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The map label for this gene is nirD [H]

Identifier: 157961636

GI number: 157961636

Start: 2214696

End: 2215076

Strand: Direct

Name: nirD [H]

Synonym: Spea_1812

Alternate gene names: 157961636

Gene position: 2214696-2215076 (Clockwise)

Preceding gene: 157961635

Following gene: 157961637

Centisome position: 42.8

GC content: 45.41

Gene sequence:

>381_bases
ATGAGCTGGGTAGCATTATGTGATGAAGCTAAGTTGCCTAAGGGGCAAGGTGTAGCTGCTTGGCTTGATAATCGAAAGAT
CGCTTTGTTTAATTTGGGCGAGAAAGGCATATTTGCCCTCGATAATATTGACCCCGCAACTGGCGTTGCGGTGCTTTCAC
GGGGACTCGTTTGTGAACTTAACGATGAACTGTATGTAGCGTCACCTATTCATAAACAGCATTATCATCTTTCCACAGGT
CAGTGTTTAGAGAACGATGGATTATCGGTTATCTGCTATGAGTTAAAAAAAGAGCAAGGTAGGGTCCTCGCAAAATTGCC
TTTTCAAGCTGCATGCGCGTCAGGCAGTGCTCTGTCCACCCCAGTAAAAGGAGCAAGGTAA

Upstream 100 bases:

>100_bases
CAATCCAATCCCAAGTCGATACATTTCATGCGATTTCTAAGCCTCAGTATGGCGCTGGGGATATCGCAATCAATATTATT
GATGAGCAGGAGGTCATGGT

Downstream 100 bases:

>100_bases
CATGAGTTCTCAAGCATTCAATCTTTTCTCCTTTAGCGGAAAAATGAAAGTCATGCACTTAAGCTGGATGGCATTTTTTA
TCACCTTTATGGTCTGGTTT

Product: nitrite reductase (NAD(P)H) small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MSWVALCDEAKLPKGQGVAAWLDNRKIALFNLGEKGIFALDNIDPATGVAVLSRGLVCELNDELYVASPIHKQHYHLSTG
QCLENDGLSVICYELKKEQGRVLAKLPFQAACASGSALSTPVKGAR

Sequences:

>Translated_126_residues
MSWVALCDEAKLPKGQGVAAWLDNRKIALFNLGEKGIFALDNIDPATGVAVLSRGLVCELNDELYVASPIHKQHYHLSTG
QCLENDGLSVICYELKKEQGRVLAKLPFQAACASGSALSTPVKGAR
>Mature_125_residues
SWVALCDEAKLPKGQGVAAWLDNRKIALFNLGEKGIFALDNIDPATGVAVLSRGLVCELNDELYVASPIHKQHYHLSTGQ
CLENDGLSVICYELKKEQGRVLAKLPFQAACASGSALSTPVKGAR

Specific function: Required for activity of the reductase [H]

COG id: COG2146

COG function: function code PR; Ferredoxin subunits of nitrite reductase and ring-hydroxylating dioxygenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis nasE [H]

Homologues:

Organism=Escherichia coli, GI1789766, Length=101, Percent_Identity=39.6039603960396, Blast_Score=74, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR017941 [H]

Pfam domain/function: NA

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 13525; Mature: 13394

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWVALCDEAKLPKGQGVAAWLDNRKIALFNLGEKGIFALDNIDPATGVAVLSRGLVCEL
CCCEECCCCCCCCCCCCEEEEECCCEEEEEECCCCCEEEECCCCCHHHHHHHHCCCEEEE
NDELYVASPIHKQHYHLSTGQCLENDGLSVICYELKKEQGRVLAKLPFQAACASGSALST
CCCEEEECCCCCCEEECCCCCEECCCCCEEEEEEECCCCCCEEEECCCHHHCCCCCCCCC
PVKGAR
CCCCCC
>Mature Secondary Structure 
SWVALCDEAKLPKGQGVAAWLDNRKIALFNLGEKGIFALDNIDPATGVAVLSRGLVCEL
CCEECCCCCCCCCCCCEEEEECCCEEEEEECCCCCEEEECCCCCHHHHHHHHCCCEEEE
NDELYVASPIHKQHYHLSTGQCLENDGLSVICYELKKEQGRVLAKLPFQAACASGSALST
CCCEEEECCCCCCEEECCCCCEECCCCCEEEEEEECCCCCCEEEECCCHHHCCCCCCCCC
PVKGAR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]