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The map label for this gene is ald [H]

Identifier: 157962056

GI number: 157962056

Start: 2708284

End: 2709399

Strand: Direct

Name: ald [H]

Synonym: Spea_2235

Alternate gene names: 157962056

Gene position: 2708284-2709399 (Clockwise)

Preceding gene: 157962048

Following gene: 157962061

Centisome position: 52.34

GC content: 44.53

Gene sequence:

>1116_bases
ATGATTATAGGTGTTCCAAAAGAAATCAAAAACCACGAATATCGTGTTGGTATGGTTCCATCAAGTGTTCGTGAATTGAC
GTCACGTGGTCATGAGGTTTTTATTGAAACAAATGCTGGTTCAGGCATTGGTTTTGTAGATCAAGATTACATCGAAGTTG
GTGCAAAAATACTGGATACCGCTGCAGAGGTATTTGCTAAATCAGAAATGATTGTAAAAGTAAAAGAACCCCAAGCCGTT
GAACGTGCAATGTTGCGTGAAGACCAGCTACTGTTTACCTATCTTCATCTAGCGCCGGATCTGCCTCAAACAGAAGATCT
CGTTAAAAGTGGCTCTGTGTGTATAGCGTACGAGACAGTTACTGATGATCGCGGTACCCTTCCACTACTCGCACCTATGT
CAGAAGTTGCTGGACGCATGTCAATTCAAGCCGGTGCTATGGCATTAGAAAAATCTATGGGTGGTCGCGGCATGCTACTC
GGCGGCGTACCCGGTGTAGAGCCTGCTAAAGTCGTTATCATTGGTGGCGGCATGGTAGGTACCAACGCTGCGCAGATGGC
TGTTGGTCTAGGTGCTGACGTTGTTATCTTAGATAGAAGCATCGATGCGCTTCGTCGTCTCAATGCACAATTCGATAATA
AAGTTAAAGCTATCTACTCAACGGCAGACGCTATCGAAAAACACGTACTAGAAGCTGACCTTGTTATCGGTGGTGTACTG
GTCCCAGGCGCCGCAGCACCAAAGCTTGTGACGAAAGAACACATCAAACGTATGAAGCCTGGTTCAGCAATTGTAGACGT
TGCAATCGACCAAGGTGGTTGTATTGAGACATCACACGCTACAACTCACCAAGATCCAACTTATATCGTAGATGATGTTG
TACATTACTGTGTGGCAAACATGCCTGGCGCTGTAGCCCGTACATCAACATTCGCTCTTAATAATGCTACTTTGCCGTAT
ATCATTAAGCTAGCTCAATTAGGCTATAGAGAAGCGCTATTACAAGATAAGCACTTACTTAATGGCCTTAATGTTATGCA
CGGTAAGATCACTTGCGCCGAAGTTGCTGAAGCATTGAACCTTGAGTTTGTTGATCCAACTATCCTGCTTAAGTAA

Upstream 100 bases:

>100_bases
ATACTATACTAGATTTCCCTGAAACATTCACGTTCAGGCAATATAAAATAACAATTACACCCATAGAGAATGGGTTTTTA
TGCGAATTTGAGGCTCGAAG

Downstream 100 bases:

>100_bases
AACGACTCATAAAAAAAAGGAGCCTATATGGCTCCCTTTTTTTGAACTCTTGCTAATGACTTAGCTTATCGTAGAAAAAC
TGTAGCCCTTTCCCCTAACT

Product: alanine dehydrogenase

Products: NA

Alternate protein names: 40 kDa antigen; TB43 [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MIIGVPKEIKNHEYRVGMVPSSVRELTSRGHEVFIETNAGSGIGFVDQDYIEVGAKILDTAAEVFAKSEMIVKVKEPQAV
ERAMLREDQLLFTYLHLAPDLPQTEDLVKSGSVCIAYETVTDDRGTLPLLAPMSEVAGRMSIQAGAMALEKSMGGRGMLL
GGVPGVEPAKVVIIGGGMVGTNAAQMAVGLGADVVILDRSIDALRRLNAQFDNKVKAIYSTADAIEKHVLEADLVIGGVL
VPGAAAPKLVTKEHIKRMKPGSAIVDVAIDQGGCIETSHATTHQDPTYIVDDVVHYCVANMPGAVARTSTFALNNATLPY
IIKLAQLGYREALLQDKHLLNGLNVMHGKITCAEVAEALNLEFVDPTILLK

Sequences:

>Translated_371_residues
MIIGVPKEIKNHEYRVGMVPSSVRELTSRGHEVFIETNAGSGIGFVDQDYIEVGAKILDTAAEVFAKSEMIVKVKEPQAV
ERAMLREDQLLFTYLHLAPDLPQTEDLVKSGSVCIAYETVTDDRGTLPLLAPMSEVAGRMSIQAGAMALEKSMGGRGMLL
GGVPGVEPAKVVIIGGGMVGTNAAQMAVGLGADVVILDRSIDALRRLNAQFDNKVKAIYSTADAIEKHVLEADLVIGGVL
VPGAAAPKLVTKEHIKRMKPGSAIVDVAIDQGGCIETSHATTHQDPTYIVDDVVHYCVANMPGAVARTSTFALNNATLPY
IIKLAQLGYREALLQDKHLLNGLNVMHGKITCAEVAEALNLEFVDPTILLK
>Mature_371_residues
MIIGVPKEIKNHEYRVGMVPSSVRELTSRGHEVFIETNAGSGIGFVDQDYIEVGAKILDTAAEVFAKSEMIVKVKEPQAV
ERAMLREDQLLFTYLHLAPDLPQTEDLVKSGSVCIAYETVTDDRGTLPLLAPMSEVAGRMSIQAGAMALEKSMGGRGMLL
GGVPGVEPAKVVIIGGGMVGTNAAQMAVGLGADVVILDRSIDALRRLNAQFDNKVKAIYSTADAIEKHVLEADLVIGGVL
VPGAAAPKLVTKEHIKRMKPGSAIVDVAIDQGGCIETSHATTHQDPTYIVDDVVHYCVANMPGAVARTSTFALNNATLPY
IIKLAQLGYREALLQDKHLLNGLNVMHGKITCAEVAEALNLEFVDPTILLK

Specific function: May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=347, Percent_Identity=29.971181556196, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI122939153, Length=347, Percent_Identity=29.971181556196, Blast_Score=115, Evalue=6e-26,
Organism=Escherichia coli, GI1787887, Length=298, Percent_Identity=29.8657718120805, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17550456, Length=348, Percent_Identity=29.3103448275862, Blast_Score=124, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 39630; Mature: 39630

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGVPKEIKNHEYRVGMVPSSVRELTSRGHEVFIETNAGSGIGFVDQDYIEVGAKILDT
CEECCCHHHCCCCEEEECCCHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
AAEVFAKSEMIVKVKEPQAVERAMLREDQLLFTYLHLAPDLPQTEDLVKSGSVCIAYETV
HHHHHHCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHCCCEEEEEEEE
TDDRGTLPLLAPMSEVAGRMSIQAGAMALEKSMGGRGMLLGGVPGVEPAKVVIIGGGMVG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCEEC
TNAAQMAVGLGADVVILDRSIDALRRLNAQFDNKVKAIYSTADAIEKHVLEADLVIGGVL
CCHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEEEE
VPGAAAPKLVTKEHIKRMKPGSAIVDVAIDQGGCIETSHATTHQDPTYIVDDVVHYCVAN
CCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHC
MPGAVARTSTFALNNATLPYIIKLAQLGYREALLQDKHLLNGLNVMHGKITCAEVAEALN
CCCCHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEHHHHHHHHC
LEFVDPTILLK
CEEECCEEEEC
>Mature Secondary Structure
MIIGVPKEIKNHEYRVGMVPSSVRELTSRGHEVFIETNAGSGIGFVDQDYIEVGAKILDT
CEECCCHHHCCCCEEEECCCHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
AAEVFAKSEMIVKVKEPQAVERAMLREDQLLFTYLHLAPDLPQTEDLVKSGSVCIAYETV
HHHHHHCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHCCCEEEEEEEE
TDDRGTLPLLAPMSEVAGRMSIQAGAMALEKSMGGRGMLLGGVPGVEPAKVVIIGGGMVG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCEEC
TNAAQMAVGLGADVVILDRSIDALRRLNAQFDNKVKAIYSTADAIEKHVLEADLVIGGVL
CCHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEEEE
VPGAAAPKLVTKEHIKRMKPGSAIVDVAIDQGGCIETSHATTHQDPTYIVDDVVHYCVAN
CCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHC
MPGAVARTSTFALNNATLPYIIKLAQLGYREALLQDKHLLNGLNVMHGKITCAEVAEALN
CCCCHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEHHHHHHHHC
LEFVDPTILLK
CEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1587598; 9634230; 12218036; 7822223 [H]