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The map label for this gene is folK [H]

Identifier: 157960523

GI number: 157960523

Start: 855493

End: 855987

Strand: Reverse

Name: folK [H]

Synonym: Spea_0695

Alternate gene names: 157960523

Gene position: 855987-855493 (Counterclockwise)

Preceding gene: 157960524

Following gene: 157960522

Centisome position: 16.54

GC content: 50.91

Gene sequence:

>495_bases
ATGGCGCAAGTCTATGTCGCTTTGGGCGCTAACTTAGCGGACCCAATTAGCCAGCTCAATAATGCCTGTGAGGCATTGTT
GAGCTTGGCGCATGCAGATTCCTTCAGCGTTTCTTCCTATTATCGCTCAGTCCCTATGGGTGATGTCGAGCAGCCAGACT
ACGTTAATGCAGTCGCAGGCTTTCAGACCATGCTCGCACCCATTGCGCTACTCGATGCCTTGCAGAGTATTGAAAACCAG
CAAGGCCGCGTGAGAGAACTACGCTGGGGACCGAGAACGCTCGATTTAGACCTATTGCTTTATGACAATCAGCTTATCGA
TACCCCTCGCTTAACCGTGCCCCATTATGGCATGCGTGAGCGCAGTTTCGTATTGGTACCGCTTGCCGATATTGCACCTG
AGCTTATCTTGCCGGACAATACTCCACTTTCAAGTTTACTCAACGAGTCCTTGAGAGCCGAGCTTGTGTTAGCTCAAGCT
CCGACGGCTGAATAA

Upstream 100 bases:

>100_bases
TGATTTCTCGCTCTACCAATAAAGGTAGTCGTAACCGCAGCTCTAACCAACGTCGCCGCCGTAAGCCAAGCGCTAAATCG
GCACCTAAATCTGCTGAATA

Downstream 100 bases:

>100_bases
GTGACATTCAGCCGTTGCGATTTTCTGTTAGTATATTTCGCATCTGTTATATAAGGGTTTAACACCGAGATCATTGAAGT
GAGCCCATGTTGGCCTATAA

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MAQVYVALGANLADPISQLNNACEALLSLAHADSFSVSSYYRSVPMGDVEQPDYVNAVAGFQTMLAPIALLDALQSIENQ
QGRVRELRWGPRTLDLDLLLYDNQLIDTPRLTVPHYGMRERSFVLVPLADIAPELILPDNTPLSSLLNESLRAELVLAQA
PTAE

Sequences:

>Translated_164_residues
MAQVYVALGANLADPISQLNNACEALLSLAHADSFSVSSYYRSVPMGDVEQPDYVNAVAGFQTMLAPIALLDALQSIENQ
QGRVRELRWGPRTLDLDLLLYDNQLIDTPRLTVPHYGMRERSFVLVPLADIAPELILPDNTPLSSLLNESLRAELVLAQA
PTAE
>Mature_163_residues
AQVYVALGANLADPISQLNNACEALLSLAHADSFSVSSYYRSVPMGDVEQPDYVNAVAGFQTMLAPIALLDALQSIENQQ
GRVRELRWGPRTLDLDLLLYDNQLIDTPRLTVPHYGMRERSFVLVPLADIAPELILPDNTPLSSLLNESLRAELVLAQAP
TAE

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=149, Percent_Identity=51.6778523489933, Blast_Score=160, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324073, Length=143, Percent_Identity=31.4685314685315, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 17974; Mature: 17843

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQVYVALGANLADPISQLNNACEALLSLAHADSFSVSSYYRSVPMGDVEQPDYVNAVAG
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCHHHHHHH
FQTMLAPIALLDALQSIENQQGRVRELRWGPRTLDLDLLLYDNQLIDTPRLTVPHYGMRE
HHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCC
RSFVLVPLADIAPELILPDNTPLSSLLNESLRAELVLAQAPTAE
CCEEEEEHHHHCCCEECCCCCCHHHHHHHHHHHEEEEECCCCCC
>Mature Secondary Structure 
AQVYVALGANLADPISQLNNACEALLSLAHADSFSVSSYYRSVPMGDVEQPDYVNAVAG
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCHHHHHHH
FQTMLAPIALLDALQSIENQQGRVRELRWGPRTLDLDLLLYDNQLIDTPRLTVPHYGMRE
HHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCC
RSFVLVPLADIAPELILPDNTPLSSLLNESLRAELVLAQAPTAE
CCEEEEEHHHHCCCEECCCCCCHHHHHHHHHHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10080886 [H]