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The map label for this gene is gph [C]

Identifier: 157962323

GI number: 157962323

Start: 3047445

End: 3048047

Strand: Reverse

Name: gph [C]

Synonym: Spea_2502

Alternate gene names: 157962323

Gene position: 3048047-3047445 (Counterclockwise)

Preceding gene: 157962324

Following gene: 157962320

Centisome position: 58.9

GC content: 42.62

Gene sequence:

>603_bases
ATGGATTCCATCAGTAAAATTGTTACTTGTATGCAGCAGATGGCTCACTCGCTATCTCTTGTTTGCCCATCAGAGTTGGC
GGTACGGGATGTGATTGGACTATCGATGGAAGAGGCGTTAAAAACCTTATATCCAGCTTTGCCTACAATTGATTTCGACC
CCATGATTAATTCGTATAAAGAGCATTACTTGACGCTTAATACCACACCAAGCCCATTGTTTGACGGTAGTGAAATGCTA
CTGAATGAGCTGAGTACACGTGATTATCGTTTAGCTGTTGCTACGGGTAAAGGGCGCAACGGTTTGAATCGCGTGCTAAG
TGAAACTGGATTAGCAAGGCATTTTGAATCGAGTCGATGCGCCGATGAGTCAAAGAGTAAACCAAATCCAGATATGCTCT
ATGACTTATTAGAAGAGCTTAAGGTTAAGCCTGAACGTGCGCTTATGGTTGGAGACTCTCTTCATGATCTCAATATGGCA
AATAATGCAGGGGTTGATTCAGTTGGCGTGAGTTATGGGGCTCATAGTGAGTCAAAGCTATTGTTAGCTAAGCCAAAGGC
TATTATCCATAGACCATTAGAGTTACTTAACGTATTAGCCTAG

Upstream 100 bases:

>100_bases
TTAAAGCACCATTAGACACAATACTCGAATCAACCTTAAAGAAGTTAAAGAAAGTATGAAGCCTTACGAATTAGTCATCT
TTGATTGGGATGGAACCTTA

Downstream 100 bases:

>100_bases
AACCGCTGTTTTATCGCTTAAACACCATTAATTGTTAGCTGGCTATGTGATGTGCGTTGGAGCGAGTAACAGAATCTTTA
CCACCTTTAGCGCTTAGTAT

Product: HAD family hydrolase

Products: Glycolate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MDSISKIVTCMQQMAHSLSLVCPSELAVRDVIGLSMEEALKTLYPALPTIDFDPMINSYKEHYLTLNTTPSPLFDGSEML
LNELSTRDYRLAVATGKGRNGLNRVLSETGLARHFESSRCADESKSKPNPDMLYDLLEELKVKPERALMVGDSLHDLNMA
NNAGVDSVGVSYGAHSESKLLLAKPKAIIHRPLELLNVLA

Sequences:

>Translated_200_residues
MDSISKIVTCMQQMAHSLSLVCPSELAVRDVIGLSMEEALKTLYPALPTIDFDPMINSYKEHYLTLNTTPSPLFDGSEML
LNELSTRDYRLAVATGKGRNGLNRVLSETGLARHFESSRCADESKSKPNPDMLYDLLEELKVKPERALMVGDSLHDLNMA
NNAGVDSVGVSYGAHSESKLLLAKPKAIIHRPLELLNVLA
>Mature_200_residues
MDSISKIVTCMQQMAHSLSLVCPSELAVRDVIGLSMEEALKTLYPALPTIDFDPMINSYKEHYLTLNTTPSPLFDGSEML
LNELSTRDYRLAVATGKGRNGLNRVLSETGLARHFESSRCADESKSKPNPDMLYDLLEELKVKPERALMVGDSLHDLNMA
NNAGVDSVGVSYGAHSESKLLLAKPKAIIHRPLELLNVLA

Specific function: Unknown

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.18 [C]

Molecular weight: Translated: 22000; Mature: 22000

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSISKIVTCMQQMAHSLSLVCPSELAVRDVIGLSMEEALKTLYPALPTIDFDPMINSYK
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHH
EHYLTLNTTPSPLFDGSEMLLNELSTRDYRLAVATGKGRNGLNRVLSETGLARHFESSRC
HHEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
ADESKSKPNPDMLYDLLEELKVKPERALMVGDSLHDLNMANNAGVDSVGVSYGAHSESKL
CHHCCCCCCHHHHHHHHHHHCCCCHHHEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCE
LLAKPKAIIHRPLELLNVLA
EEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDSISKIVTCMQQMAHSLSLVCPSELAVRDVIGLSMEEALKTLYPALPTIDFDPMINSYK
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHH
EHYLTLNTTPSPLFDGSEMLLNELSTRDYRLAVATGKGRNGLNRVLSETGLARHFESSRC
HHEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
ADESKSKPNPDMLYDLLEELKVKPERALMVGDSLHDLNMANNAGVDSVGVSYGAHSESKL
CHHCCCCCCHHHHHHHHHHHCCCCHHHEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCE
LLAKPKAIIHRPLELLNVLA
EEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.57 {phosphoglycolate}} [C]

Substrates: D-Glycerate 2-phosphatelyc; H2O [C]

Specific reaction: D-Glycerate 2-phosphatelyc + H2O --> Glycolate + Phosphate [C]

General reaction: Additional information:

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12368430; 12218036 [H]