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The map label for this gene is puuB [H]

Identifier: 157960770

GI number: 157960770

Start: 1149649

End: 1150956

Strand: Reverse

Name: puuB [H]

Synonym: Spea_0942

Alternate gene names: 157960770

Gene position: 1150956-1149649 (Counterclockwise)

Preceding gene: 157960781

Following gene: 157960764

Centisome position: 22.24

GC content: 48.78

Gene sequence:

>1308_bases
ATGTCAACTAGAAGCCCGGTGCATAGCGACCAATATCCCGCATCATATTACTTTGCAACAGCGAAAGAATTACATCAATC
ACCACGCTTAGAAGAGGTGCTTGATGTAGATGTGTGTGTGGTGGGCGGTGGATTTAGCGGTATTAATACCGCTATCGAAT
TAGCACAAAAAGGCTTCAGTGTGGCGTTACTTGAGGCTAAGCGGATCGGCTGGGGCGCTTCTGGGCGTAACGGTGGAGAA
CTTATCCGTGGAATTGGTCATGACGTTAGCCAGTTTAGCAATATCATTGGCAGCGAAGGCGTTAAGGCCATCGAGCAGAT
GGGCTTCGAGGCAGTAGATATCGTTAGGCAACGCGTAGCCGAGCACAATATCGATTGTAACTTGCAGATGGGTTACTGCG
ACTTAGCGATTCGACCCCGCCATATGACGGAGCTTGAAGAAGATTTTGAACACTTGAGCAAGATAGGCTACGGCCACAAC
ATGACCTTACTCGACAAGTCTCGCATCGGTGAGGTTATAGGCTCCGATTTTTATCAAGGCGCCTTAGTGGATATGGCCAG
TGGTCACCTGCATCCGCTTAACTTAGCCCTAGGCGAAGCCCGAGTTGCCCGCAGCTTAGGGGTTAAGATGTTTGAATACA
GCGCCGCAACAAAAATCATTAAGGGCGATAAGCCTAAGGTGATTACTGAGCATGGCGAAGTCAATTGCCAATACTTAGTC
CTTGCTGGCAATGCCTACTTAGGCGTCGATTTAGAACAGAACATTGGCAGCAAAGTATTACCTGCTGGCACCTACATCTT
GGCCACCGAGCCGCTAACGCAGGAGCAATGTGATGCCATCATTCCTAAAAATATGGCCTTTGCCGACTTAAGGGTTGATC
TTGATTACTACCATCTATCTGAAGATAACCGCCTGCTATTTGGCGGTCTGTGTACCTATTCTGGTAAAGATCCAAAAGAT
ATCGAAGCCGCTCTAAGACCCAATCTTGAAAAAGTATTCCCGCAGCTAAAAGGCGTGCGTATCGACTATGAATGGGGCGG
CATGATGGGCATCGGCGCTAACCGATTACCGCAAATCGGTCGTCTACCCGATGCCAAGAATATCTTTTATGCCCAAGCGT
ATGCGGGTCACGGCGTTAATGCCACCCATATGGCAGCAAAGCTAATCGCCGAGGCAATTACCACTCAAGCCGAACGTTTC
GATGTGTTTGCCAAAATCCCCCATATGACCTTTCCCGGCGGCCCGCACCTGCGCTCGCCACTATTAGCAATGGGCATGCT
GTACCATAGATTCAAAGATATTTTTTAA

Upstream 100 bases:

>100_bases
GTGTTAAAAATAACGAACATATGTAAATAATTTATTTATATAACATAGAATATACCGTCAGCATTCCAACTTACAATAAA
AACATATACTTGGAGAGAAG

Downstream 100 bases:

>100_bases
CCGTGTTTTAAAAGCGATCAACCACGAAGCGTTCACTCTGTTCACTACACTGAGGACACGAAGAAAAGAAAATATCAGGT
TAGCTTTTCTTCGTATTCTT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSTRSPVHSDQYPASYYFATAKELHQSPRLEEVLDVDVCVVGGGFSGINTAIELAQKGFSVALLEAKRIGWGASGRNGGE
LIRGIGHDVSQFSNIIGSEGVKAIEQMGFEAVDIVRQRVAEHNIDCNLQMGYCDLAIRPRHMTELEEDFEHLSKIGYGHN
MTLLDKSRIGEVIGSDFYQGALVDMASGHLHPLNLALGEARVARSLGVKMFEYSAATKIIKGDKPKVITEHGEVNCQYLV
LAGNAYLGVDLEQNIGSKVLPAGTYILATEPLTQEQCDAIIPKNMAFADLRVDLDYYHLSEDNRLLFGGLCTYSGKDPKD
IEAALRPNLEKVFPQLKGVRIDYEWGGMMGIGANRLPQIGRLPDAKNIFYAQAYAGHGVNATHMAAKLIAEAITTQAERF
DVFAKIPHMTFPGGPHLRSPLLAMGMLYHRFKDIF

Sequences:

>Translated_435_residues
MSTRSPVHSDQYPASYYFATAKELHQSPRLEEVLDVDVCVVGGGFSGINTAIELAQKGFSVALLEAKRIGWGASGRNGGE
LIRGIGHDVSQFSNIIGSEGVKAIEQMGFEAVDIVRQRVAEHNIDCNLQMGYCDLAIRPRHMTELEEDFEHLSKIGYGHN
MTLLDKSRIGEVIGSDFYQGALVDMASGHLHPLNLALGEARVARSLGVKMFEYSAATKIIKGDKPKVITEHGEVNCQYLV
LAGNAYLGVDLEQNIGSKVLPAGTYILATEPLTQEQCDAIIPKNMAFADLRVDLDYYHLSEDNRLLFGGLCTYSGKDPKD
IEAALRPNLEKVFPQLKGVRIDYEWGGMMGIGANRLPQIGRLPDAKNIFYAQAYAGHGVNATHMAAKLIAEAITTQAERF
DVFAKIPHMTFPGGPHLRSPLLAMGMLYHRFKDIF
>Mature_434_residues
STRSPVHSDQYPASYYFATAKELHQSPRLEEVLDVDVCVVGGGFSGINTAIELAQKGFSVALLEAKRIGWGASGRNGGEL
IRGIGHDVSQFSNIIGSEGVKAIEQMGFEAVDIVRQRVAEHNIDCNLQMGYCDLAIRPRHMTELEEDFEHLSKIGYGHNM
TLLDKSRIGEVIGSDFYQGALVDMASGHLHPLNLALGEARVARSLGVKMFEYSAATKIIKGDKPKVITEHGEVNCQYLVL
AGNAYLGVDLEQNIGSKVLPAGTYILATEPLTQEQCDAIIPKNMAFADLRVDLDYYHLSEDNRLLFGGLCTYSGKDPKDI
EAALRPNLEKVFPQLKGVRIDYEWGGMMGIGANRLPQIGRLPDAKNIFYAQAYAGHGVNATHMAAKLIAEAITTQAERFD
VFAKIPHMTFPGGPHLRSPLLAMGMLYHRFKDIF

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=423, Percent_Identity=43.2624113475177, Blast_Score=370, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47784; Mature: 47653

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTRSPVHSDQYPASYYFATAKELHQSPRLEEVLDVDVCVVGGGFSGINTAIELAQKGFS
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCEEEEECCCCHHHHHHHHHHHCCCE
VALLEAKRIGWGASGRNGGELIRGIGHDVSQFSNIIGSEGVKAIEQMGFEAVDIVRQRVA
EEEEEHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHH
EHNIDCNLQMGYCDLAIRPRHMTELEEDFEHLSKIGYGHNMTLLDKSRIGEVIGSDFYQG
HCCCCEEEEECEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCHHHCC
ALVDMASGHLHPLNLALGEARVARSLGVKMFEYSAATKIIKGDKPKVITEHGEVNCQYLV
HHHHHCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEE
LAGNAYLGVDLEQNIGSKVLPAGTYILATEPLTQEQCDAIIPKNMAFADLRVDLDYYHLS
EECCEEEEEEHHHCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCEEEEEEEEEEEEEEC
EDNRLLFGGLCTYSGKDPKDIEAALRPNLEKVFPQLKGVRIDYEWGGMMGIGANRLPQIG
CCCEEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHCCEEEEEECCCEEECCCCCCCCCC
RLPDAKNIFYAQAYAGHGVNATHMAAKLIAEAITTQAERFDVFAKIPHMTFPGGPHLRSP
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
LLAMGMLYHRFKDIF
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
STRSPVHSDQYPASYYFATAKELHQSPRLEEVLDVDVCVVGGGFSGINTAIELAQKGFS
CCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCEEEEECCCCHHHHHHHHHHHCCCE
VALLEAKRIGWGASGRNGGELIRGIGHDVSQFSNIIGSEGVKAIEQMGFEAVDIVRQRVA
EEEEEHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHH
EHNIDCNLQMGYCDLAIRPRHMTELEEDFEHLSKIGYGHNMTLLDKSRIGEVIGSDFYQG
HCCCCEEEEECEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCHHHCC
ALVDMASGHLHPLNLALGEARVARSLGVKMFEYSAATKIIKGDKPKVITEHGEVNCQYLV
HHHHHCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEE
LAGNAYLGVDLEQNIGSKVLPAGTYILATEPLTQEQCDAIIPKNMAFADLRVDLDYYHLS
EECCEEEEEEHHHCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCEEEEEEEEEEEEEEC
EDNRLLFGGLCTYSGKDPKDIEAALRPNLEKVFPQLKGVRIDYEWGGMMGIGANRLPQIG
CCCEEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHCCEEEEEECCCEEECCCCCCCCCC
RLPDAKNIFYAQAYAGHGVNATHMAAKLIAEAITTQAERFDVFAKIPHMTFPGGPHLRSP
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
LLAMGMLYHRFKDIF
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]