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The map label for this gene is cspD [H]

Identifier: 157962355

GI number: 157962355

Start: 3085187

End: 3085393

Strand: Direct

Name: cspD [H]

Synonym: Spea_2534

Alternate gene names: 157962355

Gene position: 3085187-3085393 (Clockwise)

Preceding gene: 157962352

Following gene: 157962357

Centisome position: 59.62

GC content: 45.89

Gene sequence:

>207_bases
ATGGCAAGCGGAACTGTTAAATGGTTCAACAACGCCAAAGGATTCGGGTTTATATGCCCTGATGCTGGCGGTGAAGACGT
TTTTGCACACTATTCTACCATTGAAATGGAAGGCTATCGAACTCTAAAAGCAGGCCAACCAGTCAGTTTTGAAGTAGAAG
CAGGTCCAAAAGGGATGCACGCGTCAGCAATATCGCCAACAAACTGA

Upstream 100 bases:

>100_bases
CCACTTATTCAGCTTTCATTCTGTTCATTAAGCGGGGAGTTTCCTCGCTGATAAGTTTTACTATCTTACTGGGAAGTAGA
CAACAGAAGGAAGTGGAAGT

Downstream 100 bases:

>100_bases
TACCAATCTAGCTAAGTAAGTGTCTAGAAATAATTAAACAATACTTGTTTAGCTTGGTATAGGTTGCGATAAAAGATAAA
AAAAGCAGAGTAGTTAATCT

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: CSP-D [H]

Number of amino acids: Translated: 68; Mature: 67

Protein sequence:

>68_residues
MASGTVKWFNNAKGFGFICPDAGGEDVFAHYSTIEMEGYRTLKAGQPVSFEVEAGPKGMHASAISPTN

Sequences:

>Translated_68_residues
MASGTVKWFNNAKGFGFICPDAGGEDVFAHYSTIEMEGYRTLKAGQPVSFEVEAGPKGMHASAISPTN
>Mature_67_residues
ASGTVKWFNNAKGFGFICPDAGGEDVFAHYSTIEMEGYRTLKAGQPVSFEVEAGPKGMHASAISPTN

Specific function: Inhibits DNA replication at both initiation and elongation steps, most probably by binding to the opened, single- stranded regions at replication forks. Plays a regulatory role in chromosomal replication in nutrient-depleted cells [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=76.1194029850746, Blast_Score=114, Evalue=9e-28,
Organism=Escherichia coli, GI1789979, Length=63, Percent_Identity=53.968253968254, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1787839, Length=66, Percent_Identity=51.5151515151515, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=52.4590163934426, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1788126, Length=62, Percent_Identity=51.6129032258064, Blast_Score=71, Evalue=1e-14,
Organism=Escherichia coli, GI2367114, Length=62, Percent_Identity=48.3870967741936, Blast_Score=70, Evalue=2e-14,
Organism=Escherichia coli, GI1787834, Length=65, Percent_Identity=49.2307692307692, Blast_Score=68, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI71983225, Length=64, Percent_Identity=43.75, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71983217, Length=64, Percent_Identity=43.75, Blast_Score=63, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24658520, Length=62, Percent_Identity=45.1612903225806, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7212; Mature: 7081

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGTVKWFNNAKGFGFICPDAGGEDVFAHYSTIEMEGYRTLKAGQPVSFEVEAGPKGMH
CCCCEEEECCCCCCCEEECCCCCCCCEEEEEEEEEECCCEEECCCCCEEEEECCCCCCCC
ASAISPTN
CCCCCCCC
>Mature Secondary Structure 
ASGTVKWFNNAKGFGFICPDAGGEDVFAHYSTIEMEGYRTLKAGQPVSFEVEAGPKGMH
CCCEEEECCCCCCCEEECCCCCCCCEEEEEEEEEECCCEEECCCCCEEEEECCCCCCCC
ASAISPTN
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]