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The map label for this gene is rnfE

Identifier: 157962179

GI number: 157962179

Start: 2874557

End: 2875252

Strand: Direct

Name: rnfE

Synonym: Spea_2358

Alternate gene names: 157962179

Gene position: 2874557-2875252 (Clockwise)

Preceding gene: 157962178

Following gene: 157962180

Centisome position: 55.55

GC content: 45.83

Gene sequence:

>696_bases
ATGAGTGATTATAAAGAGCTAACCAAACAAGGGCTCTGGAAAAACAATCCTGGTCTAGTGCAGCTTTTGGGCCTGTGCCC
TCTTCTGGCCGTTACCGCAACCTTGACCAACGCCCTAGGTTTAGGTCTTGCGACCATGCTGGTATTAATCGGCTCAAACA
TCTTGGTCTCTCTGGTGCGTGACTTTGTACCAAAAGAGATCCGCATTCCAGTGTTTGTGATGATCATTGCAGCGCTAGTA
ACAACCGTGCAGTTATTAATAAATGCATACGCTTACGGCCTGTACTTATCACTTGGGATCTTCTTGCCACTTATCGTCAC
TAACTGCGTCATTATTGGCCGCGCCGAGGCTTTTGCATCACGTAATAGTGTTGCAAAATCAGCCTTTGACGGGCTCATGA
TGGGATTAGGTTTTACCTTAGTCTTATCTGTTTTAGGTGCAGCTCGTGAAATATTAGGCCAAGGCACACTATTTTACGGC
GCCGACCAACTGTTAGGTGAATGGGCTAAGTCATTAACCATTCATATCTGGCAGGTCAACACTAGCTTTCTGTTAGCCAT
GCTACCACCTGGTGCCTTTATCGGTATGGGCTTACTCATCGCACTTAAAAACGCCATAGATCAATATCTTGCTACGAAAC
AGCCTAAAGTCGAGCAAGAAGCGCCTACTCGAGCGCGTATTACTAAGGTCAATTAA

Upstream 100 bases:

>100_bases
CACCACGTGCATATGTAAAAGCCGTTAAGAATGCCGTTAGCTATTTTAATGCCAACAAGGCACAGCTGTTATCGACACCT
GCCAATTGCGAGGTTGGAAA

Downstream 100 bases:

>100_bases
GCCGCAATAAATAATAACAATACAGGATACGGATATAGATAATGAATACAGAGAAGCGCCGCCAGATCCTTGAAAGACTT
AGAGAGAATAATCCTCATCC

Product: electron transport complex RsxE subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSDYKELTKQGLWKNNPGLVQLLGLCPLLAVTATLTNALGLGLATMLVLIGSNILVSLVRDFVPKEIRIPVFVMIIAALV
TTVQLLINAYAYGLYLSLGIFLPLIVTNCVIIGRAEAFASRNSVAKSAFDGLMMGLGFTLVLSVLGAAREILGQGTLFYG
ADQLLGEWAKSLTIHIWQVNTSFLLAMLPPGAFIGMGLLIALKNAIDQYLATKQPKVEQEAPTRARITKVN

Sequences:

>Translated_231_residues
MSDYKELTKQGLWKNNPGLVQLLGLCPLLAVTATLTNALGLGLATMLVLIGSNILVSLVRDFVPKEIRIPVFVMIIAALV
TTVQLLINAYAYGLYLSLGIFLPLIVTNCVIIGRAEAFASRNSVAKSAFDGLMMGLGFTLVLSVLGAAREILGQGTLFYG
ADQLLGEWAKSLTIHIWQVNTSFLLAMLPPGAFIGMGLLIALKNAIDQYLATKQPKVEQEAPTRARITKVN
>Mature_230_residues
SDYKELTKQGLWKNNPGLVQLLGLCPLLAVTATLTNALGLGLATMLVLIGSNILVSLVRDFVPKEIRIPVFVMIIAALVT
TVQLLINAYAYGLYLSLGIFLPLIVTNCVIIGRAEAFASRNSVAKSAFDGLMMGLGFTLVLSVLGAAREILGQGTLFYGA
DQLLGEWAKSLTIHIWQVNTSFLLAMLPPGAFIGMGLLIALKNAIDQYLATKQPKVEQEAPTRARITKVN

Specific function: May be part of a membrane complex involved in electron transport

COG id: COG4660

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family

Homologues:

Organism=Escherichia coli, GI1787919, Length=210, Percent_Identity=62.8571428571429, Blast_Score=250, Evalue=6e-68,
Organism=Escherichia coli, GI1787914, Length=156, Percent_Identity=35.2564102564103, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNFE_SHEPA (A8H541)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001502213.1
- STRING:   A8H541
- GeneID:   5662751
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_2358
- NMPDR:   fig|398579.3.peg.2026
- eggNOG:   COG4660
- HOGENOM:   HBG624915
- OMA:   VTNCVII
- ProtClustDB:   PRK12405
- BioCyc:   SPEA398579:SPEA_2358-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00478
- InterPro:   IPR003667
- InterPro:   IPR010968
- PIRSF:   PIRSF006102
- TIGRFAMs:   TIGR01948

Pfam domain/function: PF02508 Rnf-Nqr

EC number: NA

Molecular weight: Translated: 24860; Mature: 24729

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b99b668)-; HASH(0x1bb8c9a0)-; HASH(0x1be709a0)-; HASH(0x1bac0718)-; HASH(0x1bd7a1a0)-; HASH(0x1b958a24)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYKELTKQGLWKNNPGLVQLLGLCPLLAVTATLTNALGLGLATMLVLIGSNILVSLVR
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
DFVPKEIRIPVFVMIIAALVTTVQLLINAYAYGLYLSLGIFLPLIVTNCVIIGRAEAFAS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
RNSVAKSAFDGLMMGLGFTLVLSVLGAAREILGQGTLFYGADQLLGEWAKSLTIHIWQVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHEEEEEEEC
TSFLLAMLPPGAFIGMGLLIALKNAIDQYLATKQPKVEQEAPTRARITKVN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECH
>Mature Secondary Structure 
SDYKELTKQGLWKNNPGLVQLLGLCPLLAVTATLTNALGLGLATMLVLIGSNILVSLVR
CHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
DFVPKEIRIPVFVMIIAALVTTVQLLINAYAYGLYLSLGIFLPLIVTNCVIIGRAEAFAS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
RNSVAKSAFDGLMMGLGFTLVLSVLGAAREILGQGTLFYGADQLLGEWAKSLTIHIWQVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHEEEEEEEC
TSFLLAMLPPGAFIGMGLLIALKNAIDQYLATKQPKVEQEAPTRARITKVN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA