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The map label for this gene is nfnB [C]

Identifier: 157962331

GI number: 157962331

Start: 3058779

End: 3059435

Strand: Direct

Name: nfnB [C]

Synonym: Spea_2510

Alternate gene names: 157962331

Gene position: 3058779-3059435 (Clockwise)

Preceding gene: 157962329

Following gene: 157962333

Centisome position: 59.11

GC content: 43.38

Gene sequence:

>657_bases
ATGACTCACCCTATTATTGCTGATCTTCAAAGCCGTTATACAACAAAGCGCTATGATGCGTCTAAGCGTATTCCTCAAGC
TGATCTTGATGTGATCTACCAAGCAATGAACCTTTCGGCCTCTTCTATCAATTCACAACCTTGGAAGTTCATTGTAATTG
AGAGCGACGAAGCTAAGCAGCGCATGCATGATACCTTTGCCAACAAGTTTCAATTTAATCAGCCGCATATTAAATCTGCA
TCACATATCGTACTCTTCGCTCACAACCCTAAATACACGCGTGAAGATTACGCAAAAGTTATCGATAAAGGTATTGCCGA
TGGCCGTATGGGAGTTGAAGACAGAGAGCAAGCATTTGGCGCTTTTGCTTTCGTCGAGTTAAATACCGATGAATCGGGTA
ACAACGCTGCTTGGACTAAGTCTCAGGCCTATTTAGCCTTAGGCAATACACTGCATACCCTTGCTCGTTTAGGTATCGAC
TCCACGACAATGGAAGGAGTTGATAACGAGTTAATTGGTGAGATCTTTAAAGAAGAGCTTGGTGGTTACCAATGTGATGT
CGCTTTAGCAATGGGCTACCATGATTCTGAGGAAGACTATAATGCACCTCTACCTAAGTCACGCCTTGCCATGAAGGATG
TTGTTCGAGTCCTTTAG

Upstream 100 bases:

>100_bases
TGATAATCATTCAACTAAAACACTGTATTTCGAAGATGCTGTTCGATGTAGGATAGCCACAACTTAATTTTGCTTCACCT
TTGTTCACTAGGATACATAT

Downstream 100 bases:

>100_bases
GTCTGTTGAGTAGATAACAACGGATAACTAAAGCACAATTAAAGTATAACTAGGCTGATATGTAGTAGCTTAGCAGATAA
AGGTTCGAGGCTTTTTCCTC

Product: nitroreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: FRASE I [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTHPIIADLQSRYTTKRYDASKRIPQADLDVIYQAMNLSASSINSQPWKFIVIESDEAKQRMHDTFANKFQFNQPHIKSA
SHIVLFAHNPKYTREDYAKVIDKGIADGRMGVEDREQAFGAFAFVELNTDESGNNAAWTKSQAYLALGNTLHTLARLGID
STTMEGVDNELIGEIFKEELGGYQCDVALAMGYHDSEEDYNAPLPKSRLAMKDVVRVL

Sequences:

>Translated_218_residues
MTHPIIADLQSRYTTKRYDASKRIPQADLDVIYQAMNLSASSINSQPWKFIVIESDEAKQRMHDTFANKFQFNQPHIKSA
SHIVLFAHNPKYTREDYAKVIDKGIADGRMGVEDREQAFGAFAFVELNTDESGNNAAWTKSQAYLALGNTLHTLARLGID
STTMEGVDNELIGEIFKEELGGYQCDVALAMGYHDSEEDYNAPLPKSRLAMKDVVRVL
>Mature_217_residues
THPIIADLQSRYTTKRYDASKRIPQADLDVIYQAMNLSASSINSQPWKFIVIESDEAKQRMHDTFANKFQFNQPHIKSAS
HIVLFAHNPKYTREDYAKVIDKGIADGRMGVEDREQAFGAFAFVELNTDESGNNAAWTKSQAYLALGNTLHTLARLGIDS
TTMEGVDNELIGEIFKEELGGYQCDVALAMGYHDSEEDYNAPLPKSRLAMKDVVRVL

Specific function: Involved in bioluminescence. It is a good supplier of reduced flavin mononucleotide (FMNH2) to the bioluminescence reaction. Major FMN reductase. It is capable of using both NADH and NADPH as electron donors. As electron acceptor, FMN is the most effectiv

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=207, Percent_Identity=35.2657004830918, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 24510; Mature: 24379

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPIIADLQSRYTTKRYDASKRIPQADLDVIYQAMNLSASSINSQPWKFIVIESDEAKQ
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCCCCEEEEEEECCHHHH
RMHDTFANKFQFNQPHIKSASHIVLFAHNPKYTREDYAKVIDKGIADGRMGVEDREQAFG
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCC
AFAFVELNTDESGNNAAWTKSQAYLALGNTLHTLARLGIDSTTMEGVDNELIGEIFKEEL
EEEEEEECCCCCCCCCCEECCCEEEEHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHH
GGYQCDVALAMGYHDSEEDYNAPLPKSRLAMKDVVRVL
CCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure 
THPIIADLQSRYTTKRYDASKRIPQADLDVIYQAMNLSASSINSQPWKFIVIESDEAKQ
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCCCCEEEEEEECCHHHH
RMHDTFANKFQFNQPHIKSASHIVLFAHNPKYTREDYAKVIDKGIADGRMGVEDREQAFG
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCC
AFAFVELNTDESGNNAAWTKSQAYLALGNTLHTLARLGIDSTTMEGVDNELIGEIFKEEL
EEEEEEECCCCCCCCCCEECCCEEEEHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHH
GGYQCDVALAMGYHDSEEDYNAPLPKSRLAMKDVVRVL
CCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8206830; 8033996; 9654450 [H]