Click here to switch to the map view.

The map label for this gene is rsmE [H]

Identifier: 157960429

GI number: 157960429

Start: 738034

End: 738765

Strand: Reverse

Name: rsmE [H]

Synonym: Spea_0600

Alternate gene names: 157960429

Gene position: 738765-738034 (Counterclockwise)

Preceding gene: 157960431

Following gene: 157960428

Centisome position: 14.28

GC content: 48.36

Gene sequence:

>732_bases
ATGAGAGTGCCAAGAATCTATCAACAATCAACATTAGTTGTCGGCCAATCGGTCGCATTAGATGATGAAGCGGCAGGTCA
TGTTGGCCGCGTATTAAGAATGTCTTCAGGTGAGCAGGTTAGCTTGTTCAATGGTGACGGCAATGACTATCTTGCTGAGA
TTATTAGCGCTAGTAAGAAAAACGTCGAAGTTAAGGTCCTCTCATGCGAGGCAAATGATAGCGAATCGCCACTTAACTTA
CATTTAGGCCAAGTGATCTCTCGTGGCGATAGAATGGATTTCACCATTCAAAAGTCGGTTGAGCTGGGCGTGAACACCAT
CACGCCACTGTTTTCAGAGCGCTGTGGGGTCAAATTAACTGGCGAGCGTTTAGATAAAAAAATTCAACAATGGCAAAAGA
TTGTCATTAGCGCCTGCGAGCAATCTGGCCGTAGTGTGGTGCCGGTTATCCGCCCTGCGATGCAACTTAGCGATTGGTGT
GCTGAAGATTCTGACGCGGTGAAATTGAACCTGCATCCGCGAGCCTCACACGGTGTTAACGGGCTGACACTGCCTAATAA
CCGTGTCAGATTGGTTATTGGGCCAGAAGGTGGTTTATCAGCGCCAGAAATCGCCATGACCGAAGAGCACCAATTTACCG
ACGTACTGCTTGGCCCACGAGTACTGCGCACAGAAACCGCAGCGCTCACCACCATTAGCGCTTTGCAGCTAAAATTTGGT
GATATCGGCTAA

Upstream 100 bases:

>100_bases
CGTGACCAAGTGATTGCTATGCTTCAAAAGCAACGCCAACAAGTTGGTATAACAGCTGCTAAATTACTCATCAACATATG
CAAAAAAGGCTAATTACATC

Downstream 100 bases:

>100_bases
ACGCTTTACATGGCAAACTCTGCCAAGTAGAAGATTTATTAGTAGCACGCTAGAAAACGGGGGCTTATGCCCCCATATTT
ATCGTTGAAACGAATATAAG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRVPRIYQQSTLVVGQSVALDDEAAGHVGRVLRMSSGEQVSLFNGDGNDYLAEIISASKKNVEVKVLSCEANDSESPLNL
HLGQVISRGDRMDFTIQKSVELGVNTITPLFSERCGVKLTGERLDKKIQQWQKIVISACEQSGRSVVPVIRPAMQLSDWC
AEDSDAVKLNLHPRASHGVNGLTLPNNRVRLVIGPEGGLSAPEIAMTEEHQFTDVLLGPRVLRTETAALTTISALQLKFG
DIG

Sequences:

>Translated_243_residues
MRVPRIYQQSTLVVGQSVALDDEAAGHVGRVLRMSSGEQVSLFNGDGNDYLAEIISASKKNVEVKVLSCEANDSESPLNL
HLGQVISRGDRMDFTIQKSVELGVNTITPLFSERCGVKLTGERLDKKIQQWQKIVISACEQSGRSVVPVIRPAMQLSDWC
AEDSDAVKLNLHPRASHGVNGLTLPNNRVRLVIGPEGGLSAPEIAMTEEHQFTDVLLGPRVLRTETAALTTISALQLKFG
DIG
>Mature_243_residues
MRVPRIYQQSTLVVGQSVALDDEAAGHVGRVLRMSSGEQVSLFNGDGNDYLAEIISASKKNVEVKVLSCEANDSESPLNL
HLGQVISRGDRMDFTIQKSVELGVNTITPLFSERCGVKLTGERLDKKIQQWQKIVISACEQSGRSVVPVIRPAMQLSDWC
AEDSDAVKLNLHPRASHGVNGLTLPNNRVRLVIGPEGGLSAPEIAMTEEHQFTDVLLGPRVLRTETAALTTISALQLKFG
DIG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=65.843621399177, Blast_Score=337, Evalue=5e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26397; Mature: 26397

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVPRIYQQSTLVVGQSVALDDEAAGHVGRVLRMSSGEQVSLFNGDGNDYLAEIISASKK
CCCCCCCCCCEEEEECEEECCCHHHHHHHHHEECCCCCEEEEEECCCHHHHHHHHHCCCC
NVEVKVLSCEANDSESPLNLHLGQVISRGDRMDFTIQKSVELGVNTITPLFSERCGVKLT
CEEEEEEEECCCCCCCCCEEHHHHHHCCCCCCEEEEECHHHCCCHHHCHHHHHCCCCEEE
GERLDKKIQQWQKIVISACEQSGRSVVPVIRPAMQLSDWCAEDSDAVKLNLHPRASHGVN
HHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
GLTLPNNRVRLVIGPEGGLSAPEIAMTEEHQFTDVLLGPRVLRTETAALTTISALQLKFG
CEECCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCCCHHHHHHHHHHHHHEEEEECC
DIG
CCC
>Mature Secondary Structure
MRVPRIYQQSTLVVGQSVALDDEAAGHVGRVLRMSSGEQVSLFNGDGNDYLAEIISASKK
CCCCCCCCCCEEEEECEEECCCHHHHHHHHHEECCCCCEEEEEECCCHHHHHHHHHCCCC
NVEVKVLSCEANDSESPLNLHLGQVISRGDRMDFTIQKSVELGVNTITPLFSERCGVKLT
CEEEEEEEECCCCCCCCCEEHHHHHHCCCCCCEEEEECHHHCCCHHHCHHHHHCCCCEEE
GERLDKKIQQWQKIVISACEQSGRSVVPVIRPAMQLSDWCAEDSDAVKLNLHPRASHGVN
HHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
GLTLPNNRVRLVIGPEGGLSAPEIAMTEEHQFTDVLLGPRVLRTETAALTTISALQLKFG
CEECCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCCCHHHHHHHHHHHHHEEEEECC
DIG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]