSKSNKPGGFGNAASKQAGNAKVQARSGQPHNRVNSKTKPKAKSVT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHCCCCCCCCHHHCC
NPIIVLFNKPFDVLCQFTDEQGRQTLKDFIPIPGVYAAGRLDRDSEGLLLLTNDGKLQSQ
CCEEEEECCCHHHEEECCCHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEECCCCHHHH
ITEPKKKTYKTYWAQVEGAPNEDDLEKLRQGVELKDGMSLPAKISIMNNPDIWPRTPPIR
HCCHHHHHHHHHHEEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCH
ERANIPTTWLEIKICEGRNRQVRRMTAHIGFPTLRLIRYKIGQWSLDDLKNGEYRQLEMN
HHCCCCEEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHCCCCCHHHCCCCCEEEEECC
NKSV
CCCC
>Mature Secondary Structure 
TQPFSKNRTSNKSPSKSNKPGGFGNAASKQAGNAKVQARSGQPHNRVNSKTKPKAKSVT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHCCCCCCCCHHHCC
NPIIVLFNKPFDVLCQFTDEQGRQTLKDFIPIPGVYAAGRLDRDSEGLLLLTNDGKLQSQ
CCEEEEECCCHHHEEECCCHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEECCCCHHHH
ITEPKKKTYKTYWAQVEGAPNEDDLEKLRQGVELKDGMSLPAKISIMNNPDIWPRTPPIR
HCCHHHHHHHHHHEEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCH
ERANIPTTWLEIKICEGRNRQVRRMTAHIGFPTLRLIRYKIGQWSLDDLKNGEYRQLEMN
HHCCCCEEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHCCCCCHHHCCCCCEEEEECC
NKSV
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]