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The map label for this gene is rihB [H]

Identifier: 157960838

GI number: 157960838

Start: 1228104

End: 1229078

Strand: Direct

Name: rihB [H]

Synonym: Spea_1010

Alternate gene names: 157960838

Gene position: 1228104-1229078 (Clockwise)

Preceding gene: 157960831

Following gene: 157960842

Centisome position: 23.73

GC content: 42.87

Gene sequence:

>975_bases
ATGATAAAAAAGATTATATTAGATACAGATCCAGGAATCGATGACGTTATGGCTATCATGTTTGCTGAGGCTCATCCAGA
AGTAGAACTGAGGGCAATTACGACGATTTTTGGTAATGTTACAATAGATACCGCCACTCACAATGCTCTTTACCTGAAAG
AGAAATATCAATTAAAAGCAGATGTCGTTAAAGGTGCCGATAAGCCATTAGTAAGGCCACCTGTTGGGCCTTCAGTTGTG
GTTCATGGTGAGAGCGGTTTTGGTGATGTCGAAGTGCCAAACGAAGTTGAGGCTGTGGCTGATCCACGTAGTGCGCATCA
ATATATTATTGATGCGGTAAAAGCTGAACCAAATGAGATAACCTTAGTTGCTATTGGTCCTTTAACAAACCTAGCATTGG
CGCTACAAGCCGAACCTGAAATAGTCAACCTTGTCAAAGAAGTGGTGATTATGGGCGGCGCGTTTGGAGTCAACGATCAT
CGCGGAAATGTAACACCTTATGCAGAGGCTAATATCCATGATGACCCCCATGCAGCAGACATAGTGTTTAGTGCATCATG
GCCTGTGGTCATTATAGGTTTAGATGTTACCGAGCAGAGTTTTTTTACAGGTGACTATTTAGAGCAACTTAGAGATGACG
CTGGAGAACTCGGACAGTTTATTTGGGATGTTAGTCGATTTTACCTCAAGTTTTACAGAGACAAAGTGGGCTTAGATGCC
TGCCATGTTCATGACCCTTCAGCCATCGCTTATGTGATAAAACCTGATTTATTTACCTTAAGGGAAGGACCTGTGCGTGT
TGTCACTGAAGGACCTGCTGAGGGAATGACTGTTCAGAAGTTTGATGGTAGAAAGTATTTACACGATGACTGGGAAAACC
ACACTCCTCAGCGAGTGGGAGTAGCGGTTAAAGACGCTGAATTATTGAAGCTCTATAGACAATGTATTGTTGATTACGCT
CATAAGGTTGGGTGA

Upstream 100 bases:

>100_bases
AACGAACTATTTTGTTCTATTTTGGGTGCAAGTTTGAAGGCTTATCACAATCACTGGCGAGTTCATCTTGTATATTGAGT
TAAAAAAGGATGATAAACCA

Downstream 100 bases:

>100_bases
GTTTCTTAAACTAACATGGGTAGGTTGCCTCATTATAATGAGGCTTTTACTAAGCCGGTAGGAACTCCCTACTGGCCTAT
TACGTTTTGTGTTTTATTTT

Product: inosine/uridine-preferring nucleoside hydrolase

Products: NA

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MIKKIILDTDPGIDDVMAIMFAEAHPEVELRAITTIFGNVTIDTATHNALYLKEKYQLKADVVKGADKPLVRPPVGPSVV
VHGESGFGDVEVPNEVEAVADPRSAHQYIIDAVKAEPNEITLVAIGPLTNLALALQAEPEIVNLVKEVVIMGGAFGVNDH
RGNVTPYAEANIHDDPHAADIVFSASWPVVIIGLDVTEQSFFTGDYLEQLRDDAGELGQFIWDVSRFYLKFYRDKVGLDA
CHVHDPSAIAYVIKPDLFTLREGPVRVVTEGPAEGMTVQKFDGRKYLHDDWENHTPQRVGVAVKDAELLKLYRQCIVDYA
HKVG

Sequences:

>Translated_324_residues
MIKKIILDTDPGIDDVMAIMFAEAHPEVELRAITTIFGNVTIDTATHNALYLKEKYQLKADVVKGADKPLVRPPVGPSVV
VHGESGFGDVEVPNEVEAVADPRSAHQYIIDAVKAEPNEITLVAIGPLTNLALALQAEPEIVNLVKEVVIMGGAFGVNDH
RGNVTPYAEANIHDDPHAADIVFSASWPVVIIGLDVTEQSFFTGDYLEQLRDDAGELGQFIWDVSRFYLKFYRDKVGLDA
CHVHDPSAIAYVIKPDLFTLREGPVRVVTEGPAEGMTVQKFDGRKYLHDDWENHTPQRVGVAVKDAELLKLYRQCIVDYA
HKVG
>Mature_324_residues
MIKKIILDTDPGIDDVMAIMFAEAHPEVELRAITTIFGNVTIDTATHNALYLKEKYQLKADVVKGADKPLVRPPVGPSVV
VHGESGFGDVEVPNEVEAVADPRSAHQYIIDAVKAEPNEITLVAIGPLTNLALALQAEPEIVNLVKEVVIMGGAFGVNDH
RGNVTPYAEANIHDDPHAADIVFSASWPVVIIGLDVTEQSFFTGDYLEQLRDDAGELGQFIWDVSRFYLKFYRDKVGLDA
CHVHDPSAIAYVIKPDLFTLREGPVRVVTEGPAEGMTVQKFDGRKYLHDDWENHTPQRVGVAVKDAELLKLYRQCIVDYA
HKVG

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively. Has a clear preference for cytidine over uridine. Strictly specific for ribonucleosides [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=277, Percent_Identity=39.7111913357401, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI1786213, Length=285, Percent_Identity=41.4035087719298, Blast_Score=167, Evalue=8e-43,
Organism=Escherichia coli, GI1786871, Length=319, Percent_Identity=36.6771159874608, Blast_Score=160, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI32565474, Length=193, Percent_Identity=38.860103626943, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17565698, Length=199, Percent_Identity=35.678391959799, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320608, Length=257, Percent_Identity=30.3501945525292, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24641837, Length=205, Percent_Identity=32.6829268292683, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24641839, Length=194, Percent_Identity=29.3814432989691, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022977 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: =3.2.2.8 [H]

Molecular weight: Translated: 35702; Mature: 35702

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKIILDTDPGIDDVMAIMFAEAHPEVELRAITTIFGNVTIDTATHNALYLKEKYQLKA
CCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEEEECCEEEECCCCCEEEEEHHHHHHH
DVVKGADKPLVRPPVGPSVVVHGESGFGDVEVPNEVEAVADPRSAHQYIIDAVKAEPNEI
HHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCEE
TLVAIGPLTNLALALQAEPEIVNLVKEVVIMGGAFGVNDHRGNVTPYAEANIHDDPHAAD
EEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCEE
IVFSASWPVVIIGLDVTEQSFFTGDYLEQLRDDAGELGQFIWDVSRFYLKFYRDKVGLDA
EEEECCCCEEEEEEEECCCHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCE
CHVHDPSAIAYVIKPDLFTLREGPVRVVTEGPAEGMTVQKFDGRKYLHDDWENHTPQRVG
EECCCCCEEEEEECCCEEEECCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCHHEE
VAVKDAELLKLYRQCIVDYAHKVG
EEECCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKKIILDTDPGIDDVMAIMFAEAHPEVELRAITTIFGNVTIDTATHNALYLKEKYQLKA
CCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEEEECCEEEECCCCCEEEEEHHHHHHH
DVVKGADKPLVRPPVGPSVVVHGESGFGDVEVPNEVEAVADPRSAHQYIIDAVKAEPNEI
HHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCEE
TLVAIGPLTNLALALQAEPEIVNLVKEVVIMGGAFGVNDHRGNVTPYAEANIHDDPHAAD
EEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCEE
IVFSASWPVVIIGLDVTEQSFFTGDYLEQLRDDAGELGQFIWDVSRFYLKFYRDKVGLDA
EEEECCCCEEEEEEEECCCHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCE
CHVHDPSAIAYVIKPDLFTLREGPVRVVTEGPAEGMTVQKFDGRKYLHDDWENHTPQRVG
EECCCCCEEEEEECCCEEEECCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCHHEE
VAVKDAELLKLYRQCIVDYAHKVG
EEECCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA