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The map label for this gene is def [C]

Identifier: 157825792

GI number: 157825792

Start: 654461

End: 654850

Strand: Direct

Name: def [C]

Synonym: A1C_03615

Alternate gene names: 157825792

Gene position: 654461-654850 (Clockwise)

Preceding gene: 157825791

Following gene: 157825793

Centisome position: 53.16

GC content: 31.79

Gene sequence:

>390_bases
ATGGTAGGAATATTAAAGCGTATAGCAATAGTTGAGCTACATGAAAATAATAAATCATCGCCTATAGTGTTGATTAATCC
GGAAATAACTTATTTTTCGGAAGCAAAACAAACTTTTATTGAGTGTTCTTTATCATTTCCAGGCATTGAAGCCTCTATTA
CACGCTCAAAAGTAATCAAGGTAAAATATCTCGATTATGACGGTAATAAACAAGAACTAGACGCAGAAGGCTTTTTAGCG
ACTGTAATTCAACATAAAATAGATTATTTAAACGGTAAAACTTTCTTAGATTATTTATCAAAACTTAAGCGTGATATGCT
CCTTAAAAAGATGCTCAAGCATATAAAACTCCATCCTCCACATATTCACGGTAACACTTGTAGACATTAA

Upstream 100 bases:

>100_bases
CAGAATATATAGACATTGTTGATGATAATATCCGTACTATTTTGTCGATCAAATGCTTCAGACTTTACATATAGAAAGGG
CAGTTGGCCTCAGTGCTAAT

Downstream 100 bases:

>100_bases
TTAATTCTTAAAATAATAAAACTTTCTTGACTATTAATAGAAAAAGTATAATTTATTTACAATTTTATTTTTTATTAATA
GATATGAAGGAAATATTTTA

Product: polypeptide deformylase

Products: Formate; L-Methionine. [C]

Alternate protein names: Polypeptide deformylase-like [H]

Number of amino acids: Translated: 129; Mature: 129

Protein sequence:

>129_residues
MVGILKRIAIVELHENNKSSPIVLINPEITYFSEAKQTFIECSLSFPGIEASITRSKVIKVKYLDYDGNKQELDAEGFLA
TVIQHKIDYLNGKTFLDYLSKLKRDMLLKKMLKHIKLHPPHIHGNTCRH

Sequences:

>Translated_129_residues
MVGILKRIAIVELHENNKSSPIVLINPEITYFSEAKQTFIECSLSFPGIEASITRSKVIKVKYLDYDGNKQELDAEGFLA
TVIQHKIDYLNGKTFLDYLSKLKRDMLLKKMLKHIKLHPPHIHGNTCRH
>Mature_129_residues
MVGILKRIAIVELHENNKSSPIVLINPEITYFSEAKQTFIECSLSFPGIEASITRSKVIKVKYLDYDGNKQELDAEGFLA
TVIQHKIDYLNGKTFLDYLSKLKRDMLLKKMLKHIKLHPPHIHGNTCRH

Specific function: Removes The Formyl Group From The N-Terminal Met Of Newly Synthesized Proteins. Requires At Least A Dipeptide For An Efficient Rate Of Reaction. N-Terminal L-Methionine Is A Prerequisite For Activity But The Enzyme Has Broad Specificity At Other Positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=113, Percent_Identity=41.5929203539823, Blast_Score=81, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: 3.5.1.88 [C]

Molecular weight: Translated: 14833; Mature: 14833

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGILKRIAIVELHENNKSSPIVLINPEITYFSEAKQTFIECSLSFPGIEASITRSKVIK
CCCHHHHEEEEEECCCCCCCCEEEECCCEEEHHHHHHHHHEEECCCCCCCCEECCCEEEE
VKYLDYDGNKQELDAEGFLATVIQHKIDYLNGKTFLDYLSKLKRDMLLKKMLKHIKLHPP
EEEECCCCCHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
HIHGNTCRH
CCCCCCCCC
>Mature Secondary Structure
MVGILKRIAIVELHENNKSSPIVLINPEITYFSEAKQTFIECSLSFPGIEASITRSKVIK
CCCHHHHEEEEEECCCCCCCCEEEECCCEEEHHHHHHHHHEEECCCCCCCCEECCCEEEE
VKYLDYDGNKQELDAEGFLATVIQHKIDYLNGKTFLDYLSKLKRDMLLKKMLKHIKLHPP
EEEECCCCCHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
HIHGNTCRH
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Formyl-L-Methionine; H2O [C]

Specific reaction: N-Formyl-L-Methionine + H2O = Formate + L-Methionine. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: Dithiothreitol; EDTA; o-Phenantroline [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]