Click here to switch to the map view.

The map label for this gene is ttcA [H]

Identifier: 157962045

GI number: 157962045

Start: 2696062

End: 2696997

Strand: Direct

Name: ttcA [H]

Synonym: Spea_2224

Alternate gene names: 157962045

Gene position: 2696062-2696997 (Clockwise)

Preceding gene: 157962044

Following gene: 157962048

Centisome position: 52.1

GC content: 45.62

Gene sequence:

>936_bases
ATGTCCGAAGAATTAACTCCACAACAAGTCACTCGTATGACTAAACTGCAGAAGCGTTTACGCACTGAAGTTGGCCGTGC
AATTGGTGATTACAACATGATTGAAGAAGGTGACCGAGTTATGTGTTGCCTCTCGGGTGGCAAAGACAGCTACGCCATGC
TCGATATCTTGTTGAACCTTCAGCAACGCGCTCCTATTAAATTCGAAATCGTTGCGGTTAACTTAGATCAGAAGCAACCC
GGTTTCCCTGAAGATATTCTGCCAGCATACTTAGATACTTTAGGCGTGGCTTACCATATCCTAGAAAAAGATACTTACTC
TATCGTTAAAGATAAGATCCCAGAAGGCAAGACTACCTGCTCACTATGCTCGCGCCTACGCCGCGGTACCTTATATGGCT
TTGCGCAAAAGATTGGTGCGACCAAAATTGCTCTAGGCCATCATAGAGATGACATCATCGAGACCATGTTCTTGAATATG
TTCTTTGCCGGTAAGATGAAGGCAATGCCACCTAAGTTACTATCAGATGACGGTGCCAACATGGTTATTCGCCCGTTAGC
CTATGCTCGCGAAAAAGATATTGCTGAGTATGCAGCGCTTAAAGGCTTCCCAATTATTCCTTGTAACCTTTGTGGGTCAC
AAGAAAACCTCAAACGTGCAGCAGTCAAAGAGATGTTAGTACAGTGGGACAAGCAGCATCCAGGTCGTATTGAAACCATC
TTCACTGCGATGCAAAATACTGCCCCATCACAGGGTGTAGACAGAGAGCAGTTTGACTTCTTATCACTGCAGCGTGACCC
AGATGCGCCAATGAGTGGTCAAGTTGCCGAATCTGATCTGCCTGCATTCGATTTCGTTGATGTATCGAATAACGGTCATA
TCAACCTTGATGCCGCTGCGCGAATCGATGTTGTCAGTACTTATACGCCAGAATAG

Upstream 100 bases:

>100_bases
TCACCATTAGAAGAGGTCTAACCGCTTTCTAAGCTTTACCCGAAGCCATTAAGCTGGAATAATACGCGCCCTGAACTATT
GTGTACTAGGCGCGTTTTTT

Downstream 100 bases:

>100_bases
CCTATTGATGAATAAAGATAAAAAAAGCCCTGATTAACAGGGCTTTTTTGTAGCAGTATTTCGGCTCTTAGTAACTTATT
TCACTGTCCAAGGACTGATA

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSEELTPQQVTRMTKLQKRLRTEVGRAIGDYNMIEEGDRVMCCLSGGKDSYAMLDILLNLQQRAPIKFEIVAVNLDQKQP
GFPEDILPAYLDTLGVAYHILEKDTYSIVKDKIPEGKTTCSLCSRLRRGTLYGFAQKIGATKIALGHHRDDIIETMFLNM
FFAGKMKAMPPKLLSDDGANMVIRPLAYAREKDIAEYAALKGFPIIPCNLCGSQENLKRAAVKEMLVQWDKQHPGRIETI
FTAMQNTAPSQGVDREQFDFLSLQRDPDAPMSGQVAESDLPAFDFVDVSNNGHINLDAAARIDVVSTYTPE

Sequences:

>Translated_311_residues
MSEELTPQQVTRMTKLQKRLRTEVGRAIGDYNMIEEGDRVMCCLSGGKDSYAMLDILLNLQQRAPIKFEIVAVNLDQKQP
GFPEDILPAYLDTLGVAYHILEKDTYSIVKDKIPEGKTTCSLCSRLRRGTLYGFAQKIGATKIALGHHRDDIIETMFLNM
FFAGKMKAMPPKLLSDDGANMVIRPLAYAREKDIAEYAALKGFPIIPCNLCGSQENLKRAAVKEMLVQWDKQHPGRIETI
FTAMQNTAPSQGVDREQFDFLSLQRDPDAPMSGQVAESDLPAFDFVDVSNNGHINLDAAARIDVVSTYTPE
>Mature_310_residues
SEELTPQQVTRMTKLQKRLRTEVGRAIGDYNMIEEGDRVMCCLSGGKDSYAMLDILLNLQQRAPIKFEIVAVNLDQKQPG
FPEDILPAYLDTLGVAYHILEKDTYSIVKDKIPEGKTTCSLCSRLRRGTLYGFAQKIGATKIALGHHRDDIIETMFLNMF
FAGKMKAMPPKLLSDDGANMVIRPLAYAREKDIAEYAALKGFPIIPCNLCGSQENLKRAAVKEMLVQWDKQHPGRIETIF
TAMQNTAPSQGVDREQFDFLSLQRDPDAPMSGQVAESDLPAFDFVDVSNNGHINLDAAARIDVVSTYTPE

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=275, Percent_Identity=65.0909090909091, Blast_Score=387, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI193211090, Length=224, Percent_Identity=33.9285714285714, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17540090, Length=248, Percent_Identity=25.4032258064516, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24651972, Length=234, Percent_Identity=27.3504273504274, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 34723; Mature: 34592

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEELTPQQVTRMTKLQKRLRTEVGRAIGDYNMIEEGDRVMCCLSGGKDSYAMLDILLNL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHH
QQRAPIKFEIVAVNLDQKQPGFPEDILPAYLDTLGVAYHILEKDTYSIVKDKIPEGKTTC
HHCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
SLCSRLRRGTLYGFAQKIGATKIALGHHRDDIIETMFLNMFFAGKMKAMPPKLLSDDGAN
HHHHHHHHCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCC
MVIRPLAYAREKDIAEYAALKGFPIIPCNLCGSQENLKRAAVKEMLVQWDKQHPGRIETI
EEEHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
FTAMQNTAPSQGVDREQFDFLSLQRDPDAPMSGQVAESDLPAFDFVDVSNNGHINLDAAA
HHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCC
RIDVVSTYTPE
EEEEEECCCCC
>Mature Secondary Structure 
SEELTPQQVTRMTKLQKRLRTEVGRAIGDYNMIEEGDRVMCCLSGGKDSYAMLDILLNL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHH
QQRAPIKFEIVAVNLDQKQPGFPEDILPAYLDTLGVAYHILEKDTYSIVKDKIPEGKTTC
HHCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
SLCSRLRRGTLYGFAQKIGATKIALGHHRDDIIETMFLNMFFAGKMKAMPPKLLSDDGAN
HHHHHHHHCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCC
MVIRPLAYAREKDIAEYAALKGFPIIPCNLCGSQENLKRAAVKEMLVQWDKQHPGRIETI
EEEHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
FTAMQNTAPSQGVDREQFDFLSLQRDPDAPMSGQVAESDLPAFDFVDVSNNGHINLDAAA
HHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCC
RIDVVSTYTPE
EEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA