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The map label for this gene is 157962116

Identifier: 157962116

GI number: 157962116

Start: 2782955

End: 2784538

Strand: Direct

Name: 157962116

Synonym: Spea_2295

Alternate gene names: NA

Gene position: 2782955-2784538 (Clockwise)

Preceding gene: 157962115

Following gene: 157962119

Centisome position: 53.78

GC content: 48.86

Gene sequence:

>1584_bases
ATGTACACCAGTCAAACAACCCAAGCGGCAACCGATGCTATCGAGTGGGCGTTAACTCATGGTATGGCACTAAAAACCAC
ACCTTACTCGGCAACCCATACCGCCTTCAGCTTTACGCCGACACCTATTAGCAAGGCGAGGTATCAACAGCTGGTGGACT
CAGTCGGTTTGCTTGGCAAGCTGGTTCATTTTGTATCTGAAGATCACCGATTCTTAAACCAAGCGATCGCCCCTATCACC
AAAGGCGATCCCTTCTTTAAGGCCCTGCTGGAGATGCATCAAGCTATTCATCAAGCAAGCAATCAAACGAGCGTTCAAGT
GAGCAATCAAGCGCCCCAACAATCAATTGATCAAGGCGTGTCGTCAGAAAGTCGTGGTGGTGACGCCAAACGCTTACCGC
TACTCATTATGCGCAGTGACTTTATGGACGATGCGGCCTTAGGACCAAAGCTTATCGAGTTCAACGGCATTGCCGCAGGT
ATGGGGCCATTCGGACAACGAGTCCATGAACTACATAAATACCTGCAGCTTCAAGCCGCCGATACGTTTAACTTGTGGTC
AGCCAGCCCAAACAGCAAGCTGGTAGACAATCACGCATTAGAGGGATTGGCTCAAGGCATAGCTAATGCCACATTTAAAA
TTAAGCGCGAGTTTACAAAAAACGACGCTTCGACAGGTTTTAAAAACAGTCCTCCCGTGTTCCTAATGGTAGTGCAAGCC
AATGAGGATAATGTTTACGATCAACACCTGTTAGAGCATGTACTGCAAGCAAAAGGCATTAAAACGGTAAGACGCACTTT
TCGCGAACTGCACGACAAACTCACAACCGGTAAAGATGATAGATTACTGCTCGAGGGTGTCGGCCCCATCGATACCGTGT
ATCTGCGCGCTGGTTATCAATATTGTGATTATCACGCCAATGACATCATCAGCCGTGTATGCTGCGAAGCACTGATGCAA
ACCCGTATTTTTATTGAAAAACACCGAGTGGCGGTTAACGCTACCGTTAGCCAGCAACTGGCCACCAGCAAGCGGGTGCA
GATGTTACTGTCTTCTATGGCACCTGACGCCCTCACCCGTTTTGGTTTAACTTTAGATGAGGCCAAAACCGTTAAAGGCT
TACTTGGCGAGATGCGCGCAGTCACTCAAGACAGCGCTACATTGATTGCCCACAGTAATACTGACGAATGGGTGCTAAAA
AACCAAGGTGAAGGTGGCGGCCACTGCATATTTGGCGAAGACATTCTGCCAAAGCTTGCCGAGCTTAAACCAAGCGAATA
TCAGGCCTGGTCACTGATGCGAAGACTACATCCAACCGCGAGAGCTACGGCCGCGTTAACCGTACGCAAAGGTGAGCTCC
ACAGAGTCGACGATTTAATTAGCGAAATCGGCATGTTCACCGTACATATCGACGGCGGGGCAGCGATAAACATAATAGAT
GATAGCGAGCAGGATACGAACAAACAGCTTAACGCCAAGCAAGCTTACGCCGGTTACTTGATAAGAAGTAAATCAGCGCG
CACCACAGAAGGCGGCGTTCACAGCGGTCAGGGCGTATTGGATTCGTTAGCTTTTAGTGATTAG

Upstream 100 bases:

>100_bases
CTCTATTTCGAACAAAATCGAAATAACAATTGACACAGCAGCCAGCTACAACTACATTTCCAGATACATGGAATTGTCAA
AAAGTAAAAGGTGCTAAATC

Downstream 100 bases:

>100_bases
ATTTTAGTGGCTAGCTTTTAGTGACTAGCTTTTAGTGACTAGATTCTACTAACTAGATTTTAGTAATTGAGGCTAATCGT
TAGAACTGCAGATTAAGTTT

Product: glutathione synthase

Products: ADP; phosphate; glutathione

Alternate protein names: Glutathione Synthetase

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MYTSQTTQAATDAIEWALTHGMALKTTPYSATHTAFSFTPTPISKARYQQLVDSVGLLGKLVHFVSEDHRFLNQAIAPIT
KGDPFFKALLEMHQAIHQASNQTSVQVSNQAPQQSIDQGVSSESRGGDAKRLPLLIMRSDFMDDAALGPKLIEFNGIAAG
MGPFGQRVHELHKYLQLQAADTFNLWSASPNSKLVDNHALEGLAQGIANATFKIKREFTKNDASTGFKNSPPVFLMVVQA
NEDNVYDQHLLEHVLQAKGIKTVRRTFRELHDKLTTGKDDRLLLEGVGPIDTVYLRAGYQYCDYHANDIISRVCCEALMQ
TRIFIEKHRVAVNATVSQQLATSKRVQMLLSSMAPDALTRFGLTLDEAKTVKGLLGEMRAVTQDSATLIAHSNTDEWVLK
NQGEGGGHCIFGEDILPKLAELKPSEYQAWSLMRRLHPTARATAALTVRKGELHRVDDLISEIGMFTVHIDGGAAINIID
DSEQDTNKQLNAKQAYAGYLIRSKSARTTEGGVHSGQGVLDSLAFSD

Sequences:

>Translated_527_residues
MYTSQTTQAATDAIEWALTHGMALKTTPYSATHTAFSFTPTPISKARYQQLVDSVGLLGKLVHFVSEDHRFLNQAIAPIT
KGDPFFKALLEMHQAIHQASNQTSVQVSNQAPQQSIDQGVSSESRGGDAKRLPLLIMRSDFMDDAALGPKLIEFNGIAAG
MGPFGQRVHELHKYLQLQAADTFNLWSASPNSKLVDNHALEGLAQGIANATFKIKREFTKNDASTGFKNSPPVFLMVVQA
NEDNVYDQHLLEHVLQAKGIKTVRRTFRELHDKLTTGKDDRLLLEGVGPIDTVYLRAGYQYCDYHANDIISRVCCEALMQ
TRIFIEKHRVAVNATVSQQLATSKRVQMLLSSMAPDALTRFGLTLDEAKTVKGLLGEMRAVTQDSATLIAHSNTDEWVLK
NQGEGGGHCIFGEDILPKLAELKPSEYQAWSLMRRLHPTARATAALTVRKGELHRVDDLISEIGMFTVHIDGGAAINIID
DSEQDTNKQLNAKQAYAGYLIRSKSARTTEGGVHSGQGVLDSLAFSD
>Mature_527_residues
MYTSQTTQAATDAIEWALTHGMALKTTPYSATHTAFSFTPTPISKARYQQLVDSVGLLGKLVHFVSEDHRFLNQAIAPIT
KGDPFFKALLEMHQAIHQASNQTSVQVSNQAPQQSIDQGVSSESRGGDAKRLPLLIMRSDFMDDAALGPKLIEFNGIAAG
MGPFGQRVHELHKYLQLQAADTFNLWSASPNSKLVDNHALEGLAQGIANATFKIKREFTKNDASTGFKNSPPVFLMVVQA
NEDNVYDQHLLEHVLQAKGIKTVRRTFRELHDKLTTGKDDRLLLEGVGPIDTVYLRAGYQYCDYHANDIISRVCCEALMQ
TRIFIEKHRVAVNATVSQQLATSKRVQMLLSSMAPDALTRFGLTLDEAKTVKGLLGEMRAVTQDSATLIAHSNTDEWVLK
NQGEGGGHCIFGEDILPKLAELKPSEYQAWSLMRRLHPTARATAALTVRKGELHRVDDLISEIGMFTVHIDGGAAINIID
DSEQDTNKQLNAKQAYAGYLIRSKSARTTEGGVHSGQGVLDSLAFSD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4504169, Length=538, Percent_Identity=26.9516728624535, Blast_Score=149, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17535239, Length=529, Percent_Identity=24.5746691871456, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324524, Length=544, Percent_Identity=24.8161764705882, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI281361051, Length=531, Percent_Identity=25.4237288135593, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24642900, Length=531, Percent_Identity=25.4237288135593, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI281361044, Length=531, Percent_Identity=25.4237288135593, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI281361034, Length=531, Percent_Identity=25.4237288135593, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI281361053, Length=581, Percent_Identity=25.473321858864, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI281376915, Length=581, Percent_Identity=25.473321858864, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI281361042, Length=581, Percent_Identity=25.473321858864, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI281361040, Length=581, Percent_Identity=25.473321858864, Blast_Score=132, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.3

Molecular weight: Translated: 57943; Mature: 57943

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTSQTTQAATDAIEWALTHGMALKTTPYSATHTAFSFTPTPISKARYQQLVDSVGLLGK
CCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
LVHFVSEDHRFLNQAIAPITKGDPFFKALLEMHQAIHQASNQTSVQVSNQAPQQSIDQGV
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCC
SSESRGGDAKRLPLLIMRSDFMDDAALGPKLIEFNGIAAGMGPFGQRVHELHKYLQLQAA
CCCCCCCCCCCCCEEEEECCCCCCHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
DTFNLWSASPNSKLVDNHALEGLAQGIANATFKIKREFTKNDASTGFKNSPPVFLMVVQA
CCEECCCCCCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHCCCCCCCCCCCCCEEEEEEEC
NEDNVYDQHLLEHVLQAKGIKTVRRTFRELHDKLTTGKDDRLLLEGVGPIDTVYLRAGYQ
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHEEHHHCCCH
YCDYHANDIISRVCCEALMQTRIFIEKHRVAVNATVSQQLATSKRVQMLLSSMAPDALTR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHH
FGLTLDEAKTVKGLLGEMRAVTQDSATLIAHSNTDEWVLKNQGEGGGHCIFGEDILPKLA
HCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCEEEECHHHHHHHH
ELKPSEYQAWSLMRRLHPTARATAALTVRKGELHRVDDLISEIGMFTVHIDGGAAINIID
HCCCCHHHHHHHHHHHCCHHHHHEEEEEECCCHHHHHHHHHHCCEEEEEECCCEEEEEEC
DSEQDTNKQLNAKQAYAGYLIRSKSARTTEGGVHSGQGVLDSLAFSD
CCCCCHHHHCCHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MYTSQTTQAATDAIEWALTHGMALKTTPYSATHTAFSFTPTPISKARYQQLVDSVGLLGK
CCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
LVHFVSEDHRFLNQAIAPITKGDPFFKALLEMHQAIHQASNQTSVQVSNQAPQQSIDQGV
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCC
SSESRGGDAKRLPLLIMRSDFMDDAALGPKLIEFNGIAAGMGPFGQRVHELHKYLQLQAA
CCCCCCCCCCCCCEEEEECCCCCCHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
DTFNLWSASPNSKLVDNHALEGLAQGIANATFKIKREFTKNDASTGFKNSPPVFLMVVQA
CCEECCCCCCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHCCCCCCCCCCCCCEEEEEEEC
NEDNVYDQHLLEHVLQAKGIKTVRRTFRELHDKLTTGKDDRLLLEGVGPIDTVYLRAGYQ
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHEEHHHCCCH
YCDYHANDIISRVCCEALMQTRIFIEKHRVAVNATVSQQLATSKRVQMLLSSMAPDALTR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHH
FGLTLDEAKTVKGLLGEMRAVTQDSATLIAHSNTDEWVLKNQGEGGGHCIFGEDILPKLA
HCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCEEEECHHHHHHHH
ELKPSEYQAWSLMRRLHPTARATAALTVRKGELHRVDDLISEIGMFTVHIDGGAAINIID
HCCCCHHHHHHHHHHHCCHHHHHEEEEEECCCHHHHHHHHHHCCEEEEEECCCEEEEEEC
DSEQDTNKQLNAKQAYAGYLIRSKSARTTEGGVHSGQGVLDSLAFSD
CCCCCHHHHCCHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; gamma-L-glutamyl-L-cysteine; glycine

Specific reaction: ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA