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The map label for this gene is tyrS [H]

Identifier: 157960823

GI number: 157960823

Start: 1213339

End: 1214535

Strand: Direct

Name: tyrS [H]

Synonym: Spea_0995

Alternate gene names: 157960823

Gene position: 1213339-1214535 (Clockwise)

Preceding gene: 157960820

Following gene: 157960830

Centisome position: 23.45

GC content: 46.7

Gene sequence:

>1197_bases
ATGGCTGATTTAGAGCAAGCATTAGCAGAAATAAGACGTGGCACCGACGAGATTTTACTCGAAGCGGATCTACTGGAGAA
GTTGAAAGAAGGTCGCCCGTTAAAAATTAAACTAGGCGCTGACCCAACCGCACCCGATATTCATCTTGGCCACACGGTTA
TTTTGAACAAGATGCGTACTTTCCAAGAGCTAGGCCATGAAGTGATCTTCTTGATTGGTGACTTCACGGGTATGGTCGGT
GACCCTAGTGGTAAGAACAGCACTCGTCCACCTTTGACTCGTGAGCAAGTGCTTGCCAATGCTGAAACTTATAAGCAGCA
GGTTTATAAGATTTTAGATCCTGCTAAGACCCGTATCGAGTTTAACTCTACTTGGTTAGAGCCACTTGGCGCAGCGGGTA
TGATCCGTCTTGCTTCGCAGCAAACCGTTGCTCGTATGATGGAACGTGATGACTTTAAGAAACGTTACGGTTCAGGCCAG
TCAATTGCGATCCATGAATTCATGTATCCATTGCTACAAGGTTACGACTCAGTTGCACTTGAGGCCGATGTTGAGCTAGG
TGGTACCGATCAGAAGTTCAACCTGCTAATGGGTCGTGAACTGCAAAAGTCTGCTGGCCAGAAGCCACAGACTGTGATCA
TGATGCCACTGCTTGAAGGCCTAGACGGCGTTAAGAAGATGTCTAAGTCTGCTCATAACTACATTGGTGTGAGTGAGCCT
GCAGGTGAGATGTTCGGTAAGATCATGTCAATCTCTGATGATTTGATGTGGCGTTACTTCGAGCTACTGTCTTTCCGTCC
TCTGACTGAAGTTGAGCAGTTTAAAGCTGACGTAGATGCGGGTGCTAACCCACGTGATGTGAAAATCGCACTAGCGAAAG
AGATTATCGCACGCTTCCACGACGAAGCTGCTGCTGAAGCTGCACATCAAGAGTTCATCAATCGCTTCCAAAAAGGCGCG
ATTCCAAGCGATATCGAAGAGTTAGAGATTGCTGCTGGTGAAGGCATTGCGATTGCTAACCTGCTAAAAGAAGCGGGTCT
AGTGAACTCAACATCTGATGCGATGCGTATGATTAAGCAAGGTGCGGCTAAGATTGATGGCGATAAGATTGAAGATACTC
GCCTACAATTAACTGCTGGTACTACGGCTGTATTTCAAGTGGGTAAGCGTAAGTTCGCTAAGATCACGCTAGTTTAA

Upstream 100 bases:

>100_bases
TTAGTGATCCCTTTAGTGTACACACTTTCATTTGTGCTTGCTAACAAGTAACATGAGTGCCCATATTTTTTTGATTAGGC
TCCCAGGAGTAACCGCCGAT

Downstream 100 bases:

>100_bases
GCTGATTGACACTGCCCAGCTTAACTCTGCGAGCTTTACTCAGTGAGCTTAAGCACTAGCGACTAAGCCTAAATCGGCTA
GTGGTTATTCGAACAACGGT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MADLEQALAEIRRGTDEILLEADLLEKLKEGRPLKIKLGADPTAPDIHLGHTVILNKMRTFQELGHEVIFLIGDFTGMVG
DPSGKNSTRPPLTREQVLANAETYKQQVYKILDPAKTRIEFNSTWLEPLGAAGMIRLASQQTVARMMERDDFKKRYGSGQ
SIAIHEFMYPLLQGYDSVALEADVELGGTDQKFNLLMGRELQKSAGQKPQTVIMMPLLEGLDGVKKMSKSAHNYIGVSEP
AGEMFGKIMSISDDLMWRYFELLSFRPLTEVEQFKADVDAGANPRDVKIALAKEIIARFHDEAAAEAAHQEFINRFQKGA
IPSDIEELEIAAGEGIAIANLLKEAGLVNSTSDAMRMIKQGAAKIDGDKIEDTRLQLTAGTTAVFQVGKRKFAKITLV

Sequences:

>Translated_398_residues
MADLEQALAEIRRGTDEILLEADLLEKLKEGRPLKIKLGADPTAPDIHLGHTVILNKMRTFQELGHEVIFLIGDFTGMVG
DPSGKNSTRPPLTREQVLANAETYKQQVYKILDPAKTRIEFNSTWLEPLGAAGMIRLASQQTVARMMERDDFKKRYGSGQ
SIAIHEFMYPLLQGYDSVALEADVELGGTDQKFNLLMGRELQKSAGQKPQTVIMMPLLEGLDGVKKMSKSAHNYIGVSEP
AGEMFGKIMSISDDLMWRYFELLSFRPLTEVEQFKADVDAGANPRDVKIALAKEIIARFHDEAAAEAAHQEFINRFQKGA
IPSDIEELEIAAGEGIAIANLLKEAGLVNSTSDAMRMIKQGAAKIDGDKIEDTRLQLTAGTTAVFQVGKRKFAKITLV
>Mature_397_residues
ADLEQALAEIRRGTDEILLEADLLEKLKEGRPLKIKLGADPTAPDIHLGHTVILNKMRTFQELGHEVIFLIGDFTGMVGD
PSGKNSTRPPLTREQVLANAETYKQQVYKILDPAKTRIEFNSTWLEPLGAAGMIRLASQQTVARMMERDDFKKRYGSGQS
IAIHEFMYPLLQGYDSVALEADVELGGTDQKFNLLMGRELQKSAGQKPQTVIMMPLLEGLDGVKKMSKSAHNYIGVSEPA
GEMFGKIMSISDDLMWRYFELLSFRPLTEVEQFKADVDAGANPRDVKIALAKEIIARFHDEAAAEAAHQEFINRFQKGAI
PSDIEELEIAAGEGIAIANLLKEAGLVNSTSDAMRMIKQGAAKIDGDKIEDTRLQLTAGTTAVFQVGKRKFAKITLV

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=422, Percent_Identity=24.4075829383886, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1787925, Length=400, Percent_Identity=26.5, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25145802, Length=423, Percent_Identity=26.4775413711584, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325160, Length=304, Percent_Identity=25, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI20130383, Length=402, Percent_Identity=24.6268656716418, Blast_Score=118, Evalue=9e-27,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 43977; Mature: 43846

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLEQALAEIRRGTDEILLEADLLEKLKEGRPLKIKLGADPTAPDIHLGHTVILNKMRT
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
FQELGHEVIFLIGDFTGMVGDPSGKNSTRPPLTREQVLANAETYKQQVYKILDPAKTRIE
HHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEE
FNSTWLEPLGAAGMIRLASQQTVARMMERDDFKKRYGSGQSIAIHEFMYPLLQGYDSVAL
ECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCEEE
EADVELGGTDQKFNLLMGRELQKSAGQKPQTVIMMPLLEGLDGVKKMSKSAHNYIGVSEP
EECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCC
AGEMFGKIMSISDDLMWRYFELLSFRPLTEVEQFKADVDAGANPRDVKIALAKEIIARFH
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
DEAAAEAAHQEFINRFQKGAIPSDIEELEIAAGEGIAIANLLKEAGLVNSTSDAMRMIKQ
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
GAAKIDGDKIEDTRLQLTAGTTAVFQVGKRKFAKITLV
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
ADLEQALAEIRRGTDEILLEADLLEKLKEGRPLKIKLGADPTAPDIHLGHTVILNKMRT
CCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
FQELGHEVIFLIGDFTGMVGDPSGKNSTRPPLTREQVLANAETYKQQVYKILDPAKTRIE
HHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEE
FNSTWLEPLGAAGMIRLASQQTVARMMERDDFKKRYGSGQSIAIHEFMYPLLQGYDSVAL
ECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCEEE
EADVELGGTDQKFNLLMGRELQKSAGQKPQTVIMMPLLEGLDGVKKMSKSAHNYIGVSEP
EECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCC
AGEMFGKIMSISDDLMWRYFELLSFRPLTEVEQFKADVDAGANPRDVKIALAKEIIARFH
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
DEAAAEAAHQEFINRFQKGAIPSDIEELEIAAGEGIAIANLLKEAGLVNSTSDAMRMIKQ
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
GAAKIDGDKIEDTRLQLTAGTTAVFQVGKRKFAKITLV
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]