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The map label for this gene is dacF [H]

Identifier: 157825663

GI number: 157825663

Start: 528910

End: 529830

Strand: Direct

Name: dacF [H]

Synonym: A1C_02925

Alternate gene names: 157825663

Gene position: 528910-529830 (Clockwise)

Preceding gene: 157825662

Following gene: 157825671

Centisome position: 42.96

GC content: 34.64

Gene sequence:

>921_bases
ATGTTTAGTAGATTTTTATTACCTTATATTTTTATTGTAACATTTTTTTTTGCTCCCATAAGCGAAGCGAAGAAAGCTGT
AAAAAATACCCCTAAACCTCCTGTTCAAACGAGTTTGGTAATTGATGCAAAAAGCGGTAAAGTGCTGCAGGCTCATAATT
CACAAATTCGGGTTTATCCTGCTTCTTTAACTAAGCTAATGACTCTGTACTTAATGTTTGAAGCAATTGAAGACGGTAAG
TTATCTTTAGATCAAAAATTACTTGTCTCACAGAAAGCTTCAAAAATGCCGCCCTGTAAGCTTGGGTTAAAGGCAGGAGA
GACTATAACCGTTAGAGAGGCAATTAACGGATTAATCATAAAATCTGCTAATGATGCTGCCGTAACTGTTGCTGAGGGGA
TGAAAGGTTCTGAGAAAGCTTTTGCTCATTTAATGAATATTAAAGCTAAACAGCTCGGTATGAAAAATACCTATTTTAAA
AATGCTTCAGGCTGGCATGACCCATTGCAGCTGACTACCGCAAGAGATATGGCTAAACTTACTATGGCTTTGAAAAAAGA
TTATCCTAAATATTATCCACTCTTTTCTAAAACCAGTTTTGTATTTCGTGGCAACATAGTAAATGGACATAATAGGGTTA
CTAAAAATTATCAAGGTGCAGAAGGTATGAAAACAGGTTTTCATACACCAGCAGGTTATAATTTAGTAACTACTGCTTCT
AGGAACGGTAAATCATTGATTGCCGTTGTTACAGGAGGCAAAAGTGCCGCATCTCGTGATCAAAAAATGATGTGGCTACT
AGATCAGCATTTTAATATTAAGCCTATAGTCCATAAACCTTTAATCAAACTTGCATCTAATAATAAATCTACTACAAAAG
CTAAATCAAAAATAATAAAGAAAAAGAATATACGTAGTTGA

Upstream 100 bases:

>100_bases
CCACGTCGCTTCGCTCCTCGCGATGACGAAATCTATACTTATTGAGCAATGGTGAAAAATCCCAGTTGATTTAATTTGAA
TAATATTTTATTGACTTAAA

Downstream 100 bases:

>100_bases
TATGTATCATAATTGCCTGCGTGGATCAATTTCACCTCTGTCATCATGTGGCTTGACCACGGGATCCAGTTAAAATACTA
AAATTACTGGTATTTGTTTA

Product: penicillin-binding protein dacF precursor

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MFSRFLLPYIFIVTFFFAPISEAKKAVKNTPKPPVQTSLVIDAKSGKVLQAHNSQIRVYPASLTKLMTLYLMFEAIEDGK
LSLDQKLLVSQKASKMPPCKLGLKAGETITVREAINGLIIKSANDAAVTVAEGMKGSEKAFAHLMNIKAKQLGMKNTYFK
NASGWHDPLQLTTARDMAKLTMALKKDYPKYYPLFSKTSFVFRGNIVNGHNRVTKNYQGAEGMKTGFHTPAGYNLVTTAS
RNGKSLIAVVTGGKSAASRDQKMMWLLDQHFNIKPIVHKPLIKLASNNKSTTKAKSKIIKKKNIRS

Sequences:

>Translated_306_residues
MFSRFLLPYIFIVTFFFAPISEAKKAVKNTPKPPVQTSLVIDAKSGKVLQAHNSQIRVYPASLTKLMTLYLMFEAIEDGK
LSLDQKLLVSQKASKMPPCKLGLKAGETITVREAINGLIIKSANDAAVTVAEGMKGSEKAFAHLMNIKAKQLGMKNTYFK
NASGWHDPLQLTTARDMAKLTMALKKDYPKYYPLFSKTSFVFRGNIVNGHNRVTKNYQGAEGMKTGFHTPAGYNLVTTAS
RNGKSLIAVVTGGKSAASRDQKMMWLLDQHFNIKPIVHKPLIKLASNNKSTTKAKSKIIKKKNIRS
>Mature_306_residues
MFSRFLLPYIFIVTFFFAPISEAKKAVKNTPKPPVQTSLVIDAKSGKVLQAHNSQIRVYPASLTKLMTLYLMFEAIEDGK
LSLDQKLLVSQKASKMPPCKLGLKAGETITVREAINGLIIKSANDAAVTVAEGMKGSEKAFAHLMNIKAKQLGMKNTYFK
NASGWHDPLQLTTARDMAKLTMALKKDYPKYYPLFSKTSFVFRGNIVNGHNRVTKNYQGAEGMKTGFHTPAGYNLVTTAS
RNGKSLIAVVTGGKSAASRDQKMMWLLDQHFNIKPIVHKPLIKLASNNKSTTKAKSKIIKKKNIRS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=254, Percent_Identity=36.6141732283465, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI87082022, Length=272, Percent_Identity=32.7205882352941, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786851, Length=255, Percent_Identity=34.5098039215686, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI87082054, Length=232, Percent_Identity=29.7413793103448, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 33870; Mature: 33870

Theoretical pI: Translated: 10.89; Mature: 10.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSRFLLPYIFIVTFFFAPISEAKKAVKNTPKPPVQTSLVIDAKSGKVLQAHNSQIRVYP
CCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECCCEEEEEH
ASLTKLMTLYLMFEAIEDGKLSLDQKLLVSQKASKMPPCKLGLKAGETITVREAINGLII
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCEEEHHHHHCEEEE
KSANDAAVTVAEGMKGSEKAFAHLMNIKAKQLGMKNTYFKNASGWHDPLQLTTARDMAKL
ECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCEEEHHHHHHHHH
TMALKKDYPKYYPLFSKTSFVFRGNIVNGHNRVTKNYQGAEGMKTGFHTPAGYNLVTTAS
HHHHHHCCCCCCCCCCCCEEEEECCEECCCCHHCCCCCCCCCCCCCCCCCCCCEEEEECC
RNGKSLIAVVTGGKSAASRDQKMMWLLDQHFNIKPIVHKPLIKLASNNKSTTKAKSKIIK
CCCCEEEEEEECCCCHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
KKNIRS
HCCCCC
>Mature Secondary Structure
MFSRFLLPYIFIVTFFFAPISEAKKAVKNTPKPPVQTSLVIDAKSGKVLQAHNSQIRVYP
CCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECCCEEEEEH
ASLTKLMTLYLMFEAIEDGKLSLDQKLLVSQKASKMPPCKLGLKAGETITVREAINGLII
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCEEEHHHHHCEEEE
KSANDAAVTVAEGMKGSEKAFAHLMNIKAKQLGMKNTYFKNASGWHDPLQLTTARDMAKL
ECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCEEEHHHHHHHHH
TMALKKDYPKYYPLFSKTSFVFRGNIVNGHNRVTKNYQGAEGMKTGFHTPAGYNLVTTAS
HHHHHHCCCCCCCCCCCCEEEEECCEECCCCHHCCCCCCCCCCCCCCCCCCCCEEEEECC
RNGKSLIAVVTGGKSAASRDQKMMWLLDQHFNIKPIVHKPLIKLASNNKSTTKAKSKIIK
CCCCEEEEEEECCCCHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
KKNIRS
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]