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The map label for this gene is gcvA [H]

Identifier: 157961029

GI number: 157961029

Start: 1462229

End: 1463170

Strand: Direct

Name: gcvA [H]

Synonym: Spea_1201

Alternate gene names: 157961029

Gene position: 1462229-1463170 (Clockwise)

Preceding gene: 157961028

Following gene: 157961030

Centisome position: 28.26

GC content: 46.5

Gene sequence:

>942_bases
ATGCGTCGCCTTCCGCCATTAAACCCTTTGAGAGCCTTCGAGTCTGCCGCTAGACATGAGCATTTCTCTCGTGCTGCAGA
TGAACTCTGCGTCACCCATAGCGCGATTAGTCATCAAGTGAAGACTTTAGAAGATGCCTTAGGGATTAGGTTGTTTGAGA
AGCAAGGCCGTAACGCAAAATTGACTCAGTCTGGAGCAAATCTATTACCAGCAATTCAAGAAGCATTCGATATCATAGGT
CAAGCCTGTGAAAAGGCGATTGAGCCAGGACTCAGTGGTCGCTTGGTCATTTCCGCTCCCCCCGAACTCTCCTCTAAGTG
GCTTATTAAACAGATTGGCGATTTTGCCATTCGTTATCCTTCAATCGACGTCAGTCTATTCGTGCACTCTAGTGACGAGA
CTAACATTAACCCACAGGCCGATCTTTCGATTATCTATGGTGCAGGAGATGCCGATTGGAATCGCTATTGGGTCACTCCG
ATTAAAAGAATGGAGTTTTTCCCTGTCTGCAGCCCCAAGCTGCTCGAATCGGAAAATAATCTGGGGGGCGCGGCTAAGAA
AGAGGATTGTCGCTCTCTAGACTTGAGTGTGCAGCGCCTATTACACGACGATCAAGATGGTAAAACTTGGGCTACTTGGC
TTGGAGCTCATGCGCCGCATATCTTAAATACACCGCAAAACTTAAACTTTGCCCATGCTGGTCTGTCTCTAGAAGCGGCA
ATCGCTGGATATGGGGTTGCTATCGGTGATAATTTTACCGCCAGTACTGAGCTAGAGAGTGGTAAGTTGGTTAGGCCTTT
TGAGCACAGTGTTCCTTCATTGGGTAACTATTATTTGGTTGCAGAGAAACGAAAAAAAGGCGATGTAAAACTCATGACTT
TTATTGATTGGCTGCTGGCTCAAATCAATACTAAATTCAGCACCGTTAGCCTCCAGGATTAG

Upstream 100 bases:

>100_bases
GGGATTTTTTATTAATTGAGCAATGATAAATTGTTGCTGTTAATTTAATCTGTTCTGGCATAGTCTAATTTAGTCACTTT
ATTAACCAGATTATGAGCTT

Downstream 100 bases:

>100_bases
TCGTTTAGTCAGTTGAATGCTAACTCCTCCCATCAATAAATCCCGTTATTTCCAATCGCAAAATACGGTTAGTTTTTCTC
TGAACTTTTGCAGTAGAGAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRRLPPLNPLRAFESAARHEHFSRAADELCVTHSAISHQVKTLEDALGIRLFEKQGRNAKLTQSGANLLPAIQEAFDIIG
QACEKAIEPGLSGRLVISAPPELSSKWLIKQIGDFAIRYPSIDVSLFVHSSDETNINPQADLSIIYGAGDADWNRYWVTP
IKRMEFFPVCSPKLLESENNLGGAAKKEDCRSLDLSVQRLLHDDQDGKTWATWLGAHAPHILNTPQNLNFAHAGLSLEAA
IAGYGVAIGDNFTASTELESGKLVRPFEHSVPSLGNYYLVAEKRKKGDVKLMTFIDWLLAQINTKFSTVSLQD

Sequences:

>Translated_313_residues
MRRLPPLNPLRAFESAARHEHFSRAADELCVTHSAISHQVKTLEDALGIRLFEKQGRNAKLTQSGANLLPAIQEAFDIIG
QACEKAIEPGLSGRLVISAPPELSSKWLIKQIGDFAIRYPSIDVSLFVHSSDETNINPQADLSIIYGAGDADWNRYWVTP
IKRMEFFPVCSPKLLESENNLGGAAKKEDCRSLDLSVQRLLHDDQDGKTWATWLGAHAPHILNTPQNLNFAHAGLSLEAA
IAGYGVAIGDNFTASTELESGKLVRPFEHSVPSLGNYYLVAEKRKKGDVKLMTFIDWLLAQINTKFSTVSLQD
>Mature_313_residues
MRRLPPLNPLRAFESAARHEHFSRAADELCVTHSAISHQVKTLEDALGIRLFEKQGRNAKLTQSGANLLPAIQEAFDIIG
QACEKAIEPGLSGRLVISAPPELSSKWLIKQIGDFAIRYPSIDVSLFVHSSDETNINPQADLSIIYGAGDADWNRYWVTP
IKRMEFFPVCSPKLLESENNLGGAAKKEDCRSLDLSVQRLLHDDQDGKTWATWLGAHAPHILNTPQNLNFAHAGLSLEAA
IAGYGVAIGDNFTASTELESGKLVRPFEHSVPSLGNYYLVAEKRKKGDVKLMTFIDWLLAQINTKFSTVSLQD

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=302, Percent_Identity=37.4172185430464, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1788706, Length=308, Percent_Identity=30.8441558441558, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786448, Length=302, Percent_Identity=32.4503311258278, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787128, Length=117, Percent_Identity=37.6068376068376, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI145693193, Length=303, Percent_Identity=23.1023102310231, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI87082132, Length=141, Percent_Identity=31.2056737588652, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI157672245, Length=117, Percent_Identity=35.042735042735, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787589, Length=291, Percent_Identity=23.0240549828179, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34573; Mature: 34573

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLPPLNPLRAFESAARHEHFSRAADELCVTHSAISHQVKTLEDALGIRLFEKQGRNAK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEHHHCCCCCE
LTQSGANLLPAIQEAFDIIGQACEKAIEPGLSGRLVISAPPELSSKWLIKQIGDFAIRYP
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHEEECC
SIDVSLFVHSSDETNINPQADLSIIYGAGDADWNRYWVTPIKRMEFFPVCSPKLLESENN
CCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEECHHHHCEECCCCCHHHHCCCCC
LGGAAKKEDCRSLDLSVQRLLHDDQDGKTWATWLGAHAPHILNTPQNLNFAHAGLSLEAA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHCCCCCCCCEEECCCEEEEH
IAGYGVAIGDNFTASTELESGKLVRPFEHSVPSLGNYYLVAEKRKKGDVKLMTFIDWLLA
HHCCEEEECCCCCCCCCCCCCCEECCHHHCCCCCCCEEEEEECCCCCCEEHHHHHHHHHH
QINTKFSTVSLQD
HHCCCEEEEEECC
>Mature Secondary Structure
MRRLPPLNPLRAFESAARHEHFSRAADELCVTHSAISHQVKTLEDALGIRLFEKQGRNAK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEHHHCCCCCE
LTQSGANLLPAIQEAFDIIGQACEKAIEPGLSGRLVISAPPELSSKWLIKQIGDFAIRYP
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHEEECC
SIDVSLFVHSSDETNINPQADLSIIYGAGDADWNRYWVTPIKRMEFFPVCSPKLLESENN
CCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEECHHHHCEECCCCCHHHHCCCCC
LGGAAKKEDCRSLDLSVQRLLHDDQDGKTWATWLGAHAPHILNTPQNLNFAHAGLSLEAA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHCCCCCCCCEEECCCEEEEH
IAGYGVAIGDNFTASTELESGKLVRPFEHSVPSLGNYYLVAEKRKKGDVKLMTFIDWLLA
HHCCEEEECCCCCCCCCCCCCCEECCHHHCCCCCCCEEEEEECCCCCCEEHHHHHHHHHH
QINTKFSTVSLQD
HHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]