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The map label for this gene is pykA [H]

Identifier: 157962158

GI number: 157962158

Start: 2846735

End: 2848171

Strand: Direct

Name: pykA [H]

Synonym: Spea_2337

Alternate gene names: 157962158

Gene position: 2846735-2848171 (Clockwise)

Preceding gene: 157962157

Following gene: 157962161

Centisome position: 55.01

GC content: 47.46

Gene sequence:

>1437_bases
ATGTTCCGCAGAACCAAAATTGTAACAACTTTGGGTCCAGCCACAGACCGTGATGACAACTTACGCCGCATTATTGCTGC
CGGTGCTAACGTTGTTCGCTTAAACTTCTCTCACGGTGCACCAGAAGATCATAAGAAACGCGCAGACGATACACGAGCGA
TTGCAAAAGAGCTCGGCGTTCATGTTGCCGTTTTAGGCGATCTACAGGGCCCTAAAATCCGAATCTCAACTTTCAAAGAC
AACAGAAAGGTTATGTTAGTGCTTGGTCAAGCCTATACCCTTGATGCCGATCTAGCGAAAGGTGAAGGTGATGAAAACCA
AGTCGGTATCGATTATAAAGAGTTACCACAGGACGTTAGTCTTGGTGATATCTTAATGCTCGATGACGGCCGCGTTCAAC
TACGCGTTGAAAGCGTCGAAGGTAACAAGGTTCATACCATAGTGACGGTTGCCGGTCCTTTATCAAACAATAAAGGTATC
AACAAGCAAGGCGGTGGCCTTTCTGCGGCGGCTCTAACAGAAAAAGACAAGCAAGACATCATCACCGCAGCTGAGATTAA
AGTTGATTATCTCGCCGTCTCTTTCCCAAGAAGTGGTGCAGATCTTAACTACGCTCGTGATTTAGCACGTCAAGCCGGTA
GTAATGCGTTAATCGTCTCTAAAGTTGAGCGCGCAGAAGCCGTTGCAACTGACGAAGCGATGGACGATGTCATCATGGCG
TCTGATGCCGTTATGGTTGCTCGTGGTGATCTCGGTGTTGAAATTGGTGATCCTGCTCTTGTTGCAGTTCAAAAGAGACT
GATTAGCCGTTCACGTCAATTGAATAAGCCTGTTATTACAGCAACTCAAATGATGGAATCGATGATCTCTAGCCCAATGC
CAACTCGTGCAGAAGTGATGGACGTAGCAAACGCGGTTCTAGATGGTACCGATGCGGTAATGCTAAGCGCCGAAACTGCT
GCCGGTGACTTCCCAGAAGAAACCGTTAAAGCCATGGCTGAAGTCTGTTTAGGTGCGGAGTCTCATCCTAGTGTTCAAGT
TTCTAAGCACAGATTGGATCAGCAGTTCACCACCATCGAAGAGACGATTGCATTATCGACTATGTATGCAGCAAACCACC
TAGAAGGTATCAAGGCCATTATCGCCCTTACCGAATCTGGTGCAACGCCGCAGATGATGTCTCGCATCAGCTCTGCTCTG
CCAATTTTTGCCTTGTCACGTCATGAGTCGACTTTGGCTAAGATGGCGCTTTACCGCGGTGTTCAGCCTGTGTATTTTGA
TTCGACTGCACATGCTCCAGAAGCGGTAGCTCAAAAAGCACTAGAGACGCTTTCTCAAGCTGGTTACCTTGTAAAAGGCG
ACATGGCGATGATGACTAAGGGTGATGCAATGGAAACCGTTGGTGGCACTAACACCAGCAAGGTTGTTGTTGTTTAA

Upstream 100 bases:

>100_bases
AAAACTTGAGTTAGATCATTTTGTTCGTAAATTTACTACAAGTATGGGTGTTGTCTGTTAATATAGGGTCAGAATTATTT
AAAACTTAACGGAGTATCTC

Downstream 100 bases:

>100_bases
ACCATACTCCCCCTTAAATCATTAGGGATTTAGTCAATAAAAAAGGTACGCATTGCGTACCTTTTTTTATACTCAATAAA
CGGGTTAAAGTTTATCGAAC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGAPEDHKKRADDTRAIAKELGVHVAVLGDLQGPKIRISTFKD
NRKVMLVLGQAYTLDADLAKGEGDENQVGIDYKELPQDVSLGDILMLDDGRVQLRVESVEGNKVHTIVTVAGPLSNNKGI
NKQGGGLSAAALTEKDKQDIITAAEIKVDYLAVSFPRSGADLNYARDLARQAGSNALIVSKVERAEAVATDEAMDDVIMA
SDAVMVARGDLGVEIGDPALVAVQKRLISRSRQLNKPVITATQMMESMISSPMPTRAEVMDVANAVLDGTDAVMLSAETA
AGDFPEETVKAMAEVCLGAESHPSVQVSKHRLDQQFTTIEETIALSTMYAANHLEGIKAIIALTESGATPQMMSRISSAL
PIFALSRHESTLAKMALYRGVQPVYFDSTAHAPEAVAQKALETLSQAGYLVKGDMAMMTKGDAMETVGGTNTSKVVVV

Sequences:

>Translated_478_residues
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGAPEDHKKRADDTRAIAKELGVHVAVLGDLQGPKIRISTFKD
NRKVMLVLGQAYTLDADLAKGEGDENQVGIDYKELPQDVSLGDILMLDDGRVQLRVESVEGNKVHTIVTVAGPLSNNKGI
NKQGGGLSAAALTEKDKQDIITAAEIKVDYLAVSFPRSGADLNYARDLARQAGSNALIVSKVERAEAVATDEAMDDVIMA
SDAVMVARGDLGVEIGDPALVAVQKRLISRSRQLNKPVITATQMMESMISSPMPTRAEVMDVANAVLDGTDAVMLSAETA
AGDFPEETVKAMAEVCLGAESHPSVQVSKHRLDQQFTTIEETIALSTMYAANHLEGIKAIIALTESGATPQMMSRISSAL
PIFALSRHESTLAKMALYRGVQPVYFDSTAHAPEAVAQKALETLSQAGYLVKGDMAMMTKGDAMETVGGTNTSKVVVV
>Mature_478_residues
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGAPEDHKKRADDTRAIAKELGVHVAVLGDLQGPKIRISTFKD
NRKVMLVLGQAYTLDADLAKGEGDENQVGIDYKELPQDVSLGDILMLDDGRVQLRVESVEGNKVHTIVTVAGPLSNNKGI
NKQGGGLSAAALTEKDKQDIITAAEIKVDYLAVSFPRSGADLNYARDLARQAGSNALIVSKVERAEAVATDEAMDDVIMA
SDAVMVARGDLGVEIGDPALVAVQKRLISRSRQLNKPVITATQMMESMISSPMPTRAEVMDVANAVLDGTDAVMLSAETA
AGDFPEETVKAMAEVCLGAESHPSVQVSKHRLDQQFTTIEETIALSTMYAANHLEGIKAIIALTESGATPQMMSRISSAL
PIFALSRHESTLAKMALYRGVQPVYFDSTAHAPEAVAQKALETLSQAGYLVKGDMAMMTKGDAMETVGGTNTSKVVVV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=495, Percent_Identity=35.959595959596, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI33286420, Length=495, Percent_Identity=35.959595959596, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=35.959595959596, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=33.8709677419355, Blast_Score=238, Evalue=7e-63,
Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=33.8709677419355, Blast_Score=238, Evalue=8e-63,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=35.2941176470588, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=35.2941176470588, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=33.1914893617021, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=33.1914893617021, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=32.7102803738318, Blast_Score=103, Evalue=5e-22,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=71.2788259958071, Blast_Score=679, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=480, Percent_Identity=37.5, Blast_Score=285, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI17544584, Length=487, Percent_Identity=36.1396303901437, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17506831, Length=493, Percent_Identity=34.8884381338742, Blast_Score=232, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI71984413, Length=493, Percent_Identity=34.8884381338742, Blast_Score=232, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI17506829, Length=493, Percent_Identity=34.8884381338742, Blast_Score=231, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI71984406, Length=493, Percent_Identity=34.8884381338742, Blast_Score=231, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6319279, Length=474, Percent_Identity=36.0759493670886, Blast_Score=258, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6324923, Length=433, Percent_Identity=34.4110854503464, Blast_Score=220, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24648964, Length=501, Percent_Identity=33.9321357285429, Blast_Score=249, Evalue=4e-66,
Organism=Drosophila melanogaster, GI28571814, Length=501, Percent_Identity=33.9321357285429, Blast_Score=248, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24648966, Length=449, Percent_Identity=34.9665924276169, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24581235, Length=496, Percent_Identity=30.8467741935484, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI24646914, Length=271, Percent_Identity=37.2693726937269, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51069; Mature: 51069

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGAPEDHKKRADDTRAIAKELGV
CCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHCCHHHHHHHHHHCE
HVAVLGDLQGPKIRISTFKDNRKVMLVLGQAYTLDADLAKGEGDENQVGIDYKELPQDVS
EEEEEECCCCCEEEEEEECCCCEEEEEEECEEEECCHHHCCCCCCCCCCCCHHHCCCCCC
LGDILMLDDGRVQLRVESVEGNKVHTIVTVAGPLSNNKGINKQGGGLSAAALTEKDKQDI
CCCEEEEECCEEEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCEEEHHHCCCCHHHC
ITAAEIKVDYLAVSFPRSGADLNYARDLARQAGSNALIVSKVERAEAVATDEAMDDVIMA
EEEEEEEEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHC
SDAVMVARGDLGVEIGDPALVAVQKRLISRSRQLNKPVITATQMMESMISSPMPTRAEVM
CCEEEEEECCCCEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHH
DVANAVLDGTDAVMLSAETAAGDFPEETVKAMAEVCLGAESHPSVQVSKHRLDQQFTTIE
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHH
ETIALSTMYAANHLEGIKAIIALTESGATPQMMSRISSALPIFALSRHESTLAKMALYRG
HHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHC
VQPVYFDSTAHAPEAVAQKALETLSQAGYLVKGDMAMMTKGDAMETVGGTNTSKVVVV
CCCEEECCCCCCHHHHHHHHHHHHHHCCCEEECCEEEEECCCCHHHCCCCCCCEEEEC
>Mature Secondary Structure
MFRRTKIVTTLGPATDRDDNLRRIIAAGANVVRLNFSHGAPEDHKKRADDTRAIAKELGV
CCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHCCHHHHHHHHHHCE
HVAVLGDLQGPKIRISTFKDNRKVMLVLGQAYTLDADLAKGEGDENQVGIDYKELPQDVS
EEEEEECCCCCEEEEEEECCCCEEEEEEECEEEECCHHHCCCCCCCCCCCCHHHCCCCCC
LGDILMLDDGRVQLRVESVEGNKVHTIVTVAGPLSNNKGINKQGGGLSAAALTEKDKQDI
CCCEEEEECCEEEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCEEEHHHCCCCHHHC
ITAAEIKVDYLAVSFPRSGADLNYARDLARQAGSNALIVSKVERAEAVATDEAMDDVIMA
EEEEEEEEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHC
SDAVMVARGDLGVEIGDPALVAVQKRLISRSRQLNKPVITATQMMESMISSPMPTRAEVM
CCEEEEEECCCCEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHH
DVANAVLDGTDAVMLSAETAAGDFPEETVKAMAEVCLGAESHPSVQVSKHRLDQQFTTIE
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHH
ETIALSTMYAANHLEGIKAIIALTESGATPQMMSRISSALPIFALSRHESTLAKMALYRG
HHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHC
VQPVYFDSTAHAPEAVAQKALETLSQAGYLVKGDMAMMTKGDAMETVGGTNTSKVVVV
CCCEEECCCCCCHHHHHHHHHHHHHHCCCEEECCEEEEECCCCHHHCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]