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The map label for this gene is ispD

Identifier: 157961015

GI number: 157961015

Start: 1441729

End: 1442424

Strand: Direct

Name: ispD

Synonym: Spea_1187

Alternate gene names: 157961015

Gene position: 1441729-1442424 (Clockwise)

Preceding gene: 157961014

Following gene: 157961016

Centisome position: 27.86

GC content: 49.28

Gene sequence:

>696_bases
ATGAGCCAAACACCCAAGCACATAATCTCGATAGTCCCCGCTGCTGGTATAGGCAGTCGTATGGGCGCAGAAATTCCAAA
GCAATATTTACAACTTAATGAACAAAGCATTTTAGGGCATACGCTGGACTGCTTATTATCGCATCCTAGTATCGAAAAGG
TGATCGTGGCGCTTAACCCTGAAGATGAGTTCTTTGCGCGCTTGCCTCAAGCTCAGCATGCCAAACTACAGGCTGTAATT
GGTGGTAAAGAGCGCGCAGACTCAGTGCTGTCGGCTTTAACTGTGGCACCAACGGATGCCTGGGCGCTAGTGCATGATGC
GGCGCGTCCGTGCTTAAACCATCAAGACATAGATAAGTTAATCGAGTCAGCATCTAGCTTTCCACAAGGCGCTATTCTAG
GAGCCCCTGTGCGTGACACCATGAAGCGTAGTAATGCACAGGGCTTAATTACCGAAACGGTTTGCCGCGAAAAGCTGTGG
CATGCACTCACACCGCAGTTTTTTCCTGTGCAGAATCTAAAGCAAAATCTCAGTAATGCTTTAGCCGCAGGCGCATTAAT
TACCGATGAGGCTTCGGCTATGGAGTGGGCTGGTGTTGCTCCAGGCATTGTTGCTGGTCGCGCCGACAATATTAAGGTGA
CCCATCCAGACGATCTGCAGCTAGCCTCGCTATTTCTTAAAAGTGCAGCTCAATAG

Upstream 100 bases:

>100_bases
CTTCTTTCGCGTCGTTGGAGGCGAACGTGGGGGCGTACCTGAGAACTGATTTTAGCTTAACCAATCTTAGAAAGATGCCG
CTATTACATGGAGCTCTATC

Downstream 100 bases:

>100_bases
GCTAACTTAGAAACTAATTCGCTAAGAATTTAATAGATAAATAGGAACACCGATGAAAATTAGAATTGGTCATGGCTTTG
ATGTTCACAAGTTTGGTGGC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSQTPKHIISIVPAAGIGSRMGAEIPKQYLQLNEQSILGHTLDCLLSHPSIEKVIVALNPEDEFFARLPQAQHAKLQAVI
GGKERADSVLSALTVAPTDAWALVHDAARPCLNHQDIDKLIESASSFPQGAILGAPVRDTMKRSNAQGLITETVCREKLW
HALTPQFFPVQNLKQNLSNALAAGALITDEASAMEWAGVAPGIVAGRADNIKVTHPDDLQLASLFLKSAAQ

Sequences:

>Translated_231_residues
MSQTPKHIISIVPAAGIGSRMGAEIPKQYLQLNEQSILGHTLDCLLSHPSIEKVIVALNPEDEFFARLPQAQHAKLQAVI
GGKERADSVLSALTVAPTDAWALVHDAARPCLNHQDIDKLIESASSFPQGAILGAPVRDTMKRSNAQGLITETVCREKLW
HALTPQFFPVQNLKQNLSNALAAGALITDEASAMEWAGVAPGIVAGRADNIKVTHPDDLQLASLFLKSAAQ
>Mature_230_residues
SQTPKHIISIVPAAGIGSRMGAEIPKQYLQLNEQSILGHTLDCLLSHPSIEKVIVALNPEDEFFARLPQAQHAKLQAVIG
GKERADSVLSALTVAPTDAWALVHDAARPCLNHQDIDKLIESASSFPQGAILGAPVRDTMKRSNAQGLITETVCREKLWH
ALTPQFFPVQNLKQNLSNALAAGALITDEASAMEWAGVAPGIVAGRADNIKVTHPDDLQLASLFLKSAAQ

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Escherichia coli, GI1789104, Length=227, Percent_Identity=51.9823788546256, Blast_Score=237, Evalue=4e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_SHEPA (A8H1S7)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001501049.1
- ProteinModelPortal:   A8H1S7
- SMR:   A8H1S7
- STRING:   A8H1S7
- GeneID:   5661586
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_1187
- NMPDR:   fig|398579.3.peg.1036
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- ProtClustDB:   PRK00155
- BioCyc:   SPEA398579:SPEA_1187-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 24743; Mature: 24611

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTPKHIISIVPAAGIGSRMGAEIPKQYLQLNEQSILGHTLDCLLSHPSIEKVIVALNP
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHEEEEEECC
EDEFFARLPQAQHAKLQAVIGGKERADSVLSALTVAPTDAWALVHDAARPCLNHQDIDKL
CHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHH
IESASSFPQGAILGAPVRDTMKRSNAQGLITETVCREKLWHALTPQFFPVQNLKQNLSNA
HHHHHCCCCCCEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LAAGALITDEASAMEWAGVAPGIVAGRADNIKVTHPDDLQLASLFLKSAAQ
HHHHHHCCCCCHHHHHCCCCCCCEECCCCCEEEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SQTPKHIISIVPAAGIGSRMGAEIPKQYLQLNEQSILGHTLDCLLSHPSIEKVIVALNP
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHEEEEEECC
EDEFFARLPQAQHAKLQAVIGGKERADSVLSALTVAPTDAWALVHDAARPCLNHQDIDKL
CHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHH
IESASSFPQGAILGAPVRDTMKRSNAQGLITETVCREKLWHALTPQFFPVQNLKQNLSNA
HHHHHCCCCCCEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LAAGALITDEASAMEWAGVAPGIVAGRADNIKVTHPDDLQLASLFLKSAAQ
HHHHHHCCCCCHHHHHCCCCCCCEECCCCCEEEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA