Click here to switch to the map view.

The map label for this gene is mltB [H]

Identifier: 157961727

GI number: 157961727

Start: 2314764

End: 2315954

Strand: Direct

Name: mltB [H]

Synonym: Spea_1904

Alternate gene names: 157961727

Gene position: 2314764-2315954 (Clockwise)

Preceding gene: 157961724

Following gene: 157961728

Centisome position: 44.73

GC content: 47.94

Gene sequence:

>1191_bases
TTGTTAAAATCAATCCCCCTGGCTACAGCGCTAATCCTATCTACTTCTGCTGCTTATGCGGCAGACGAGCAGCCTAATTG
GGCTAGCTGCGTGGCCGAGCTACAACAAACGGCAAAAGCAGAAGGTCTATCTCAACAGACCATAGAGACCACGTTGGCCA
ATGTTAAGTACGTGCCGAGAGTGATTGAACTCGATCAAAAACAACCTGAATTTAGCACGTCTTTTGAAAATTATTTTAGT
AAGCGGGTAACAGATTGGCGAGTGGAGCAGGGGCGTAAGCTCTATAGTGAGCACAAAGTATTGTTACAAAAACTGGCAAA
AGAATATGGCGTGCCGCCTCAGTACCTGCTTGCATTCTGGGGACTAGAAACTAATTTTGGCTCTTATAAGGGCAAGATGT
CGGTATTAGATTCATTGGCAACCTTGGCTTGCGATCCAAGGCGCAGTCGTTATTTCACCACTGAGTTGATGCAAGCATTA
AAGCTTAAAGAGCGATACAACTTTGATGAAGCTGATATGGTCGGGTCTTGGGCTGGTGCCATGGGACACACTCAATTTAT
GCCGTCGGCCTATGCAAAATACGCTGTGGATGGCAATGGTGATGGTAAAGCAGACCTGTGGAACAGTACCGAAGATGCTT
TAACCTCTGCGGCTAACTTCTTACAAAATCTAGGTTGGAAACGCAATGAACGTTGGGGGCGAGAAGTCACCTTACCGGAA
AACTTCTCATACGCGCACTTAGGCAAAGCCGAGTCTCAGCCGCTTACTCGTTGGGCAGAGCTGGGGATCACACAAACCAA
TGGTGCACCGTTAAGTACGCCAGATATGCAAGCTGCACTTTACCTGCCGTCGGGTCATACGGGCCCCGCATTTTTAGGTT
ATGACAACTTTGATGTGATCATGCGCTGGAATCGCTCAACCTTTTACGCCATCGCAGTAGGTCACCTTGCCGACAGAATC
AATGGCGCTGTAGCGCTAAAAGTGACACCACCTAAGATGCCAAACTTGAGTCGTGCTCGAGTTAAAGAGCTGCAAAACCA
GCTGAACCATTTAGGTTATGATGTGGGTAAGGCCGATGGCATTTTGGGCTCAAAGTCGGTTAAAGGCTTACAGAGCTTCC
AAAAGAGTCAGGGGCTTGTTGCCGACGGCTACCCGGATGAAGCGACGTTTAAGGCGCTAAAGGTTAAGTAA

Upstream 100 bases:

>100_bases
ACAACGTACATGACTCAGGTTAGATTTAGTGAGTTTAAGGAAAGTTTAATCAAACCGCGTCATTTTATACGTTACAGTCA
TTCATTTTGGGAGTCTTCAA

Downstream 100 bases:

>100_bases
ACTAATTACAGACAAGCAAGCTTAAAGTGAGTGATATAATTTTGACTCATTAGATAACACTAGAGGGTTCAGGCGAGTTT
TGTTAAGCTGCGGCCACTAA

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MLKSIPLATALILSTSAAYAADEQPNWASCVAELQQTAKAEGLSQQTIETTLANVKYVPRVIELDQKQPEFSTSFENYFS
KRVTDWRVEQGRKLYSEHKVLLQKLAKEYGVPPQYLLAFWGLETNFGSYKGKMSVLDSLATLACDPRRSRYFTTELMQAL
KLKERYNFDEADMVGSWAGAMGHTQFMPSAYAKYAVDGNGDGKADLWNSTEDALTSAANFLQNLGWKRNERWGREVTLPE
NFSYAHLGKAESQPLTRWAELGITQTNGAPLSTPDMQAALYLPSGHTGPAFLGYDNFDVIMRWNRSTFYAIAVGHLADRI
NGAVALKVTPPKMPNLSRARVKELQNQLNHLGYDVGKADGILGSKSVKGLQSFQKSQGLVADGYPDEATFKALKVK

Sequences:

>Translated_396_residues
MLKSIPLATALILSTSAAYAADEQPNWASCVAELQQTAKAEGLSQQTIETTLANVKYVPRVIELDQKQPEFSTSFENYFS
KRVTDWRVEQGRKLYSEHKVLLQKLAKEYGVPPQYLLAFWGLETNFGSYKGKMSVLDSLATLACDPRRSRYFTTELMQAL
KLKERYNFDEADMVGSWAGAMGHTQFMPSAYAKYAVDGNGDGKADLWNSTEDALTSAANFLQNLGWKRNERWGREVTLPE
NFSYAHLGKAESQPLTRWAELGITQTNGAPLSTPDMQAALYLPSGHTGPAFLGYDNFDVIMRWNRSTFYAIAVGHLADRI
NGAVALKVTPPKMPNLSRARVKELQNQLNHLGYDVGKADGILGSKSVKGLQSFQKSQGLVADGYPDEATFKALKVK
>Mature_396_residues
MLKSIPLATALILSTSAAYAADEQPNWASCVAELQQTAKAEGLSQQTIETTLANVKYVPRVIELDQKQPEFSTSFENYFS
KRVTDWRVEQGRKLYSEHKVLLQKLAKEYGVPPQYLLAFWGLETNFGSYKGKMSVLDSLATLACDPRRSRYFTTELMQAL
KLKERYNFDEADMVGSWAGAMGHTQFMPSAYAKYAVDGNGDGKADLWNSTEDALTSAANFLQNLGWKRNERWGREVTLPE
NFSYAHLGKAESQPLTRWAELGITQTNGAPLSTPDMQAALYLPSGHTGPAFLGYDNFDVIMRWNRSTFYAIAVGHLADRI
NGAVALKVTPPKMPNLSRARVKELQNQLNHLGYDVGKADGILGSKSVKGLQSFQKSQGLVADGYPDEATFKALKVK

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=302, Percent_Identity=31.4569536423841, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 43976; Mature: 43976

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSIPLATALILSTSAAYAADEQPNWASCVAELQQTAKAEGLSQQTIETTLANVKYVPR
CCCCCCHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VIELDQKQPEFSTSFENYFSKRVTDWRVEQGRKLYSEHKVLLQKLAKEYGVPPQYLLAFW
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
GLETNFGSYKGKMSVLDSLATLACDPRRSRYFTTELMQALKLKERYNFDEADMVGSWAGA
CCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
MGHTQFMPSAYAKYAVDGNGDGKADLWNSTEDALTSAANFLQNLGWKRNERWGREVTLPE
CCCHHHCCHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCCC
NFSYAHLGKAESQPLTRWAELGITQTNGAPLSTPDMQAALYLPSGHTGPAFLGYDNFDVI
CCCHHHCCCCCCCHHHHHHHCCCEECCCCCCCCCCCCEEEECCCCCCCCEEECCCCCEEE
MRWNRSTFYAIAVGHLADRINGAVALKVTPPKMPNLSRARVKELQNQLNHLGYDVGKADG
EEECCCEEEEEHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
ILGSKSVKGLQSFQKSQGLVADGYPDEATFKALKVK
CCCCHHHHHHHHHHHHCCCEECCCCCHHHCEEEECC
>Mature Secondary Structure
MLKSIPLATALILSTSAAYAADEQPNWASCVAELQQTAKAEGLSQQTIETTLANVKYVPR
CCCCCCHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VIELDQKQPEFSTSFENYFSKRVTDWRVEQGRKLYSEHKVLLQKLAKEYGVPPQYLLAFW
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
GLETNFGSYKGKMSVLDSLATLACDPRRSRYFTTELMQALKLKERYNFDEADMVGSWAGA
CCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
MGHTQFMPSAYAKYAVDGNGDGKADLWNSTEDALTSAANFLQNLGWKRNERWGREVTLPE
CCCHHHCCHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCCC
NFSYAHLGKAESQPLTRWAELGITQTNGAPLSTPDMQAALYLPSGHTGPAFLGYDNFDVI
CCCHHHCCCCCCCHHHHHHHCCCEECCCCCCCCCCCCEEEECCCCCCCCEEECCCCCEEE
MRWNRSTFYAIAVGHLADRINGAVALKVTPPKMPNLSRARVKELQNQLNHLGYDVGKADG
EEECCCEEEEEHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
ILGSKSVKGLQSFQKSQGLVADGYPDEATFKALKVK
CCCCHHHHHHHHHHHHCCCEECCCCCHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]