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The map label for this gene is nth [H]

Identifier: 157962180

GI number: 157962180

Start: 2875294

End: 2875935

Strand: Direct

Name: nth [H]

Synonym: Spea_2359

Alternate gene names: 157962180

Gene position: 2875294-2875935 (Clockwise)

Preceding gene: 157962179

Following gene: 157962181

Centisome position: 55.57

GC content: 46.26

Gene sequence:

>642_bases
ATGAATACAGAGAAGCGCCGCCAGATCCTTGAAAGACTTAGAGAGAATAATCCTCATCCTGAAACTGAGCTTAATTTCTC
AAGCCCTTTTGAGCTGCTGGTTGCCGTTACATTATCAGCACAGGCTACGGACGTTAGTGTTAATAAAGCCACCGATAAAC
TGTTCCCGGTAGCCAATACGGCTCAGGCTATTGCAGCCCTTGGCGTTGAAGGTCTTAAGCAATACATTAAAACCATCGGC
CTGTACAACAATAAAGCCATTAACGTGGTAAAAGCTTGTGAGATCTTAGTCGAAAAATACGATGGTGAGGTTCCAGAGGA
TCGTGAGGCACTCGAAGCCCTTCCCGGTGTTGGCAGAAAGACTGCCAATGTGGTACTCAACACGGCTTTTGGCTGGCCAA
CCATCGCGGTCGACACTCACATAGACAGGGTATCAAATCGCACTAAGTTCGCAATTGGCAAGAATGTCGTCGAAGTGGAA
AAAAAGCTGCTAAAAGTCGTTCCAGCCGAGTTTAAGGTCGATGTACACCACTGGCTTATATTACACGGTCGCTACACCTG
TATTGCCCGTAAACCTCGCTGTGGTAGCTGCATTATAGAAGATCTGTGTGAGTACAAAGATAAGGTTTATCCTGAAGACT
AG

Upstream 100 bases:

>100_bases
AACAGCCTAAAGTCGAGCAAGAAGCGCCTACTCGAGCGCGTATTACTAAGGTCAATTAAGCCGCAATAAATAATAACAAT
ACAGGATACGGATATAGATA

Downstream 100 bases:

>100_bases
CCCTATTGAGGCTCGACGAGTCGATTAAGTCGTGGTCATGGATTTACAGACGGACAGCTACAAAAAAAGCCGCTAAAAGC
GGCTTTTTTTGTAGCTGTAT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MNTEKRRQILERLRENNPHPETELNFSSPFELLVAVTLSAQATDVSVNKATDKLFPVANTAQAIAALGVEGLKQYIKTIG
LYNNKAINVVKACEILVEKYDGEVPEDREALEALPGVGRKTANVVLNTAFGWPTIAVDTHIDRVSNRTKFAIGKNVVEVE
KKLLKVVPAEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEYKDKVYPED

Sequences:

>Translated_213_residues
MNTEKRRQILERLRENNPHPETELNFSSPFELLVAVTLSAQATDVSVNKATDKLFPVANTAQAIAALGVEGLKQYIKTIG
LYNNKAINVVKACEILVEKYDGEVPEDREALEALPGVGRKTANVVLNTAFGWPTIAVDTHIDRVSNRTKFAIGKNVVEVE
KKLLKVVPAEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEYKDKVYPED
>Mature_213_residues
MNTEKRRQILERLRENNPHPETELNFSSPFELLVAVTLSAQATDVSVNKATDKLFPVANTAQAIAALGVEGLKQYIKTIG
LYNNKAINVVKACEILVEKYDGEVPEDREALEALPGVGRKTANVVLNTAFGWPTIAVDTHIDRVSNRTKFAIGKNVVEVE
KKLLKVVPAEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEYKDKVYPED

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=29.9435028248588, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1787920, Length=209, Percent_Identity=82.2966507177033, Blast_Score=357, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=32.5714285714286, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6324530, Length=192, Percent_Identity=26.0416666666667, Blast_Score=75, Evalue=6e-15,
Organism=Drosophila melanogaster, GI45550361, Length=178, Percent_Identity=33.1460674157303, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23841; Mature: 23841

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTEKRRQILERLRENNPHPETELNFSSPFELLVAVTLSAQATDVSVNKATDKLFPVANT
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHH
AQAIAALGVEGLKQYIKTIGLYNNKAINVVKACEILVEKYDGEVPEDREALEALPGVGRK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGWPTIAVDTHIDRVSNRTKFAIGKNVVEVEKKLLKVVPAEFKVDVHHWLI
HHHEEEECCCCCCEEEEHHHHHHHCCCCCHHHCCHHHHHHHHHHHHCCHHHEEEHHHEEE
LHGRYTCIARKPRCGSCIIEDLCEYKDKVYPED
EECCEEEEECCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNTEKRRQILERLRENNPHPETELNFSSPFELLVAVTLSAQATDVSVNKATDKLFPVANT
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHH
AQAIAALGVEGLKQYIKTIGLYNNKAINVVKACEILVEKYDGEVPEDREALEALPGVGRK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGWPTIAVDTHIDRVSNRTKFAIGKNVVEVEKKLLKVVPAEFKVDVHHWLI
HHHEEEECCCCCCEEEEHHHHHHHCCCCCHHHCCHHHHHHHHHHHHCCHHHEEEHHHEEE
LHGRYTCIARKPRCGSCIIEDLCEYKDKVYPED
EECCEEEEECCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]