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The map label for this gene is norM [H]

Identifier: 157962089

GI number: 157962089

Start: 2745103

End: 2746461

Strand: Direct

Name: norM [H]

Synonym: Spea_2268

Alternate gene names: 157962089

Gene position: 2745103-2746461 (Clockwise)

Preceding gene: 157962087

Following gene: 157962090

Centisome position: 53.05

GC content: 45.4

Gene sequence:

>1359_bases
ATGAACCATTCTGGCTTTCAAGCCAAACGCTTAGTCCAATTAGCGCTACCAGTGCTTATTGCCCAAGTCACACAAACCAT
GATGGGCTTTATCGATACCGTTATGGCTGGGCGTGTTAGCGCCGTCGATATGGCCGCAGTAGCGATCAGTGCCAGTCTTT
GGCTACCGGCATTATTGTTTGTTCAAGGCTTATTAATGGCCTTTACACCAGTATTTGCTCACCATCATGGTGCCGATAAT
CAAAAAGCGATTCAACCATTAGCTTTTCAAGCTGGTTATATTGCCATAGTTGGTAGCCTTGTTGTGGTGCTTATCCTCTC
ATTTGCAGAGAATATTTTCACCATGATGGATCTTGAACCTGAGATGGCAAGACTTGGCGTTGGTTACCTTAATGGCTTCA
TGTGGGGCGTACCCGCGTTCGTACTTTATCAAGTATTAAGAGGCTGTAGCGAAGGGATCTCTTATACCTTACCGACTATG
GTGATTGGTTTTGTCGGCCTAGCAGTCAATATTCCCGCAAACTACATCTTTATATATGGTCATTTTGGTGTGCCAGCAAT
GGGCGGTGCAGGCTGTGGTATAGCAACGGCGCTCGTGTTCTGGGCCATGTTAATCGCCATGGTGATCTACATGCAGCTGC
ATAAAAAATTTAAAGAGCTGGCACCGTTCAAATCTTTTCACATGCCAGACCTAAAAGTCATGTTTGATATGACAAAACAC
GGCTTCCCTATCGCTATGGCGCTGTTCTTTGAAGTGAGTTTATTTGCAATCATTGCGCTGCTATTGGCCCCTTTAGGCGC
CACAGTGGTAGCAGGACATCAGATTGCACTTAACTTTTCATCGATTGTATTTATGTTACCGCTGTCGTTGGGTATCGCGG
TTTCAATCCGCGTAGGCTATTACTTAGGCCAAGACAAGCCTGAAATCGCAGCGCTAGTCACTAAGCTAGCACTAATGATT
TCCTTTTCGTTAGCAGCATGCACTGCTGTGATTACTATTTTATTTAGAAACGAAATAGCGCTGCTTTATAATGATAATCC
AGAGGTTATCACCCTGGCGGGTAGCTTGATGTTCTTTGCCGCTATCTATCAATTATCGGACTCAATACAAGTGGTTACCG
CAGGTGCGCTACGTGGTTACAAAGATACCCGCAGCGCATTTTATATCACACTGGTTTCGTATTGGGGTGTAGGTATGACC
TTAGGCTATACCTTGGCTCGCACCGATATTATTGTGCCGCACATGGGCGCGCAAGGTTTCTGGATTGGTTTGATTGCCGG
TTTAACCGCTGCTGCAATTATGTTTGCAGCTCGACTACGCTACATTCAAAAACAGGGCGTTGTGCACTCAGCAATATAA

Upstream 100 bases:

>100_bases
GTTTAAGCCTGATTTCTTATCTATCGCCAACACTTCGCATGTTGCTTGAACTATACCTGTAAACATCTTATCTCTCGTTA
GTATATTAAGGGCTGTACCC

Downstream 100 bases:

>100_bases
TTGAGTGTCGATAGACTTCGCGAAACAAAAAAAGGCTAACTCTTTTCGAGTTGGCCTTTTTATTTTCAATGTCCCGTCCT
GAGATAAGTTGACACTTTGG

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MNHSGFQAKRLVQLALPVLIAQVTQTMMGFIDTVMAGRVSAVDMAAVAISASLWLPALLFVQGLLMAFTPVFAHHHGADN
QKAIQPLAFQAGYIAIVGSLVVVLILSFAENIFTMMDLEPEMARLGVGYLNGFMWGVPAFVLYQVLRGCSEGISYTLPTM
VIGFVGLAVNIPANYIFIYGHFGVPAMGGAGCGIATALVFWAMLIAMVIYMQLHKKFKELAPFKSFHMPDLKVMFDMTKH
GFPIAMALFFEVSLFAIIALLLAPLGATVVAGHQIALNFSSIVFMLPLSLGIAVSIRVGYYLGQDKPEIAALVTKLALMI
SFSLAACTAVITILFRNEIALLYNDNPEVITLAGSLMFFAAIYQLSDSIQVVTAGALRGYKDTRSAFYITLVSYWGVGMT
LGYTLARTDIIVPHMGAQGFWIGLIAGLTAAAIMFAARLRYIQKQGVVHSAI

Sequences:

>Translated_452_residues
MNHSGFQAKRLVQLALPVLIAQVTQTMMGFIDTVMAGRVSAVDMAAVAISASLWLPALLFVQGLLMAFTPVFAHHHGADN
QKAIQPLAFQAGYIAIVGSLVVVLILSFAENIFTMMDLEPEMARLGVGYLNGFMWGVPAFVLYQVLRGCSEGISYTLPTM
VIGFVGLAVNIPANYIFIYGHFGVPAMGGAGCGIATALVFWAMLIAMVIYMQLHKKFKELAPFKSFHMPDLKVMFDMTKH
GFPIAMALFFEVSLFAIIALLLAPLGATVVAGHQIALNFSSIVFMLPLSLGIAVSIRVGYYLGQDKPEIAALVTKLALMI
SFSLAACTAVITILFRNEIALLYNDNPEVITLAGSLMFFAAIYQLSDSIQVVTAGALRGYKDTRSAFYITLVSYWGVGMT
LGYTLARTDIIVPHMGAQGFWIGLIAGLTAAAIMFAARLRYIQKQGVVHSAI
>Mature_452_residues
MNHSGFQAKRLVQLALPVLIAQVTQTMMGFIDTVMAGRVSAVDMAAVAISASLWLPALLFVQGLLMAFTPVFAHHHGADN
QKAIQPLAFQAGYIAIVGSLVVVLILSFAENIFTMMDLEPEMARLGVGYLNGFMWGVPAFVLYQVLRGCSEGISYTLPTM
VIGFVGLAVNIPANYIFIYGHFGVPAMGGAGCGIATALVFWAMLIAMVIYMQLHKKFKELAPFKSFHMPDLKVMFDMTKH
GFPIAMALFFEVSLFAIIALLLAPLGATVVAGHQIALNFSSIVFMLPLSLGIAVSIRVGYYLGQDKPEIAALVTKLALMI
SFSLAACTAVITILFRNEIALLYNDNPEVITLAGSLMFFAAIYQLSDSIQVVTAGALRGYKDTRSAFYITLVSYWGVGMT
LGYTLARTDIIVPHMGAQGFWIGLIAGLTAAAIMFAARLRYIQKQGVVHSAI

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI153792564, Length=451, Percent_Identity=23.7250554323725, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI22907060, Length=433, Percent_Identity=22.1709006928406, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI38261960, Length=487, Percent_Identity=22.7926078028747, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI48994890, Length=439, Percent_Identity=52.8473804100228, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI226510954, Length=446, Percent_Identity=23.7668161434978, Blast_Score=99, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6321821, Length=402, Percent_Identity=21.3930348258706, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320545, Length=432, Percent_Identity=19.9074074074074, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 48836; Mature: 48836

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]