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The map label for this gene is ribE [H]

Identifier: 157960991

GI number: 157960991

Start: 1408714

End: 1409379

Strand: Direct

Name: ribE [H]

Synonym: Spea_1163

Alternate gene names: 157960991

Gene position: 1408714-1409379 (Clockwise)

Preceding gene: 157960990

Following gene: 157960992

Centisome position: 27.22

GC content: 48.05

Gene sequence:

>666_bases
ATGTTTACTGGGATTATTGAATCAGTCGGACACCTACGAAAAGTTGAGCGTCGCGGCGATGATATTCGCTTAACGGTAGC
CAGTGGTAAACTGGATCTGTCAGACGTAAAACTGGGTGACAGTATCGCCACCAATGGTGTGTGCCTAACAGTGGTTGAGC
TGATGAGCGACGGTTATGTGGCAGATATTTCTGCAGAAACTGTCACTTTGACGGGCTTCTCTCATTATCAAGTAGGAAAA
GCCGTTAACCTTGAGAAGGCCGTGACCCCTACAACTCGTTTAGGCGGGCATATGGTCAGTGGTCACGTAGATGGTATCGC
TCATGTGGATGATAGACAGTTTCGTGGCCAAGCCATCGAATTTTGGCTAAGCCCACCTGAGGAACTTGGCCGTTATATCG
CCCATAAAGGTTCGATTACCATCGATGGTGTGAGTTTGACGGTTAATGAGGTGCAAGGTCATCGTTTTAGACTGACTATC
GTGCCGCACACCGCAGGCGAAACCACTTTAGTGAATCTGAAAGCCGGTGATAGCGTGAATATTGAAGTGGACTTGATTGC
ACGTCACTTAGAAAGGTTGATGAGCTACGGCAGCCAAGAGCAAGACAGCGCTCCTAAGTCGGAAGTGACGATGGAACTGT
TAGCTCGCTCAGGATTTTTACGATAA

Upstream 100 bases:

>100_bases
TGATTTTCAAGCCATGTCAGAGGTGCCAAAGATTAACCTAATCGATGAGCGCAAAGTGGGGCCAGACACCCGCTTAACGT
TAAGAGTGAGTAATTAATTT

Downstream 100 bases:

>100_bases
ACACTGGCTTAAGATTAGTTTGTCAGTAAAATTTGTCTTATATAAAGAATAGAATAATCATTACATTTACCGCTGATTTT
CATCGTGTCTTTAACAGGCG

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MFTGIIESVGHLRKVERRGDDIRLTVASGKLDLSDVKLGDSIATNGVCLTVVELMSDGYVADISAETVTLTGFSHYQVGK
AVNLEKAVTPTTRLGGHMVSGHVDGIAHVDDRQFRGQAIEFWLSPPEELGRYIAHKGSITIDGVSLTVNEVQGHRFRLTI
VPHTAGETTLVNLKAGDSVNIEVDLIARHLERLMSYGSQEQDSAPKSEVTMELLARSGFLR

Sequences:

>Translated_221_residues
MFTGIIESVGHLRKVERRGDDIRLTVASGKLDLSDVKLGDSIATNGVCLTVVELMSDGYVADISAETVTLTGFSHYQVGK
AVNLEKAVTPTTRLGGHMVSGHVDGIAHVDDRQFRGQAIEFWLSPPEELGRYIAHKGSITIDGVSLTVNEVQGHRFRLTI
VPHTAGETTLVNLKAGDSVNIEVDLIARHLERLMSYGSQEQDSAPKSEVTMELLARSGFLR
>Mature_221_residues
MFTGIIESVGHLRKVERRGDDIRLTVASGKLDLSDVKLGDSIATNGVCLTVVELMSDGYVADISAETVTLTGFSHYQVGK
AVNLEKAVTPTTRLGGHMVSGHVDGIAHVDDRQFRGQAIEFWLSPPEELGRYIAHKGSITIDGVSLTVNEVQGHRFRLTI
VPHTAGETTLVNLKAGDSVNIEVDLIARHLERLMSYGSQEQDSAPKSEVTMELLARSGFLR

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=185, Percent_Identity=35.6756756756757, Blast_Score=107, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6319733, Length=218, Percent_Identity=38.0733944954128, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 24009; Mature: 24009

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIIESVGHLRKVERRGDDIRLTVASGKLDLSDVKLGDSIATNGVCLTVVELMSDGYV
CCCHHHHHHHHHHHHHHCCCEEEEEEECCCEEECCEECCCCCCCCCHHHHHHHHHCCCEE
ADISAETVTLTGFSHYQVGKAVNLEKAVTPTTRLGGHMVSGHVDGIAHVDDRQFRGQAIE
EECCCCEEEEECCCCEECCCCCCCHHHCCCCHHCCCEEEECCCCCEEECCCCHHCCCEEE
FWLSPPEELGRYIAHKGSITIDGVSLTVNEVQGHRFRLTIVPHTAGETTLVNLKAGDSVN
EECCCHHHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEEEEECCCCCEEEEEEECCCCEE
IEVDLIARHLERLMSYGSQEQDSAPKSEVTMELLARSGFLR
EEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFTGIIESVGHLRKVERRGDDIRLTVASGKLDLSDVKLGDSIATNGVCLTVVELMSDGYV
CCCHHHHHHHHHHHHHHCCCEEEEEEECCCEEECCEECCCCCCCCCHHHHHHHHHCCCEE
ADISAETVTLTGFSHYQVGKAVNLEKAVTPTTRLGGHMVSGHVDGIAHVDDRQFRGQAIE
EECCCCEEEEECCCCEECCCCCCCHHHCCCCHHCCCEEEECCCCCEEECCCCHHCCCEEE
FWLSPPEELGRYIAHKGSITIDGVSLTVNEVQGHRFRLTIVPHTAGETTLVNLKAGDSVN
EECCCHHHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEEEEECCCCCEEEEEEECCCCEE
IEVDLIARHLERLMSYGSQEQDSAPKSEVTMELLARSGFLR
EEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144477 [H]