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The map label for this gene is dnaG [H]

Identifier: 157960787

GI number: 157960787

Start: 1171207

End: 1172937

Strand: Reverse

Name: dnaG [H]

Synonym: Spea_0959

Alternate gene names: 157960787

Gene position: 1172937-1171207 (Counterclockwise)

Preceding gene: 157960788

Following gene: 157960786

Centisome position: 22.67

GC content: 44.95

Gene sequence:

>1731_bases
ATGGCGATACCTCGTGATTTTATTAATGAGCTAGTAGCTCGCACTGATATTGTCGATTTAATCGACCCCAAAGTGCCCCT
TAAAAAGGCGGGTAAAAACCACTCTGCCTGTTGTCCTTTTCATAGCGAAAAATCTCCCTCTTTCACCGTTAGCAGAGATA
AACAGTTCTATCATTGTTTTGGCTGCGGCGCGCACGGCAACGTCATCGATTTTGTCATGGAATATGACCGACTCGACTTT
GTTGATGCCATTGAAGAACTCGCCGGACAACTCGGATTAGAAGTCCCTAGGGAACAAGGCACAGGTAAACCACGCGACCA
AGAACTAAGCCGTGATTTATATCAGTTAATGGAAGAAGCCAGTAACTTCTTTCAAAATCAGCTAAGACAAAACACAGACA
AACAAAAAGTCCTTGATTACCTTGATCATCGAGGCCTTACAAAAGAAGTGGTTGAACACTTCAATATCGGTTTTGCTCCC
GATGGTTGGGATGGCTTACTCAGCCGTTACCGCCAAAATCAAGATTCGCAAGACAAGCTATTAACCGCAGGTATGGTCAT
CGAGAATGACAGCGGTAAGCGTTACGACCGATTCCGTGACCGATTGATGTTCCCTATTAGAGATAGGCGCGGGCGAGTCA
TCGGCTTCGGCGGCCGAGTTTTAGGAGACGGTACCCCAAAGTACTTGAATTCTCCAGAAACGCCCATATTTCATAAGGGT
AATGAGCTCTACGGCCTCTATGAATTAAAGCAGCGGCACCGTGATCCACAAAGAGTCTTGATTGTTGAAGGCTATATGGA
CGTTGTAGCTTTGGCACAATTTGATGTTGATTATGCGGTCGCCTCCTTAGGCACCTCTACAACAGCGGATCAATTTCAGT
TATTACTTCGCAGTGCAAAAGAAGTCATCTGCTGCTACGACGGCGATAAAGCCGGTAAAGAAGCCGCATGGCGAGCACTA
GAAACGGCCCTGCCGTTATTAAAGCCTGGTAATCAAGTTCGATTCATGTTCCTGCCTGAGGGAGAAGATCCCGATACGCT
GGTAAGACAGATTGGCAAAGAGCAGTTTGAAGCCCAAATTGACCAGGCAATGGCACTACCCGAGTTTTTGTTCGAAAATC
TGGCAAAAACCTACGCCAACGAAGATAAGAGTGTTTTGGCAAAAAATGCCATTAAACTTATAGAGAAAGTGCAGGACACT
GTATTACAGAGCCTGTTACTCGAAGATTTAGCACATAAGCTAAGAATGAATGGTGCAGACGAACTTAAGCGAAAATTTAA
TTTTTCAGCTAAAGCGGCTGAAAAGCCTAGTCAGCACAAAGCGTTGAAAGGGCGCGGTACCCCATTGCGATTAGCCATTG
CATTGTTAGTACAGCACCCACGCTTAGGTGAAAAACTACCTGAGCAACCAGCGCTAAAACATATACAGATGGCCGGCATT
GACTTGCTGACCCTTGTATTAGATTTAACTCGCGGACAAGTGATAAGCAGCGCACAACTACTTGAACAGTTCAGGGGCGA
TAACCAATTCAGCACCCTAAAAAAACTGACCCAATGGGATCATCAAGTGGCGGATGAAAACTTGCTTCAAAAGTTAAAAG
AAACACTCGTGTGGTTAAACAACCAGTATCTTGAGCAACGATACCAAGAGCTGAGTATGAAGCAGAACCTCACGAAAGAA
GAGCGGTTACAGCTTAGTAAGCTAATTTCAGTGATTAAAAACAAGGCTTAG

Upstream 100 bases:

>100_bases
CGGCTTGTTTGTTTTCGAAGTTCCCAAATTTGACTTCACGAAACGTTTGATTTCACGTAACTTTTGATCTCACGTAATAA
GGCGGCATTTTACAACTCTA

Downstream 100 bases:

>100_bases
TTAAATTTCAGCCTCAAAAGTAAACACTTGGACTAGCACAGAGAAGCGCCGAAGGCTATAATTGACGACTTGCGTGGCTG
TTTTTTGCCCCGTCGTTTTA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MAIPRDFINELVARTDIVDLIDPKVPLKKAGKNHSACCPFHSEKSPSFTVSRDKQFYHCFGCGAHGNVIDFVMEYDRLDF
VDAIEELAGQLGLEVPREQGTGKPRDQELSRDLYQLMEEASNFFQNQLRQNTDKQKVLDYLDHRGLTKEVVEHFNIGFAP
DGWDGLLSRYRQNQDSQDKLLTAGMVIENDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
NELYGLYELKQRHRDPQRVLIVEGYMDVVALAQFDVDYAVASLGTSTTADQFQLLLRSAKEVICCYDGDKAGKEAAWRAL
ETALPLLKPGNQVRFMFLPEGEDPDTLVRQIGKEQFEAQIDQAMALPEFLFENLAKTYANEDKSVLAKNAIKLIEKVQDT
VLQSLLLEDLAHKLRMNGADELKRKFNFSAKAAEKPSQHKALKGRGTPLRLAIALLVQHPRLGEKLPEQPALKHIQMAGI
DLLTLVLDLTRGQVISSAQLLEQFRGDNQFSTLKKLTQWDHQVADENLLQKLKETLVWLNNQYLEQRYQELSMKQNLTKE
ERLQLSKLISVIKNKA

Sequences:

>Translated_576_residues
MAIPRDFINELVARTDIVDLIDPKVPLKKAGKNHSACCPFHSEKSPSFTVSRDKQFYHCFGCGAHGNVIDFVMEYDRLDF
VDAIEELAGQLGLEVPREQGTGKPRDQELSRDLYQLMEEASNFFQNQLRQNTDKQKVLDYLDHRGLTKEVVEHFNIGFAP
DGWDGLLSRYRQNQDSQDKLLTAGMVIENDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
NELYGLYELKQRHRDPQRVLIVEGYMDVVALAQFDVDYAVASLGTSTTADQFQLLLRSAKEVICCYDGDKAGKEAAWRAL
ETALPLLKPGNQVRFMFLPEGEDPDTLVRQIGKEQFEAQIDQAMALPEFLFENLAKTYANEDKSVLAKNAIKLIEKVQDT
VLQSLLLEDLAHKLRMNGADELKRKFNFSAKAAEKPSQHKALKGRGTPLRLAIALLVQHPRLGEKLPEQPALKHIQMAGI
DLLTLVLDLTRGQVISSAQLLEQFRGDNQFSTLKKLTQWDHQVADENLLQKLKETLVWLNNQYLEQRYQELSMKQNLTKE
ERLQLSKLISVIKNKA
>Mature_575_residues
AIPRDFINELVARTDIVDLIDPKVPLKKAGKNHSACCPFHSEKSPSFTVSRDKQFYHCFGCGAHGNVIDFVMEYDRLDFV
DAIEELAGQLGLEVPREQGTGKPRDQELSRDLYQLMEEASNFFQNQLRQNTDKQKVLDYLDHRGLTKEVVEHFNIGFAPD
GWDGLLSRYRQNQDSQDKLLTAGMVIENDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKGN
ELYGLYELKQRHRDPQRVLIVEGYMDVVALAQFDVDYAVASLGTSTTADQFQLLLRSAKEVICCYDGDKAGKEAAWRALE
TALPLLKPGNQVRFMFLPEGEDPDTLVRQIGKEQFEAQIDQAMALPEFLFENLAKTYANEDKSVLAKNAIKLIEKVQDTV
LQSLLLEDLAHKLRMNGADELKRKFNFSAKAAEKPSQHKALKGRGTPLRLAIALLVQHPRLGEKLPEQPALKHIQMAGID
LLTLVLDLTRGQVISSAQLLEQFRGDNQFSTLKKLTQWDHQVADENLLQKLKETLVWLNNQYLEQRYQELSMKQNLTKEE
RLQLSKLISVIKNKA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=575, Percent_Identity=51.8260869565217, Blast_Score=578, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65656; Mature: 65525

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPRDFINELVARTDIVDLIDPKVPLKKAGKNHSACCPFHSEKSPSFTVSRDKQFYHCF
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEEEE
GCGAHGNVIDFVMEYDRLDFVDAIEELAGQLGLEVPREQGTGKPRDQELSRDLYQLMEEA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHH
SNFFQNQLRQNTDKQKVLDYLDHRGLTKEVVEHFNIGFAPDGWDGLLSRYRQNQDSQDKL
HHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHH
LTAGMVIENDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
HHHCEEEECCCCCHHHHHHHHCCCCCCCCCCCEEEECCEEECCCCCHHCCCCCCCCEECC
NELYGLYELKQRHRDPQRVLIVEGYMDVVALAQFDVDYAVASLGTSTTADQFQLLLRSAK
CCCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EVICCYDGDKAGKEAAWRALETALPLLKPGNQVRFMFLPEGEDPDTLVRQIGKEQFEAQI
CEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
DQAMALPEFLFENLAKTYANEDKSVLAKNAIKLIEKVQDTVLQSLLLEDLAHKLRMNGAD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ELKRKFNFSAKAAEKPSQHKALKGRGTPLRLAIALLVQHPRLGEKLPEQPALKHIQMAGI
HHHHHHCCCHHHCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
DLLTLVLDLTRGQVISSAQLLEQFRGDNQFSTLKKLTQWDHQVADENLLQKLKETLVWLN
HHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQYLEQRYQELSMKQNLTKEERLQLSKLISVIKNKA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIPRDFINELVARTDIVDLIDPKVPLKKAGKNHSACCPFHSEKSPSFTVSRDKQFYHCF
CCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCEEEECCCCEEEEE
GCGAHGNVIDFVMEYDRLDFVDAIEELAGQLGLEVPREQGTGKPRDQELSRDLYQLMEEA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHH
SNFFQNQLRQNTDKQKVLDYLDHRGLTKEVVEHFNIGFAPDGWDGLLSRYRQNQDSQDKL
HHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHH
LTAGMVIENDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
HHHCEEEECCCCCHHHHHHHHCCCCCCCCCCCEEEECCEEECCCCCHHCCCCCCCCEECC
NELYGLYELKQRHRDPQRVLIVEGYMDVVALAQFDVDYAVASLGTSTTADQFQLLLRSAK
CCCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EVICCYDGDKAGKEAAWRALETALPLLKPGNQVRFMFLPEGEDPDTLVRQIGKEQFEAQI
CEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
DQAMALPEFLFENLAKTYANEDKSVLAKNAIKLIEKVQDTVLQSLLLEDLAHKLRMNGAD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ELKRKFNFSAKAAEKPSQHKALKGRGTPLRLAIALLVQHPRLGEKLPEQPALKHIQMAGI
HHHHHHCCCHHHCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
DLLTLVLDLTRGQVISSAQLLEQFRGDNQFSTLKKLTQWDHQVADENLLQKLKETLVWLN
HHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQYLEQRYQELSMKQNLTKEERLQLSKLISVIKNKA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]