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The map label for this gene is ykoW [H]

Identifier: 157960830

GI number: 157960830

Start: 1219028

End: 1220359

Strand: Direct

Name: ykoW [H]

Synonym: Spea_1002

Alternate gene names: 157960830

Gene position: 1219028-1220359 (Clockwise)

Preceding gene: 157960823

Following gene: 157960831

Centisome position: 23.56

GC content: 39.34

Gene sequence:

>1332_bases
ATGAGCATTCGCTTCAAACTTGGACTGCTTTTGTCTTTACTGTTTTTTGCCGCAATTGGTAATACAGTATTCACATTTAT
ACTCGAAAAATATGGTGAAGAAAAACTTGAGTGGGTGATGCATACCCATGAAGTACTAATCGAGTCGGAACGACTGATTA
GTGCGGTTATCGACGCTGAAACGGGTCAGCGAGGCTATCTTTTAACTCAAGATTCAGCATATCTTGAGCCATACCATATT
GGGGTGTCATTGGTGGATAGCCATCTTCAAGAACTCTTTAAGCTAACTTCAGATAATTTGGCGCAGCAGCAGCGCTTAGA
AAATATAAGTGCCTTATTGAGCAAAAAGCTCGCCGAGCTAGATTTAACGATTAACTTGACCCAAGAAAATACCCCCTCCA
GCATATCTGAAGCGTCTAACATTGTTAAAAATAACAGTGGTAAAAAGTATATGGATGCCATTAGGGGGGAGCTCGGTGCG
TTTAACCATGAGGAACTCGTGCTGCTAGAGCAAAGGAAAGGTGAGTTTAAAGAGAGCCGTGCATATATAACGACATTGGT
TGGTGTTGAGCTTCTGTTTTTTGTTTTTTTGTCGATAATTACCGCAATGTTTGTCAAAAATAAATTCTATCAACCATTAG
GCATGATGATCGAAGGTACGGCTAAAATGGAGCGGGGTGAAAGACAAGAGATTTCAGATATCCTGCCTAAGGATGAGATG
GGATATTTATTGTCGCGTTTCTATCAAATGAGTGAAACTGTATATACAAAAGCAATGGTACTCGATCACGAAGCTAACCA
TGATTACCTAACCGGATTAAAGAATCGTTCAGGCTTAGAGGCTGGAGTTGATTATTCGATACAAACACTGAATCACGGCC
AAAAGTTAGCAATATTTTTTATAGACTTAAATAAGTTCAAAGCGTTGAACGATACCTTAGGCCATGATATTGGAGATGAA
ATTTTAAAGGAATCTGCTGTACGCATTAAAGACTCGGTGAGATCGAATGATGCTGTCTATCGCTTCGGCGGGGATGAATT
TGTTGTTATTTTAAACGACATTAAAGAAGTGAAGCATGCACAAGTGATAGCCGAAAAAATGCTGACAAAATTCAGACCGC
CATTTATCTGCAAGGGAAACTTGATAGACATATCACTGAGTATTGGCATCTCTATTTCGCCTGAAGACTCATCAGAAAGC
CGTGAATTATTAAAGCAGGCTGATATGGCGATGTACGCAGCTAAAAGTGATATGAGTGTCAATTATAAATTCTATGATAG
TTCAATGTTGAACCGAGAAAATAGCAATAGCCGTATTGCTTCAGTATCATGA

Upstream 100 bases:

>100_bases
GTCACCTTTTTGCTCTTGATAATGTAGGTGCTAAGTCGCTCCATTTTATGTGTAAGTCATCCATTATAATAATATAAGTC
GTACATTTTAAGGGGATCTA

Downstream 100 bases:

>100_bases
GTTTAATGGCATTAATGTTTTCACTAGCGTGATATTAACCCCCATATAATGATGGTACTTCCAAAGAGATAAAAATGCAG
ATCCGAGTTGGACAACTACA

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSIRFKLGLLLSLLFFAAIGNTVFTFILEKYGEEKLEWVMHTHEVLIESERLISAVIDAETGQRGYLLTQDSAYLEPYHI
GVSLVDSHLQELFKLTSDNLAQQQRLENISALLSKKLAELDLTINLTQENTPSSISEASNIVKNNSGKKYMDAIRGELGA
FNHEELVLLEQRKGEFKESRAYITTLVGVELLFFVFLSIITAMFVKNKFYQPLGMMIEGTAKMERGERQEISDILPKDEM
GYLLSRFYQMSETVYTKAMVLDHEANHDYLTGLKNRSGLEAGVDYSIQTLNHGQKLAIFFIDLNKFKALNDTLGHDIGDE
ILKESAVRIKDSVRSNDAVYRFGGDEFVVILNDIKEVKHAQVIAEKMLTKFRPPFICKGNLIDISLSIGISISPEDSSES
RELLKQADMAMYAAKSDMSVNYKFYDSSMLNRENSNSRIASVS

Sequences:

>Translated_443_residues
MSIRFKLGLLLSLLFFAAIGNTVFTFILEKYGEEKLEWVMHTHEVLIESERLISAVIDAETGQRGYLLTQDSAYLEPYHI
GVSLVDSHLQELFKLTSDNLAQQQRLENISALLSKKLAELDLTINLTQENTPSSISEASNIVKNNSGKKYMDAIRGELGA
FNHEELVLLEQRKGEFKESRAYITTLVGVELLFFVFLSIITAMFVKNKFYQPLGMMIEGTAKMERGERQEISDILPKDEM
GYLLSRFYQMSETVYTKAMVLDHEANHDYLTGLKNRSGLEAGVDYSIQTLNHGQKLAIFFIDLNKFKALNDTLGHDIGDE
ILKESAVRIKDSVRSNDAVYRFGGDEFVVILNDIKEVKHAQVIAEKMLTKFRPPFICKGNLIDISLSIGISISPEDSSES
RELLKQADMAMYAAKSDMSVNYKFYDSSMLNRENSNSRIASVS
>Mature_442_residues
SIRFKLGLLLSLLFFAAIGNTVFTFILEKYGEEKLEWVMHTHEVLIESERLISAVIDAETGQRGYLLTQDSAYLEPYHIG
VSLVDSHLQELFKLTSDNLAQQQRLENISALLSKKLAELDLTINLTQENTPSSISEASNIVKNNSGKKYMDAIRGELGAF
NHEELVLLEQRKGEFKESRAYITTLVGVELLFFVFLSIITAMFVKNKFYQPLGMMIEGTAKMERGERQEISDILPKDEMG
YLLSRFYQMSETVYTKAMVLDHEANHDYLTGLKNRSGLEAGVDYSIQTLNHGQKLAIFFIDLNKFKALNDTLGHDIGDEI
LKESAVRIKDSVRSNDAVYRFGGDEFVVILNDIKEVKHAQVIAEKMLTKFRPPFICKGNLIDISLSIGISISPEDSSESR
ELLKQADMAMYAAKSDMSVNYKFYDSSMLNRENSNSRIASVS

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788381, Length=158, Percent_Identity=37.9746835443038, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=33.7209302325581, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI145693134, Length=169, Percent_Identity=35.5029585798817, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1788956, Length=150, Percent_Identity=35.3333333333333, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082007, Length=171, Percent_Identity=35.672514619883, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=30.635838150289, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081881, Length=161, Percent_Identity=34.1614906832298, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787262, Length=170, Percent_Identity=30, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787816, Length=185, Percent_Identity=29.1891891891892, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787802, Length=159, Percent_Identity=28.9308176100629, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081974, Length=109, Percent_Identity=39.4495412844037, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87081977, Length=158, Percent_Identity=34.1772151898734, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=28.8343558282209, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 50091; Mature: 49960

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRFKLGLLLSLLFFAAIGNTVFTFILEKYGEEKLEWVMHTHEVLIESERLISAVIDAE
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TGQRGYLLTQDSAYLEPYHIGVSLVDSHLQELFKLTSDNLAQQQRLENISALLSKKLAEL
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHE
DLTINLTQENTPSSISEASNIVKNNSGKKYMDAIRGELGAFNHEELVLLEQRKGEFKESR
EEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHH
AYITTLVGVELLFFVFLSIITAMFVKNKFYQPLGMMIEGTAKMERGERQEISDILPKDEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEECCHHHCCCCHHHHHHCCCCCHH
GYLLSRFYQMSETVYTKAMVLDHEANHDYLTGLKNRSGLEAGVDYSIQTLNHGQKLAIFF
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHCCCCCCCCCCCCCCEEEECCCCCEEEEEE
IDLNKFKALNDTLGHDIGDEILKESAVRIKDSVRSNDAVYRFGGDEFVVILNDIKEVKHA
EECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEHHHHHHHHH
QVIAEKMLTKFRPPFICKGNLIDISLSIGISISPEDSSESRELLKQADMAMYAAKSDMSV
HHHHHHHHHHCCCCEEEECCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEE
NYKFYDSSMLNRENSNSRIASVS
EEEEECCHHHCCCCCCCEEEECC
>Mature Secondary Structure 
SIRFKLGLLLSLLFFAAIGNTVFTFILEKYGEEKLEWVMHTHEVLIESERLISAVIDAE
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TGQRGYLLTQDSAYLEPYHIGVSLVDSHLQELFKLTSDNLAQQQRLENISALLSKKLAEL
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHE
DLTINLTQENTPSSISEASNIVKNNSGKKYMDAIRGELGAFNHEELVLLEQRKGEFKESR
EEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHH
AYITTLVGVELLFFVFLSIITAMFVKNKFYQPLGMMIEGTAKMERGERQEISDILPKDEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEECCHHHCCCCHHHHHHCCCCCHH
GYLLSRFYQMSETVYTKAMVLDHEANHDYLTGLKNRSGLEAGVDYSIQTLNHGQKLAIFF
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHCCCCCCCCCCCCCCEEEECCCCCEEEEEE
IDLNKFKALNDTLGHDIGDEILKESAVRIKDSVRSNDAVYRFGGDEFVVILNDIKEVKHA
EECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEHHHHHHHHH
QVIAEKMLTKFRPPFICKGNLIDISLSIGISISPEDSSESRELLKQADMAMYAAKSDMSV
HHHHHHHHHHCCCCEEEECCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEE
NYKFYDSSMLNRENSNSRIASVS
EEEEECCHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]